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1 8 1 Vol. 8 No Journal of Food Safety and Quality Jan., ~2015 赵薇 *, 杨修军, 刘桂华, 黄鑫, 王慧, 张炜煜, 石奔 (, ) 摘要 : 目的, 方法 2011~ , GB 结果 258, 3.15% , 5.69% 4.68% 4.42%, 6.54% 5.77% 3.47% 结论, 关键词 : ; ; ; Analysis on the monitoring data of Staphylococcus aureus in food of Jilin province in 2011~2015 ZHAO Wei *, YANG Xiu-Jun, LIU Gui-Hua, HUANG Xin, WANG Hui, ZHANG Wei-Yu, SHI Ben (Jilin Provincial Center for Disease Control and Prevention, Changchun , China) ABSTRACT: Objective To investigate the status of market food contaminated with Staphylococcus aureus (S.A.) in Jilin province, so as to provide scientific research data for the prevention and control of foodborne disease. Methods According to GB National food safety standard-food microbiological examination: Staphylococcus aureus, S.A. of 8203 samples collected from 17 kinds of foods in 9 regions of Jilin province in 2011~2015 were detected. Results Two hundred and fifty-eight strains of S.A. were isolated and the total positive rate was 3.15%. The positive rates of 2014 and 2015 were increased significantly. The positive rates of Tonghua, Baishan and Siping cities were 5.69%, 4.68% and 4.42%, which were much higher than other regions. By comparison, the positive rates of meat and meat products, milk and dairy products and eggs and egg products were higher than others, which were 6.54%, 5.77% and 3.47%, respectively. There was no significant difference between the positive rate of catering and service industry and that of circulation link. The contamination rate of bulk food was significantly higher than that of pre-packaged food. Conclusion The market foods contaminated with Staphylococcus aureus 基金项目 : (2014Q012) Fund: Supported by the Youth Scientific Research Program of Jilin Provincial Health and Family Planning Commission Project (2014Q012) * 通讯作者 :,, weizhao81226@126.com *Corresponding author: ZHAO Wei, Associate Chief Technician, Jilin Provincial Center for Disease Control and Prevention, Changchun , China. weizhao81226@126.com

2 1, : 2011~ show varying degrees in all regions of Jilin province. The supervision and regulation of the food production with high risk of contamination should be strengthen. KEY WORDS: Staphylococcus aureus; foodborne disease; Jilin province; risk monitoring 1 引言 (Staphylococcus aureus, S.A.) ( ), 30%~80% [1],, [2,3], [4],,, 2 材料与方法 2.1 样品来源 9 ( ), 2011~2015,, 培养基及试剂 ( ); Baird-Parker ( ); VITEKⅡ ( ) 2.3 检测指标及方法 GB [5], (most probable number, MPN) 2.4 菌株的初检与复核 Baird-Parker,, VITEK Ⅱ, 2.5 数据统计分析 Excel 2007, SPSS17.0 X, P< 结果与分析 ~2015 年间吉林省食品监测中金葡菌污染检测结果 2011~ , 258, 3.15%, 2014 ; , 表 1 不同年份及采样地区的食品中金黄色葡萄球菌监测结果 Table 1 Monitoring results of S.A. from foods in different years and regions /% %(64/1124) 2.21%(3/136) 2.40%(3/125) 4.00%(17/425) 9.04%(15/166) 9.56%(26/272) 4.68%(45/961) 4.41%(6/136) 3.62%(5/138) 5.37%(11/205) 4.14%(7/169) 5.11%(16/313) 4.42%(34/769) 7.35%(10/136) 2.17%(3/138) 6.83%(14/205) 5.31%(6/113) 0.56%(1/177) 3.46%(24/693) 0.74%(1/136) 2.17%(3/138) 2.44%(5/205) 7.96%(9/113) 5.94%(6/101) 2.80%(29/1035) 6.62%(9/136) 2.90%(4/138) 1.18%(5/425) 7.08%(8/113) 1.35%(3/223) 2.71%(31/1142) 4.20%(12/286) 0.00%(0/125) 0.24%(1/425) 7.08%(8/113) 5.18%(10/193) 1.95%(13/666) 2.94%(4/136) 1.45%(2/138) 0.00%(0/205) 2.65%(3/113) 5.41%(4/74) 1.31%(11/841) 2.21%(3/136) 0.00%(0/138) 0.55%(2/365) 1.77%(2/113) 4.49%(4/89) 0.72%(7/972) 0.00%(0/136) 2.17%(3/138) 0.00%(0/405) 0.88%(1/113) 1.67%(3/180) 3.15%(258/8203) 3.49%(48/1374) c 1.89%(23/1216) a 1.92%(55/2865) 5.24%(59/1126) 4.50%(73/1622) b : a: (X 2 = , P 0.05), 2015 ( X 2 =0.7941, P>0.05) b: ( X 2 = , P 0.05), 2011 ( X 2 =1.9470, P>0.05) c: ( X 2 = , P 0.05)

3 不同采样地区的食品中金葡菌监测结果 ( ), 2015, 2014, % 9, ( X , P<0.05), 不同类别食品金黄色葡萄球菌监测结果 5 17, : 11,, 3, X , P> _ _ ( ) 不同采样地点类型食品中金黄色葡萄球菌监测结果 ,, 3.44% 3.04%, ( X 2 =0.8889, P>0.05),, _ _,, / 6 ( X 2 =6.0791, P>0.05) /, ;,,,, /, / / /, 5 ( X 2 = , P>0.05) 3 表 2 不同种类食品中金黄色葡萄球菌监测结果 Table 2 Monitoring results of S.A. from different kinds of foods % % ( ) e % f % % % % % % % % % % % % _ _ % _ _ dg % _ % % % % %

4 1, : 2011~ 续表 2 % % % % _ % _ % % % _ % _ % _ % _ % _ % % % % % % % % % : d: ( X , P<0.05) e: ( ) ( X , P 0.05) f: ( X , P 0.05) g: ( X , P 0.05) Table 3 表 3 不同采样地点食品中金葡菌监测结果 Monitoring results of S.A. from foods collected from different collecting locations % % _ / % _ / _ % _ / _ % _ / _ % _ / _ % _ % _ _ _ % _ _ _ %

5 102 8 续表 3 % _ _ _ % _ _ _ % _ _ _ % _ ( ) % _ % _ % _ % % _ % _ _ ( ) % _ _ % _ _ ( ) % _ _ / % _ _ % _ _ / % _ _ % _ _ % _ _ % % % 3.5 不同包装类型食品中金黄色葡萄球菌监测结果 : ( ), 3.47% (181/5215),, 2.58% (77/2988), ( X 2 =4.9815, P<0.05) 4 讨论 4.1 与其他省份监测结果的比较 2011~2015,, % [6] 2010~ , 0.72%(11/1520) 2011, ( X 2 = , P<0.05) [7] , ( X 2 =1.1960, P>0.05) [8], %(25/1380), %(20/1100), %(18/1850) [9] 2011~ ~2015 ( X 2 =1.6420, P>0.05), , 2015,, 2014,,, 3, %, 4.2 不同类别食品金黄色葡萄球菌监测结果分析 17,, 3,,, 13.06%,

6 1, : 2011~ ( ) 16.77%, [10,11] [12] [13],,,,, 4.3 不同采样地点类型食品中金黄色葡萄球菌监测结果分析,,,,,, /,,,,, /,, 3, [14],,,, [15],,, 5 结论, 2011~2015,, 2 9,,,,,,, 参考文献 [1] Kluymans J, Van Belkum A, Verbrugh H. Nasal carriage of Staphylococcus aureus: epidemiology, underlying mechanisms, and associated risks [J]. Clin Microbiol Rev, 1997, 10(3): [2],,,. [J]., 2008, 34(12): Li WJ, Huang H, Fu HR, et a1. Growth model of Staphylococcus aureus in simulated crab meat [J]. Food Ferm Ind, 2008, 34(12): [3],,. [J]., 2008, 29(8): Xu XX, Yan J, Zhen Z. Establishment of growth model of Staphylococcus aureus in raw milk [J]. Sci Technol Food Ind, 2008, 29(8): [4],,,. [J]., 2016, 32(8): Liu YH, Liu YF, Yuan J, et al. Molecular epidemiology study of food poisoning caused by Vibrio parahaemolyticus and Staphylococcus aureus [J]. J Med Pest Control, 2016, 32(8): [5] GB [S]. GB National food safety standard-food microbiological examination: Staphylococcus aureus [S]. [6],,, [J]., 2012, 28(1): Ma N, Zhao H, Zhang X, et al. Survey on foodborne pathogens in Liaoning province in 2010~2011 [J]. Chin J Public Health Manag, 2012, 28(1): [7],,, [J]., 2012, 22(12): Chui HX, Zhang D, Zhang XL, et al. Monitoring and analysis for food-borne pathogens in Henan province in 2011 [J]. Chin J Health Lab Technol, 2012, 22(12): [8],,,. 2006~2013 [J]., 2016, 32(1): Wei Q, Shen M, Liu X, et al. Pathogenic bacteria monitoring results analysis in food from Ningxia area during 2006~2013 [J]. J Med Pest Control, 2016, 32(1): [9], 璟, [J]., 2016, 31(2): Li JB, Zhang J, Hu XN. Investigation on contamination of Staphylococcus aureus in instant food in Jiayuguan from 2011 to 2014 [J]. Bull Dis Control Prev, 2016, 31(2): [10],,,. [J]., 2009, 19(1): Zhang LY, Li Y, Chen HY, et al. Cooked food products microbial contamination survey in Wenzhou [J]. Chin J Health Lab Technol, 2009, 19(1): [11],,,. [J]., 2012, 30(5): Xu ZW, Han YY, Meng J, et al. Basic research on risk assessment of Staphylococcus aureus in meat products [J]. Packag Food Mach, 2012, 30(5): [12],,,. [J]., 2016, 26(5): Zhang SY, Jiang X, Wang XH, et al. Detection and analysis of

7 104 8 Staphylococcus aureus and Salmonella in Longwan district of Wenzhou [J]. Chin J Health Lab Technol, 2016, 26(5): [13],,,. 757 [J]., 2015, 42(23): Wang YM, Tang Z, Qiao X,et al. Investigation on food-borne pathogens in 757 samples of student meal Jiangsu province [J]. Mod Prev Med, 2015, 42(23): [14],,, [J]., 2012, 22(11): Guo F, Huang XM, Peng B, et al.analysis of contamination status of food-borne pathogens in Suizhou from 2009 to 2011 [J]. Chin J Health Lab Technol, 2012, 22(11): [15],,. [J]., 2016, 32(3): Chen HZ, Yang N, Liu B. Contamination status of main foodborne pathogens in food in Shengyang city [J]. Occup Health, 2016, 32(3): 作者简介 ( 责任编辑 : 姚菲 ) 赵薇, 副主任技师, 主要研究方向为微生物检验 weizhao81226@126.com 食品掺假与食物中毒专题 征稿函,,,,,,,,, (1) ; (2) ; (2) ; (3), ,,, 投稿方式 : : jfoodsq@126.com 食品安全质量检测学报 编辑部