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1 作物学报 ACTA AGRONOMICA SINICA 2017, 43(6): ISSN ; CODEN TSHPA9 DOI: /SP.J 陆地棉高秆突变体的激素变化与 Tp 基因的染色体定位陈旭升狄佳春周向阳赵亮 /, : 1, N099, 24 h, 1 3 GA 3 ZR IAA, GA 3 F 1 : GA 3, ZR IAA 1 GA 3 ; ZR IAA, 4 SSR Tp, NAU2083 NAU4045 NAU2419 NAU4044, Tp NAU4045 NAU2419, 7.4 cm 41.2 cm, Tp Chr.1 : ; ; ; Tp ; Hormone Expression and Tp Gene Chromosomal Localization of Tall Plant Mutant from Upland Cotton CHEN Xu-Sheng, DI Jia-Chun, ZHOU Xiang-Yang, and ZHAO Liang Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences / Key Laboratory of Cotton and Rape in the Lower Reaches of the Yangtze River, Ministry of Agriculture, Nanjing , China Abstract: Taking a tall plant mutant Gaogan 1 as test material, and a common variety N099 as control, three hormones from soaked seed embryos were determined with enzyme linked immunosorbent assay. The contests of GA 3, ZR, and IAA in the mutant were respectively higher than those in the normal plant, especially the GA 3 content was significantly higher. GA 3 contents in F 1 individuals were significantly higher than those in two parents, but ZR and IAA contests were even lower than those in normal plant parents, which showed that the mutant is a GA 3 enrichment-type mutant. F 2 plants from a cross of Gaogan 1 N099 were used to map the mutant gene Tp, showing four molecular markers including NAU2083, NAU4045, NAU2419, and NAU4044 linked with Tp gene. The Tp gene flanked with molecular markers NAU4045 and NAU2419, with the genetic distance of 7.4 cm and 41.2 cm, respectively. Thus, Tp gene is located in cotton Chr. 1. Keywords: Upland cotton; Tall plant mutant; Hormone; Tp gene; Chromosomal localization, [1-2] [3-5], [6-9], [10-13], [14],, Tp, 1,,, [15] F 1, SSR Tp, 1 材料与方法 N099 F 1 30, h, 0.1 g, (ELISA) 3 (2016ZX , 2016ZX ) This study was supported by the National Major Project for Developing New GM Crops (2016ZX , 2016ZX ). : njcxs@126.com, Tel: , Received( ): ; Accepted( ): ; Published online( ): URL:

936 43 (GA 3 ) (IAA) (ZR), 3 1.2, 1 N099 F 1 F 2,, 2, 2, 20, 1.

2 (GA 3 ) (IAA) (ZR), 3 1.2, 1 N099 F 1 F 2,, 2, 2, 20, 1.3, Paterson s [16] DNA F 2 10, DNA, SSR [17], F 2, F 2 SSR PCR PTC-200 (MJ research) PCR 94 2 min; s; s; 72 1 min, 30 ; 72 7 min 8.0% (PAGE) : 1 TBE, 200 V, [18] : 1, 2, 3, 0 JoinMap 4.0, Tp 2 结果与分析 cm, ,, 1 N099 (119.5±10.5) cm (72.0±10.4) cm, F 2 (B) (A), 2,, 2 图 1 F 2 分离群体的典型高秆株 (B) 与正常株 (A) Fig. 1 Typical tall plant (A) and normal plant (B) from F 2 segregation population , 1 GA 3 ZR IAA, GA 3 ; ZR IAA, 1 N099 F 1 GA 3, ZR IAA 1 GA 3, ZR IAA 2.3 Tp : F 1, F 2 =3 1 ( 2), SSR F 2 DNA 234, 3, NAU2083 ( Chr.1) NAU1042 ( Chr.5) NAU3368 ( Chr.20) F 2, 1 NAU2083 Tp, 7.2 cm Chr.1 NAU cm 22 SSR 5, 22.7% F 2, JionMap 4.0, 4 Tp, NAU2083 NAU4045 表 1 高秆 1 号及杂交 F 1 种胚中内源激素含量 Table 1 Endogenous hormone content in embryos of tall parent and its hybrid F 1 (ng g 1 ) Material GA 3 ZR IAA 1 Gaogan b 23.5 a a [ 1 N099]F 1 [Gaogan 1 N099]F a 12.2 b b N099 ( ) Normal height 7.0 c 17.8 a ab (P 0.05) Values followed by different letters are significantly different at P < 0.05.

3 6 : Tp 937 Generation Table 2 表 2 高秆突变体与常规株高品种杂交后代的性状表现 Plant height traits in hybrid offspring of a tall plant mutant crossing with normal plant variety Parent and cross Tall plant Normal plant χ 2 χ 2 value of expected ratio BC 2 1:1/F 2 3:1 Probability P 1 1 Gaogan Whole tall plant P 2 N099 ( ) Normal plant 0 46 Normal plant F 1 1 N099 Gaogan 1 N Whole tall plant F 2 [ 1 N099] Gaogan 1 N099 selfcross NAU2419 NAU4044 Tp NAU4045 NAU2419, 7.4 cm 41.2 cm ( 2), Tp Chr.1 图 2 高秆基因 Tp 在 Chr.1 的连锁图谱 Fig. 2 Linkage map of tall plant gene Tp in Chr.1 3 讨论 3.1 Chr.1, (v 5 ) (L L 1 ) (lp 1 ) [19], [14] Tp, F 1, F 1 [20], 5% ;,, Bt, [21] GA 3 ZR IAA,, GA 3 ; ZR IAA F 1, GA 3, ZR IAA, GA 3 ; F 1, GA 3 3.2, QTL Shappley [22] F 2 2 QTL, 6 23 [23] F 2:3, 2 QTL, Chr.2 Chr.11, 9.18% 9.48% [24] 36 1,, QTL, 3 QTL 2 ( Chr.1 Chr.3 Chr.17), 3 QTL 3 ( Chr.14 Chr.20 Chr.25), 1 QTL 4 ( Chr.14),, F 2 F 2, SSR, Tp 1, 1 [25-26],, Chr.1, :, 30 cm, 1, References [1],,,,.., 1995, 21: Xu Q Z, Wang Q C, Niu Y Z, Wang Z X, Zhang J. Studies on relationship between plant type and canopy photosynthesis in

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