persion met hod. The prefered formulation was determined by factorial design. Ciliary movement in sit utoad palate model and t he changes of pulmonary

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1 2. 3 : (25 2) (60 10) % ( ) : f f GA GB GC GB GA GB GC GB 3. 2 FDA 50 f ( ) : ; ; ; : : ( PNS) : PNS ; PNS : PNS (LUV) ( ) nm ( ) % ; PNS PBS % PNS PNS PBS : PNS :R944 :A : (2004) Notoginseng noside l iposomes : preparation and physiological compatibil ity study SHEN Yang FAN G Xiao2ling 3 ( Depart ment of Pharmaceutics School of Pharmacy Fudan U niversity S hanghai China) KEY WORDS : PNS ; liposomes ; factorial design ; p hysiological compatibility ABSTRACT : AIM : To st udy t he preparation and p hysiological compatibility of pulmonary administ ration wit h Saponius of Panax Notoginseng ( PNS) liposomes. METHODS: PNS liposomes was prepared by t hin2film dis2 : : : (1978 ) E2mail :shen - com. cn : / fax : E2mail address : edu. cn 转载

2 persion met hod. The prefered formulation was determined by factorial design. Ciliary movement in sit utoad palate model and t he changes of pulmonary edema index and pat hology of lungs in rat s were investigated to eval2 uate t he p hysiological compatibility of PNS liposomes. RESUL TS: PNS liposomes were proved to be large unil2 amellar vesicle (L UV) liposomes with spherical shape. The liposomes composed of PC and Ch has a high PNS capture efficiency of % %. The mean vesicle size of liposomes were nm nm which were quite homogeneous in size dist ribution. The lasting time of ciliary movement after t he rinsing of PNS lipo2 somes was the % of the effect of PBS buffer. The pulmonary edema index and pathological changes of lung tissue in PNS liposomes group were significantly different from those in PNS solution group and close to t hose in PBS buffer group. CONCL USION: PNS liposomes has good p hysiological compatibility wit h promising market develop ment and application. ( Panax notogi nseng (Buck. ) F. H. Chen) ) ; ( TEDIA TE2 DIA Company USA) ; ( ( Saponins of ) ; Panax Notoginseng PNS) :SD (250 30) g 2 [3 ] 2. 1 PNS PNS PNS [1 p H ] 250 ml 25 : PNS p H6. 8 ( PBS) mm % 1h PNS RP2HPLC : [2 ] Entrapping Efficiency( %) = C - C 100 % C 2. 2 PNS 1 PNS :ZX298 ( ) ; Zeta ( Nicomp TM 380ZL S Santa Barbara California USA) ; TJ225 PNS (Beckman Coulter TM USA) ; TGL - 16C ( ) ; (MET PNS TL ER AE200) ; LC210A SPD210A ; Hypersil ODS 5 m 150mm 4. 6mm ( ) : (DFC2SQZD201 ( ) ; p H5. 3 PBS p H2. 0 PBS p H7. 8 PBS PNS ) ;

3 PNS 3. 1 PNS 25mg 1 2 ml mg ml mg ml mg ml E) ( F) PNS PNS E F E 75mg ml - 1 F PNS PNS Table 1 [4 ] 2. 3 PNS p H6. 8 PBS Zeta PNS Table 2 ANOVA of the Factorial design results 2. 4 [5 ] PNS : 3. 2 PNS 0. 5mL 30min PNS (L UV) 3mm 3mm ( ) nm ( ) % PNS PNS (75mg ml - 1 ) p H6. 8 PBS % PNS PBS % Table 3 Effect of drugs on ciliary movement 6 A B ( In situ n = 6 gx s) 75mg ml - 1 C 75mg ml d - 1 7d 24h ; d (WW) 60 (WD) ( WW/ WD) 7d 354 ( Factorial design Factorial design and its results E 1 Y( %) S. D. (25mg ml - 1 ) (75mg ml - 1 ) (200mg ml - 1 ) F 1 ( ) F 2 (5 1) Factor E represents the concentration of PNS in PBS buffer ; Factor F presents the ratio of lipid to PNS. Y represents the entrapping Efficiency. - SS v MS F Sig. Tatal F P < E P < EF P < Error Drug p H6. 8 PBS 75mg ml - 1 PNS solution Blank Liposomes PNS liposomes E 2 Lasting time of ciliary E 3 Drug vs p H6. 8 movement (min) PBS( %) mg ml - 1

; 7d PNS PNS 75mg ml - 1 7d 75mg ml - 1 75mg ml - 1 75mg ml - 1 4 PNS PNS Table 4 Pulmonary edema

solution PNS liposomes WW/ WD 3. 54 0. 15 4. 23 0. 16 3. 80 0.

3. 3 ( ) : ( 12 a) PNS : ( ) ( 12b) PNS : ( 12c) PNS a :Physiological saline group b :PNS solution

4 ; 7d PNS PNS 75mg ml - 1 7d 75mg ml mg ml mg ml PNS PNS Table 4 Pulmonary edema index of rats for 7 con2 secutive days drug administration( n = 6 gx s) Group Physiological saline PNS solution PNS liposomes WW/ WD WW represents the weight of wet lung WD represents the weight of dry lung ( ) : ( 12 a) PNS : ( ) ( 12b) PNS : ( 12c) PNS a :Physiological saline group b :PNS solution group PNS c :PNS liposomes group Fig. 1 Pictures of pathological changes of left lung tis2 4 sue in rats 7d PNS PNS PNS PNS PNS PNS PNS 7d PNS PNS PNS PNS p H6. 8 PBS 355

5 : [ 1 ] Xu Q F Fang X L Chen D F. Pharmacokinetics and bioavail2 ability of ginsenoside Rb1 and Rg1 from Panax notoginseng in rats [J ]. Journal of Ethnopharmacology : [ 2 ] Kreuter J. Colloidal Drug Delivery Systems [ M ]. New York : Marcel Dekker Inc [ 3 ]. [ M ]. : [ 4 ]. [ M ]. : [ 5 ]. [J ] (11) : ( ) : ; ; ; : : : Valia2Chien : p H : : :A : (2004) Study on transdermal permeabil ity of ephedra extract in vitro L I Xi2lin XU Lian2ying CAI Zhen2chen ( S hanghai U niversity of TCM S hanghai China) KEY WORDS : Herba ephedrae ; extract ; ephedrine ; transdermal resorption ABSTRACT : AIM : To study the transdermal permeability of ephedra extract. METHODS: With Valia2Chien diffusion cell to the transdermal experiments are used to observe the effect of different factors on the transdermal permeability. RESUL TS: It s t ransdermal resorption constant increased wit h t he increase of t he concent ration of ephedrine and ephedra extract. p H value of the diffusioin medium could influence ephedrine permeability. CON2 CL USION: The result can offer reference in preparation for Ep hera (targeting drug delivery system) ( TDS) ( ) 1. 2 ( 1100 ) ; Valia2 TDS (targeting drug delivery system) ) ; ( ) ; HPLC ( ) 1. 3 ( 1 Chien ( ) ; ( Ephedrine ) ; : : (1963 ). : 356

21 13 D / 2 10 ig ig 10 d 1. 0 g kg /2 2 β- 5 g kg g kg mg kg - 1 ig ig 24 h 5% 1 2% CMC-Na 2. 5 ml 30

21 13 D / 2 10 ig ig 10 d 1. 0 g kg /2 2 β- 5 g kg g kg mg kg - 1 ig ig 24 h 5% 1 2% CMC-Na 2. 5 ml 30 21 13 1 2 1* 2 1. 430065 2. 445000 20 10 5 g kg - 1 5 mg kg - 1 ig 10 d ig HE 1 TFF1 TFF1 P < 0. 01 P < 0. 01 P < 0. 01 P < 0. 05 R285. 5 doi 10. 13422 /j. cnki. syfjx. 2015130100 A 1005-9903 2015 13-0100-05