Microsoft Word 最终校稿-1213.doc

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1 4 6 Vol. 4 No Journal of Food Safety and Quality Dec., 2013 涂春蓉 1, 董晓娟 1, 马良 1,2,3*, 张宇昊 (1., ; 2. ( ), ; 3., ) 1,2,3 摘要 :,,, 关键词 : ; ; ; Development of fluorescence enhancement techniques for aflatoxins determination TU Chun-Rong 1, DONG Xiao-Juan 1, MA Liang 1,2,3*, ZHANG Yu-Hao 1,2,3 (1. College of Food Science, Southwest University, Chongqing , China; 2. Laboratory of Quality and Safety Risk Assessment for Agro-products on Storage and Preservation (Chongqing), Ministry of Agriculture, Chongqing , China; 3. Food Engineering and Technology Research Center of Chongqing, Chongqing , China) ABSTRACT: Aflatoxins are the strengthened monitored contaminants of agro-products especially grain and oil products. Trace aflatoxins contamination can cause serious consequences. High sensitivity detection technology is one of effective ways to solve and monitor. Enhancing the natural fluorescence of aflatoxins is one of important ways for improving sensitivity of aflatoxins determination techniques. A variety of traditional fluorescence enhancement technology and the forefront super-molecule fluorescence enhancement techniques were reviewed in this paper. The trend of aflatoxin fluorescence enhancement technology was analyzed. KEY WORDS: aflatoxins; fluorescence enhancement; high sensitivity; super-molecule (aflatoxins, AFs), 1993 I [1] B 1 (aflatoxin B 1, AFB 1 ),, ( AFB 1 ), AFs,, 基金项目 : (2013CB127803) ( ) (XDJK2013B035) ( ) Fund: Supported by National Basic Resaerch Program of China(2013CB127803), the National Natural Science Foundation of China( ), the Fundamental Research Funds for the Central Universities (XDJK2013B035) and National Training Programs of Innovation and Enterpreneurship for Undergraduates( ) * 通讯作者 :,,, zhyhml@163.com *Corresponding author: MA Liang, Associate Professor, Doctor, College of Food Science, Southwest University, No. 2, Tiansheng Road, Beibei District, Chongqing , China. zhyhml@163.com

2 6, : 1791, ( ), , AFB 1 AFs [2] M 1 (aflatoxin M 1, AFM 1 ), 140% [3],, AFs AFs,, ELISA,,, [4-9],,,,, AFs [4-6,10-11] AFB 1 AFG 1 AFs,,,,,,, 1 AFs 的荧光检测 AFs AFs,, AFs, AFB 1 AFB 2 AFG 1 AFG 2 AFM 1 AFM 2 1, AFM 1 AFM 2 AFB 1 AFB 2,, 365 nm, B AFs 425 nm, G AFs 450 nm AFs,, AFs (HPLC) AFs (RP-HPLC),, AFs AFB 1 AFG 1,, AFs, [10-14] Fig. 1 1 AFs Structures of the main aflatoxins

3 AFs 荧光增强技术 AFB 1 AFG 1, AFB 1 AFG 1,, 2.1 传统的化学试剂衍生增强 AFs 荧光 1978 Manabe [14] B 1 B 2, AFB 1 (triflluoro-acetic acid, TFA) (I 2, Br 2 ) [1,5-6,10-15], AFB l AFG l, B 2a G 2a, Takashi [13] TFA, B 1, G 1 B 2a G 2a, 0.02 μg/kg Tarter, C 18, AFB 1, AOAC TFA ; B 2a G 2a,,,, PTFE T, ;, B l G l, 25~50 RP-HPLC [16] AFB 1,,, KOK [17], (Kobra ),,,,, ; Kobra, (per-brominated pyridinium bromine, PBPB) 1993 [18] 2.2 光化学衍生和电化学衍生 AFB 1 G 1 Aura,,, HPLC AFs [19-21] HPLC,,,, AFB 1 AFB 2 AFG 1 AFG 2, µg/kg [22] 2.3 激光诱导荧光 (laser induced fluorescence detector, LIFD),, (laser induced fluorescence, LIF),,, 1992 Cole [23] 1997 Maragos [24-25] 2000 Wei [26] HPCE, LIFD AFs HPCE,,,,,,, AFs, AFB [27], AFs, ; (HPCE-MECC) AFs, AFB 1 LIF-HPCE, RSD<3%, RSD<5%, 84.1%~96.1%, R pg, 0.56 pg, 3, LIFD HPCE,

4 6, : 金属螯合作用增强 AFs 荧光 [1] AFs, AFs, Hg 2+ AFB 1 AFG 1 AFM 1, 1:1, 2 min,, 4, Br 2 2, AFs, AFs Hg 2+, AFs Hg(II) LMCT,,,,,, HPLC [1,15] 2.5 超分子荧光增强环糊精对 AFs 的荧光增强, (cyclodextrins, CDs) AFs, AFs,,,, [28-31] CDs AFB 1 AFG 1 OTA ZEN T-2,, Dall Asta [32] 3 CD 10 CD, AFB 1 AFB 2 AFG 1 AFG 2 AFM 1, AFs CD,,, KI, β-cd, AFs 1:1, AFs CD,, CD, AF CD, AFB 2 AFG 2, AFM 1 CD Dall Asta [32] Amadasi [33] Verrone [34] OTA AFs ZEN,, HPLC Amadasi, AFB 1 AFG 1,,, [33] Franco [35] CDs HPLC AFs Janini [36] Wei [26] Maragos [24-25,37-40] CDs AFs,, β-cd AFB 1, CD AFs, [41-46], CD AFM 1 Chiavaro [40] Franco [35] succinyl-β-cd AFM 1 AFs,,,,,,,,,,,, CD, 三元超分子包合物荧光增强效应 AF [47-50], AFB 1 AFG 1 AFM 1, AFB 1 AFG 1 ( Hg 2+ ),, AFs-Hg 2+ CD,,,,,,, β-cd-hg 2+ AFB 1 15,, AFB 1 [47-49],

5 1794 4, AFs, AFB 1 AFG 1 AFs,,,,,,,,,,, 3 总结与展望 AFs,,,,, β-cd,,,, β-cd ( ) AFs,,, AFs,,, 参考文献 [1]. B 1 [D]., Ma L. Study on Detection Technology For Determination of Aflatoxin B 1 with High Sensitivity [D]. Chinese Academy of Agricultural Sciences, [2]. [EB/OL]. ( )[ ]. tem/2012/ 01/05/ shtml. Wang J. Multiple batches of edible oil was found to exceed the standard aflatoxin [EB/OL]. ( ) [ ]. tem/2012/01/05/ shtml. [3]. M 1 140%[EB/OL]. ( ) [ ]. com.cn/ / html. Zhu Y. A batch of Mengniu pure milk were detected carcinogens aflatoxin M 1 exceed the standard 140%.[EB/OL]. ( ) [ ]. html. [4],. [J]., 2005, 32(5): Liu J, He BY. Research progress on immunochemical analysis methods of aflatoxins [J]. Mod Prev Med, 2005, 32(5): [5],. [J]., 2005, 17(4): Gao XF, Ji R. Analytical methods for aflatoxins in food and feed [J]. Chin J Food Hyg, 2005, 17(4): [6],,,. B 1 [J]., 2005, 27(2): Li PW, Ma L, Yang JE, et al. A review on analytical methods for aflatoxinb 1 in grains and oilseeds products[j]. Chin J Oil Crop Sci, 2005, 27(2): [7] Zhang DH, Li PW, Zhang Q, et al. Development of an ultrasensitive, nanogold probe-based immunochromatographic assay for simultaneous detection of total aflatoxins [J]. Bios Bioe, 2011, (26): [8]. B 1 [D]., Sun XL. Study on Glod Labeled Immunochromatographic Assay for Detection of Aflatoxin B 1 in Foods[D]. Jiangnan University, [9]. [D]., Xie GH. Development of Gold Immunochromatography Assay for Rapid Detection of Aflatoxin B 1 [D]. Jining University, [10] Reiter E, Zentek J, Razzazi E. Review on sample preparation strategies and methods used for the analysis of aflatoxins in food and feed [J]. Mol Nutr Food Res, 2009, 53(4): [11],. [J]., 2005, 15(7): Liu J, He BY. A review of HPLC method for aflatoxins in food products [J]. Chin J Health Lab Technol, 2005, 15(7): [12] Jaimez J, Fente CA, Vazquez BI, et,al. Application of the assay of aflatoxins by liquid chromatography with fluorescence detection in food analysis [J]. J Chromatogr A, 2000, 882: [13] Takahashi DM. Reversed-phase high-performance liquid chromatographic analytical system for aflatoxins in wines with fluo-

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Microsoft Word doc 5 7 Vol. 5 No. 7 2014 7 Journal of Food Safety and Quality Jul., 2014 - AFB 1 周 * 闯, 班晓敏, 刘胜利, 张海彬 (, 210095) 摘要 : 目的 AFB 1 HPLC 方法 AFB 1, HPLC 结果 AFB 1 0.5~80 μg/ml, Y=1095223X+857392, R 2 =0.99884, 0.60