绎 330004 3 2010 doi: 10.11842/wst.2014.06.028 R288 A A stragalus mem 原 branaceus Fisch. Bge. var.mongholicus Bge. Hsiao A stragalus membranaceus Fisch. Bge. [1] 22 14 [2] [3] [4] [5] [6] 1 1.1 Ultimate 3000 2013-10-09 2013-10-28 元 201007011 元 元 1364
2014 绎 Vol.16 No.6 Diamonsil -C 18 250 mm 伊 4.6 mm 5 滋 m Waters 2695 2420 Empower Welchrom -C 18 250mm 伊 4.6mm 5 滋 mag245 HH-2 SZ-93 RE-52 KQ3200 1.2 Astragalus membranaceus Fisch. Bge. var.mongholicus Bge. Hsiao 110717-201112 110781-200613 2 2.1 [7] [8] 3 3 [ 约 10 益 D 约 20 益 Y S ] 15 10 g 15 2010 0 3 6 9 12 2.2 2.2.1 Diamonsil -C 18 250 mm 伊 4.6 mm 5 滋 m A 0.2% B 1 1.0 ml min -1 260 nm 30 益 3 000 1 2.2.2 1 ml 50 滋 g 2.2.3 1 g 50 ml 4h 25 ml 5mL 2.2.4 2 4 6 8 10 12 滋 L Y 滋 g X 2 Y = 86.834X-0.232 7 r 2 =0.999 8 n=6 0.1 耀 0.6 滋 g 滋 g 2.2.5 10 滋 L 6 RSD 2.80% RSD 约 3 豫 1 /min A/% B/% 0~20 20 寅 40 80 寅 60 20~30 40 60 1365
绎 min min 1 A B HPLC 2 2.2.6 0 1 2 4 8 12 h 10 滋 L RSD 1.17% RSD 约 3 豫 12 h 2.2.7 6 10 滋 L RSD 1.14% RSD 约 3 豫 2.2.8 6 0.5 g 4mL 10 滋 L 2 99% 95% 耀 105% RSD 2.66% 2.2.9 15 10 滋 L 3 2.3 2.3.1 Welchrom -C 18 250 mm 伊 4.6 mm 5 滋 m 颐 32 颐 68 1.0 ml min -1 60 益 30 psi 30 益 4 000 3 1366
2014 绎 Vol.16 No.6 2 /g /mg /mg /mg /% /% RSD/% 0.500 5 0.190 0.200 0.396 103.0 0.500 1 0.190 0.200 0.385 97.50 0.500 0 0.190 0.200 0.384 97.00 0.500 2 0.190 0.200 0.382 96.00 99.00 2.66 0.500 3 0.190 0.200 0.391 100.5 0.500 4 0.190 0.200 0.390 100.0 3 15 0 3 6 9 12 DZS 0.033 0.029 0 0.018 0 0.010 0 0.009 2 DZL 0.033 0.031 0 0.022 0 0.009 6 0.009 0 YZS 0.033 0.029 0 0.021 0 0.009 0 0.008 8 YZL 0.033 0.029 0 0.023 0 0.012 0 0.009 4 SZS 0.033 0.028 0 0.018 0 0.008 3 0.006 4 SZL 0.033 0.027 0 0.018 0 0.008 9 0.006 6 DFS 0.033 0.030 0 0.017 0 0.009 8 0.010 6 DFL 0.033 0.028 0 0.021 0 0.011 0 0.009 9 DFZ 0.033 0.028 0 0.016 0 0.010 7 0.008 0 YFS 0.033 0.032 0 0.020 0 0.012 0 0.011 1 YFL 0.033 0.030 0 0.023 0 0.014 0 0.010 8 YFZ 0.033 0.029 0 0.020 0 0.010 0 0.008 6 SFS 0.033 0.028 0 0.023 0 0.009 0 0.007 5 SFL 0.033 0.029 0 0.020 0 0.007 7 0.007 5 SFZ 0.033 0.027 0 0.018 0 0.007 7 0.007 5 2.3.2 1mL 0.5 mg 2.3.3 4g 40 ml 4h 10 ml 4 40 ml 2 40 ml 5mL D101 1.5 cm 12 cm 50 ml 40% 30 ml 70% 80 ml 5mL 1367
绎 LS min LS 50.00 40.00 30.00 20.00 10.00 0.00 min 0.00 5.00 10.00 15.00 20.00 25.00 30.00 35.00 40.00 B 3 A B HPLC 2.3.4 4 8 12 16 20 24 滋 L Y 滋 g X Y =1.712 7X+3.748 5 R 2 =1 2 耀 12 滋 g 4 2.3.5 10 滋 L 6 RSD 2.29% RSD<3% 2.3.6 0 2 4 6 8 10 h 20 滋 L 5.5 5.4 5.3 5.2 5.1 5.0 4.9 Y =1.712 7X+3.748 5 R 2 =1 0 0.2 0.4 0.6 0.8 1.0 1.2 4 lg RSD 2.02% RSD<3% 10 h 2.3.7 1368
2014 绎 Vol.16 No.6 6 20 滋 L RSD 2.43% RSD<3 豫 2.3.8 6 2g 0.5 ml 20 滋 L 4 99.06% 95% 耀 105% RSD 1.04% 4 2.3.9 15 20 滋 L 5 3 12 5 6 5 6 15 1/3 耀 2/3 4 /g /mg /mg /mg /% /% RSD/% 2.000 2 0.237 0.250 0.482 98.00 99.06 1.04 2.000 4 0.237 0.250 0.485 99.21 2.000 1 0.237 0.250 0.482 97.82 2.000 5 0.237 0.250 0.488 100.3 2.000 2 0.237 0.250 0.485 99.00 2.000 4 0.237 0.250 0.487 100.0 5 15 % 0 3 6 9 12 DZS 0.063 6 0.057 2 0.043 3 0.033 1 0.024 8 DZL 0.063 6 0.058 1 0.041 7 0.030 8 0.025 2 YZS 0.063 6 0.058 1 0.041 6 0.033 5 0.024 2 YZL 0.063 6 0.052 7 0.040 1 0.028 1 0.017 8 SZS 0.063 6 0.052 0 0.042 7 0.026 9 0.015 9 SZL 0.063 6 0.044 6 0.042 0 0.026 6 0.016 3 DFS 0.063 6 0.057 6 0.045 6 0.032 5 0.026 6 DFL 0.063 6 0.047 3 0.043 4 0.032 8 0.021 2 DFZ 0.063 6 0.044 8 0.042 3 0.032 9 0.020 2 YFS 0.063 6 0.058 3 0.045 3 0.034 6 0.024 9 YFL 0.063 6 0.044 9 0.044 8 0.034 6 0.025 5 YFZ 0.063 6 0.044 6 0.040 0 0.033 5 0.016 1 SFS 0.063 6 0.044 7 0.044 3 0.027 7 0.015 6 SFL 0.063 6 0.044 6 0.041 6 0.027 0 0.016 5 SFZ 0.063 6 0.043 3 0.041 0 0.022 2 0.014 5 1369
绎 5 15 12 2010 0.020% 6 15 12 2010 0.040% 4 25 益 [9] 75% [10] 1370
2014 绎 Vol.16 No.6 1.. : 2010 颐 283~284. 2,,,..,2011,22(2) 颐 144~145,147. 3..,2010,8 (16) 颐 195~196. 4,..,2012,27(11) 颐 2891~2893. 5,,..,2012,28(6) 颐 95~97. 6,,,..,2010,21(11) 颐 2984~2985. 7. 援,2011,4(4B) 颐 75~76 援 8,,..,2013,35(3) 颐 606~609. 9,,,.,2005,28 (6) 颐 464~466. 10..,2007,13(2) 颐 81~84. Effect of Different Package and Storage Conditions on Effective Components of Astragalus Pieces Yu Shuqi, Yi Bingxue, Zhang Jinlian, Zeng Zhaojun, Li Zhiqiang, Gong Qianfeng (Medicine A cademy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China) Abstract: This experimental study was aimed to find the effect of different package and storage conditions on the content of effective components of A stragalus pieces. A stragalus pieces were stored under different storage conditions by using different packaging materials and packaging methods. Every three months, the contents of Calycosin-7-glu 鄄 coside and astragaloside were determined according to the 2010 version of Chinese Pharmacopoeia. With the extend 鄄 edstorage time, the contents of two effective components were significantly decreased. After six -monthstorage, the contents were not consistent with the standard of the pharmacopoeia standards. Room temperature had relatively big influence on the loss of content. The plastic and aluminum paperpackagingwere better than kraft paper packaging. The content ofastragaloside using non vacuum packaging method was relatively higher than the vacuum packaging. Contentunder the conditions of cool storehouse and nonvacuum plastic bags was higher than other packagingmethod. And the changes of both contents were relatively stable. It was concluded that the Astragaluspieces should be packed with non vacuum plastic bags, and stored in a cool and dry place. Keywords: A stragalus, packaging and storagecondition, effectivecomponent, content determination 1371