216 9 Rb1 : 98% ; : :CW-2 245kHz ~1 W 4kHz 5 W ; :722G ; 1 :DZF-62A Figure1 Efectofmicrowavepoweronyieldofgypenosides ; :FA

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1 32 9 OOD & MACHINERY 食品与机械 Vol.32No.9 Sept.216 DOI: /j.issn Optimizationonultrasonic-microwavecombinedextractionof gypenosidesandsynergeticefectanalysis CHENG Yi-qun JI Hang-yan ZHANG Fan HUANG Yi ZHOU Shou-biao HE Rong-rong JI Ya-li CHEN Chun-hua ( 2413) (Colegeof EnvironmentalScienceand EngineeringAnhui Normal UniversityWuhuAnhui2413China) : peratureat62. extractiontimewas12.8min.undertheoptimalex- Box-Be- tractionconditiontheyieldofgypenosideswas24.8±.37 mg/g.the hnken experimentresultsshowedthattheinteractionbetweenultrasonicandmi- 4 crowavewassignificantandplayedamajorroleintheextractionprocess. Keywords:Gynostemma pentaphylum;saponins;ultrasonic-micro- wavecombinedextraction;synergeticefect (Gynostemma pentaphylum) : (5 W) 613 W [1-2] [3] 145s min [4] [5-6] (24.8±.37)mg/g [7-8] [9] [1] [11] [12-13] : ; ; ; [14-15] CO2 Abstract:Optimizationonultrasonic-microwavecombinedextraction ofgypenosidesandtheanalysisonthesynergisticefectbetweenul- trasonicandmicrowavewerestudied.theoptimalextractioncondi- tionofgypenosideswasobtainedbyresponsesurface methodology. Fourfactorsefectontheyieldofgypenosidessuchas microwave powermicrowaveprocessingtimeextractiontemperatureandex- tractiontimewereinvestigated.thesynergisticefectofultrasonic [16-17] andmicrowavewasanalyzedbytheyieldofgypenosidesunderdifer- entextractionconditions.theoptimalextractionconditionofgype- nosidesbyultrasonic-microwavewasultrasonicopen(5 W)micro- wavepowerat613 Wmicrowaveprocessingfor145sextractiontem- 1 : ( :214xmpy14) 1.1 : (1987 ) cheng.sheng515@gmail.com : : ; 135

2 216 9 Rb1 : 98% ; : :CW-2 245kHz ~1 W 4kHz 5 W ; :722G ; 1 :DZF-62A Figure1 Efectofmicrowavepoweronyieldofgypenosides ; :FA min 2min (5 W) 3 [18-19] 2.g 7% 1 2(g/mL) 25mL (1) : 1min min ( W) (2) : 6 W 7 15min (12345 min) (3) : 6W 2min 15min (34567 ) (4) : 6W 2min 6 ( min) DesignExpert8..6 Box-Behnken Figure2 Efectofmicrowaveprocessingtimeonyield ofgypenosides [3] Rb1 : y(od55nm)=2.9275x-.119(r 2 =.9992).4~.2 mg W 6 W Figure3 Efectofextractiontemperatureonyield ofgypenosides 136

3 32 9 : 2 Table2 Experimentaldesignandresults [2] A B C D /(mg g -1 ) min min Figure4 Efectofextractiontimeonyieldofgypenosides ( 1) DesignExpert P < P =.9193> R R 2 Adj : 29 1 (P<.1) (P=.14); (1) 3 ; (P<.1) (2) ( ): 4 Y= A+1.33B+.11C+1.78D-1.5AB- 烄 Y1= A-1.7A 2 ;.49AC +.16AD +.77BC +.12BD +.28CD - Y2= B-1.6B 2 ; 烅 1.7A 2-1.6B 2-1.1C D 2 (1) Y3= C-1.1C 2 ; (2) 1 烆 Y4= D-1.61D 2 5 Table1 Factorsandlevelsofresponsesurface methodologyexperiments A B C D /W /min / /min : 137

4 Table3 ANOVAanalysisfortheresponsesurfacemodified F P A <.1 * * B <.1 * * C D <.1 * * AB <.1 * * AC * * AD BC <.1 * * BD 5.52E E CD 3.19E E 烄 dy/da= a; dy/db= b; 烅 dy/dc= c; 烆 dy/dd= d (3) 6 (3) A <.1 * * B <.1 * * C <.1 * * D <.1 * * < ** (P<.1);* (P<.5) R 2 =.9793R 2 Adj= Figure5 Singlefactorefectcurve Figure6 6 Marginalefectofasinglefactorcurve 6 (3) ( ) : A=.23 B=.416 C=.5 D=.553 ; [21] [22] 7~ Figure7 Efectofmicrowavepowerandmicrowaveprocessingtimeonyieldofgypenosides 138

5 32 9 : 8 Figure8 Efectofmicrowavepowerandextractiontemperatureonyieldofgypenosides 9 Figure9 Efectofmicrowaveprocessingtimeandextractiontemperatureonyieldofgypenosides 8 4 Table4 Analysisofultrasonic-microwave 9 synergisticextraction DesignExpert : W / (mg g -1 ) 613 W 2.44min min 1 145s 24.8±.37 a mg/g min 613 W 145s 613 W min s ±.37 c (24.8±.37)mg/g min 613 W s ±.21 b 12.8min 613 W 4 145s 13.89±.17d min 4 spss18. Duncan s (P<.5) P<.5 4 ; min (P<.5) s 88.5% 71.6% ;

6 216 9 novelsample preparation method for chromatography [J]. JournalofChromatographyA : [5]CHEN Dao-jinLIU Hui-minXINGShao-fangetal.Cytotoxic activityofgypenosidesandgynogeninagainstnon-smalcellung carcinoma A549cels[J].Bioorganic & MedicinalChemistry Leters21424(1): [6]YANGFeiSHIHai-mingZHANG Xiao-weietal.Twonewsap- oninsfromtetraploidjiaogulan (Gynostemma pentaphylum)and theiranti-inflammatoryandα-glucosidaseinhibitoryactivities[j]. [23] FoodChemistry213141(4): [7]MUELLERCGARDENMANN AKEIHOFFGetal.Prevention offreefatyacid-inducedlipidaccumulationoxidativestressandcel deathinprimaryhepatocyteculturesbyagynostemma pentaphylum extract[j].phytomedicine21219(5): [24-25] [8]. [J] (7): s [9]. [J]. 288(1): [1]. [J] (1): [11]. 3 : [12]. [J]. (5 W) 613 W 145s min [13]. [J]. (24.8±.37)mg/g 2925(1): [14]. [J] (2): [15]. HPLC [J] (2): [16]. [D]. : 29:34-4. [17]. (5 W4kHz) - [J] (12): [18]. [J] (33): [1]ZHAO YangXIEZhuo-hongNIU Yu-geetal.Chemicalcompo- sitionshplc/msfingerprinting profilesand radicalscavenging properties of commercial Gynostemma pentaphylum (Thunb.) Makinosamples[J].FoodChemistry212134(1): [2]LIU FangREN DequanGUO De-anetal.Methoddevelop- mentfor gypenosidesfingerprint by high performanceliquid chromatographywithdiode-arraydetectionandtheadditionof internalstandard [J].Chemical & Pharmaceutical Buletin 2856(3): [3]XIE Zhuo-hongLIU WeiHUANG Hai-qiuetal.Chemical compositionoffivecommercialgynostemma pentaphylum sam- plesandtheirradicalscavengingantiproliferativeandanti-in- flammatoryproperties[j].journalof Agriculturaland Food Chemistry2158(21): [4]GANZLER KSALGO AVALKO K.Microwaveextraction:a 14 [J] (18): (3): [19]. Rb1 [J] (6): [2]. [J]. 2633(4): [21]. [J]. 2143(21): [22]. [J]. 2153(7): [23]CRAVOTTO GBOFFA LMANTEGNASetal.Improved extraction of vegetable oils under high-intensity ultrasound and/ormicrowaves[j].ultrasonicssonochemistry2815 (5): [24]. [J]. 26(9):5-8. [25]WANG Yu-tangYOUJing-yanYU Yongetal.Analysisof ginsenosidesinpanax ginsenginhighpressuremicrowave-as- sistedextraction[j].foodchemistry2811(1):

27 3 α- (3) :.2.3() 8U/g α- 3.5%.3%.6%.9%2.2%2.5%.2.3()... (4) α- : 37U/g :.2.3() ( U/g) (5 U/g): 8U/g α-.9% 2.%2.5%3.%3.5%4.%.2.3() :..2 :FW :H

27 3 α- (3) :.2.3() 8U/g α- 3.5%.3%.6%.9%2.2%2.5%.2.3()... (4) α- : 37U/g :.2.3() ( U/g) (5 U/g): 8U/g α-.9% 2.%2.5%3.%3.5%4.%.2.3() :..2 :FW :H OOD & MACHINERY 27 3 食品与机械 Vol.33No.3 Mar.27 DOI:.3652/j.issn.3-5788.27.3.36 Optimizationonpreparationtechnologyofhighlandbarleyenzymolysis liquidsuitbleforprobioticfermentation 2 WAN Ping YI Xiao-cheng