绎 阴 元元 510515 Caco-2 Caco-2 P<0.05 P<0.05 P<0.05 P< 0.05 1 SGLT-1 Caco-2 1 doi: 10.11842/wst.2013.02.013 R285 A Peach resin 3 [1] 1994 2h 3 [2] 20 60 [3] [7~9] [4] 2012-12-24 2013-03-21 元 C1030501 元元 228
2013 绎 Vol.15 No.2 [12] Caco-2 Human colon adenocarcinoma cell lines 1 Sodium glucose cotransporter-1 SGLT-1 Caco-2 SGLT-1 1. DMEM HBSS Gibco [GOPOD ] 2. Millicell-ERS Millipore Transwell 0.4 滋 m Pore size 0.33 cm-2 Corning 3. Caco-2 Caco-2 ATCC 4. [5] 5. [6] 6. Caco-2 2 伊 10 6 cm -2 Transwell 21~23 TEER Caco-2 7. Caco-2 Caco-2 Transwell AP BL HBSS ph=7.4 2 2 37 益 CO 2 20 min HBSS 37 益 0.125~1 滋 g ml -1 HBSS 0.1 ml AP BL 0.7 ml AP AP 1 Caco-2 HBSS 0 10 15 20 25 mmol L -1 1 滋 g ml -1 0 30 60 90 120 min) [GOPOD ] Caco-2 2 1 0.5 0.25 0.125 滋 g ml -1 4 5 20 mmol L -1 HBSS 20 mmol L -1 8. x 依 s SPSS13.0 1. Caco-2 Transwell 229
绎 TEER TEER 21 TEER 600 赘 cm 2 2. 1 滋 g ml -1 10 15 20 25 mmol L -1 P<0.05 1 1 P<0.05 3. 0.5 0.25 0.125 滋 g ml -1 20 mmol L -1 P<0.05 2 2 (P<0.05 [10, 11] SGLT- 1 2 Facilitative glucose 1 x 依 s n=5 mmol L -1 1 0.32 依 0.19 ab 0.92 依 0.51 ab 0.82 依 0.59 ab 1.46 依 0.43 ab 1 1.00 依 0.0.7 2.52 依 0.42 3.42 依 0.17 4.90 依 0.56 2 0.62 依 0.28 ab 1.26 依 0.65 ab 2.42 依 0.67 ab 3.28 依 0.79 ab 2 2.22 依 1.23 5.70 依 1.15 6.80 依 0.99 8.04 依 0.99 3 0.62 依 0.25 ab 3.06 依 0.35 ab 4.58 依 0.31 ab 6.08 依 0.28 ab 3 3.26 依 1.77 5.52 依 2.30 8.22 依 2.58 11.94 依 1.22 4 2.64 依 1.42 ab 4.68 依 1.18 ab 7.64 依 0.81 ab 9.82 依 0.92 ab 4 4.54 依 2.66 7.56 依 1.56 9.94 依 1.29 13.3 依 0.94 1 2 3 4 10 15 20 25 mmol L -1 +1 滋 g ml -1 1 2 3 4 10 15 20 25 mmol L -1 a P<0.05 b P<0.05 10.00 8.00 6.00 4.00 25 mmol/l 20 mmol/l 15 mmol/l 10 mmol/l 12.0 10.0 8.0 6.0 4.0 1 0.5 0.25 0.125 2.00 2.0 0.00 0.0 1 2 230
2013 绎 Vol.15 No.2 2 x 依 s n=5 mmol L -1 1 0.62 依 0.25 b 3.06 依 0.35 b 4.58 依 3.11 b 6.08 依 0.28 b 2 0.72 依 0.13 b 3.18 依 0.36 b 5.30 依 0.16 b 6.66 依 0.17 b 3 0.88 依 0.16 b 3.62 依 0.69 b 5.96 依 0.61 b 7.88 依 0.55 b 4 1.58 依 0.34 b 5.56 依 1.67 b 8.72 依 1.44 b 10.54 依 1.39 b 3.26 依 1.77 5.52 依 2.30 8.22 依 2.58 11.94 依 1.22 1 2 3 4 1 0.5 0.25 0.125 滋 g ml -1 +20 mmol L -1 20 mmol L -1 b P<0.05 transporters 2 GLUT-2 SGLT-1 GLUT-2 Caco-2 SGLT-1 GLUT-2 P<0.05 P<0.05 P<0.05 P<0.05 10 SGLT-1 SGLT-1 1.., 2005 颐 2545. 2.. 1994 颐 165. 3,,,. 2., 2010, 16(5) 颐 8~10. 4,,,.., 2012,18(8) 颐 85~88. 5,,,.., 2008(1) 颐 66~68. 6 SlM JS, Zhao HL, Li DW, et al. Efects of saponins from the root bark of Aralia data on the transport of chondroitin sulfate in Caco-2 cell monolayers and rats. Biol Pharm Bull, 2005, 28(6) 颐 1043~1048. 7,,,..,2009,34(9) 颐 1149~1154. 8 Zhang Z, Zhang WS, Du XF. Hypoglycemic effects of black gluti 原 nous corn polysaccharides on alloxan induced diabetic mice. Euro 原 pean food research and technology, 2010, 230(3) 颐 411. 9,.., 2012, 26(6) 颐 806~809. 10,,. Caco-2 琢 - SGLT-1 GLUT-2 Na + -K + -ATP mrna.,2010, 31(6) 颐 373~376. 11 Gorboulev V, Schurmann A, Vallon V, et al. Na (+)-D-glucose co 原 transporter SGLT1 is pivotal for intestinal glucose absorption and glu 原 cose-dependent incretin secretion. Diabetes, 2012, 61(1) 颐 187~196. 12,,..,2009,32(5) 颐 6~7. 231
绎 Effect of Peach Gum Polysaccharide on Glucose Absorption in Caco-2 Cell Culture Model Xia Yiwei, Wei Liping, Gu Hao, Wang Fei (Traditional Chinese Medicine College of Southern Medical University, Guangzhou 510515, China) Abstract: This study was aimed to investigate the possible mechanism of peach gum polysaccharide affect glucose absorption in Caco-2 cell culture model. Caco-2 cell was used as in vitro drug transport model. Under the influ 鄄 ence of peach gum polysaccharide, the relationship between glucose absorption and time change was observed in order to infer the transepithelial transport of glucose in the peach gum polysaccharide solution. The results showed that under the action of a certain concentration of peach gum polysaccharide, the absorption amount of different concentrations of glucose with the control group showed a significant difference (P < 0.05). It showed that the glucose absorption was inhibited by peach gum polysaccharide. And the lower the glucose concentration is, the more significant the inhibition by peach gum polysaccharide is (P < 0.05). Compared with the control group, dif 鄄 ferent concentrations group of peach gum polysaccharide with the same concentration of glucose showed a signifi 鄄 cant difference (P < 0.05). And the higher the concentration of peach gum polysaccharide is, the more significant the inhibition to the glucose is (P < 0.05). It was concluded that peach gum polysaccharide can inhibit glucose absorption competitively. But whether it can influence or even occupy the glucose absorption sites on the intesti 鄄 nal brush border side of the sodium glucose cotransporter-1 (SGLT-1) in order to reduce the postprandial blood glucose should still be confirmed with more researches. Keywords: Peach gum polysaccharide, diabetes mellitus, glucose, Caco -2 cell culture model, sodium glucose cotransporter-1(sglt-1) 232