Microsoft Word - 10-崔嘉成_new_.doc

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1 作物学报 ACTA AGRONOMICA SINICA 203, 39(0): ISSN ; CODEN TSHPA9 DOI: /SP.J 甘蓝型油菜裂角相关性状的遗传与相关分析崔嘉成王军,2,2,3 刘胡佳琼,* 梅德圣李云昌付丽彭鹏飞,2 / /, ; 2, 0008; 3, :, ,,,,,, ;, ZSB R, : ; ; ; Genetic and Correlation Analysis on Pod Shattering Traits in Brassica napus L. CUI Jia-Cheng,2, LIU Jia, MEI De-Sheng, LI Yun-Chang, FU Li, PENG Peng-Fei,2, WANG Jun,2,3, and HU Qiong,* Oil Crops Research Institute of Chinese Academy of Agricultural Sciences / National Center for Oil Crops Improvement / Key Laboratory for Biological Sciences and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan , China; 2 Graduate School of Chinese Academy of Agricultural Sciences, Beijing 0008, China; 3 Oil Crops Research Institute of Guizhou Province, Guiyang , China Abstract: Pod shattering resistance is a very important trait of oilseed rape, and the genetic research has been extremely weak. In this study, pod shattering resistance index (SRI) and other seven pod traits were analyzed based on data collected from a 6 5 incomplete diallel design at two environments. The results demonstrated that obvious genetic variation existed among elite breeding lines and their crosses for pod SRI. Pod shattering resistance was likely controlled by few genes with mainly additive effects, and dominant effects were much less important, whereas the environments played only minor role for pod SRI. There was no significant heterosis in most of the crosses. Pod SRI was detected to be significantly positively correlated with pod length, pod wall weight, 000-seed weight and seed diameter, but negatively correlated with pod density and number of seeds per pod. The genetic variations of pod length, 000-seed weight, pod density and seed diameter were mainly explained by additive effects, whereas those of pod wall weight and number of seeds per pod were mainly affected by dominant effects. Among the elite breeding lines, ZSB and R were recommended for using as direct parental lines for making pod shattering resistant hybrid varieties as maintainer and restorer lines for Polima cytoplasmic male sterility system, respectively, due to their higher general combining ability on pod SRI and other yield component traits. Keywords: Oilseed rape; Genetic effect; Pod shattering resistance index; Correlation analysis [], [2], (973 )(20CB09302), (863 )(202AA007), (CARS-3), (200BAD0B02) * (Corresponding author):, huqiong0@caas.cn, Tel: : cuijiacheng2008@63.com Received( ): ; Accepted( ): ; Published online(): URL:

079 甘蓝型油菜裂角相关性状的遗传与相关分析崔嘉成王军,2,2,3 刘胡佳琼,* 梅德圣李云昌付丽彭鹏飞,2 / /, 430062; 2, 0008; 3, 550006 :, 30 2 7,,,,,, ;, ZSB R, : ; ; ; Genetic and Correlation Analysis on Pod

2 792 39, [3], 0% [4], 20% [5], 50% [6-7],, 3,,,,,,, Szot [8] SPODNAM 0%~45%,,,,,, [9] [3] [0],,,, 2,,,,,, [3,-2], [3], Morgan [4],,, ;,, [5] 229 ( ),,,,, ( ), [4,5],,, F, - [6],, 材料与方法., B 055B 6098B 8908B ZSB ( )( ) HPR R2 R6 R9 R (), ( ), ZSB, 6, 5,, 30 F,, (E )(E 2 ), 3, 4, 2 m, 0.33 m, 8 0,, 0, 20, 200, 20, 4.8 cm 7.4 cm, 8 4 mm, 280 min 24 mm (HQ45Z,

方法., 0028 09B 055B 6098B 8908B ZSB ( )( ) HPR R2 R6 R9 R (), ( ), ZSB, 6, 5,, 30 F,, 4.

3 0 : 793 ) 0 min, 2 min, 3, = x i (6 i)/00( ),, x i i, i 5; = 00, SC-I,.3 - [6], QGAStation ( qga/), Jackknife, SAS 2 结果与分析 2. 4, 2 F, 79, (P<0.000), ( ), 79 0~0.47, 0.28, 82.95%,,, 0, , 0.382; 0, , ( ) 0.92 (P<0.000),, , 055B ZSB, 09B R, F, F 0.02~0.27, 0.0; 0.0~0.33, 0.; F, (P<0.000), Source Table df 表抗裂角指数的方差分析 ANOVA of pod shatter resistance index SS MS F F-value Block Location Line <0.000 Line location Error Total P > F Fig. 图个亲本及 30 个杂交组合的抗裂角指数 Pod shatter resistance index of parents and 30 crosses

000), Source Table df 表抗裂角指数的方差分析 ANOVA of pod shatter resistance index SS MS F F-value Block 2 0.0033 0.007 0.69 0.5042 Location 0.007 0.007 0.72 0.3989 Line 40.3290 0.

4 F, 055B R, R 09B R 6098B R 8908B R ZSB R2 ZSB R9, ,, QGAStation, 7 ra () rd ( ) rp ( ) rg () 4,,, ( 2),, 0.45 >> > >> >,,,,, 0.606,,,, ( 3),,, 50% 4,,, 40%; Parameter Pod length Table 2 Beak length 表 2 抗裂角指数与角果性状间相关系数 Correlation coefficient between pod SRI and other pod traits Pod wall weight Seeds per pod 000-seed weight Pod number per centimeter Seed diameter ra ** ** ** ** ** ** rd ** ** rp ** ** 0.89 ** 0.23 ** ** ** rg ** ** ** 0.30 ** ** ** *, ** : 5% % * Significant at P<0.05, ** Significant at P<0.0. Table 3 表 3 油菜角果相关性状方差分量比率估算值 Estimated proportions of variance components for pod traits Beak Pod wall 000-seed Pod per centimeter diameter Seed Parameter Pod length Seeds per pod Pod SRI length weight weight V a /V p ** ** ** ** ** ** 0.55 ** ** V d /V p 0.8 ** ** ** ** 0.45 ** ** * V ae /V p ** ** ** ** V de /V p 0.54 ** ** ** ** 0.05 ** V e /V p ** 0.23 ** 0.66 ** ** ** ** ** ** *, ** : 5% %V a : ; V d : ; V p : ; V ae : ; V de : ; V e : * Significant at P<0.05, ** Significant at P<0.0. V a : additive variance; V d : dominant variance; V p : phenotypic variance; V ae : additive and environment interaction variance; V de : dominant and environment interaction variance; V e : error variance.

weight Pod number per centimeter Seed diameter ra 0.606 ** 0.037.000 ** 0.479 ** 0.383 ** 0.339 ** 0.460 ** rd 0.046 ** 0.007 0.94 0.52 0 0.03 ** 0.290 rp 0.405 ** 0.006 0.375 ** 0.89 ** 0.23 ** 0.

5 0 : 795,, ( 3), (V a /V p +V d /V p ) 55%,, 87.2%, 80%,, 60%,, 2.4,,,, 8, 2 4, 6, 055B, 0.0, ZSB R 09B, ;, R6 (0.083), R2 HPR R B, 0.07~0.042 R9 6098B,, 8908B 6 (, ), 4, ZSB,, 055B,,, 2, R, 09B 2.5,,,,, 20%, 30, 7, 0.07~0.086 ( 5) 4 (0028 R ZSB R HPR 055B R9), 055B R9 2, 2 Parent Pod SRI Table 4 Pod length 表 4 亲本各性状加性效应预测值 Predicted additive effects of different pod traits for parent lines Pod wall weight Seeds per pod 000-seed weight Pods per centimeter Seed diameter ** ** ** * 09B ** ** 0.05 * ** ** 0.03 * 055B 0.0 ** **.272 ** 0.9 ** 0.36 ** B 0.07 * ** 0.03 **.325 ** ** * ** 8908B * ** * 0.88 ** 0.02 ZSB 0.08 **.39 ** 0.03 ** ** ** HPR ** ** ** 0.77 ** ** 0.05 ** R ** * 0.58 * R ** 0.37 * * R * * ** 0.83 ** 0.70 ** ** R ** ** **.59 ** ** ** ** *, ** : 5% % * Significant at P<0.05; ** Significant at P<0.0.

086 ( 5) 4 (0028 R ZSB R2 0028 HPR 055B R9), 055B R9 2, 2 Parent Pod SRI Table 4 Pod length 表 4 亲本各性状加性效应预测值 Predicted additive effects of different pod traits for parent lines Pod wall

6 Cross 表 5 部分 F 杂交组合抗裂角指数的显性效应预测值 Table 5 Predicted dominance effects of pod SRI for some F crosses D ij D ij E D ij E 2 ZSB R ** * 055B R ** B R * R * ZSB R * * 0028 HPR 0.07 * * B R * *, ** : 5% %D ij : i j ; D ij E : i j ; D ij E 2 : i j 2 * Significant at P<0.05; ** Significant at P<0.0. D ij : dominance effect of i j; D ij E : dominance effect of i j at location ; D ij E 2 : dominance effect of i j at location 2. 4, ZSB R2, 0028 HPR, ZSB R 09B R [7], F , 0.004~0.293; 0.87,.060~0.480; 0.873, 2.880~0.08 4, 2, 0028 HPR ZSB R2,, (09B R) (0028 HPR), 5 6, 4 3 讨论,,, Mongkolporn [8] 2 [9] F 2 2 QTL [20], 9 4 QTL, 45.9% 38.6%, Hu [2], QTL, 47% 2,,,,,,,,, Morgan [4],, [22] [23] Morgan [2] Wang [3] [5],,,,,,

29 *, ** : 5% %D ij : i j ; D ij E : i j ; D ij E 2 : i j 2 * Significant at P<0.

7 0 : 797, [5], Wang [3] (r=0.59),, [5],,, Wang [3], [5] Morgan [2], [23], [23], 3,,, [24],,,,,,, () 2 055B ZSB ZSB,, ZSB, ZSB R2 ZSB R 055B,,,,, ZSB, R,, () 4 结论,,, ; ;, ZSB, R 致谢 : 感谢浙江大学朱军教授举办的数量遗传分析及 QTL 定位研讨班并无偿提供 QGAstation 数据分析软件 ; 感谢安红玉桑世飞康中德对性状鉴定的贡献 References [] Østergaard L, Kempin S A, Bies D, Klee H J, Yanofsky M F. Pod shatter-resistant Brassica fruit produced by ectopic expression of the FRUITFULL gene. Plant Biotechnol J, 2006, 4: 45 5 [2] Morgan C L, Bruce D M, Child R, Ladbrooke Z L, Arthur A E. Genetic variation for pod shatter resistance among lines of oilseed rape developed from synthetic B. napus. Field Crops Res, 998, 58: [3] Wang R, Ripley V L, Rakow G. Pod shatter resistance evaluation in cultivars and breeding lines of Brassica napus, B. juncea and Sinapis alba. Plant Breed, 2007, 26: [4] Peng P-F(), Li Y-C(), Hu Q(), Liu F-L( ), Li Y-D(), Xu Y-S(), Mei D-S(). Screen of varieties suitable for machine harvesting from new breeding hybrids or lines in Brassica napus. Acta Agric Boreali- Sin (), 2009, 24(6): (in Chinese with English abstract) [5] Price J S, Hobson R N, Neale M A, Bruce D M. Seed losses in commercial harvesting of oilseed rape. J Agric Eng Res, 996, 65: 83 9 [6] Kadkol G P, Macmillan R H, Burrow R P, Halloran G M. Evaluation of Brassica genotypes for resistance to shatter: I. Development of a laboratory test. Euphytica, 984, 33: [7] Child R D, Evans D E. Improvements of recoverable yields in oilseed rape (Brassica napus) with growth retardants. Aspects Appl Biol, 989, 23: [8] Szot B, Tys T. The influence of the Spodnam Dc preparation on agrophysical properties of rape siliques and seed losses at matu-

定位研讨班并无偿提供 QGAstation 数据分析软件 ; 感谢安红玉桑世飞康中德对性状鉴定的贡献 References [] Østergaard L, Kempin S A, Bies D, Klee H J, Yanofsky M F.

8 ration and harvest. Proceedings of the 8th International Rapeseed Congress, 99, pp [9] Wen Y-C(), Fu T-D(), Tu J-X(), Ma C-Z(), Shen J-X(), Zhang S-F(). Advances in studies of pod shattering resistance in rapeseed. J Plant Genet Resour (), 2009, 0(): (in Chinese with English abstract) [0] Summers J E, Bruce D M, Vancanneyt G, Redig P, Werner C P, Morgan C, Child R D. Pod shatter resistance in the resynthesized Brassica napus line DK42. J Agric Sci, 2003, 40: [] Roberts J A, Elliott K A, Gonzalez-Carranza Z H. Abscission, dehiscence, and other cell separation processes. Annu Rev Plant Biol, 2002, 53: 3 58 [2] Kadkol G. Brassica Shatter-Resistance Research Update. 6th Australian Research Assembly on Brassicas, 2009 [3] Wei W h, Li Y C, Wang L J, Liu S Y, Yan X H, Mei D S, Li Y D, Xu Y S, Peng P F, Hu Q. Development of a novel Sinapis arvensis disomic addition line in Brassica napus containing the restorer gene for Nsa CMS and improved resistance to Sclerotinia sclerotiorum and pod shattering. Theor Appl Genet, 200, 20: [4] Morgan C L, Ladbrooke Z L, Bruce D M, Child R, Arthur A E. Breeding oilseed rape for pod shattering resistance. J Agric Sci, 2000, 35: [5] Wen Y-C(), Fu T-D(), Tu J-X(), Ma C-Z(), Shen J-X(), Zhang S-F(). Screening and analysis of resistance to silique shattering in rape (Brassica napus L.). Acta Agron Sin ( ), 2008, 34(): (in Chinese with English abstract) [6] Zhu J(). New approaches of genetic analysis for quantitative traits and their applications in breeding. J Zhejiang Univ (Agric & Life Sci)( ), 2000, 26(): 6 (in Chinese with English abstract) [7] Wu J-X(), Zhu J(). Methods for predicting genotypic value and heterosis of crop hybrids offspring at different environments. J Zhejiang Agric Univ (), 994, 20(6): (in Chinese with English abstract) [8] Mongkolporn O, Kadkol G P, Pang E C K, Taylor P W J. Identification of RAPD markers linked to recessive genes conferring silique shatter resistance in Brassica rape. Plant Breed, 2003, 22: [9] Peng P-F(). Determination, Genetic Analysis and QTL Mapping of Pod Shatter Resistance in Rapeseed (Brassica napus L.). MS Thesis of Chinese Academy of Agricultural Sciences, 2009 (in Chinese with English abstract) [20] Wen Y-C(). Analysis of Silique Shatter Resistance and Its QTLs Mapping in Brassica napus L. PhD Dissertation of Huazhong Agricultural University, 202 (in Chinese with English abstract) [2] Hu Z Y, Hua W, Huang S M, Yang H L, Zhan G M, Wang X F, Liu G H, Wang H Z. Discovery of pod shatter-resistant associated SNPs by deep sequencing of a representative library followed by bulk segregant analysis in rapeseed. PloS ONE, 202, 7: 7 [22] Zuo Q-F(), Zhu J(), Liu Y-B(), Pan X-Y( ), Zhang J-Z(). Genetic analysis of genotype environment interaction for yield components of rice (Oryza sativa L.) in unequal experiment design. Acta Agron Sin ( ), 200, 27(4): (in Chinese with English abstract) [23] He Y-T(), Li D-R(). A preliminary study of pod shatter resistance in hybrid Brassica napus L. Shaanxi J Agric Sci (), 996, (3): 30 3 (in Chinese) [24] Wu J-X(), Wang G-J(), Zhu J(), Xu F-H( ), Ji D-F(). Genetic analysis on direct and maternal effects of seed traits in upland cotton (Gossypium hirsutum L.). Acta Agron Sin ( ), 995, 2(6): (in Chinese with English abstract)

J Plant Genet Resour (), 2009, 0(): 40 45 (in Chinese with English abstract) [0] Summers J E, Bruce D M, Vancanneyt G, Redig P, Werner C P, Morgan C, Child R D.

Microsoft Word - 17--刘秀秀_new_.doc

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