scavenging ability of the bestalkali-soluble extracts of the weakest ultra oxygen free radical scavenging capacity selenium polysaccharide of t

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1 32 4 Vol32 No Journal of Hubei University for NationalitiesNatural Science Edition Dec * ( 1 湖北省地质局第二地质大队, 湖北恩施 ; 2 湖北民族学院生物科学与技术学院, 湖北恩施 ; 3 生物资源保护与利用湖北省重点实验室 ( 湖北民族学院 ), 湖北恩施 ) 以恩施续断为原料, 用蒸馏水 05 mol /L NaCl 75% 乙醇 01 mol /L NaoH 四种溶剂对续断中不同种类硒蛋白进行提取用热水浸提法提取硒多糖, 用原子荧光法测定不同提取物中硒元素的含量, 分析了续断中硒的赋存形态并采用邻苯三酚自氧化法测定续断不同提取物清除超氧阴离子 ( O 2 - ) 的能力, 用 Fenton 法测定其清除羟自由基 ( OH) 的能力, 探究了含硒化合物的抗氧化活性结果表明 : 续断中硒的总含量为 1538 μg /g, 蛋白质多糖中都赋存一定量的硒 ; 蛋白硒是续断中硒的主要赋存形态四种溶剂提取物中碱溶性硒蛋白的含量最高, 它的含量可达总可溶性蛋白的 5728% 按照相同质量样品中结合硒量的多少, 可以得出含硒量 : 总蛋白硒 > 多糖硒 ; 醇溶性硒蛋白 > 盐溶性硒蛋白 > 碱溶性硒蛋白 > 水溶性硒蛋白恩施续断中各种含硒化合物都有一定的清除超氧阴离子和羟自由基的能力, 且不同溶剂提取物的清除率都不同其中醇溶性提取物清除超氧自由基的能力最好, 碱溶性提取物清除超氧自由基的能力最弱 ; 硒多糖清除羟自由基的能力最强续断 ; 硒蛋白 ; 硒多糖 ; 抗氧化活性 S56723 A Study on Combined Forms of Selenium and Antioxidant Activity in Enshi Dipsacus WANG Yingling 1 LI Yanjun 2 3 ZHANG Chi 2 3* 1No2 Geological Brigade of Geological Department of Hubei Province Enshi China 2 School of Biological Science and Technology Hubei University for Nationalities Enshi China 3Key Laboratory of Biologic Resources Protection and Utilization of Hubei Province Hubei University for Nationlities Enshi China AbstractTaking Enshi Dipsacus as raw material different kinds of selenoproteins were extracted with distilled water 05 mol /L NaCl solution 75% ethanol and 01 mol /L NaoHThe hot water extraction method was used to extract selenium polysaccharide the atomic fluorescence spectrometry was used to determine the selenium content in different extracts and the forms of ocuorrence of selenium in Dipsacus were analyzedand pyrogallol Autoxidation method was used to determine the ability of scavenging superoxide anion of different extracts on Dipsacus using fetion eaction determination of the ability of scavenging of hydroxyl radicals Explores the antioxidant activity of organoselenium compounds The results showed that Total content of selenium in Enshi Dipsacus is 1538 μg /g Occurrence in a certain amount of selenium both proteins and polysaccharides but Selenium-protein is the mainly forms of selenium in DipsacusThe highest concentrations of four kinds of solvent extraction is alkali-soluble selenium protein whose content of total soluble protein is 5728%According to the same quality samples in the combined amount of selenium selenium content of order can be calculated asselenoprotein > polysaccharides containing seleniumalcohol-soluble selenoprotein> salt-soluble selenoprotein > alkali soluble selenoprotein > water soluble selenoproteindiffient kind of selenium-containing compounds of Enshi Dipsacus that have a certain degree of superoxide anion and hydroxyl free radical scavenging ability And of different solvent extracts from different clearance rate Alcohol - soluble extracts of which rate is greater than oxygen free radical / CDA011 PKLHB * 1965-

2 scavenging ability of the bestalkali-soluble extracts of the weakest ultra oxygen free radical scavenging capacity selenium polysaccharide of the greatest hydroxyl radical scavenging capacity Key wordsdipsacusselenoproteinselenium polysaccharideantioxidant activity Dipsacus asper Watl % E μg /ml G % 50 mmol /L Tris-HCl ph mol /L PBS ph mol /L PBS ph 74 3 mmol /L 10 mmol /L HCl 075 mmol /L 075 mmol /L FeSO 4 10% TCA 1% K 3 FeCN 6 01% FeCl %H 2 O 2 C 13 GZX-9140 MBE DFY g SZ-93 BS 124S S21-3 LXJ-ⅡB TU-1810 ETHOSPLUS MILESTONE AFS-930d RE g 50 ml 4 h r /min 20 min NH 4 2 SO ml 2 05 mol /L NaCl 50 ml 4 h r /min 20 min NH 4 2 SO 4 05 mol /L NaCl 100 ml 3 75% 50 ml 4 h r /min 20 min min

3 % 100 ml 4 01 mol /L NaOH 50 ml 4 h r /min 20 min ph70 01 mol /L NaOH 100 ml g 40 ml 90 3 h 4 15 min % 85% ml O - 2 %= ΔD 1 /Δt - ΔD 2 /Δt 100 ΔD 1 /Δt D 1 / t D 2 / t Fenton OH 075 mol /L 1 ml ph 74 PBS 2 ml 1 L 075 mmol /L FeSO 4 1 ml H 2 O 2 1 ml min nm D %= D - D 100 D - D D D D H 2 O ml 25 mol /L PBS 20 ml 1% K 3 FeCN 6 20 ml min 10% TCA 20 ml r /min 10 min 20 ml 20 01% FeCl 3 04 ml 10 min 700 nm D D G-250 y = x R 2 = > > > 22 - y = x R 2 = mg /g 23 I = C + 1 Fig1 Protein content of different solvents extraction C μg /gi

4 μg /g 2 > > > 24 2 Fig2 Selenium content of different solvents extraction Fig3 The clearance rate of superoxide anion from different extracts Fenton D 4 > > > 26 4 Fig4 The clearance rate of Hydroxyl free Fe 3+ Fe 2+ radicals from different extracts 5 5 > > > 3 5 Fig5 The comparison of reduction ability between extracts with different solvents 1314 mg /g 0258 mg /g > > > 1677 mg /g μg /g 42 76% > > > > 22642% 8542% > > > 0801

5 I M Ⅵ Ⅵ ( 上接第 370 页 ) 5Yamamoto I Nagumo T Takahashi M et alantitumor effect of seaweeds III Antitumor effect of an extract from Sargassum kjellmanianum Japan Journal of Experimental Medicine Melo F R Pereira M S Foguel D et alantithrombin-mediated anticoagulant activity of sulfated polysaeeharides-different mechanisms for heparin and sulfated galactans Journal of Biological Chemistry Nishino T Nagumo TThe sulfate-content dependence of the anticoagulant activity of a faun sulfate from the brown seaweed Ecklonia kurome Carbohydrate Research l Matsubara K Mori M Mstsumoto H et alantieoagulant properties of a sulfated galactan isolated from a marine green algacodium cylindricum Journal of Applied phycology

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核农学报２０１６３０５０９２００９２５ＪｏｕｒｎａｌｏｆＮｕｃｌｅａｒＡｇｒｉｃｕｌｔｕｒａｌＳｃｉｅｎｃｅｓ９２０文章编号１０００８５５１２０１６０５０９２００６羊栖菜活性组份多糖的体外抗氧化能力比较丁浩淼１２李伟强１汪财生 1 顾筱筱１谢琰１钱国英１王忠华１１浙江万里学院生物与环境学院浙江宁波３１５１００２浙江海洋高效健康养殖协同创新中心