Microsoft Word _二校

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1 7 9 Vol. 7 No Journal of Food Safety and Quality Sep., 2016 李兵兵, 刘纯成, 侯海燕, 李双姝, 刘 * 靓 (, ) 摘要 : 目的, 方法, 70 ( ) 40 ( ) O157,, 结果 41.43%, 22.50%, ; / 噁结论, 关键词 : ; ; ; Distributing disciplinarian and drug resistance of pathogenic bacteria in Procambarus clarkii and its external environment in Huai an LI Bing-Bing, LIU Chun-Cheng, HOU Hai-Yan, LI Shuang-Shu, LIU Liang * (Huai an Center for Disease Control and Prevention, Huai an , China) ABSTRACT: Objective To analyzed the distributing disciplinarian and drug resistance of pathogenic bacteria in Procambarus clarkii and its external environment in Huai an, so as to provide basis for prevention and control of foodborne disease. Methods The Salmonella, Staphylococcus aureus, Listeria monocytogenes, Escherichia coli O157 and Vibrio parahaemolyticus in 70 samples of Procambarus clarkii and 40 samples of external environmental water were tested according to the national standards of food microbiological examination. Then the serotype distribution of Salmonella were identified, and the drug resistances of Salmonella and Staphylococcus aureus were determined by Kindy-Bauer (KB) method. Results The total detection rate of pathogenic bacteria in Procambarus clarkii was 41.43%. The pathogenic bacteria included Salmonella, Staphylococcus aureus, Vibrio parahaemolyticus and Listeria monocytogenes. The total detection rate of pathogenic bacteria in external environmental water was 基金项目 : Fund: Supported by the Soft Subject of Natural Science in Huai an City. * 通讯作者 :,, @qq.com *Corresponding author: LIU Liang, Supervising Technician, Huai an Center for Disease Control and Prevention, Huai an , China @qq.com

2 9, : %, and the pathogenic bacteria included Staphylococcus aureus, Salmonella and Vibrio parahaemolyticus. Salmonella isolates showed high resistance to nalidixic acid and ampicillin, and Staphylococcus aureus isolates showed high resistance to erythromycin, trimethoprim/sulfamethoxazole and clindamycin. Conclusion There is pollution of pathogenic bacteria in Procambarus clarkii and its external environment water in Huai an, and the drug resistance of Salmonella and Staphylococcus aureus was serious. KEY WORDS: Procambarus clarkii; external environment water; pathogenic bacteria; drug resistance 1 引言,,,,,,, [1],,, [2,3], 2001~ , , 1427,,, 2055, 40.93% [4],,, 2 材料与方法 2.1 样品采集 70 ( ) 40 ( ) 2 h 2.2 仪器与试剂 VITEK Compact II ( ); PYX-DHS (); BHC-1300A/B2 ( ) (TTB) (SC) 7.5%LB EC, 3% MH ; O157 ; ; GP GN Biomerieux ; : (CTX)(CAZ) (TET) (CFX)(GEN)(AMP) (CIP) / 噁 (TMP/SMZ) (NAL) (CHL)(DA) (E) (OX)(CIP) 2.3 实验方法致病菌的分离与鉴定 5 :, GB [5] ;, GB [6] ;, GB [7] ; O157, GB/T O157: H7/NM [8] ;, GB [9] GB [5] 药敏实验 Kirby-Bauer [10], ATCC25922 ATCC25923, (Clinical and Laboratory Standards Institute, CLSI) [11] 3 结果与分析 3.1 致病菌的分离与鉴定致病菌检出情况, 29, 41.43%,, 26.67%, (20.00%) (10.00%)

3 (2.86%), O157 ;, (30.00%) (25.00%) (15.00%), O157, 22.50% 13.33%, 6.67%, O157; (8.00%)(4.00%) 沙门氏菌的血清型鉴定 23, 2 23 B D E1 E4 4,,, (8 )(4 ) (2 ) (2 ) (1 ) (3 )(1 ) (1 ) (1 ) 3.2 药敏实验结果沙门氏菌的耐药性 1, 10, (86.96%) (78.26%)(43.48%) (34.78%) / 噁 (34.78%)(21.74%) (17.39%)(17.39%) (13.04%) (8.70%), 23, 金黄色葡萄球菌的耐药性, (95.00%), / 噁 (75.00%) (65.00%)(25.00%)(15.00%) (15.00%), ( 2) 3 2, 9 3, 4 4 Table 1 表 1 致病菌检出情况 Detection results of pathogenic bacteria / / / / O157 / / 表 2 沙门氏菌的血清型分布 Table 2 Serotype distribution of Salmonella / % (S. typhimurium) B (S. enteritidis) D (S. anatum) E (S. london) E (S. senftenberg) E

4 9, : Fig. 1 Drug resistance of Salmonella : (CTX); (CAZ); (TET); (CFX); (GEN); (AMP); (CHL); (CIP); / 噁 (TMP/SMZ); (NAL),,,,, [17,18],,, / 噁 3 [19] 56.52%, 65%, [20],,,,,, 参考文献 [1],,,. [J]., 2015, 35(2): CHEN YN, WANG YW, WEI YS, et al. Evolution and standard comparison of indicator microorganisms for different surface waters [J]. Acta Sci Circum, 2015, 35(2): [2] 虓. [J]., 2014, 14(3): Fig. 2 Drug resistance of Staphylococcus aureus : (GEN); (CHL); (DA); / 噁 (TMP/SMZ); (E); (TET); (CFX); (OX); (CIP) 4 讨论与结论, 41.43%, [12],, [13] 4,, [14-16],, ;,, 22.50%,, ZHOU H. Contamination status of foodborne pathogens in meat and aquatic products in China [J]. J Trop Med, 2014, 14(3): [3]. [J]., 2012, 18(2): HUANG ZY. Epidemic and monitoring status of foodborne diseases [J]. Appl Prev Med, 2012, 18(02): [4], [J]., 2012, 28(27): Xu JF, Zhang JZ. Analysis of foodborne disease outbreaks in China from 2001 to 2010 [J]. Chin Agric Sci Bull, 2012, 28(27): [5] GB [S]. GB National food safety standard Food microbiological examination: Salmonella [S]. [6] GB [S]. GB National food safety standard Food microbiological examination: Staphylococcus aureus [S]. [7] GB [S]. GB National food safety standard Food microbiological examination: Listeria monocytogenes [S].

3534 7 [8] GB/T 4789.36-2008 O157: H7/NM [S]. GB/T 4789.36-2008 National food safety standard Microbiological examination of food hygiene Examination of Escherichia coli O 157: H7/NM [S]. [9] GB 4789.

The prevalence of antimicrobial resistance and carriage of virulence genes in Staphylococus aureus isolated from food handlers in Kuwait City restaurants [J]. J Clin Microbiol, 2006, 44(6): 2119 2125.

5 [8] GB/T O157: H7/NM [S]. GB/T National food safety standard Microbiological examination of food hygiene Examination of Escherichia coli O 157: H7/NM [S]. [9] GB [S]. GB National food safety standard Food microbiological examination: Vibrio parahaemolyticus [S]. [10] Udo EE, Al-Mufti S, John AM. The prevalence of antimicrobial resistance and carriage of virulence genes in Staphylococus aureus isolated from food handlers in Kuwait City restaurants [J]. J Clin Microbiol, 2006, 44(6): [11] Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing; twenty informational supplement [S] [12],,, [J]., 2011, 27(3): WANG YM, QIAO X, FU XM, et al. Food-borne pathogens in meat and aquatic products in Jiangsu province, [J]. Chin J Public Health, 2011, 27(3): [13],,,. [J]., 2013, 33(4): LI YY, HE JY, ZHANG WW, et al. Analysis on foodborne pathogenic bacteria contamination in retailed aquatic products in Guangzhou [J]. Chin f Food Hyg, 2013, 33(4): [14],, [J]., 2015, 42(5): SU CL, SHEN L, YANG L. Analysis on detection of food-borne pathogens in Chengdu, 2012 [J]. Mod Prev Med, 2015, 42(5): [15] Newton A, Kendall M, Vugia DJ, et al. Increasing rate of vibriosis in the [17],,,. [J]., 2010, 50(6): YANG BW, QU D, SHEN JL, et al. Antimicrobial susceptibility and related genes of Salmonella serovars from retail food in Shaanxi Province [J]. Acta Microb Sin, 2010, 50(6): [18],,,. [J]., 2013, 23(15): MA N, SUN W, ZHENG H, et al. Analysis of serotype distribution and drug resistance of foodborne Salmonella in Liaoning Province [J]. Chin J Health Lab Technol, 2013, 23(15): [19],,,. [J]., 2012, 28(11): XU BJ, ZHANG WS, WANG X, et al. Toxin genes detection and antimicrobial susceptibility test of Staphylococcus aureus isolated from retail chicken in Shaanxi Province [J]. Chin J Zoono, 2012, 28(11): [20],,, [J]., 2012, 24(1): CHEN YZ, SHAO K, GUAN B, et al. Ananalysis on the serotypes and antibiotic sensitivities of foodborne Salmonella in Shandong Province from 2003 to 2010 [J]. Chin J Food Hyg, 2012, 24(1): 作者简介 ( 责任编辑 : 刘丹 ) 李兵兵, 硕士, 助理研究员, 主要研究方向为微生物学检验 leeb1002@163.com United States, : review of surveillance date from 2 systems [J]. Clin Infect Dis, 2012, 54(Suppl 5): S [16],,,. [J]., 2015, 27(5): LV GP, LI LJ, LU F, et al. Molecular typing of foodborne Staphylococcus 刘靓, 硕士, 主管技师, 主要研究方向为微生物学检验 @qq.com aureus by pulsed-field gel electrophoresis [J]. J Microb, 2015, 27(5):

Microsoft Word 苟伟民_二校

Microsoft Word 苟伟民_二校 8 1 Vol. 8 No. 1 2017 1 Journal of Food Safety and Quality Jan., 2017 2011~2015 苟伟民 *, 徐雪梅 (, 133000) 摘要 : 目的 2011~2015,, 方法 2013 2011~2015 1069 结果 1069 115, 10.76% 17.65% (3/17) 15.79% (3/19) 11.76% (4/34)