7 5 Vol. 7 No. 5 2016 5 Journal of Food Safety and Quality May, 2016 张宏康 1, 王中瑗 2, 许佳璇 1, 林小可 1, 李蔼琪 (1., 510225; 2., 510300) 1 摘要 :,,,,,,, : X --,,,,, 关键词 : ; ; Research progress on analysis technologies of heavy metals in foods ZHANG Hong-Kang 1, WANG Zhong-Yuan 2*, XU Jia-Xuan 1, LIN Xiao-Ke 1, LI Ai-Qi 1 (1. College of Light Industry and Food, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China; 2. South China Sea Environment Monitoring Center, State Oceanic Administration, Guangzhou 510300, China) ABSTRACT: Cd, Cr, Pb, As, Hg and other heavy metal elements do great harm to human health. They can lead to variety of diseases, even cancer. The content of heavy metal in food often exceeds standards, and it is a threat to human health. Therefore, the detection of heavy metal content in food is necessary. This article provided an overview of commonly used detection method for heavy metal content in food, such as atomic fluorescence spectrometry, X-ray fluorescence spectrometer, inductive coupling plasma-mass spectrometry, inductive coupling plasma-atomic emission spectrometry and atomic fluorescence spectrometry, and their characteristics were introduced, outlining their strengths and weaknesses. Application examples about detection of heavy metals in food by these methods were introduced, so as to provide a reference for the detection of heavy metals in food. The development of detection methods for heavy metals was also prospected. KEY WORDS: food; heavy metals; detection methods 基金项目 : (41406093)(2013513)(1328) (2013KJCX0103)(2013B090600033)(2016A020210138) Fund: Supported by the National Natural Science Foundation of China (41406093), Youth Fund of State Oceanic Administration(2013513), Director Fund of NCSB(1328), Educational Commission of Guangdong Province Technology Project (2013KJCX0103), Guangdong Province Science and Technology Plan Project (2013B090600033) and Guangdong Province Science and Technology Plan Project (2016A020210138) * 通讯作者 :,,, E-mail: zhongyuan764@126.com *Corresponding author: WANG Zhong-Yuan, Ph.D., Engineer, South China Sea Environment Monitoring Center, State Oceanic Administration, Guangzhou 510300, China. E-mail: zhongyuan764@126.com
5, : 1845 1 引言, 81,,,,,,,,,,,,,,,, [1], 4 5, 45, [2],,,, : (Hg) (Cd) (Cr) (Pb)(As)(Zn)(Sn) [3],,, (atomic absorption spectrometry, AAS) (atomic fluorescence spectrometry, AFS) -(inductive coupling plasma-mass spectrometry, ICP-MS) X (X-ray fluorescence spectrometer, XRF)- (inductive coupling plasma-atomic emission spectrometry, ICP-AES) [4-14],,,, 2 检测食品中重金属常用方法研究进展 2.1 原子荧光光谱法 (AFS)(AES) (AAS), (),, 20,,,, [15,16] [17],,,,, [18],, [19] -- 3 AsHg Pb,,,,,,,,,,, 2.2 X 射线荧光光谱法 X, [20] X, [20], X X, X, X [21] X, X,,, X, X,,, 2.3 电感耦合等离子体 - 质谱 (ICP-MS) 法 -,,,,
1846 7, [22] [23], -,,, [24] -, [25] - 18,,, -,,,,,,,, 2.4 电感耦合等离子体 - 原子发射光谱 (ICP-AES) 法 -, [30],,, (),,,,, [26-29] [31] -- 8 [32] --, [33], - 5 -, -,,,, -,, 2.5 原子吸收光谱法 (atomic absorption spectroscopy, AAS),, 珺 [34] (Pb)(Cd)(Cr)(Hg)(As), (FAAS)(GFAAS) (HGAAS),, [35] 9, [36] -, [37] -, FeCuCaMnZn5 PbCd,,,,,,,,, 2.6 各类方法的适用场所 特点以及局限性小结 1
5, : 1847 Table 1 [38-41] 表 1 各类检测方法的适用场合以及优缺点 The applicable occasions, advantages and disadvantages of each kind of detection methods,,,,,,,,,,,,, X,,,,, X,,,, ppm,,,,,,,,,,,,,,,,, ppt,,,,,,, ICP-MS,, 3 展望,,,,, [42-48],,,,,,, [49],,,,,,,,,,, [50],,,
1848 7 参考文献 [1],,. [J]., 2014, 4: 325329. Rong LP, Xu YY, Jiang XY. Heavy metal poisoning and renal injury in children [J]. Chin J Contemp Pediatr, 2014, 4: 325329. [2],,,. [J]., 2013, 42(2): 355359. Su SP, Xu F, Cao H, et al. Perspective in the rapid methods for the detection of heavy metals [J]. Appl Chem Ind, 2013, 42(2): 355359. [3]. [J]., 2010, 4:8586. Han Y. Pollution harm of heavy metal for foods and suggestion of pollution control [J]. Jilin Veget, 2010, 4: 8586. [4],,. [J]. (), 2003, 7:430433. Zhuang HR, Liu CZ, Chen JC, et al. Recent advances of AAS determination of lead in China [J]. PTCA, 2003, 7: 430433. [5]. [J]., 2007, 6: 698702. Huang JH. Evolution of Cd determination by atomic absorption spectrometry [J]. Miner Resour Geol, 2007, 6: 698702. [6],. [J]., 2003, 1: 94100. Li B, Yang HX. Recent advances in inductively coupled plasma mass spectrometry [J]. Chin J Anal Lab, 2003, 1: 94100. [7]. / [D]. :, 2013. Nie XD. Research on collision /reaction cell inductively coupled plasma mass spectrometry for analysis of food samples [D]. Changsha: Central South University, 2013. [8],,,. - [J]., 2013, 30(4): 18531855. Yang RC, Zhang W, Yuan P, et al. Determination of total chromium in foods by ICP-MS [J]. Chin J Spec Lab, 2013, 30(4): 18531855. [9],,,. X [J]., 2013, 34(24): 254257. Wang DX, Jiang ZM, Tian R, et al. Determination of calcium in milk powder by energy dispersive X-ray fluorescence spectrometry [J]. Food Sci, 2013, 34(24): 254257. [10],,,. 9 [J]., 2006, 35(2): 225227. Li G, Li CY, Song LJ, et al. Analysis of nine kinds of elements contents in the milk powder by inductively coupled plasma-atomic emission spectrometry [J]. J Hyg Res, 2006, 35(2): 225227. [11]. [D]. :, 2013. Tan FW. Determination of iodine, chromium in foods and mrcury in antimony ingot by atomic fluorescence spectrometry [D]. Nanning: Guangxi University, 2013. [12]. [D]. :, 2012. Wang YL. Study of speciation of arsenic and mercury in soil sample by atomic fluorescence spectroscopy [D]. Changchun: Jilin University, 2012. [13],,. [J]., 2011, 5: 576579. Wang XQ, Hou XL, Yang HL. Simultaneous quantification of silver, copper, lead and zinc in lead-zinc ores by inductively coupled plasma-atomic emission spectrometry [J]. Rock Min Anal, 2011, 5: 576579. [14]. [J]., 2014, 11: 110. Zheng GJ. New advances in inductively coupled plasma atomic emission spectrometric instruments and methods [J]. Metal Anal, 2014, 11: 110. [15]. [D]. :, 2012. Li RS. Development of atomic fluorescence mercury analyzer and methods review of atomic fluorescence spectrometry simultaneously measured the multiple elements [D]. Xi an: Northwest University, 2012. [16],,. [J]., 2013, 3: 358376. Li G, Hu SX, Chen LL. Innovation and development for atomic fluorescence spectrometry analysis [J]. Rock Miner Anal, 2013, 3: 358376. [17],,. - [J]., 2008, 25(5): 388392. Wang ZJ, You HD, Wu ZG. Determination of heavy metals in the traditional Chinese medicines by microwave digestion-atomic fluorescence spectrometry [J]. J Shenyang Pharm Univ, 2008, 25(5): 388392. [18],,. - [J]., 2011, 17(24): 3233. Xing JB, Cao H, Wang GJ. Determination arsenic and mercury in eggs and egg product by wet digestion -atomic fluorescence spectrometry [J]. Coutemp Med, 2011, 17(24): 3233. [19],,,. - [J]., 2014, 4: 151154. Ye HX, Tan Z, Liu XQ, et al. Determination of arsenic, mercury and lead in radix puerariae by atomic florescence spectrophotometry with wet digestion [J]. Food Sci, 2014, 4: 151154. [20],,,. X [J]., 2011, 41(6): 605611. Liu YD, Wan CL, Sun XD, et al. Application of X-ray fluorescence spectrometer technique in detection of heavy metal [J]. Laser Infrared, 2011, 41(6): 605611. [21],,. X- [J]., 2014, 26(6): 551554. Peng XK, Wang H, Yuan LJ. Rapid screening of cadmium in rice by X-ray fluorescence spectrometer [J]. Chin J Food Hyg, 2014, 26(6): 551554. [22],,,. (ICP-MS) [J]., 2012, 2: 15. Li JY, Shi L, Lu SH, et al. Progress of inductively coupled plasma mass spectrometry and its hyphenated techniques [J]. Chin J Inorg Anal Chem, 2012, 2: 15. [23],,,. [J]., 2014, 42(18): 5950, 5973. Liu WM, Liu YZ, Wang XF, et al. Determination of heavy metals in sugar
5, : 1849 cane juice by inductively coupled plasma mass spectrometry [J]. J Anhui Agric Sci, 2014, 42(18): 5950, 5973. [24],,,. [J]., 2011, 30(8): 8891. Li X, Yu JJ, Li B, et al. Determination of Pb element in blood and urine of lead-exposed pigs by inductively coupled plasma-mass spectrometry [J]. Chin J Anal Lab, 2011, 30(8): 8891. [25],,,. - 18 [J]., 2015, 51(3): 8790. Xia YJ, Zhang H, Shu YL, et al. Determination Method of 18 Metals in Wine by ICP-MS [J]. Food Ferment Technol, 2015, 51(3): 8790. [26],,. 5 [J]., 2009, 6: 226228. Huon JL, Liu MG, Lou GZ. Determination of five rare earth elements in tea by inductive coupling plasma-optical emission spectrometry [J]. Food Sci, 2009, 6: 226228. [27],,,. (ICP-AES) [J]., 2009, 2: 6164. Qi QG, Zhao Y, Gao W, et al. Detection of trace elements in the milk and milk products by ICP-AES [J]. Dairy Ind, 2009, 2: 6164. [28],. [J]., 2008, 9: 248250. Deng ZY, Li JJ. Determination of aluminum in food by inductively couple plasma atomic emission spectrometry [J]. Food Sci Technol, 2008, 9: 248250. [29],,,. 9 [J]., 2006, 2: 225227. Li G, Li CY, Song LJ, et al. Analysis of nine kinds of elements contents in the milk powder by inductively coupled plasma-atomic emission spectrometry [J]. J Hyg Res, 2006, 2: 225227. [30],,,. [J]., 2015, 26(3): 5658. Cheng XH, Yang QH, Chen F, et al. The research progress of heavy metals in food safety detection method [J]. Jiangsu J Prev Med, 2015, 26(3): 5658. [31],,,. - 8 [J]., 2012, 35(6): 117120. Ye R, Liu FZ, Liu J, et al. Determination of contents of Cu, Mn, Fe, Zn, Ca, Mg, K and Na in rice using microwave digestion and inductively coupled plasma-optical emission spectrometry [J]. Food Sci, 2012, 35(6): 117120. [32],,,. - [J]., 2008, 1: 2124. Lin L, Yang YL, Zhou AF, et al. Determination of total boron in foods by inductively coupled plasma-optical emission spectrometry with microwave digestion sample preparation [J]. Rock Miner Anal, 2008, 1: 2124. [33],. - [J]., 2013, 5: 303307. Guo LP, Tang J. Determination of trace elements in tea by microwave digestion and inductively coupled plasma atomic emission spectroscopy [J]. Food Sci Technol, 2013, 5: 303307. [34] 珺,,. [J]., 2012, 7: 304309. Cao J, Zhao LJ, Zhong RG. Progress in determination of heavy metals in foods by atomic absorption spectrometry [J]. Food Sci, 2012, 7: 304309. [35],,,. - 9 [J]., 2010, 9: 17071712. He PW, Du G, Zhao HY, et al. Determination of five heavy metals in nine Chinese medicines by atomic absorption spectrometry coupled with microwave digestion [J]. Chin J Pharm Anal, 2010, 9: 17071712. [36],. [J]., 2011, 1: 7274. Zhang H, Tang J. Determination of Fe, Mn, Cu, Pb and Cd in vegetables by atomic absorption spectrometry [J]. Chin J Spect Lab, 2011, 1: 7274. [37],,,. 7 [J]., 2006, 5: 962965. Yu L, Peng XJ, Li YB, et al. Determination of seven trace elements in tea samples by atomic absorption spectrometry [J]. Chin J Spect Lab, 2006, 5: 962965. [38]. [J]., 2010, 6: 3943, 49. Luo XZ. Application of PSO and time-based sequence analysis in intrusion detection [J]. Qual Technol Sup Res, 2010, 6: 3943, 49. [39],,,. [J]., 2014, S1: 237241, 362. Wang YH, Wang YF, Chen H, et al. Detect methods of heavy metals in seawater and treatment technology exploration [J]. Environ Sci Technol, 2014, S1:237241, 362. [40],,. [J]., 2015, 23: 5053. Leng JS, Gao XM, Wang LX. Hazards of heavy metal pollution in food and progress in study on analysis technologies [J]. Farm Prod Proc, 2015, 23: 5053. [41],,,. [J]., 2015, 4: 724728. Yuan M, Wu JC, Yu JS, et al. Progress on detection methods of heavy metals in water [J]. Appl Chem Ind, 2015, 4: 724728. [42]. [D]. :, 2011. Qi SM. Construction of the system of quality safety management of agricultural products [D]. Yangzhou: Yangzhou University, 2011. [43] [43] Suturović Z, Kravić S, Milanović S, et al. Determination of heavy metals in milk and fermented milk products by potentiometric stripping analysis with constant inverse current in the analytical step [J]. Food Chem, 2014, 155: 120125. [44] Behbahani M, Hassanlou PG, Amini MM, et al. Application of solvent-assisted dispersive solid phase extraction as a new, fast, simple and reliable preconcentration and trace detection of lead and cadmium ions in fruit and water samples [J]. Food Chem, 2015, 187: 8288. [45] Mohammad HM, Mitra AD, Mahmoud RS, et al. Solid phase extraction of trace amounts of silver, cadmium, copper, mercury, and lead in various food samples based on ethylene gl;ycol bis-mercaptoacetate modified 3-(trimethoxysilyl)-1-propanethiol coated Fe3O4 nanoparticles [J]. Food Chem, 2014, 151: 300305. [46] Akinyele IO, Shokunbi OS. Comparative analysis of dry ashing and wet
1850 7 digestion methods for the determination of trace and heavy metals in food sample [J]. Food Chem, 2015, 173: 682684. [47] Liu B, Huang Q, Cai HJ, et al. Study of heavy metal concentrations in wild edible mushrooms in Yunnan province, China [J]. Food Chem, 2015, 188: 294300. [48] Tarighat MA. Orthogonal projection approach and continuous wavelet transform-feed forward neural networks for simultaneous spectrophotometric determination of some heavy metals in diet samples [J]. Food Chem, 2016, 192: 548556. [49]. [D]. :, 2008. Xiao Y. Study on the coupled technique of atomic absorption spectrometry and its application[d]. Guilin: Guangxi Normal University., 2008. [50],,. [J]., 2010, 3: 953957. Zhang L, Wang XY, Li B. Application of microwave digestion technology in metal analysis [J]. Chin J Spect Lab, 2010, 3: 953957. 作者简介 ( 责任编辑 : 金延秋 ) 张宏康, 副教授, 主要研究方向为食品质量与安全 E-mail: zhkuzhk@163.com 王中瑗, 工程师, 主要研究方向为流动注射与高灵敏度检测器联用的研究 E-mail: zhongyuan764@126.com 功能性食品研究 专题征稿函 投稿方式 : 食品安全质量检测学报 编辑部