8 1 Vol. 8 No. 1 2017 1 Journal of Food Safety and Quality Jan., 2017 2011~2015 杨修军, 赵薇 *, 刘桂华, 黄鑫, 王慧, 张炜煜, 石奔 (, 130062) 摘要 : 目的, 方法 GB 4789.30-2010 2013 结果 2011~2015 5093 11 294, 5.77% 9,, 12.43% 5.88% 5.43%, 结论, 关键词 : ; ; Analysis on the monitoring data of Listeria monocytogenes in food of Jilin Province in 2011~2015 YANG Xiu-Jun, ZHAO Wei *, LIU Gui-Hua, HUANG Xin, WANG Hui, ZHANG Wei-Yu, SHI Ben (Jilin Provincial Center for Disease Control and Prevention, Changchun 130062, China) ABSTRACT: Objective To investigate the status of market food contaminated with Listeria monocytogenes (L.M.) in Jilin province, in order to provide scientific data for the prevention of foodborne disease and food safe. Methods According to GB 4789.30-2010 National food safety standard Food microbiological examination: Listeria monocytogenes and Manual for China national food contamination and harmful factors risk monitoring in 2013, the risks of L.M. from foods between different areas, different kinds and different categories and sampling locations were monitored. Results A total of 5093 samples of 11 kinds of food were detected and analyzed. Among those, total 294 strains of L.M were isolated, and the total positive rate was 5.77%. The positive rates of Baishan, Changchun and Siping cities were higher than other regions. The positive rates of meat and meat products, quick-frozen rice flour products and animal aquatic products were much higher than others, which were 12.43%, 5.88% and 5.42%, respectively. Compared with catering and service industry, the pollution rate of circulation was much higher. The positive rate of bulk and pre-packaged food had no significant difference. Conclusion The market foods 基金项目 : (2014Q012) Fund: Supported by the Youth Scientific Research Program of Jilin Provincial Health and Family Planning Commission Project (2014Q012) * 通讯作者 :,, E-mail: weizhao81226@126.com *Corresponding author: ZHAO Wei, Associate Chief Technician, Jilin Provincial Center for Disease Control and Prevention, Changchun 130062, China. E-mail: weizhao81226@126.com
106 8 contaminated with Listeria monocytogenes show varying degrees in all regions of Jilin province. The supervision and regulation of the production, processing, transportation, sales and catering services with high risk of contamination should be strengthen. KEY WORDS: food; Listeria monocytogenes; food safety 1 引言 (Listeria monocytogenes, L.M., ),, [1],, [2],, 20%~30% [3-5], 2 材料与方法 2.1 样品来源 9 ( ) 2011~2015,, 5093 2.2 培养基及试剂 ( ); API ( ) ( ); PALCAM ( OXIOID ) 2.3 检测指标及方法 GB 4789.30-2010 [6], 2013 [7] 2.4 菌株的初检与复核 PALCAM API, 2.5 数据统计分析 Excel 2007, SPSS17.0 X, P<0.05 3 结果与分析 3.1 2011~2015 年间吉林省食品监测中单增李斯菌污染检测结果 2011-2015 5093, 294, 5.77%, 2014 ; 2015 2012 2011 6.00%(75/1251), 2013 5 (X 2 =42.2662, P<0.05), 2013 1 3.2 不同采样地区的食品中单增李斯特菌监测结果 5093 9 ( ),,, 9,, X 2 =55.4546, P<0.05 3.3 不同类别食品单增李斯特菌监测结果 11, :, 100,,, ( X 2 =5.6738 17.7414, P 0.05), ( X 2 =0.0463, P>0.05), 3.82%, 2.59%,, 1.48%, 3 ( X 2 =5.4439, P>0.05) 2 3.4 不同采样地点类型食品中单增李斯特菌监测结果 5093,, 4.42% 6.37%, ( X 2 =7.5298, P<0.05), /,, / 5
1, : 2011~2015 107 Table 1 表 1 不同年份及采样地区的食品中单增李斯特菌监测结果 Monitoring results of L.M. from foods in different years and regions 2011 2012 2013 2014 2015 8.92%(42/471) 8.63%(12/139) 12.77%(12/94) 2.67%(2/75) 15.91%(14/88) 2.67%(2/75) 8.82%(59/669) 10.07%(14/139) 4.00%(3/75) 2.56%(5/195) 19.10%(17/89) 11.70%(20/171) 8.77%(54/616) 7.19%(10/139) 18.09%(17/94) 0.00%(0/75) 15.38%(22/143) 3.03%(5/165) 6.44%(31/481) 10.79%(15/139) 9.57%(9/94) 0.00%(0/75) 6.19%(7/113) 0.00%(0/60) 5.34%(26/487) 4.32%(6/139) 0.00%(0/94) 10.67%(8/75) 3.23%(3/93) 10.47%(9/86) 4.12%(21/510) 2.88%(4/139) 2.13%(2/94) 2.67%(2/75) 2.61%(3/115) 11.49%(10/87) 3.76%(20/532) 3.60%(5/139) 1.06%(1/94) 1.48%(2/135) 5.49%(5/91) 9.59%(7/73) 3.59%(25/697) 4.32%(6/139) 0.00%(0/75) 0.51%(1/195) 3.57%(5/140) 8.78%(13/148) 2.54%(16/630) 2.16%(3/139) 5.32%(5/94) 1.03%(2/195) 3.33%(3/90) 2.68%(3/112) 5.77%(294/5093) 6.00%(75/1251) 6.06%(49/808) 2.01%(22/1095) a 8.21%(79/962) 7.06%(69/7.06) : a: 2013 2011 ( X 2 =23.4061, P<0.05), 2012 ( X 2 =21.2868, P<0.05), 2014 ( X 2 =42.2014, P<0.05), 2015 ( X 2 =31.3995, P<0.05) Table 2 表 2 不同种类食品中单增李斯特菌监测结果 Monitoring results of L.M. from different kinds of foods % 1360 169 12.43% ( ) b 155 32 20.65% c 337 62 18.40% 118 11 9.32% 750 64 8.53% 153 9 5.88% 461 25 5.42% 112 4 3.57% 349 21 6.02% 1674 64 3.82% d 607 47 7.74% 158 4 2.53% 605 12 1.98% 173 1 0.58% 131 0 0.00% 15 1 6.67% 50 3 6.00% 733 19 2.59% 270 4 1.48% 360 0 0.00% 15 0 0.00% 2 0 0.00% 5093 294 5.77% : b: ( ) ( X 2 =6.4731 19.8720, P 0.05) c: ( X 2 =5.3443 22.077, P 0.05) d: ( X 2 =21.7040, P<0.05)
108 8 Table 3 表 3 不同采样地点类型食品中单增李斯特菌监测结果 Monitoring results of L.M. from foods in different kinds of collecting locations % 1560 69 4.42 _ / 651 40 6.14 _ / _ 55 3 5.45 _ / _ 4 0 0.00 _ / _ 439 27 6.15 _ / _ 153 10 6.54 _ 207 6 2.90 _ _ 2 0 0.00 _ _ _ 27 1 3.70 _ _ _ 5 0 0.00 _ _ _ 22 0 0.00 _ _ _ 30 1 3.33 _ _ _ 121 4 3.31 _ 303 8 2.64 _ ( ) 120 6 5.00 _ 167 8 4.79 _ 112 1 0.89 3533 225 6.37 _ _ 91 5 5.49 _ _ / 465 5 1.08 _ _ / 672 34 5.06 _ _ e 326 62 19.02 _ _ ( ) 381 15 3.94 _ _ f 1406 97 6.90 _ _ 102 6 5.88 _ _ 76 0 0.00 _ _ 14 1 7.14 5093 294 5.77 : e: /, ( X 2 =46.6216 10.0318 49.1973 41.1731, P 0.05); f: ( X 2 =4.4768, P<0.05), / ( X 2 =5.3133, P<0.05),,,,, / 3 3.5 不同包装类型食品中单增李斯特菌监测结果 5093 : ( ), 6.07% (240/3959),, 4.76% (54/1134),,
1, : 2011~2015 109 ( X 2 =2.7398, P<0.05) 4 讨论, 2011~2015, 2014 2015 2011 2012 ( 2013 2.01%), 2013, 5 2010~2011 1.11% [8], 2013, ( X 2 =4.0032, P<0.05) 2014 4.82%, ( X 2 =9.9826, P<0.05) [9] 2011 1441, 2.67% [10] 2011 6.00%, X 2 =27.3590, P<0.05 2010 5.91% [11], 2011,,,,,,,,, ( ) 20.65% 18.40% 9.32% [9], 2010~2014,, 8.88% 2011, 7.23%(12/166) [10],, ( ),,,,, ( ) ( ) ( ) [12],,,, [13],, [14],, (centers for disease control and prevention, CDC) :,,, ( ) [15],,,,, /,,, 19.02%,, /,,, 5 结论,,,, 参考文献 [1] Ryser ET, Marthe H. Listeria, listeriosis and food safety [M]. Florida: CRC Press, 2007. [2] World Health Organization. Foodborne listeriosis [R]. Geneva: WHO, 1988: 421 428. [3] Food and Agriculture Organization of the United Nations and World Health Organization. Risk assessment of Listeria monocytogenes in ready to eat foods-technical report, in microbiological risk assessment series, No 52004 [R]. Rome: FAO/WHO, 2004: 1 60. [4] Centers for Disease Control and Prevention (CDC). Outbreak of Listeria monocytogenes infections associated with pasteurized milk from a local dairy-massachusetts [J]. Morbid Mortality Weekly Report, 2008, 57(40): 1097 1100.
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