Microsoft Word - 5——祝斐_news_.doc

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1 光周期对松江鲈鱼生长和性腺发育影响的初步研究祝斐, 张志勇, 徐献明, 张志伟, 陈淑吟, 贾超峰 (, ) 摘要 : 在松江鲈鱼 (Trachidermis fasciatus) 生长和性腺发育的关键时期, 挑选其同规格的幼苗分为长光照组 (LL) 与自然光照组 ( 对照组, NL), 研究光周期对其生长和性腺发育的影响本研究将 LL 组与 NL 组分别设计 3 组平行, 共计 150 尾实验起始 : LL 组体质量 : (3.7256±0.8236)g, 全长 : (6.2253±0.3687)cm; NL 组体质量 : (3.7179±0.7893)g, 全长 : (6.2053±0.3436)cm, 组间体质量及全长差异不显著经过 76 d 的光周期实验, 在前中期体质量检测中, LL 组体质量全长大于 NL 组 ; 最终 LL 组体质量 : ( ± ) g, 全长 : ( ±0.3861) cm; NL 组体质量 : ( ±2.4460) g, 全长 : ( ±0.6432)cm; LL 组的肝指数均值为 (9.1525±0.5053)%, 相对于 NL 组的 (5.2675±0.1504)%, 为极显著 (P<0.001) 最终, LL 组的性腺发育指数均值为 (5.8150±0.1396)%, 相对于 NL 组的 (5.7625±0.3404)%, 为极显著 (p<0.001) LL 组肝指数 (HSI) 性腺发育指数(GSI) 均显著高于 NL 组 ; 卵巢从外观及卵径判断, LL 组已发育至第 V 期, 大部分卵粒饱满, 卵粒清亮饱满相互黏连, 而 NL 组卵巢为第 IV 期, 颜色偏红且卵径显著偏小 LL 组卵径均值为 (1.2118±0.3136)mm, 而 NL 组则为 (0.8356±0.0935)mm, LL 组卵径偏大 (P<0.001) 结果表明: 在松江鲈鱼的幼鱼至成鱼阶段, 即生殖洄游阶段, 适当的延长光照对松江鲈鱼的性腺发育及肝脏能量储均存有显著性影响关键词 : 松江鲈鱼 (Trachidermis fasciatus); 光周期 ; 性腺发育中图分类号 : S917 文献标识码 : A 文章编号 : (2016) doi: //hykx (Trachidermis fasciatus),,, 鲉 (Scorpaeniforme) (Cottidae) (Trachidermis), [1],, 11 ( ) 9~12 [2], 10,,, [3],,, (BW) (HSI) (GSI),,, 1 材料与方法 1.1 材料 ( ), ( ), 2, (Neomysis awatschensis),, 6, 3 : ; : : (D ) [Foundation: The Key Programs of Three Reforms in Aquaculture Engineering of Jiangsu Province(D )] : (1987-),,,,, : , ebancool@126.com;,, : , @139.com 32 / 2016 / 40 / 9

2 (LL 1, LL 2, LL 3 ) 3 (NL 1, NL 2, NL 3 ), 25 ; 1 m 0.5 m 0.8 m 1.2 方法, 2.7 m 4.6 m 1 m 6 1, 3, 25 : I ( ), LL 1 LL 2 LL 3 85 w,, NL 1 NL 2 NL 3 ( ), 70%,,, 14 h.. 0 h( ), - (H.E), 2350, V9.0 (NL)GSI HSI t 2 结果 2.1 光照对松江鲈鱼成活率的影响 LL : (3.7256±0.8236)g, : (6.2253±0.3687)cm; NL : (3.7179± )g, : (6.2053±0.3436)cm, P=0.478( ), P=0.732( ) 76 d,, LL NL, ; LL : ( ± )g, : ( ±0.3861)cm; NL : ( ± )g, : ( ±0.6432)cm, (P=0.754);, 1 LL NL 1.3 养殖管理 : ; (Gambusia affinis) ;,,,, 1.4 测定指标, 6 ; 10 15,, 5 HSI(%) GSI(%) HSI(%)= / 100% GSI(%)= / 100%, LL NL,,,, LL NL 90, 1.5 数据统计 ±, SPSS16.0, Origin7.5,, (One way ANOVA), S-N-K, P<0.05 (LL) 1 LL NL Fig. 1 Experimental photoperiods of long time light (LL) group and natural light (NL) group 2.2 光照对松江鲈鱼体质量的影响 9 30 ( 2) ~11 14 LL NL, (P<0.001), (P=0.093), (P=0.754), LL NL,, 2.3 肝指数和性腺发育指数的变化规律及差异, Marine Sciences / Vol. 40, No. 9 /

,,, V 2 LL, NL Fig. 2 Curves for body weight of long time light (LL) group and natural light (NL) group n.s. P>0.05, **. P<0.01( 3, 4 ) n.s. not significant, **. significant, P<0.01 (same as Fig.

3 ,,, V 2 LL, NL Fig. 2 Curves for body weight of long time light (LL) group and natural light (NL) group n.s. P>0.05, **. P<0.01( 3, 4 ) n.s. not significant, **. significant, P<0.01 (same as Fig. 3 and Fig. 4), , ( 3) ( 4) 5 (HSI) (GSI) t, 5 LL NL HSI,, P=0.032 P=0.000, 3, LL, ; ~10 30, 76 d, LL (9.1525±0.5053)%, NL (5.2675±0.1504)%, (P<0.001), (5.8150±0.1396)%, NL (5.7625±0.3404)%, (P<0.001) [4], [5],, NL, IV,,,, LL, 3 (HSI) Fig. 3 The dynamic alterations of HSI for long time light (LL) group and natural light (NL) group 4 (GSI) Fig. 4 The dynamic alterations of GSI for long time light (LL) group and natural light (NL) group, 5A 5B 6,, 76 d, LL (1.2118±0.3136)mm, NL (0.8356±0.0935) mm, LL (P=0.000) 1.2 mm 1.2 mm 2, Fig. 5 5 (A) (B) Comparison of egg size between LL group and NL group (A) and a section of ovary of LL group and NL group (B) 34 / 2016 / 40 / 9

4 Fig. 6 6 LL NL Comparison of egg size between LL group and NL group NL 1.2 mm 2.4 塑料大棚内的温度变化规律, ( 7) 10,,,,, 7, Fig. 7 The dynamic alterations of water temperature and air temperature 3 讨论 3.1 光周期对松江鲈鱼生长的影响 [6],,, (Perca flavescens) [7] (Salmo salar) [8],,, ~11 14, (P<0.001), ~12 14, : (1), [9], (Esox lucius) [10] (Acipenser gueldenstaedti) [11] [12] (Oplegnathus fasciatus) [13] (Salvelinus pluvius) [14] [15] (Salvelinus alpinus),,, ; (2) 2 3 4, : , LL NL,, [2], [16],,,, 12,,,,,,, 3.2 光周期对松江鲈鱼肝指数的影响 Marine Sciences / Vol. 40, No. 9 /

5 ,,, [17] [18], FSSP( ),,,,,, [5], HSI, ; [19], 3.3 光周期对松江鲈鱼卵巢的影响,, BPG,, (Scophthalmus maximus) [20] (Salmo salar) [8] (Sciaenops ocellatus) [21] (Verasper variegates) [22], 1.48~2.21 mm [23], 23% 1.48 mm;,, 14 h,,,, [21], [5] 14 h,,,,,, : [1],,. [J]., 1996, 11: 1-3. Zhou Ming, Wei Zhengdao, Chen Haiming. Preliminary study of the disgestive system of newborn roughskin seulpin, Trachidermis fasciatus Heckel using histological techniques[j]. Modern Fisheries Information, 1996, 11: 1-3. [2],,,. [J]., 2013, 7: Xu Xianming, Zhang Zhiyong, Han Xiaolei, et al. Study on growth characterization of Trachidermus fasciatus farmed [J]. Journal of Aquaculture, 2013, 7: [3],. (Trachidermus fasciatus) [J]. ( ), 1997, 36(5): Wei Zhengdao, Wang Changxie.Environmental factors controlling the growth of Trachidermus fasciatus[j]. Journal of Fudan University, 1997, 36(5): [4],,,. [J]., 1983, 7(1): Liu Jun, Liu Guoan, Chen Shuqun, et al. Studys on the gonadal development of Tilapia nilotica[j]. Acta Hydrobiologica Sinica, 1983, 7(1): [5],,. [J]., 2013, 1: Zhao Yijie, Zhang Meizhao, Wen Haishen. Histological observation of gonad development in trachidermus fasciatus[j].transactions of Oceanology and Limnology, 2013, 1: [6],. LED [J]., 2003, 14(3): Yang Shiguang, Li Xueyong. The action and influence of the color light of LEDs during on the growth of water creature[j].journal of Lighting Engineering, 2003, 14(3): [7]. [J]., 2006, 15(3): Chen Wenyin. Effects of photoperiod on the growth of yellow perch Perca flavescens[j]. Journal of Shanghai Fisheries University, 2006, 15(3): [8] Hansen T, Karlsen Ø, Taranger G L, et al. Growth, go- 36 / 2016 / 40 / 9

6 nadal development and spawning time of Atlantic cod (Gadus morhua) reared under different photoperiods[j]. Aquaculture, 2001, 203(1): [9] Smith H T, Schreck C B, Maughan O E. Effect of population density and feeding rate on the fathead minnow (Pimephales promelas)[j]. Journal of Fish Biology, 1978, 12(5): [10],,. [J]., 2006, 30(1): Huang Ningyu, Xia Lianjun, Yao Zongli, et al. The influence of stocking density and water temperature on growth in juvenile Esox lucius raised in greenhouse[j]. Journal of Fisheries of China, 2006, 30(1): [11],,,. [J]., 2014, 38(6): Song Zhifei, Wen Hanshen, Li Jifang et al. The influence of stocking density on the growth performance of juvenile Russian sturgeon( Acipenser gueldenstaedti) in flowing water cultivation[j]. Journal of Fisheries of China, 2014, 38(6): [12],,,. [J]., 2007, 4: Liu Wenkui, Fan Qixue, Du Haiming, et al. Effect of stocking density on growth and survival of larval hybrid snakehead[j]. Freshwater Fisheries, 2007, 4: [13],,,. [J]., 2012, 21(4): Liu Minhai, Peng Zhilan, Zhang Fengping, et al. Effect of stocking density on the growth, feeding and behavior of Oplegnathus fasciatus[j]. Journal of Shanghai Ocean University, 2012, 21(4): [14],,,. [J]., 2009, 6: Zhang Yongquan, Yin Jiasheng, Wang Bingqian, et al. Effects of stocking density on growth, survival and behavior of juvenile Siberian charr Salvelinus leucomaenis[j]. Journal of Dalian Fisheries University, 2009, 6: [15] Brown G E, Brown J A, Srivastava R K. The effect of stocking density on the behaviour of Arctic charr (Salvelinus alpinus)[j]. Journal of Fish Biology, 1992, 41(6): [16],,,. [J]., 2002, 13(6): Zhuang Ping, Li Dapeng, Wang Xueming, et al Effect of stocking density on growth of juvenile Acipenser schrenckii.[j]. Chinese Journal of Applied Ecology, 2002, 13(6): [17]. [J]., 1984, 5: Zhu Chengde. The relationship between big Salangid liver index and ovarian development[j]. Freshwater Fisheries, 1984, 5: [18],. [J]., 1999, 20(6): Hou Yayi, Suzuki Yuzuru. Immune function of immature rainbow trout (Oncorhynchus mykiss) related with photoperiod[j]. Zoological Research, 1999, 20(6): [19],,,. [J]., 2010, 34(11): 1-5. Chen Hui, Lin Guowen Liu Zhaokun, et al. Study on growth characters of cultured Pseudosciaena crocea originated from Eastern Fujian[J]. Marine Sciences, 2010, 34(11): 1-5. [20] Imsland A K, Folkvord A, Jónsdóttir Ó D, et al. Effects of exposure to extended photoperiods during the first winter on long-term growth and age at first maturity in turbot ( Scophthalmus maximus)[j]. Aquaculture, 1997, 159(1): [21],. [J]., 1999, 23(3): Liu Hongjie, Mao Xinghua. Reproductive behavior and technology of inducing spawning of scuaebops ocellatus[j]. Marine Sciences, 1999, 23(3): [22],,,. [J]., 2011, 18(4): Xu Yongjiang, Liu Xuezhou, Wang Qingyin, et al. Annual gonadal maturation cycle of captive spotted halibut, Verasper variegatus: correlation with serum sex steroids and photothermal regulation[j]. Journal of Fishery Sciences of China, 2011, 18(4): [23] 堃,,. [J]. ( ), 1984, 23(1): Li Kunbao, Shao Bingxu, Rui Jusheng.A histological study on the digestive organs of trachidermus fasciatus during seaward migration[j]. Journal of Fudan University, 1984, 23(1): Marine Sciences / Vol. 40, No. 9 /

7 Preliminary study on growth and gonadal development of reared under different photoperiods ZHU Fei, ZHANG Zhi-yong, XU Xian-ming, ZHANG Zhi-wei, CHEN Shu-yin, JIA Chao-feng (Institute of Oceanology & Marine Fisheries, Nantong , China) Received: Jun. 6, 2015 Key words: Trachidermis fasciatus; photoperiods; gonadal development Abstract: This study investigated the effect of different photoperiods on body weight, growth, and gonadal development of the fish Trachidermis fasciatus. A total of 150 fish were divided into two parallel groups; one group was exposed to natural light (NL) and the other was exposed to long time light (LL). After 76 days of exposure to photoperiod, the fish in the LL group exhibited better changes than the NL group of fish, which were later influenced by population density, although the differences were not significant. The HSI was (9.1525±0.5053)% in the LL group, while it was (5.2675±0.1504)% in the NL group; the GSI was (5.8150±0.1396)% in the LL group, and it was (5.7625±0.3404)% in the NL group. These results indicated that both HSI and GSI were significantly higher in the LL group than in the NL group. At the end of the study, the ovary in the LL group exhibited initial stage V development and most of the egg grains were full, showing clear and complete mutual adhesion. However, in the NL group, the ovary was red and apparently smaller. Egg diameters were (1.2118±0.3136)mm in the LL group and (0.8356±0.0935)% in the NL group. ( 本文编辑 : 谭雪静 ) 38 / 2016 / 40 / 9

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