Microsoft Word 王建明-二校 677、678

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1 7 2 Vol. 7 No Journal of Food Safety and Quality Feb., 王建明 1, 燕瑾 2, 孙红 3, 李洁 3, 张金秀 3, 王立安 (1., 67000; 2., ; 3., ) 3* 摘要 : 目的 (Hygrophorus lucorum) (Helvella elastica) (Lactarius pubescens) (Coprinus micaceus)4 方法 4, DPPH Fe 2+, 结果, 4 DPPH, 10 mg/ml, DPPH 85% 0.5 mg/ml, BHA 4, 结论 ;, 关键词 : ; ; DPPH ; ; Fe 2+ Research on in vitro antioxidant activity of ethanol extracts from 4 kinds of wild fungi WANG Jian-Ming 1, YAN-Jin 2, SUN Hong 3, LI Jie 3, ZHANG Jin-Xiu 3, WANG Li-An 3* (1. Hebei Normal University for Nationalities, Chengde , China; 2. The Radiation Research Center of Beijing, Beijing , China; 3. College of Life Science, Hebei Normal University, Shijiazhuang , China) ABSTRACT: Objective To investigate the in vitro antioxidant activity of Hygrophorus lucorum, Helvella elastica, Lactarius pubescens and Coprinus micaceus. Methods Based on the determination of total phenol, total flavonoids and crude terpene of four kinds of mushroom ethanol extracts, DPPH radical scavenging experiment, mice liver tissue in vitro spontaneous lipid peroxidation experiment and Fe 2+ chelating experiment were carried out to detect the antioxidant activity. Results The antioxidant activity of Hygrophorus lucorum, Helvella elastica, Lactarius pubescens were better than that of Coprinus micaceus. Four kinds of mushroom ethanol extracts had certain antioxidant activity against DPPH. The radical-scavenging ability was more than 85% when the mushroom ethanol extract reached to 10 mg/ml except for the Coprinus micaceus. The 基金项目 : ( D) ( D) Fund: Supported by the Science and Technology Research and Development Projects Hebei Province ( D) and the Earmarked Fund for Hebei Edible Fungi Innovation Team of Modern Agro-industry Technology Research System ( D) * 通讯作者 :,, wlian1965@126.com *Corresponding author: WANG Li-An, Professor, College of Life Science, Hebei Normal University, Shijiazhuang , China. wlian1965@126.com

2 2, : inhibition of lipid peroxidation activity was higher than that of the positive control BHA when the concentration of Hygrophorus lucorum was more than 0.5 mg/ml. Fe 2+ chelating experiment showed that the four kinds of mushroom ethanol extracts all had the ability of chelating Fe 2+. Conclusion The antioxidant activity of fungi is related to total phenolics and total flavonoids. Hygrophorus lucorum, Helvella elastica, Lactarius pubescens have good antioxidant activity, which will provide a theoretical basis for the rational development of large fungi. KEY WORDS: fungi; ethanol extract; DPPH free radical; spontaneous lipid peroxidation; Fe 2+ chelation 1 引言 (reactive oxygen species, ROS),,,, : [1,2],,,,,,, [3,4] Soares [5] (Agaricus brasiliensis) 6 Liang [6] (Phellinus linteus), Sarikurkcu [7] (Lactarius sanguifluus) (Suillus bovines) (Boletus edulis) (Xerocomus chrysenteron), 4 [8], 18, (Hygrophorus lucorum) (Helvella elastica) (Lactarius pubescens) (Coprinus micaceus)4,,, 4,, 2 材料与方法 2.1 材料 4 40, 800 g 95% ; Na 2 CO 3 EDTA FeCl 2 Folin- (FerroZine) ( ) ; 1, (DPPH) (BHA) Sigma (JA2003N, ); (RE-5203, ); (DZF-6020, ); (MIKRO-22R, Hettich ); (FW177, ); 756MC ( ) 种野生菌乙醇提取物的制备 4, 95% 3,,,, 4

3 乙醇提取物中总酚含量的测定标准曲线 [9], 0.1 mg/ml, μg/ml, 1 ml Folin-, 3~4 min 10% Na 2 CO 3 1 ml, 10 ml 90 min, 760 nm, : Y=0.0097X+0.114, r 2 =0.9956(n=11) 总酚含量的测定, 1 mg/ml 1 ml, 760 nm OD, 2.4 乙醇提取物中总黄酮含量的测定标准曲线 0.1 mg/ml [10], ml, 1 ml, 10% KOH 0.5 ml, 5 min, 10 ml, 415 nm, : Y=3.189X , r 2 =0.9991(n=5) 总黄酮含量的测定, 1 mg/ml 1 ml, 415 nm OD, 2.5 各提取物中粗三萜含量的测定标准曲线 [11], 0.2 mg/ml ml, 5% 0.4 ml 1.6 ml,, min,, 10 ml 560 nm, : Y=8.04X , r 2 =0.9991(n=5) 粗三萜含量的测定 1 mg/ml 0.5 ml,, 560 nm,, : (%)=m/c V 100% : m (mg); c (1 mg/ml); V (ml) 2.6 各提取物清除 DPPH 实验, BHA, 1 ml DPPH, 517 nm, DPPH [12,13] 清除率 (% ) [ A0 ( Ai Aj) ]/ A0 100 % A 0 2 ml DPPH +2 ml OD ; A i 2 ml DPPH +2 ml OD ; A j 2 ml +2 ml OD 2.7 各提取物抑制脂质过氧化活性测定 BHA, 1 ml [14], 532 nm, : 抑制率 (%) [ A0 ( A Ab) ] / A0 100% : A 0 ; A ; A b 2.8 各提取物金属离子螯合能力测定 EDTA, 1 ml EDTA, 0.1 ml 2 mmol/l FeCl ml,, 0.1 ml 5 mmol/l Ferrozine,, 10 min, 562 nm, 3 [15] : 抑制率 % [ A0 A1 / A0] 100% : A 0 ; A 1 3 结果与分析种野生菌乙醇提取物中总酚总黄酮粗三萜含量的比较 4 1 1, 4, 100

4 2, : mg mg;, 1.41 mg, 100 mg mg,, mg;, 100 mg mg,, mg;, 3.46 mg 种野生菌乙醇提取物对 DPPH 自由基的清除作用 4 DPPH 2 2, 4 DPPH, BHA, DPPH : BHA> > > >, IC mg/ml 6 mg/ml, DPPH 85% ; 8 mg/ml, DPPH 96%, ;,, 种野生菌乙醇提取物对小鼠肝脂质过氧化的抑制 4 3 3, 4, BHA, IC 50, : BHA> > > > 0.5 mg/ml, BHA; 10 mg/ml BHA; 4 mg/ml, 88% ;, 10 mg/ml, 80% 种野生菌乙醇提取物对 Fe 2+ 的螯合能力 4 4 4, 4, EDTA, EC50, : EDTA> > > >, 4 mg/ml, 70%,, ; 10 mg/ml, 70% ;, 10 mg/ml, 50% 表 1 4 种野生菌乙醇提取物中总酚总黄酮粗三萜含量 (n=3) Table 1 Total phenol, total flavonoids and crude terpene of four kinds of mushroom ethanol extracts (n=3) (mg/100 mg) 10.14± ± ± ± ± ± ± ± ± ± 表 2 4 种野生菌乙醇提取物对 DPPH 清除率 (%)(n=3) Table 2 DPPH free radical scavenging activity of four kinds of mushroom ethanol extracts (%) (n=3) (mg/ml) BHA ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ±0.020 IC

5 680 7 Table3 表 3 4 种野生菌乙醇提取物对小鼠肝脂质过氧化的抑制率 (%)(n=3) The lipid peroxidation inhibition effect of four kinds of mushroom ethanol extracts in mice liver (%) (n=3) (mg/ml) BHA ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ±0.051 IC 表 4 4 种野生菌乙醇提取物对 Fe 2+ 的螯合能力 (%)(n=3) Table 4 Fe 2+ chelation activities of mushroom ethanol extracts(%) (n=3) (mg/ml) EDTA ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ±0.007 EC 讨论,,, 4,, 3,, 4 ; 4,,,,, 3 4 3, DPPH 3 ; Fe 2+,,,,,, [16],,,, 5 结论 4

2, : 4 681 ;, 参考文献 [1] Tajalli F, Malekzadeh K, Soltanian H, et al. Antioxidant capacity of several Iranian, wild and cultivated strains of the button mushroom [J].

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6 2, : ;, 参考文献 [1] Tajalli F, Malekzadeh K, Soltanian H, et al. Antioxidant capacity of several Iranian, wild and cultivated strains of the button mushroom [J]. Braz J Microbiol, 2015, 46(3): [2] Steenvoorden DP1, van Henegouwen GM. The use of endogenous antioxidants to improve photoprotection [J]. J Photochem Photobiol B, 1997, 41(1 2): [3],,. [J]., 2014, 42(29): Ding JH, Zhang JF, Ding FJ. Research progress on active antioxidant components from endophytic fungi [J]. J Anhui Agric Sci, 2014, 42(29): [4],,,. [J]., 2008, 29(9): Hou J, Liu F, Li L, et al. Research progress on antioxidant components from fungi [J]. Food Sci, 2008, 29(9): [5] Soares AA, de Souza CGM, Daniel FM, et al. Antioxidant activity and total phenolic content of Agaricus brasiliensis (Agaricus blazei Murril) in two stages of maturity [J]. Food Chem, 2009, 112: [6] Liang CH, Syu JL, Mau GL. Antioxidant properties of solid-state fermented adlay and rice by Phellinus linteus [J]. Food Chem, 2009, 116: [7] Sarikurkcu C, Tepe B, Yamac M. Evaluation of the antioxidant activity of four edible mushrooms from the Central Anatolia, Eskisehir Turkey: Lactarius deterrimus, Suillus collitinus, Boletus edulis, Xerocomus chrysenteron [J]. Bioresour Technol, 2008, 99: [8]. [D]. :, Shen S. Study on the extraction, separation and purification of the triterpene from the Poria cocos [D]. Wuhan: Central China Agricultural University, [9] Barros L, Ferreira MJ, Queiro s B, et al. Total phenols, ascorbic acid, β-carotene and lycopene in Portuguese wild edible mushrooms and their antioxidant activities [J]. Food Chem, 2003, 103: [10],,,. [J]., 2001, (26)11: Zhang XH, Zhao YF, Chen JM, et al. Content determination of total flavonoids in licorice root [J]. Chin J Chin Mater Med, 2001, (26)11: [11],,,. [J]., 2002, 14(4): 70. Pa LD, Du NS, Cong YY, et al. Determination of triterpene fromteh grape in colorimetric method [J]. Tianjin Pharm, 2002, 14(4): 70. [12],,,. DPPH [J]., 2003, 1: Feng YH, Yu SJ, Li GJ, et al. Study on antioxidant activity of sugarcane extracts by DPPH assay [J]. Sugarcane Canesugar, 2003, 1: [13] Cheung LM, Cheung PCK. Mushroom extracts with antioxidant activity against lipid peroxidantion [J]. Food Chem, 2005, 89: [14] Joao CM, Barreira B, Isabel CFR, et al. Antioxidant activities of the extracts from chestnut flower, leaf, skins and fruit [J]. Food Chem, 2008, 107: [15] Dinis TCP, Madeira VMC, ALmeida LM. Action of phenolic derivates (acetoaminophen, salycilate, and 5-aminosalycilate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers [J]. Arch Biochem Biophys, 1994, 315: [16],,. [J]., 2008, 29(4): Li, HP, Zhang SH, Zhang SH. study on anti-oxidant activity of pholiota nameko polysaccharides [J]. Food Res Dev, 2008, 29(4): 作者简介 ( 责任编辑 : 白洪健 ) 王建明, 副教授, 主要研究方向为大型真菌的研究与开发 wjm1969@163.com 王立安, 教授, 主要研究方向为大型真菌的研究与开发 wlian1965@126.com

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核农学报２０１６３０５０９２００９２５ＪｏｕｒｎａｌｏｆＮｕｃｌｅａｒＡｇｒｉｃｕｌｔｕｒａｌＳｃｉｅｎｃｅｓ９２０文章编号１０００８５５１２０１６０５０９２００６羊栖菜活性组份多糖的体外抗氧化能力比较丁浩淼１２李伟强１汪财生 1 顾筱筱１谢琰１钱国英１王忠华１１浙江万里学院生物与环境学院浙江宁波３１５１００２浙江海洋高效健康养殖协同创新中心