2 3 4 6 16 24 28 32 HPLC? HPLC 40 (UHPLC) 49 54

80 Paul Upchurch IDEX Health & Science IDEX Health & Science (HPLC) HPLC HPLC HPLC 10 John W. Dolan LC LC/GC 2

UHPLC IVD UHPLC John W. Batts, iv IDEX Health & Science

HPLC LC-MS UHPLC 4

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? Paul Upchurch HPLC HPLC HPLC HPLC 6

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1/4-28 1 1/4 28 24 8

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M6 1 M6 6 1 1 10-32 1/4-28 5/16-24 1/4 6.35 5/16 24 1/4 6 24 / 1/4 6

2 www.idex-hs.com 11

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4 22 www.idex-hs.com 13

PEEK TM ETFE PCTFE PEEK ETFE FEP PFA PEEK : 41 14

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16

5 1. 2. 5 www.idex-hs.com 17

3. 6 6 18

HPLC 4,000psi 276bar UHPLC 15,000psi 1,034bar HPLC UHPLC 13 3/4 www.idex-hs.com 19

1/4 1/4 : HPLC UHPLC 7 X X X X 7 51 53 X = 0.080" (2.0mm) X = 0.090" (2.3mm) X = 0.130" (3.3mm) X = 0.170" (4.3mm) 0.080" (2.0mm) 0.170"(4.3mm) 20

HPLC : www.idex-hs.com 21

41 8 22

17 5 9 www.idex-hs.com 23

1/4-28 1/4 1/4 28 10a 1/4 24

1/4 10a 0 1/4 10a 1/4 7 4 10b 1/4 0 1/8 1/4 10B 0 1/4 1/2 3/4 1 www.idex-hs.com 25

20 1/2 (12.7 ) 24 5/16 (7.9 ) 28 1/4 (6.4 ) 32 40 3/16 (4.8 ) 10 9/64 (3.6 ) 6 9/64 (3.6 ) 6 M6 M6 1 6 M6 1 1 10 7 10 M4 0.7 26

6-40 6-32 10-32 1/4-28 5/16-24 1/2-20 M4 x 0.7 M6 x 1 www.idex-hs.com 27

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Delrin TFA LC-MS PEEK PEEK 61 www.idex-hs.com 29

? 1? 30

1,000 psi (69bar) 10,000psi 689bar 6,000psi(414bar) UHPLC 15,000 psi 1,034bar www.idex-hs.com 31

HPLC? HPLC? HPLC HPLC High Performance Liquid Chromatography P HPLC P HPLC 1960 HPLC HPLC 32

HPLC HPLC HPLC : 11 1 6 2 ( ) : : 11 6 5 5 4 1 4 3 3 2 ( ) www.idex-hs.com 33

HPLC? HPLC 20 40 HPLC HPLC C18 C18 MS NMR HPLC 34

12 0 10 20 ( - : HPLC www.idex-hs.com 35

HPLC? 13 HPLC HPLC 36

HPLC HPLC : 54 (BPR) ( BPR 57 www.idex-hs.com 37

HPLC? 1,000psi 69bar 6,000psi 400bar! HPLC 1/4-28 1/8 3.2 HPLC 10-32 1/16 1.6 38

1/4-28 1/16 OEM 1/16 10-32 HPLC 14 HPLC www.idex-hs.com 39

HPLC UHPLC HPLC 1/16 1.6mm 1mL/min 20µl 360µm 44 41 40

1/16 1/16 HPLC 1/32 360 1/16 1/16 www.idex-hs.com 41

15 1/16 1/16 15 16 42

/ 17 1/16 X X www.idex-hs.com 43

Upchaurch Scientific's NanoPorts 44

www.idex-hs.com 45 0.002 19

UHPLC 2004 UHPLC UHPLC 15,000psi 1,034bar UHPLC UHPLC UHPLC 46

2 UHPLC 3) UHPLC UHPLC www.idex-hs.com 47

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/ / 18 vs. 18 www.idex-hs.com 49

1/16 1.6 PEEK 1/8 3.2 FEP PFA PEEK 1/4-28 M6 5/16-24 1/16 1.6 1/8 3.2 1/4-28 1/16 1/8 50

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BPR) HPLC ( ) Mobile Phase Degassing Why, When, and How John W. Dolan, LC-GC Volume 17 Number 10,1999 10. 54

HPLC 37 18% 30% www.idex-hs.com 55

56

BPR BPR BPr? HPLC BPR BPR BPR BPR BPR BPR BPR HPLC BPR HPLC www.idex-hs.com 57

( psi ) P = ( 9.86 x 10-8 )( F L V d 4 ) P = psi V = cp F = / d = L = x 25.4 / x 2.54 / x 25.4 / x l000 / (( /2) x 2.54 / )2 x ( / ) x 2.54 / x 1000 / 3 (( /2) x 2.54 / )2 x ( / ) x 1 / x 1000 / 3 ( x 9 / 5) + 32 ( - 32) x 5 / 9 psi bar psi x 0.06894757 psi psi x 0.06804596 psi mpa psi x 0.00689476 psi torr psi x 51.7150733 58

www.idex-hs.com/materials Delrin (acetal ) Delrin ph C 60 FeP fluorinated ethylene-propylene ) PFA(perfluoroalkoxy alkane ) PTFE HPLC PFA FEP FEP PFA C FEP PFA 80 FEP 50 PFA 80 Halar ectfe ethylene-chlorotrifluoroethylene - ) Halar Halar PTFE C 50 PCTFe polychloro-trifluoroethylene PCTFE THF C 80 PeeK polyetheretherketone PEEK PEEK PEEK THF DMSO C 125 100 Polypropylene ( ) IVD C 40 40 www.idex-hs.com 59

PPs polyphenylene sulfidepps PPS ph C 50 radel polyphenylsulphoneradel Radel Radel C 100 Ultem PEI (polyetherimide Ultem Ultem ISO10992 FDA USP VI C 125 Vespel polyimide Vespel ph Vespel Vespel C 200 etfe ethylene-tetrafluoroethylene - ETFE ETFE ETFE C 80 80 uhmwpe ultra-high molecular weight polyethyleneuhmpe itunes C 50 60

Delrin Halar PCTFE PEEK Perfluoroelastomer Polypropylene PPS 1 Radel R FEP / PFA Tefzel 2 UHMWPE Ultem R R 1 R R R NR R M R R NR R M R M M M NR M M R R M M R R 1 R R R M R M R R M M R R 1 R R R R R M R R R M R M M M R NR R M R R M M M M R R R R R M R M M N/A R R R R R M R R R R M M NR R R M R M R R R R M M NR R R M R M M M R M M M NR R R R R R R R R R R M R R R M R M R M R R M M * n/a n/a n/a n/a C FEP PFA 60 N/A 80 125** 200*** 40 50 N/A N/A 80 80 50 N/A N/A 50 N/A 100** N/A N/A 100*** 50 80 80 N/A 125 1 2 FEP PFA R M Upchurch Scientific NR N/A * ** PEEK IDEX Health & Science *** Radel100 C *** PEEK www.idex-hs.com 61

1. 2. 3. IDEX Health & Science 4. 5. 6. IDEX Health & Science www.idex-hs.com, 1. 2. 3. 4. 62

BPr 1/16 LC-MS x Upchurch Scientific HPLC 1/4-28 M6 www.idex-hs.com 63

HPLC High Performance Liquid Chromatography oem Original Equipment Manufacturer (. T uhplc Ultra High Performance Liquid Chromatography 15,000psi 1,034 bar ( 316 HPLC 64