Microsoft Word 戴莹-二校出425

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万叶赫那拉
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1 7 2 Vol. 7 No Journal of Food Safety and Quality Feb., * 戴莹, 王纪华, 韩平, 马帅, 冯晓元 (, ( ),, ) 摘要 :,,,, 2, 4-6,, 关键词 : ; ; Research progress of 6 kinds of plant growth regulator residue limits standards and detection methods in agricultural products DAI Ying, WANG Ji-Hua, HAN Ping, MA Shuai, FENG Xiao-Yuan * (Beijing Research Center for Agricultural Standards and Testing, Risk Assessment Lab for Agro-products (Beijing), Ministry of Agriculture, Beijing Municipal Key Laboratory of Agriculture Environment Monitoring, Beijing , China) ABSTRACT: Plant growth regulators (PGRs) is a class of synthetic pesticides which can be used to improve crop quality and regulate their growth. In recent years, the abuse and misuse of regulators caused increasing food safety problems had aroused extensive concern in China. This paper summarized the advances of forchlorfenuron, gibberellin, paclobutrazol, ethephon, chlormequat and 2, 4-dichlorophenoxyacetic acid, which are the most widely used PGRs in agricultural products, including the maximum residue limits and the detection technologies of PGRs. With the continuous development and perfection of novel technology, the analysis technology of PGRs is being developed with a tendency of multi-residuals, high efficiency, high specificity and high sensitivity. KEY WORDS: plant growth regulators; maximum residue limits; detection 基金项目 : (QNJJ201521) ( ) ( ) ( ) (ATFM-KFKT ) Fund: Supported by Youth Foundation of Beijing Academy of Agriculture and Forestry Sciences (QNJJ201521), Special Fund for Agro-scientific Research in the Public Interest ( ) and the Open Project of Beijing Research Center for Agricultural Standards and Testing of Chinese Ministry of Agriculture (ATFM-KFKT ) * 通讯作者 :,,, fengxiaoyuan2014@126.com *Corresponding author: FENG Xiao-Yuan, Professor, Beijing Research Center for Agricultural Standards and Testing, Room 703, Block A, Beijing Agricultural Building, No.11 Middle Road of Shuguang Garden, Haidian District, Beijing , China. fengxiaoyuan2014@126.com

2 引言,, : 2, 4- (2,4-Dichlorophenoxyacetic acid, 2, 4-D),,, [1,2], 200 mg/l,,,, [3] Tuluce [4] [5] 鮋 50 d, 鮋, - Chen [6] 128 mg/kg mg/kg,,, [7-9],, [10] [11], LD mg/kg, ; LD mg/kg, 2, 4-D,, [11] 2,4-D [12], (acceptable daily intake, ADI),, [13,14],, (Codex Alimentarius Commission, CAC) (maximum residue limits, MRLs),,,, 6 2, 4-D MRLs 2 植物生长调节剂的限量标准和检测方法 2.1 氯吡脲 (forchlorfenuron),,,,,, CAC MRLs, 0.01~0.06 mg/kg, 41 ( ) MRLs, 0.01 mg/kg, 0.1 mg/kg 400 MRLs, 0.01~0.05 mg/kg 7 MRLs, MRLs 0.05~0.1 mg/kg [15-19] 1 表 1 常见农产品中氯吡脲最大残留限量值 (mg/kg) Table 1 MRLs of forchlorfenuron in agricultural products (mg/kg) / / 0.1 / / / / 0.1 / / 0.1 / / 0.01 / 0.1 / / 0.1 / / / / / / / / 0.01 / 0.1 / / 0.01 / 0.1 / / / : / [20] [21,22] [23,24], - (liquid chromatography-ultraviolet, LC-UV) - (liquid chromatography-mass spectrometry,

3 2, : LC-MS) [25-27] SPE PSA, LC-UV, μg/kg, LC-MS, MRM,,, Valerde [28] QuEChERS(quick, easy, cheap, effective, rugged and safe),,,,, LC-MS,, 1 μg/kg, 10 μg/kg Campillo [29], LC-ESI-TOF, 0.02~0.05 ng/g, 89%~106%, 0.2~1.0 ng/g 2.2 赤霉素 (gibberellin), 38, (gibberellic acid 3, GA 3 ),,,,,,, GA 3 50, 30, MRLs, 0.2 mg/kg [19] CAC GA 3 GA 3 LC, Xie [30] LC-MS/MS GA 3, 10 μg/kg, 77.8%~96.2% [31] 0.5%(V:V) (V:V=4:1), Strata-X, LC-MS/MS, GA 3 2.0~100.0 μg/l, 0.999, 0.3 μg/l, 1.0 μg/l, 90.3%~94.2% Li [32], LC-FLD, 2.3 多效唑 (paclobutrazol),,,,,, 7 0.2~0.5 mg/kg [16] 378 MRLs, MRLs 0.05 mg/kg MRLs 0.5 mg/kg, MRLs 0.02 mg/kg [18] 27 MRLs, 0.01~1 mg/kg, MRL 0.05 mg/kg, MRLs 0.01 mg/kg, MRLs 0.5 mg/kg, MRLs 0.2 mg/kg [19] CAC MRLs [15,17] 2 表 2 常见农产品中多效唑最大残留限量值 (mg/kg) Table 2 MRLs of paclobutrazol in agricultural products(mg/kg) / / 0.05 / / / / / / / 0.5 / / / / / / / / 0.5 / / 0.01 / / 0.5 / 0.01 / / 0.5 / 1 / / 0.5 / 0.01 / / 0.5 / 0.2 / (gas chromatography, GC) (GC-MS) (LC-MS) GC 法 [33] GC,,, GC, 72.16%~103.6%, 0.02 mg/kg [34] GC,,, GC, 85%~105%, 1.2 μg/kg GC-MS 法 [35] - (GC-MS/MS), 0.02~5.00 mg/kg,

4 mg/kg, 82.82%~101.35% [36] GC-MS/MS, 86.7%~126.7%, μg/kg LC-MS 法 Ferracini [37] LC-MS/MS, 0.25 μg/kg, 1.25 μg/kg, 82%~94% [38] - (UPLC-MS/MS),,, 85.4%~99.4%, 84.3%~99.3%, 0.5 μg/kg 2.4 矮壮素 (chlormequat),,,, CAC MRLs, CAC 24 MRL, 0.04~30 mg/kg [15] 11 MRLs 0.1~10 mg/kg [16] 300, 0.05 mg/kg, MRLs 0.1 mg/kg, MRLs 7 mg/kg, MRLs 0.1 mg/kg [18] 100 MRLs, MRLs 3 mg/kg, MRLs 2 mg/kg, MRLs 1 mg/kg, 0.05 mg/kg, MRLs 0.05 mg/kg, MRLs 0.05~10 mg/kg MRLs 0.05 mg/kg, MRLs 10 mg/kg, MRLs 0.05 mg/kg, MRLs 3 mg/kg, MRLs 0.1 mg/kg [19] MRLs [17] 3,,,,,, [39] LC-MS, ( : 122/58 m/z 122/63 m/z),, [40] [41] Strata-X-C, HPLC-MS/MS, 0.02~10 μg/l, 0.05~0.07 μg/kg, 0.02~0.03 μg/kg Guo [42] HPLC-MS, 0.1 mg/kg 表 3 常见农产品中矮壮素最大残留限量值 (mg/kg) Table 3 MRLs of chlormequat in agricultural products(mg/kg) / / / 0.05 / / 0.05 / / 0.5 / 0.05 / / 0.05 / / 0.05 / / / / / 5 2 / / / / 0.05 / / / / / / / / : / 2.5 2, 4-D 2, 4- (2,4-dichlorophenoxyacetic acid, 2, 4-D),, 2, 4-D,,,, CAC 2, 4-D MRLs CAC 25 ( ) 2, 4-D MRLs,, 2,4-D MRLs0.01~2 mg/kg [15] GB / 6 2, 4-D MRLs, 2 mg/kg, 0.5 mg/kg, 0.01~0.2 mg/kg [16] 378 2, 4-D MRLs, 2, 4-D

5 2, : mg/kg, 2, 4-D MRLs 1 mg/kg, 2, 4-D MRLs 0.05 mg/kg, 2, 4-D MRLs 0.1 mg/kg [17] 131 2, 4-D MRL, 33 2, 4-D MRLs 0.01~5 mg/kg, 2, 4-D MRLs 2 mg/kg, MRLs 5 mg/kg, 2, 4-D MRLs 0.05~0.08 mg/kg, 2, 4-D MRLs 0.2 mg/kg, 2, 4-D MRLs 5 mg/kg, 2, 4-D MRLs 0.05~0.5 mg/kg 2, 4-D MRLs 0.05 mg/kg, 2, 4-D MRLs 0.5 mg/kg, 2, 4-D MRLs 0.05 mg/kg, 2, 4-D MRLs 0.08 mg/kg, 2, 4-D MRLs 2 mg/kg, 2, 4-D MRLs 0.05 mg/kg, 2, 4-DMRLs 0.2 mg/kg [19] 145 2, 4-D MRLs [17] 2, 4-D 4 3.1%~3.4% Wang [44] 2, 4-D, 2.6 乙烯利 (ethephon),,,,,,, CAC MRLs CAC 41 MRLs, 0.05~20 mg/kg 22 MRLs [15] 54 MRLs, 0.002~30 mg/kg [17] 167 MRLs, 0.05~20 mg/kg [19] 5 表 4 不同国家和组织现行规定常见农产品中 2, 4-D 最大残留限量值 (mg/kg) Table 4 MRLs of 2, 4-D in agricultural products(mg/kg) 1 / / 0.01 / / / / 0.05 / / / / 0.08 / / 0.2 / / 0.08 / / / ( ) / 0.1 / / 0.05 : / 2, 4-D, Liu [43] 2, 4-D, 90.0%~93.4%, RSD 表 5 不同国家和组织现行规定常见农产品中乙烯利的最高残留限量值 (mg/kg) Table 5 MRLs of ethephon in agricultural products(mg/kg) / / / / / / 0.05 / / / 0.5 / / / / 0.5 / / / / / 2 : /

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DOI /j.issn Food Research And Development UPLC-MS/MS 5 0.

DOI /j.issn Food Research And Development UPLC-MS/MS 5 0. 118 217 5 38 1 DOI 1.3969/j.issn.5-6521.217.1.28 Food Research And Development 1 2 1 2 1 2 1 2 1 2 1 2 1. 731 2. 731 - UPLC-MS/MS 5. 1 mol/l ProElut PXC 5 % - Thermo Hypersil Gold mm 2.1 mm 1.9 μm ESI

Microsoft Word 戴 莹-二校 出425

Microsoft Word 戴莹-二校出425