Microsoft Word 赵丽元-二校

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1 6 4 Vol. 6 No Journal of Food Safety and Quality Apr., 2015 赵丽元 *, 秦峥, 吴世杰, 李寅生, 丁红雨 (, ) 摘要 : 目的,, 方法 ~2014 1, 150,, 结果 150 9, 1, ( )10, ()1, 93.3%, 结论, 关键词 : ; ; ; Evaluation of contamination status and safety of pathogenic organisms in edible raw aquatic products in Liaoning province ZHAO Li-Yuan *, QIN Zheng, WU Shi-Jie, LI Yin-Sheng, DING Hong-Yu (Dalian Institute for Drug Control, Dalian , China) ABSTRACT: Objective To investigate the contamination of pathogens of edible raw aquatic in Liaoning province, and provide accurate information for evaluation, analysis and management of the present food safety situation in Liaoning. Methods Totally 150 samples of edible raw aquatics were collected from all sizes of restaurants in Dalian, Panjin and Yingko of Liaoning province from December 2013 to January Salmonella, Shigella, Staphylococcus aureus, Listeria monocytogenes, Aerobic plate count, Coliform bacteria, Vibrio parahaemolyticus and parasite metacercaria were detected by the national food safety standards. Results Pathogenic microorganisms were detected, including 9 strains of Vibrio parahaemolyticus, 1 strain of Listeria monocytogenes, 10 strains of Staphylococcus aureus and 1 strain of Aerobic plate count. The qualified rate was 93.3%. Conclusion Edible raw aquatics were contaminated by prevalent pathogenic organisms in Liaoning province. It is necessary to strengthen the management of food hygienic and promulgate safe practices for edible raw aquatics. KEY WORDS: aquatic product; risk monitoring; pathogen; food safety 1 引言,,,, 基金项目 : ((2013)239) Fund: Supported by the Risk Monitoring of Edible Raw Aquatic Products in Liaoning Province ((2013)239) * 通讯作者 :,,, [email protected] *Corresponding author: ZHAO Li-Yuan, Pharmacist, Dalian Institute for Drug Control, Dalian , China. [email protected]

2 4, : 1435 [1], [2] [3], 24.3% 13.1%,,,, 150,, ( ) () (), 2 材料与方法 2.1 材料样品收集 ( ) , 33%; 63, 42%; 37, 25% 2 ( ), , 4 10,, 3 20 ( 1) 培养基和试剂 BPW; TTB; SC; BS; XLD; TSI; ; ; ; ; 3% ; ; 3% ; ; 3% ; ; 7.5%; Baird-Parker ; ; ; ; ; 10% ; LB1; LB2; ; ; 仪器 BAX Q7 ; VITEK COMPACT2 ; VIDAS 方法 [4-11],, : ; ; ; ; ; ; ; 3 结果与分析 3.1 各致病菌的检出率 150, 8, 2 表 1 采样品种及数量 Talbe 1 The number and species of sampling / (( ) / ( ) / ( ) / 鮃鮃

3 表 2 检测结果统计表 Table 2 The statistic results of detection (%) ( ) () () , (), 100%; 141, 9, 94.0%; 149, 1, 99.3%; ( )140, 10, 93.3%; ( )1, 1, 50.0%,,,, 3.2 不同品种的合格率 40, 4 10,,, 7, 3 3,,,, 72.2%,,,,, BAX Q7, VITEK COMPACT2, ( ) 3.3 不同规模的餐饮企业样品的检验结果 26, 17%; 23, 15%; 57, 38%; 32, 21%; 12, 8%, 4 4, 92%, 91%, 84% 81% 83%,,,,, ;,,,, Table 3 表 3 不同品种的样品合格率统计表 The statistic results of qualified rates from different species of samples () (%) 19(2) (2) (5) (1) (1) (1)

4 4, : 1437 Table 4 表 4 不同规模的餐饮企业样品的合格率统计表 The statistic results of qualified rates from the samples of different sizes of the catering enterprises () (%) 26(2) (2) (9) (6) (2) 近海远海及淡水样品的检验结果 100, 67%; 38, 25%; 12, 8%, , 92.1%; 12 1, 91.7%; 100, 9, 6, 1, 1, 83.0%,,,,,,, GB ,, BP,,,,,,,,,,,, 3.5 甲壳类软体动物类及鱼类水产品的检验结果 64, 43%; 15, 10%; 71, 47%, 6 6,,, 81.2%;,, 88.7%;,, 1, 93.3%,, 3.6 分析,,,,, (), ( )( ),,,,,,,,,,, [3,12] [13],,

5 Table 5 表 5 近海远海及淡水样品各个项目合格率结果 The statistic results of qualified rates from the samples of offshore, far from the sea and fresh water () (%) 12(1) (17) (3) Table 6 表 6 甲壳类软体动物类及鱼类水产品检验结果 The statistic results of qualified rates from the samples of crustaceans, pelecypoda and fish aquatic products () (%) 64(12) (1) (8) , , 86% 82%, 86%, 92% 6% ; 1,, 0.6%;, 0%; 6%; 12%; ( ) 2, 1, 50%,,, : 2008~ % [14], % [15], 2009~ % [16], 2011~ %, 1.2% [17],,,,,,, 参考文献 [1] Heinitz ML, Ruble RD, Wagner DE, et al. Incidence of salmonella in fish and seafood [J]. J Food Rort, 2000, 63: [2],,,. [J]., 2006, 22(4): Dai CF, Fang YY, Yan JW, et al. Evaluation of contaminative status and safety of prevalent pathogenic organisms in edible raw aquatic products [J]. China J Public Health, 2006, 22(4): [3],,, [J]., 2011, 6: Lin TH, Zu WJ, Gou Q, et al. Evaluation of contaminative status and safety of food-borne pathogens in edible raw aquatic products in Guangdong in 2010 [J]. Travel Med Sci, 2011, 6: [4] GB [S]. GB Food Microbiological Examination:Salmonella [S]. [5] GB [S] GB Food Microbiological Examination:Listeria monocytogenes [S]. [6] GB/T [S]. GB/T Microbiological examination of food hygiene-examination of Vibrio parahaemolyticus [S]. [7] GB [S]. GB Food Microbiological Examination:Shigella [S]. [8] GB [S] GB Food Microbiological Examination: Staphylococcus aureus [S].

6 4, : 1439 [9] GB [S]. GB Food Microbiological Examination:Aerobic plate count [S]. [10] GB/T [S]. GB/T Microbiological Examination of food hygiene-detection of Coliform bacteria [S]. [11] GB [S]. GB Hygienic standard for salt liquor-saturated aquatic products of animal origin [S]. [12]. [J]., 2010, 4: 83. Ren CM. Microbiology of edible raw aquatic products can be controlled by vinegar [J]. Health world, 2010, 4: 83. [13],,. [J]., 2011, 7: Xiao J, Liang ZA, Lin XM. Effects of frozen storage on microorganism in the high-risk foods [J]. Food Ind, 2011, 7: [14],,, [J]., 2011, 27(5): Wang MY, Qiao X, Fu XM, et al. Food-borne pathogens in meat and aquatic products in Jiangsu province, [J]. China J Public Health, 2011, 27(5): [15],, [J]., 2013, 25(4): Song XH, Qiao M, Yiu Y. Surveillance on foodborne pathogens in foods in Shanxi province in 2010 [J]. Chin J Food Hyg, 2013, 25(4): [16],,, [J],, 2015, 31(1): 7 9 Hou J, Chen YF, Luan MC, et al. Investigation of pollution condition on foodborne pathogens in food from Dalian city during [J]. J Med Pest Control, 2015, 31(1): 7 9 [17],,, [J]., 2014, 26(4): Chen J, Gu QF, Liu C, et al. Monitoring results on food-borne pathogens in food in Shanghai from 2011 to 2012 [J]. Shanghai J Prev Med, 2014, 26(4): 作者简介 ( 责任编辑 : 白洪健 ) 赵丽元, 硕士, 主管药师, 主要研究方向为微生物检验 [email protected]

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Microsoft Word % 8 1 Vol. 8 No. 1 2017 1 Journal of Food Safety and Quality Jan., 2017 2015 孙寒松 *, 王德宇 (, 132001) 摘要 : 目的, 方法 2014 结果 2015 10 240, 19, 7.92%, 7, 8, 3, 1 ; 3 : 57.14%, 50%, 2%; 结论,, 关键词 : ; ; Analysis of