Microsoft Word - 7——聂鸿涛_news_.doc

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1 不同温度和盐度对大竹蛏耗氧率和排氨率的影响 1, 聂鸿涛 2 1,, 姜力文 2, 王海伦 1, 邢宁宁 1 1,, 杨凤 2 1, 2, 闫喜武 (1., ; 2., ) 摘要 : 在实验室条件下, 设置和 32 五个温度梯度和和 32 五个盐度梯度, 研究了不同温度和盐度对大竹蛏 (Solen grandis) 耗氧排氨的影响结果表明 : 随着温度的升高耗氧率先降低再逐渐升高, 在温度为 22 时为最小值 mg/(g h); 排氨率先降低后升高再降低, 在温度 17 时为最小值 mg/(g h) 随着盐度增加耗氧率先升高后降低, 在盐度 22 时达到最大值 mg/(g h), 排氨率先降低再升高, 在盐度为 27 时为最小值为 mg/(g h) 在盐度 32 时, 不同温度的 O: N 范围是 5.525~ 在温度 12 时, 不同盐度下的 O.. N 范围是 4.378~ 不同温度下耗氧率 Q 10 变化范围为 1.054~2.421, 排氨率的 Q 10 变化范围是 0.748~1.256 研究结果为进一步完善大竹蛏的人工养殖技术提供参考关键词 : 大竹蛏 (Solen grandis); 温度 ; 盐度 ; 耗氧率 ; 排氨率中图分类号 : Q142; S968.3 文献标识码 : A 文章编号 : (2017) DOI: /hykx (Solen grandis),,,,,, [1],, 45%, 82.7% [2],, [3], [4-8] [9-11] [12-14] [15-17], [18],, Q 10, [19],, [20-23],,,,,, 1 材料与方法 1.1 大竹蛏和实验用水,,, 1, 3 d,, 12 ±0.3, 1 ph, ph 8.0, 实验方法,, : ; : : ( ); (CARS-48); (2014B11NC092, 2016RQ065) [Foundation: the Cultivation Plan for Youth Agricultural Science and Technology Innovative Talents of Liaoning Province No ; the Grants from the Modern Agro-industry Technology Research System No.CARS-48; the Natural Science Foundation of Dalian No.2014B11NC092, No.2016RQ065] : (1984-),,,, htnie@dlou.edu.cn; (1962-),,,,, yanxiwu@dlou.edu.cn 44 / 2017 / 41 / 4

2 表 1 大竹蛏的生物数据 Tab. 1 Biological characteristics of Solen grandis( X ±SD; n=60) 壳长 (mm) (mm) 湿体质量 (g) 软组织干质量 (g) 壳干质量 (g) 肥满度 (%) 20.90± ± ± ± ± ±0.71 : =( / ) 100%, ( 1) 2, 1, 3 h, 3 (OR, mg/(g h)) (NR, mg/(g h)), ( ),,, 70, 24 h : OR=(D 0 D t ) V/W t OR (mg/(g h)), D 0 D t (mg/l), V (L), W (g), t (h) NR =[(N t N 0 ) V]/W t NR (mg /(g h)), N 0 N t (mg/l) V (L), W (g), t (h) [24] 10/( t t ) 2 1 Q 10 =(M 2 /M 1 ), Q 10, M 1 M 2 (t 1 ) (t 2 ) OR NR O/N=(OR/16) / (NR/14), O/N ; OR [mg/(g h)]; NR [mg /(g h)] [24], 1.3 数据处理 SPSS 19.0 Duncan, P< 结果 2.1 不同温度对大竹蛏耗氧率和排氨率的影响 ~32,, 1.062~1.976 mg/(g h) 17, mg/(g h), (P<0.05) 1 Fig. 1 Effects of temperature on the oxygen consumption rate of the grand jackknife clam Solen grandis , 12~17, mg/(g h), 17~27, mg/(g h), (P<0.05) 2 Fig. 2 Effects of temperature on the ammonia excretion rate of the grand jackknife clam Solen grandis O.. N, O.. N 2,, 5.526~ , Marine Sciences / Vol. 41, No. 4 /

3 表 2 温度对大竹蛏 O.. N 的影响 Tab. 2 Effects of temperature on O.. N ratio of the grand jackknife clam Solen grandis ( ) (mg/(g h)) (mg/(g h)) O N ± ± ± ± ± ± ± ± ± ± 盐度对大竹蛏耗氧率和排氨率的影响 2.2.1, 3,, 22, mg/(g h) 1.071~3.106 mg/(g h) (P>0.05), (P>0.05),, 4 Fig. 4 Effects of salinity on the rate of ammonia of the grand jackknife clam, Solen grandis Fig. 3 3 Effects of salinity on the oxygen consumption rate of the grand jackknife clam Solen grandis O.. N O.. N 3,, O.. N, 4.378~ , 22, , 27 ( 3) 2.3 水温对大竹蛏代谢率强度的影响 2.2.2, 4,, 27, mg/(g h) 0.145~0.176 mg/(g h), 5 Q 10, Q 10 22~ , 12~ Q 10 12~ , 27~ 表 3 盐度对大竹蛏 O.. N 的影响 Tab. 3 Effects of salinity on O.. N ratio of the grand jackknife clam, Solen grandis (mg/(g h)) (mg/(g h)) O N ± ± ± ± ± ± ± ± ± ± 讨论 3.1 温度对大竹蛏耗氧率和排氨率的影响 [22],,,, 46 / 2017 / 41 / 4

4 表 4 水温对大竹蛏代谢率的影响强度 Tab. 4 Extent of temperature influence on the metabolism rate of Solen grandis ( ) Q 10 Q , [25],,,, 32,, 32 17~27, 17, [18] [25],, [21],,, 22, 25 [26], [27] 3 O.. N, O.. N [28] Mayzalld [29],, O.. N 7 Ikeda [30],, O.. N 24 Conover [31],, O.. N 24,, O.. N 5.53~79.04, 32,, Q ~2.5, 2.0 [18], Q ~2.42, 1.43, Q ~1.26, 0.97, ,, 3.2 盐度对大竹蛏耗氧率和排氨率的影响 [32],,,,,, 22, mg/(g h),, 32,,,,,,, 32,,,,,,,, O.. N, [18], O.. N 4.378~16.755, 10.12,, 22, O.. N ,, 22, 17, 27, : [1]. [M]. :. 1998: Qi Zhongyan. Economic mollusca of China [M]. Beijing: China Agriculture Press. 1998, Marine Sciences / Vol. 41, No. 4 /

5 [2]. [J]. ( ), 2002, 7(4): Dai Congjie. Evaluation on Nutrition Components of the Soft Part in Solen gradis [J]. Journal of Jimei University (Natural Science), 2002, 7(4): [3]. [J]., 2002, 9(3): Dai Congjie. The Study on the Compositions of Amino Acids in Soft Part of Solen grandis [J]. Journal of Putian Univeristy, 2002, 9(3): [4],,. [J]., 2009, 31(1): Chen Aihua, Yao Guoxing, Zhang Zhiwei. Studies on artificial propagation of Solen grandis Dunker in pilot scale [J]. Marine Fisheries, 2009, 31(1): [5],,,. [J]., 2015, (4): Song Xianting, Yu Ruihai, Ma Peizhen, et al. A Study on the Artificial Indoor Seedling Raising of Solen grandis [J]. Transactions of Oceanology and Limnology, 2015, (4): [6],,,. [J]., 2012, (8): Wang Xuemei, Lu Yihua, Feng Aixiu, et al. Study on the new mode of healthy seedling cultivation in Solen grandis [J]. Journal of Aquaculture, 2012, (8): [7],,,. [J]., 2004, 21(6): Hou Heyao, Mu Naihai, Song Quanshan, et al. Studies on artificial breeding of Solen grandis [J]. Shandong Fisheries, 2004, 21(6): [8]. [J]., 2015, (5): Yang Hui. Studies on artificial breeding of Solen grandis [J]. Hebei Fisheries, 2015, (5): [9],,,. [J]., 2009, 33(10): Xiao Guoqiang, Chai Xueliang, Shao Yanqing, et al. The reproductive biology of Solen grandis Dunker [J]. Marine Sciences, 2009, 33(10): [10],,,. [J]., 2012, 47(4): Wu Yangping, Chen Aihua, Yao Guoxing, et al. Basic Characteristics of Embryogenesis and Juvenile Development of Solen grandis [J]. Chinese Journal of Zoology, 2012, 47(4): [11],,,. [J]., 2011, 16(2): Li Biquan, Chen Donghui, Zheng Jiemin, et al. The Gonad Observation and the Research on Technique of Artificial Oosperms of Solen grandis Dunker [J]. Journal of Jimei University (Natural Science), 2011, 16(2): [12],,,. [J]., 2004, 21(5): 5-7. Hou Heyao, Wang Junxia, Peng Zuobo, et al. Effects of different salinity on the survival of Solen grandis Dunker [J]. Shandong Fisheries, 2004, 21(5): 5-7. [13],,,. [J]., 2010, 29(5): Chen Aihua, Yao Guoxing, Zhang Zhiwei, et al. Effects of temperature, salinity and sediment on the growth and survival of Solen grandis juveniles [J]. Journal of Tropical Oceanography, 2010, 29(5): [14],,,. [J]., 2012, 40(5): Chen Aihua, Wu Yangping, Yao Guoxing, et al. The composite effects of substrate and temperature on the growth and survival of the Solen grandis [J]. Jiangsu Agricultural Sciences, 2012, 40(5): [15],,,. AFLP [J]., 2011, 39(29): Zhang Tao, Liu Xiangquan, Sun Guohua, et al. The establishment and optimization of AFLP molecular marker reaction system for Solen grandis [J]. Journal of Anhui Agricultural Sciences, 2011, 39(29): [16],,,. (Solen grandis) [J]., 2013, 44(3): Yang Dinglong, Wei Xiumei, Yang Jianmin, et al. Cloning of a ferritin gene from solen grandis and its response to microorganism glycan on transcription level [J]. Oceanologia et Limnologia Sinica, 2013, 44(3): [17] Yuan Y, Li Q, Kong LF, et al. The complete mitochondrial genome of the grand jackknife clam, Solen grandis (Bivalvia: Solenidae): a novel gene order and unusual non-coding region [J]. Molecular Biology Report, 2012, 39: [18] Nie H T, Chen P, Huo Z M, et al. Effects of temperature and salinity on oxygen consumption and ammonia excretion in different colour strains of the Manila clam, Ruditapes philippinarum[j]. Aquaculture Research, 2016, DOI: /are [19],,, [J], 2007, 3(4): Liu Yong, Shi Kuntao, Zhang Shaohua, et al. Advancement of respiratory metabolism study in bivalve mollusus. 48 / 2017 / 41 / 4

6 South China Fisheries Science, 2007, 3(4): [20],, [J], 1997, 21(3): Wang Fang, Dong Shuanglin, Li Deshang, Studies on respiration and excretion of Ruditapes philippinarum and Chlamys farreri [J]. Journal of Fisheries of China, 1997, 21(3): [21],,,. [J], 2007, 37(Sup.): Guo Haiyan, Wang Zhaoping, Yu Ruihai, et al. Effects of temperature and salinity on the oxygen consumption and Ammonia excretion rate of Spisula solidissima [J]. Periodical of Ocean University of China, 2007, 37(Sup.): [22],,, [J], 2007, 14(4): Zhang Yuan, Fang Jianguang, Mao Yuze, et al. Effects of temperature and salinity on oxygen consumption rate and ammonia excretion rate of clam Estellarca olivacea [J]. Journal of Fishery Sciences of China, 2007, 14(4): [23] Tang B, Liu B, Yang H, et al. Oxygen consumption and ammonia-n excretion of Meretrix meretrix in different temperature and salinity [J]. Chinese Journal of Oceanology and Limnology, 2005, 23(4): [24] [S]. GB ,. Code of practice for marine monitoring technology [S]. GB , Standards Press of China [25],,,. [J]., 2009, 37(5): Li Jinbi, Gong Shiyuan, Yu Dahui, et al. Effects of Temperature and Salinity on Rates of Oxygen Consumption and Ammonia Excretion in Pinna pectinata [J]. Journal of Anhui Agriculture Science, 2009, 37(5): [26],,. [J], 2005, 29(8): Chai Xueliang, Fang Jun, Lin Zhihua. Effects of temperature on filtra, oxygtionen consumption and ammonia excretion of Mercenaria mercenaria [J]. Marine Sciences, 2005, 29(8) : [27],,, 3 HSP70 [J], 2015, 39(11): Hao Zhenlin, Liu Jingzhe, Tang Xuejiao, et al. A comparative study of survival, metabolism, immune indi-cators and HSP70 expression in three kinds of shell colors Japanese scallop Mizuhopecten yessoensis under high tem-perature stress [J]. Marine Sciences, 2015, 39(11): [28],,, [J], 2013, 28(2): Hao Zhenlin, Ding Jun, Fen Yue, et al. Effect of high temperature on survival, oxygen consumption and ammonia-n excretion in yesso scallop Mizuhopecten yessoensis [J]. Journal of Dalian Fisheries University, 2013, 28(2): [29] Mayzalld P. Respiration and nitrogen excretion of zooplankton: IV. The influence of starvation on the metabolism and biochemical composition of some species [J]. Marine Biology, 1976, 37(1): [30] Ikeda T. Nutrition ecology of marine zooplankton [J]. Memoirs of the Faculty of Fisheries Hokkaido University, 1974, 22(1): [31] Conover R J, Corner E D S. Respiration and nitrogen excretion by some marine zooplankton in relation to their life cycles [J]. Journal of the Marine Biological Association of the United Kingdom, 1968, 48: [32] Wilbur A E. Physiological energetics of the ribbed mussel Geukensia demissa (Dillwyn) in response to increased temperature[j]. Journal of Experimental Marine Biology and Ecology, 1989, 131: Marine Sciences / Vol. 41, No. 4 /

7 Studies on the effect of temperature and salinity on oxygen consumption and ammonia excretion in the grand jackknife clam Solen grandis NIE Hong-tao 1, 2, JIANG Li-wen 1, 2, WANG Hai-lun 1, XING Ning-ning 1, YANG Feng 1, 2, YAN Xi-wu 1, 2 (1. Engineering and Technology Research Center of Shellfish Breeding of Liaoning Province, Dalian Ocean University, Dalian , China; 2. College of Fisheries and Life Science, Dalian Ocean University, Dalian , China) Received: Aug.17, 2016 Key words: Solen grandis; Temperature; Salinity; Oxygen consumption; Ammonia excretion Abstract: Temperature and salinity are the main factors affecting the metabolism of aquatic biological organisms, and these environmental factors vary seasonally. This study investigated the effects of temperature and salinity on oxygen consumption and ammonia excretion of the grand jackknife clam Solen grandis. The experimental setup included a temperature gradient (12, 17, 22, 27, and 32 ) and a salinity gradient (12, 17, 22, 27, and 32). The results demonstrate that temperature has a significant effect on oxygen consumption and ammonia excretion rates in this strain (P < 0.05). As the temperature increased, oxygen consumption and ammonia excretion first decreased and then increased. The lowest oxygen consumption rate is mg/(g h) at 32, and the lowest ammonia excretion rate is mg/(g h) at 17. As salinity increased, oxygen consumption first increased and then decreased, whereas ammonia excretion first decreased and then increased. The highest oxygen consumption rate is mg/(g h) for a salinity value of 22, and the lowest ammonia excretion rate is mg/(g h) for a salinity value of 27. At 12, the O.. N ratio ranged from to as the salinity value increased from 12 to 32. At a salinity value of 32, the O.. N ratio ranged from to as the temperature increased from 12 to 32. The information regarding its response to different temperature and salinity values has implications in the S. grandis aquaculture industry. ( 本文编辑 : 康亦兼 ) 50 / 2017 / 41 / 4

Microsoft Word - 22 栗志民.doc

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