王芙蓉, 等 : 牛磺酸对大鼠铁利用和抗氧化能力的影响 when adding iron in the same time. Adding taurine had no significant effects on iron utilization and an

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1 WANG Fu-rong,et al: Effects of Taurine on Iron Utilization 王芙蓉 1,2, 谢中国 1, 叶兴乾 2, 邓尚贵 3, 陈士国 *2, 胡亚芹 2, 肖定福 4 (1., ; 2., ; 3., ; 4., ) : 研究了牛磺酸对缺铁性贫血大鼠和正常大鼠铁利用和抗氧化能力的影响通过对 SD 大鼠喂养缺铁饲料, 建立缺铁性贫血大鼠模型, 再给缺铁性贫血大鼠和正常大鼠灌胃 0.5 g/kg 牛磺酸共 21 d, 比较牛磺酸对正常大鼠和缺铁性贫血大鼠铁利用和抗氧化能力的影响结果表明, 牛磺酸可显著提高缺铁性贫血大鼠血红蛋白含量, 提高血清铁和铁蛋白含量, 提高肝脏铁含量, 降低血清总铁结合力 (P < 0.05); 可提高缺铁性贫血大鼠抗氧化能力, 在补铁的同时添加牛磺酸效果更佳添加牛磺酸对正常大鼠铁利用和抗氧化能力的提高效果不显著 (P > 0.05) 因此, 牛磺酸对缺铁性贫血大鼠铁的利用和抗氧化能力的提高效果优于正常大鼠 : 牛磺酸 ; 大鼠 ; 缺铁性贫血 ; 铁利用 ; 抗氧化 :Q 517;Q :A : (2013) Effects of Taurine on Iron Utilization WANG Fu-rong 1,2, XIE Zhong-guo 1, YE Xing-qian 2, DENG Shang-gui 3, CHEN Shi-guo *2, HU Ya-qin 2, XIAO Ding-fu 4 (1. Zhejiang Zhoushan Putuo Marine High Technology Zone, Zhoushan , China; 2. College of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou , China; 3. College of Food and Pharmacy&medicine, Zhejiang Ocean University, Zhoushan , China; 4. College of Animal Science and Technology, Hunan Agricultural University, Changsha ,China) Abstract: The aim of this manuscript was to investigate the effects of taurine on iron utilization and antioxidative ability of iron deficiency anemia (IDA) rats and normal rats,for those,the iron utilization and antioxidative ability in IDA rats and normal rats were determined after gavaged 0.5 g/kg taurine for 21 days. The IDA rat model was established through fed iron deficiency feed to the SD rats Hemoglobin,serum iron,serum ferritin,total iron binding capacity and the content of liver and spleen iron,mda,sod,gsh-px and T-AOC were determined respectively after the end of the intervention. It was found that the addition of taurine in IDA rats increased hemoglobin,serum iron,serum ferritin,the content of liver iron and decreased the total iron binding capacity significantly (P < 0.05). Adding taurine improved the antioxidative ability in IDA rats, especially : : (2011C22026); 2012 : (1982 ),,,, * : (1982 ),,,,, chenshiguo210@163.com 貐貛貧

5 g/kg 牛磺酸共 21 d, 比较牛磺酸对正常大鼠和缺铁性贫血大鼠铁利用和抗氧化能力的影响结果表明, 牛磺酸可显著提高缺铁性贫血大鼠血红蛋白含量, 提高血清铁和铁蛋白含量, 提高肝脏铁含量, 降低血清总铁结合力 (P < 0.

2 王芙蓉, 等 : 牛磺酸对大鼠铁利用和抗氧化能力的影响 when adding iron in the same time. Adding taurine had no significant effects on iron utilization and antioxidative ability in normal rats (P > 0.05). Therefore,the effects of taurine on iron utilization and antioxidative ability in the IDA rats were better than that of the normal rats. Key words: taurine, rat, iron deficiency anemia, iron utilization, antioxidant, ((24±1),(55±5)%),,, 12 h/d,,, 40, (IDA) IDA, 10 ml,, Hb,,IDA 10%~30% [1] IDA, [2] 1.3, 血液指标的测定 Hb : [3-4] [9], ; SI TIBC, [5], SF ELASA, [6-7], R&D, 肝脏和脾脏中铁含量的测定 (V( ) V( )=4 1) 1 1.1, 99%;FeSO4.7H2O, ;SD 血清抗氧化指标的测定 (T-, ;UV-2550 AOC) (SOD), Shimadzu ;AA SD 40, (80±5) g, 3 d 4, 16 (SI) (SF) (TIBC) 10 ml,,,, Perkin Elmer AA700 (GSH-PX) (MDA), Perkin Elmer 1.4 SPSS18.0, Duncan, 24, 28 d [8] AIN-76, 2 Hb 3 : d, Hb (86.07±3.40)g/L, d, + Hb 0.5 g/kg,, (P < 0.05),, 21 d 3 (P < 0.05), SI SF 貐貛貨

Key words: taurine, rat, iron deficiency anemia, iron utilization, antioxidant, ((24±1),(55±5)%),,, 12 h/d,,, 40, (IDA) IDA, 10 ml,, Hb,,IDA 10%~30

3 WANG Fu-rong,et al: Effects of Taurine on Iron Utilization (P < 0.05), + SI SF + SI SF (P > (P < 0.05), + SI SF 0.05) TIBC 4 (P < + (P < 0.05), 0.05) Table 1 1 Effect of taurine supplements on blood indexs of rats Hb/(g/L) SI/(mg/L) SF/(ng/ml) TIBC/(mg/L) ±13.07 c 16.34±1.19 c ±5.71 d 28.84±1.10 a ±5.00 c 16.42±0.82 c 107.5±5.63 d 28.74±1.04 a 83.88±6.3 a 9.73±1.43 a 25.52±2.71 a 35.43±1.43 c ±6.03 b 13.44±0.78 b 47.96±4.02 b 31.77±1.01 b ±7.57 c 16.40±0.48 c 84.79±2.05 c 28.79±0.86 a a,b (P < 0.05) 2.2 T-AOC SOD GSH-PX, MDA, + MDA 2 SOD (P < (P < 0.05) 0.05),2 (P < 0.05), Table 2 Effects of taurine on iron contents of liver and, + spleen of rats /(mg/kg) /(mg/kg) (P < 0.05) ±23.02 c ± b ±21.77 c ±61.27 c T-AOC SOD GSH-PX ±38.92 a ±22.28 a, MDA (P < 0.05), ±28.17 b ±84.44 a ±30.67 c ±57.72 bc a,b (P < 0.05) (P > 0.05) + Table 3 3 Effects of taurine on serum antioxidative ability of rats T-AOC/(U/mL) MDA/(nmol/L) SOD/(U/mL) GSH-PX/(U/mL) 14.22±3.64 ab 5.49±0.99 ab ±74.55 b ±76.69 ab ±3.26 b 5.07±0.86 a ±54.06 b ±69.92 b 11.40±1.66 a 7.66±1.76 c 338.8±68.18 a ±32.46 a ±1.39 ab 6.71±0.89 bc ±30.81 ab ±34.06 ab ±1.72 ab 6.07±0.92 ab ±78.95 b ±81.13 ab a,b (P < 0.05) 2.4 Hb, Hb,, 貐貛販

5±5.63 d 28.74±1.04 a 83.88±6.3 a 9.73±1.43 a 25.52±2.71 a 35.43±1.43 c + 108.00±6.03 b 13.44±0.78 b 47.96±4.02 b 31.77±1.01 b + 134.46±7.57 c 16.40±0.48 c 84.79±2.05 c 28.79±0.86 a a,b (P < 0.05) 2.

4 王芙蓉, 等 : 牛磺酸对大鼠铁利用和抗氧化能力的影响 SF,, IDA MDA SOD [10] TIBC, GSH-Px T-AOC, IDA [11] IDA SI SF,,TIBC, SI SF,TIBC,, IDA,, IDA, IDA,, + IDA IDA SOD GSH-Px, 3, MDA, MDA, [12],,, T-AOC SOD GSH-Px MDA,, MDA, SOD GSH-Px T-AOC, IDA,, [13], ; : [ 1 ] WHO/UNI CEF/UNU. Iron deficiency anaemia:assessment prevention,and control[m]. Geneva:World Health Organization, [ 2 ] Srigiridlhar K,Madhavan N K. Iron-deficient intestine is more susceptible to peroxidative damage during iron supplementation in rats[j]. Free Radical Biology and Medicine,1998,25(6): [ 3 ],,,. [J].,2011,30(2): WANG Fu -rong,tong Jian -ming,zhang Xiao -ming,et al. Effects of dietary taurine on egg production,egg quality and cholesterol levels in Japanese Quail[J]. Journal of Food Science and Bitechnology,2011,30(2): (in Chinese) [ 4 ],,,. [J].,2010,29(3): WANG Fu-rong,TONG Jian-ming,ZHANG Xiao-ming,et al. Effects of dietary taurine on egg production,immune responses and fat metabolism in laying quails[j]. Journal of Food Science and Bitechnology,2010,29(3): (in Chinese) [ 5 ],,,. [J].,2005,9: LI Qiu-lian,GAO Qiu-hua,XUE Cheng-bin,et al. Effects of taurine on growth and anaemia in rats exposed to lead[j]. Chinese Pharmaceutical Journal,2005,9: (in Chinese) [ 6 ] Oudit G Y,Trivieri M G,KhaPer N,et al. Taurine supplementation reduces oxidative stress and improves cardiovscular function in an iron-overload murine model[j]. Circulation,2004,109:1877-l885. [ 7 ],,,. C [J]. :,2011,51 (11): 貐貛貪

[ 2 ] Srigiridlhar K,Madhavan N K. Iron-deficient intestine is more susceptible to peroxidative damage during iron supplementation in rats[j]. Free Radical Biology and Medicine,1998,25(6):660-665.

5 WANG Fu-rong,et al: Effects of Taurine on Iron Utilization ZENG Ya-juan,XU Qun-ying,FENG Jian-gao,et al. Effects of Vitamin C and taurine on iron overload cardiomyopathy in mice [J]. Journal of Nanchan University:Medical Science,2011,51(11):1-5. (in Chinese) [ 8 ] Borel M J,Smith S H,Brigham D E,et al. The impact of varying degrees of iron nutrition on several functional consequences of iron deficiency in rats[j]. Journal of Nutrition,1991,121: [ 9 ] Davidsohn I,Nelson D A. The blood [G]//Davidsohn I,Henry J B,Saunders W B,Clinical Diagnosis by Laboratory Methods. Philadelphia:PA. 1974; [10] Cook J D. Diagnosis and management of iron -deficiency anaemia [J]. Best Practice & Research Clinical Haematology, 2005,18: [11] Clark S F. Iron deficiency anemia:diagnosis and management[j]. Current Opinion in Gastroenterology,2009,25: [12] Slater T F. Free radicals mechanisms in tissue injury[m]. [S.I.]:Cell Function and Disease,1989: [13] Uehara M,Chiba H,Mogi H,et al. Introduction of increased phosphatidylcholine hydroperoxide by an iron-deficient diet in rats [J]. Journal of Nutritional biochemistry,1997(8): FDA , (FDA), the International Dairy Foods Association (IDFA) the National Milk Producers Federation (NMPF) FDA,, (reduced calorie) (no added sugar), FDA, (reduced calorie), FDA 3, 5 21 [ ]U.S. Food and Drug Administration. FDA Wants Your Opinion on Dairy-Product Labels [EB/OL]. ( ) , 2 / DSM,, 3 2-3, ( DHA), ( EPA),, EPA DHA 5 / [ ]. 2 [EB/OL]. ( ). http: // 貐貜財

The impact of varying degrees of iron nutrition on several functional consequences of iron deficiency in rats[j]. Journal of Nutrition,1991,121:729-36. [ 9 ] Davidsohn I,Nelson D A.

中草药 Chinese Traditional and Herbal Drugs 第 46 卷第 9 期 2015 年 5 月 1339 非酒精性脂肪性肝病 (nonalcoholic fatty liver disease,nafld) 是一种与胰岛素抵抗遗

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王 芙 蓉, 等 : 牛 磺 酸 对 大 鼠 铁 利 用 和 抗 氧 化 能 力 的 影 响 when adding iron in the same time. Adding taurine had no significant effects on iron utilization and an

王芙蓉, 等 : 牛磺酸对大鼠铁利用和抗氧化能力的影响 when adding iron in the same time. Adding taurine had no significant effects on iron utilization and an