蛋白分析领域的“重磅炸弹”—介绍免费蛋白序列分析软件包ANTHEPROT 4

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裙蕊顾井
9 years ago
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1 HELP doc_ant.exe HTML 10, :

2 ANTHEPROT 4.3 ANTHEPROT 4.3 Institute of Biology and Chemistry of Proteins Unix DOS WINDOWS95 98 WINDOWS Anthe4_3c.exe 1.9 Anthewin ANTHEPROT 4.3

3 ANTHEPROT 4.3 File/Open *.SEQ ANTHEPROT 3.3 DNA/Strider EMBL NBRF Pearson/Fasta PIR IG/Stanford ); *.BAS ( Pearson/Fasta 30 32K ); ClustalV ClustalW *.ALN ClustalW Multalin 4.1 *.MUL Prosite Site *.SIT PDB *.PDB IBCP *.CNS ANTHEPROT *.SEQ Pearson/Fasta >

4 >MBN VLSEGEWQLVLHVWAKVEADVAGHGQDILIRLFKSHPETLEKFDRFKHLKTEAEMKASEDL KKHGVTVLTALGAILKKKGHHEAELKPLAQSHATKHKIPIKYLEFISEAIIHVLHSRHPGD FGADAQGAMNKALELFRKDIAAKYKELGYQG Undo Ctrl+Z Ctrl+T

5 Methods

6 PROSITES Internet Helical Wheel Dot Plot 1. Dot Plot Dot Plot 2

7 Dot Plot Dot Plot Dot Plot

8 2. Similarity Search Methods/Similarity Search/WWW or Local Fasta, Methods/Similarity Search/WWW-Blast at PBIL server SwissProt 3. Site/signature ANTHEPROT Methods / Site detection (Prosite) PROSITE 100% Mismatch 1 or 2 Similarity level PROSITE PROSITE.DAT PROSITE.DOC ANTHEPROT

9 PROSITE 4. Pattern search Databases/Pattern search PROSITE - - [ ] - { } - x - x(3) 3

10 N-[PT]-{GM}-x(2)-[ILVM] N-P-K-G-H-V, N-T-L-K-G-M N-L-K-G-H-V, N-T-G-K-H-V Pearson SEQ_x.BAS 5. Antheprot 115 L 5 AIIHVLHSRHP -7 5 Shift+ Hydrophobicity profile

11 Hydrophilicity profile Solvent accessibility profile Antigenicity profile transmembranous regions profile Combined antigenicity profile 6. Antheprot GOR I Method/Secondary structure prediction/garnier et al Gibrat Method/Secondary structure prediction/gibrat et al Double Prediction Method DPM Method/Secondary structure prediction/dpm Homologue Method/Secondary structure prediction/levin Predator Method/Secondary structure prediction/ Predator Method/Secondary structure prediction/all Ctrl+L

12 GOR I Gibrat Double Prediction Method DPM

13 Homologue Predator X *.XRA Pearson/Fasta

14 *.xra >Myoglobin CCCHHHHHHHHHHHHHHTTSHHHHHHHHHHHHHHHCHHHHHTCHHHHTCCSHH HHHHCHHHHHHH HHHHHHHHHHHTTTTCCHHHHHHHHHHHHHTTCCCHHHHHHHHHHHHHHHHHH CTTTTSHHHHHH HHHHHHHHHHHHHHHHHHHTCCC H E T C 7. Methods/AA Composition, specific volume, M. Weight Ctrl+M

15 8. ANTHEPRO4.3 Methods/Titration curve Ctrl+U

16 9. Helical Wheel ANTHEPRO4.3 Helical Wheel Methods/ Helical Wheel Helical Wheel Helical Wheel

17 10. ANTHEPRO4.3 Methods/Potential cleavage site of signal pept.

18 ANTHEPRO4.3 WORD *.BMP Invert Mode Options

19 ANTHEPRO4.3 ANTHEPRO4.3 ANTHEPRO4.3 ANTHEPRO4.3 ftp://ftp.ibcp.fr/pub/antheprot/windows

蛋白分析领域的“重磅炸弹”—介绍免费蛋白序列分析软件包ANTHEPROT 4

蛋白分析领域的“重磅炸弹”—介绍免费蛋白序列分析软件包ANTHEPROT 4 Antheprot 中文使用说明书作者 : 谈杰一前言... 1 二软件使用详解... 1 1 序列编辑功能... 2 2 工具栏与基本功能... 3 3 基本分析工具介绍... 5 三结果的输出以及与其它应用程序进行数据交换... 14 四结束语... 14 一前言生物的化学, 就是蛋白质与核酸的化学对蛋白质的研究是生物化学领域一个非常重要的部分近年来, 通过实施基因组计划,