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1 6 11 Vol. 6 No Journal of Food Safety and Quality Nov., 2015 杨海燕, 吴文龙 *, 李维林, 姚蓓, 卫云丽, 闾连飞 (, ) 摘要 : 目的 (Climax), (methyle jasmonate, MeJA) 方法 (0, 1, 10, 100 µmol/l)meja h, 4, 14 d 结果 MeJA,, (superoxide dismutas, SOD) (peroxidase, POD), O 2 ( ) (malondialdehyde, MDA) 结论, 10 µmol/l MeJA 关键词 : ; ; Effect of methyle jasmonate treatments on physioloical changes of YANG Hai-Yan, WU Wen-Long *, LI Wei-Lin, YAO Bei, WEI Yun-Li, LV Lian-Fei (Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing , China) ABSTRACT: Objective To investigate the effect of methyle jasmonate (MeJA) treatment on the physiological changes of. Methods The variety Climax was fumigated with 0, 1, 10, 100 µmol/l Me- JA for 10 h and stored at 4, and then changes of quality and antioxidant ability were measured every 14 days during fruit storage. Results The results showed that different concentrations of MeJA treatment could significantly inhibit decay of, delay the decline of firmness and the soluble solids. It also increased the activities of superoxide dismutas and peroxidase, enhanced the fruit resistance. Besides, it induced the generation of O 2 and malondialdehyde. Conclusion 10 µmol/l MeJA treatment has a certain application value in the storage of blueberry. KEY WORDS: blueberry; methyle jasmonate; fresh-keeping 1 引言 (Vaccinium spp.), (Ericaceae) [1],,,, [2] 6~8,,,, 20~30 基金项目 : (CX(15) ) (BE ) Fund: Support by Jiangsu Agricultural Science and Technology Innovation Fund Project (CX(15)1026 Jiang ) and Jiangsu Science and Technology Support Project (BE ) * 通讯作者 :,, wwl@163.com *Corresponding author: WU Wen-Long, Professor, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Qianhu Houcun, Nanjing, , China wwl@163.com

2 ~7 d, [3],, (methyle jasmonate, MeJA),,, [4], MeJA,,,, [5,6], MeJA (Climax), MeJA, MeJA, MeJA 2 材料与方法 2.1 实验材料,,, 8~9, 2.2 处理方法 4, 2.0 kg,, 20, µmol/l MeJA 10 h,, 1 h,, 200 g, 4, 90%, 28 d, 14 d, 2.3 测定方法果实硬度的测定 FT-02 ( EFFEGI) 果实腐烂率的测定 : (%)=( / ) 果实可溶性固形物的测定 PAL-1 ( ATAGO) 果实超氧化物歧化酶 (superoxide dismutas, SOD) 活性的测定 Beyer [7], 200 g, 4.0 g, 30 ml 50 mmol/l (ph 7.8), g 10 min,, ml SOD 50% SOD (U) 果实过氧化物酶 (peroxidase, POD) 活性的测定 [8], 300 µl, 3 ml 470 nm, 30 s, 果实超氧阴离子自由基 (O 2 ) 产生速率的测定 [9] 0.5 ml 0.5 ml 50 mmol/l (ph 7.8) 1 ml 1 mmol/l, 25 1 h, 1 ml 17 mmol/l 1 ml 7 mmol/l α-, min, 530 nm, O 果实丙二醛 (malondialdehyde, MDA) 含量的测定 Health [10] 200 g, 4.0 g, 30 ml 10%, 4000 g 10 min, 2 ml, 2 ml 0.6% (TBA), 30 min,,, 532 nm 600 nm 450 nm, MDA 统计分析 Excel 2003, Duncan (P<0.05) 3 结果与分析 3.1 蓝莓果实硬度的变化,,, 28 d 100 µmol/l MeJA, MeJA, 10 µmol/l MeJA, 28 d (P 0.05),

11, : 4485 3.3 蓝莓果实可溶性固形物含量的变化 3,, 14 d, MeJA, 28 d, MeJA, 10 µmol/l MeJA,,, (P 0.

2 蓝莓果实腐烂率的变化 2,, 14 d,, 10 µmol/l MeJA, 61.2%; 28 d,,,, 10 µmol/l MeJA, 36.2%, (P 0.

4 蓝莓果实 SOD 活性的变化 SOD Mehler O 2, O 2, H 2 O 2 4,, SOD MeJA, SOD, 14 d, 10 µmol/l MeJA (P 0.

3 11, : 蓝莓果实可溶性固形物含量的变化 3,, 14 d, MeJA, 28 d, MeJA, 10 µmol/l MeJA,,, (P 0.05) MeJA 1 MeJA Fig. 1 Effect of MeJA treatment on the firmness of 3.2 蓝莓果实腐烂率的变化 2,, 14 d,, 10 µmol/l MeJA, 61.2%; 28 d,,,, 10 µmol/l MeJA, 36.2%, (P 0.05) 3 MeJA Fig. 3 Effect of MeJA treatment on the soluble solid content of 3.4 蓝莓果实 SOD 活性的变化 SOD Mehler O 2, O 2, H 2 O 2 4,, SOD MeJA, SOD, 14 d, 10 µmol/l MeJA (P 0.05), 42.1% 3.5 蓝莓果实 POD 活性的变化 2 MeJA Fig. 2 Effect of MeJA treatment on the decay rate of (POD),, POD, 5, POD, MeJA POD, 10 µmol/l MeJA 14 d (P 0.05), 10.8% 28 d, 1 µmol/l

4486 6 100 µmol/l MeJA POD, 57.7%(P 0.05) 1 µmol/l MeJA (P 0.05) MeJA, 4 MeJA SOD Fig.

6 Effect of MeJA treatment on the generation rate of O 2 of 3.

05) MeJA, 5 MeJA POD Fig. 5 Effect of MeJA treatment on the POD activity of 3.

4 µmol/l MeJA POD, 57.7%(P 0.05) 1 µmol/l MeJA (P 0.05) MeJA, 4 MeJA SOD Fig. 4 Effect of MeJA treatment on the SOD activity of 6 MeJA O 2 Fig. 6 Effect of MeJA treatment on the generation rate of O 2 of 3.7 蓝莓果实 MDA 含量的变化 MDA,, 7,, MDA, MeJA MDA, 14 d 10 µmol/l MeJA, 28 d 1 µmol/l MeJA (P 0.05) MeJA, 5 MeJA POD Fig. 5 Effect of MeJA treatment on the POD activity of 3.6 蓝莓果实超氧阴离子 (O 2 ) 产生速率的变化 O 2, 6,, O 2, 14 d, 174.7%,, O 2 MeJA O 2, 14 d 10 µmol/l MeJA, 28 d 7 MeJA MDA Fig. 7 Effect of MeJA treatment on the MDA content of

5 11, : 讨论,,, MeJA,,, MeJA,, MeJA [5,6,11], µmol/l MeJA,,, 10 µmol/l MeJA,,,,,, MDA,, [11-13] SOD POD SOD O 2, H 2 O 2, H 2 O 2 POD H 2 O, [12],, SOD POD, 1 10 µmol/l MeJA SOD, µmol/l MeJA POD, MeJA,, MeJA O 2 MDA [14] [15] MeJA,,, MeJA,,, 5 结论, 4, MeJA, SOD POD,,,, 10 µmol/l MeJA, MeJA,, MeJA O 2 MDA, 参考文献 [1] Angeletti P, Castagnasso H, Miceli E, et al. Effect of preharvest calcium applications on postharvest quality, softening and cell wall degradation of two blueberry (Vaccinium corymbosum) varieties [J]. Postharvest Bio Tec, 2010, 58(2): [2] Naczk M, Grant S, Zadernowski R, et al. Protein precipitating capacity of phenolics of wild blueberry leaves and fruits [J]. Food Chem, 2006, 96(4): [3],,,. [J]., 2013, 34(14): Liu M, Fan XG, Wang ML et al. Influence of different packaging methods on physiological properties of blueberry during postharvest storage [J]. Food Sci, 2013, 34(14): [4] Arimura G, Ozawa R, Shimoda T, et al. Herbivory-induced volatiles elicit defence genes in lima bean leaves [J]. Nature, 2000, 406(6795): [5],,. [J]., 2012, 33(10): Zhang HY, Yin JY, Wan SB. Effects of salicylic acid and methyl jasmonate in combination with low temperature conditioning on chilling injury in honey peach [J]. Food Ind. 2012, 33(10): [6],,,. [J]., 2013, 34(6): Wang KT, Zheng YH, Tang WC, et al. Effect of methyl jasmonate treatment on synthesis and antioxidant of phenolic acids in grape berries and corresponding mechanisms [J]. Food Sci, 2013, 34(6): [7] Beyer WF, Fridsovich I. Assaying for superoxide dismutase activity: Some large consequences of changes in conditions [J]. Anal Biochem, 1987, 161(2):

4488 6 [8] Maehly AC. Plant peroxidase [J]. Method Enzymol, 1955, (2): 801 813. [9],. [J], 1990, (6):94 95 Luo GH, Wang AG.

6 [8] Maehly AC. Plant peroxidase [J]. Method Enzymol, 1955, (2): [9],. [J], 1990, (6):94 95 Luo GH, Wang AG. The quantitative relationship between the generation rate of O 2 and the reaction of hydroxylamine [J]. Plant Physiol Commun, 1990, (6): [10] Heath R L, Parker L. Photoperoxidation in isolated chloroplasts I. Kinetics and stoichiometry of fatty acid peroxidation [J]. Arch Biochem Biophys, 1968, 125(1): [11],,,. [J]., 2013, 34(4): Hu WZ, Jiang AL, Cai H, et al. Effect of MeJA treatments on physiological and biochemical changes for Actinidia arguta [J]. Sci Tech Food Ind, 2013, 34(4): [12],,,. [J]., 2013, 27(5): Wang YJ, Ma C, Zhang W, et al. Effects of silicon on strawberry growth and physiological characteristics under drought stress [J]. Acta Agr Nucl Sin, 2013, 27(5): [13],,,. MeJA [J]., 2012, 39(2): Jin P, Lv MW, Sun CC, et al. Effects of methyl jasmonate in combination with low temperature conditioning on chilling injury and active oxygen metabolism in loquat fruits [J]. Acta Hortic Sin. 2012, 39(2): [14],. [J]., 2006, 33(2): Han J, Tian SP. Effects of exogenous methyl jasmonate on chilling injury and physiology and biochemistry in postharvest cucumber [J]. Acta Hortic Sin. 2006, 33(2): [15],. [J]., 2007, 16(3): Wu GZ, Zeng RS. The influence of protective enzymes of perpendicular Gaozhou wild rice (Oryza rufipogon) treated with exterior signal compounds salicylates and jasmonates [J]. Acta Agric Boreali-occidentalis Sin, 2007, 16(3): 作者简介 ( 责任编辑 : 金延秋 ) 杨海燕, 博士, 助理研究员, 主要研究方向为小浆果类生物技术研究 haiyanyang_025@126.com 吴文龙, 学士, 研究员, 主要研究方向为黑莓和蓝莓引种育种与加工利用技术研究 wwl@163.com

标题

648 江苏农业学报 (Jiangsu J. of Agr. Sci. ),2013,29(3):648 ~ 653 h ttp: / / w w w. jsn y x b.c o m 王摇丹, 孙摇蕾, 孙家正, 等. 桑葚冻结过程中的温度变化特性 [J]. 江苏农业学报,2013,29(3):648 鄄 653. doi:10. 3969