Microsoft Word - 14——张旭峰_news_.doc

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1 中国北方沿海 3 种养殖扇贝碳氮稳定同位素的组成特征张旭峰 1, 刘瑀 1, 李颖 2, 赵新达 1 2, 王海霞 (1., ; 2., ) 摘要 : 应用元素分析和稳定同位素质谱联用技术 (EA-IRMS) 对中国北方不同养殖地区的虾夷扇贝 (Patinopecten yessoensis) 栉孔扇贝(Chlamys farreri) 和海湾扇贝 (Argopecten irradians) 闭壳肌的碳氮稳定同位素进行了测定, 分析 3 种养殖扇贝的碳氮稳定同位素组成特征结果显示, 青岛和獐子岛虾夷扇贝闭壳肌的 13 C 和 15 N 均差异性显著, 长岛和旅顺栉孔扇贝闭壳肌的 15 N 差异性显著, 莱州牟平和旅顺海湾扇贝闭壳肌的 13 C 和 15 N 均差异性显著闭壳肌的碳氮稳定同位素组成能有效区分不同产地来源的 3 种养殖扇贝关键词 : 虾夷扇贝 ; 栉孔扇贝 ; 海湾扇贝 ; 稳定同位素 ; 产地溯源中图分类号 : S917.4 文献标识码 : A 文章编号 : (2017) DOI: /hykx (Mollusca) (Lamellibranchia) (Pterioida) (Pectinidae),, (Patinopecten yessoensis) (Chlamys farreri) (Argopecten irradians) 3,, 1982,, [1-2], [3], 1982,,, [4],,,, [5] , 97/20/EC, [6], , 97/368/EC,, [7],,,,,,,,,,,,,, DNA,, [8] [9] [10] [11] [12] [13], Ortea Gallardo [14] : ; : : (2015BAD17B05); ( ) [Foundation: National Science & Technology Pillar Program of the Twelfth Five-Year Plan Period, No.2015BAD17B05; National Marine Public Welfare Research Project of China, No ] : (1987-),,,,, zjzhangxufeng@163.com; 瑀 (1965-),,,,,,, ylsibo@foxmail. com Marine Sciences / Vol. 41, No. 2 /

2 Kim [15],, 3 ( ), 3, 3,, (CD) (LS); (LZ) (MP) (LS) ,, 27, 65, 92, 1 1 材料和方法 1.1 样品采集 ,, (QD) (ZZD); 1 3 Fig. 1 Geographical position of scallop sampling points 表 1 不同采样点扇贝样品的体长和体重 Tab. 1 Body length and weight of scallops from different sampling points (cm) (cm) (g) (QD) ± ± ±9.18 (ZZD) ± ± ±7.70 (CD) ± ± ±3.75 (LS) ± ± ±5.20 (LZ) ± ± ±4.75 (MP) ± ± ±6.00 (LS) ± ± ± 样品处理,,, h h,, 80, 1.3 样品测定 0.3~0.5 mg, (Flash EA 1112, Thermo Fisher Scientific) (Delta V Advantage, Thermo Fisher Scientific) 13 C 15 N : X=[(R /R ) 1] 10 3 : X 13 C 15 N; R 13 C/ 12 C 15 N/ 14 N 13 C PDB, 15 N N 2 13 C<0.10, 15 N< 数据分析 3 13 C 15 N, 2 结果 2.1 虾夷扇贝碳氮稳定同位素组成 13 C 15 N 2 13 C ~ , 15 N 5.730~ 6.362; 13 C ~ , 15 N 8.984~9.887, 13 C 15 N 112 / 2017 / 41 / 2

3 , 13 C 15 N 7.992, 13 C, 15 N,,, 13 C, 15 N, 13 C 15 N 2 13 C 15 N Fig C and 15 N values of Patinopecten yessoensis from different sampling points 2.2 栉孔扇贝碳氮稳定同位素组成 13 C 15 N 3 13 C ~ , 15 N 8.621~10.046; 13 C ~ , 15 N 6.856~8.556, 13 C, 15 N, 15 N, 15 N 3 13 C 15 N Fig C and 15 N values of Chlamys farreri from different sampling points 2.3 海湾扇贝碳氮稳定同位素组成 3 13 C 15 N 4 13 C ~ , 15 N 8.920~ 9.670; 13 C ~ , 15 N 8.618~10.142; 13 C ~ , 15 N 6.444~ 4 13 C 15 N Fig C and 15 N values of Argopecten irradians from different sampling points, 种扇贝碳氮稳定同位素组成比较 3 13 C 15 N 5,, ; ( );,,,, 3 讨论, [14-15] Turchini [16], Chaguri [17], (Micropogonias furnieri) 13 C 15 N,,, Marine Sciences / Vol. 41, No. 2 /

4 Fig Stable carbon and nitrogen isotope composition of three types of scallop from different sampling points,, Shimoda [18],, 3 (Decapoda, Thalassinidea, Callianassidae), Suh Shin [19],, Sun [20],,,,,,,, [21],,,,,,,,,,,,,,,, : [1],, 垚垚,. [J]., 2014, 38(6): Shen Yan, Liu Wei, ZhanYaoyao, et al. Establishment of two-dimensional electrophoresis system and analysis of two-dimensional electrophoresis map for gonad and muscle of scallop Patinopecten yessoensis[j]. Marine Sciences, 2014, 38(6): [2],,,. 5 [J]., 2015, 39(8): Pang Yunlong, Ding Jun, Tian Ying, et al. Analysis of shell microstructure and five surface elements of Patinopecten yessoensis at different ages[j]. Marine Sciences, 2015, 39(8): [3],,. (Chlamys farreri) RAPD [J]., 2006, 37(4): Liu Yajun, Kong Xiaoyu, YuZiniu. Investigation of ge- 114 / 2017 / 41 / 2

5 netic diversity in wild populations of scallop Chlamys farreri using RAPD analysis[j]. Chinese Journal of Oceanography and Limnology, 2006, 37(4): [4],,,. [J]., 2009, 33(12): 4-8. LiHongjun, Liu Xiao, DuXuedi, et al. Development of microsatellite markers in bay scallop and their inheritance patterns in an F 1 hybrid family[j]. Marine Sciences, 2009, 33(12): 4-8. [5],,,. [J]., 2010, 25(5): Liu Junrong, Zhang Chaohua, Jiang Haishan, et al. EU food safety management system, special reference to Chinese marine bivalve industry[j]. Journal of Dalian Ocean University, 2010, 25(5): [6] Commission decision of 17 December 1996 establishing the list of third countries fulfilling the equivalence conditions for the production and placing on the market of bivalve molluscs, echinoderms, tunicates, and marine gastropods(97/20/ec)[eb/ol]. http: //eur-lex.europa.eu/ LexUriServ/LexUriServ.do?uri=CELEX: 31997D0020: EN: NOT. [7],,,. [J]., 2013, 32(2): Yu Di, Liu Junrong, Yang Junde, et al. The supply chain traceability management system for Yesso Scallop Patinopecten yessoensis culture[j]. Fisheries Science, 2013, 32(2): [8] Elflein L, Raezke K P. Improved detection of honey adulteration by measuring differences between 13C/12C stable carbon isotope ratios of protein and sugar compounds with a combination of elemental analyzer isotope ratio mass spectrometry and liquid chromatography isotope ratio mass spectrometry (δ13c-ea/lc- IRMS)[J]. Apidologie, 2008, 39(5): [9] Kornexl B E, Roßmann A, Schmidt H L. Improving fruit juice origin assignment by combined carbon and nitrogen isotope ratio determination in pulps[j]. Zeitschriftfür Lebensmittel-Untersuchung und Forschung, 1996, 202(1): [10] Raco B, Dotsika E, Poutoukis D, et al. O H C isotope ratio determination in wine in order to be used as a fingerprint of its regional origin[j]. Food chemistry, 2015, 168: [11] Heaton K, Kelly S D, Hoogewerff J, et al. Verifying the geographical origin of beef: The application of multielement isotope and trace element analysis[j]. Food Chemistry, 2008, 107(1): [12] Molkentin J, Lehmann I, Ostermeyer U, et al. Traceability of organic fish Authenticating the production origin of salmonids by chemical and isotopic analyses[j]. Food Control, 2015, 53: [13] Wu Y, Luo D, Dong H, et al. Geographical origin of cereal grains based on element analyser-stable isotope ratio mass spectrometry (EA-SIRMS)[J]. Food chemistry, 2015, 174: [14] Ortea I, Gallardo J M. Investigation of production method, geographical origin and species authentication in commercially relevant shrimps using stable isotope ratio and/or multi-element analyses combined with chemometrics: An exploratory analysis[j]. Food chemistry, 2015, 170: [15] Kim H, Kumar K S, Shin K H. Applicability of stable C and N isotope analysis in inferring the geographical origin and authentication of commercial fish (Mackerel, Yellow Croaker and Pollock)[J]. Food chemistry, 2015, 172: [16] Turchini G M, Quinn G P, Jones P L, et al. Traceability and discrimination among differently farmed fish: a case study on Australian Murray cod[j]. Journal of agricultural and food chemistry, 2008, 57(1): [17] Chaguri M P, Maulvault A L, Nunes M L, et al. Different tools to trace geographic origin and seasonality of croaker (Micropogonias furnieri)[j]. LWT-Food Science and Technology, 2015, 61(1): [18] Shimoda K, Aramaki Y, Nasuda J, et al. Food sources for three species of Nihonotrypaea (Decapoda: Thalassinidea: Callianassidae) from western Kyushu, Japan, as determined by carbon and nitrogen stable isotope analysis[j]. Journal of Experimental Marine Biology and Ecology, 2007, 342(2): [19] Suh Y J, Shin K H. Size-related and seasonal diet of the manila clam (Ruditapes philippinarum), as determined using dual stable isotopes[j]. Estuarine, Coastal and Shelf Science, 2013, 135: [20] Sun Z, Gao Q, Dong S, et al. Seasonal changes in food uptake by the sea cucumber Apostichopus japonicus in a farm pond: Evidence from C and N stable isotopes[j]. Journal of Ocean University of China, 2013, 12(1): [21],,,. [J]., 2007, 31(9): Xu Qiang, Yang Hongsheng, Wang Hong, et al. Food sources of cultured scallop Chlamys farreri in Sanggou Bay: indicated by fatty acid biomarkers[j]. Marine Sciences, 2007, 31(9): Marine Sciences / Vol. 41, No. 2 /

6 Characteristics of stable carbon and nitrogen isotope compositions of three scallops in the northern coast of China ZHANG Xu-feng 1, LIU Yu 1, LI Ying 2, ZHAO Xing-da 1, WANG Hai-xia 2 (1. College of Environmental Science and Engineering, Dalian Maritime University, Dalian , China; 2. Navigation College, Dalian Maritime University, Dalian , China) Received: May 17, 2016 Key words: Patinopecten yessoensis; Chlamys farreri; Argopecten irradians; stable isotope; origin traceability Abstract: In this research, we study the characteristics of stable carbon and nitrogen isotope compositions of three scallops from different growing areas on the northern coast of China. Isotopic measurements and analysis of the adductor muscles of three scallops (Patinopecten yessoensis, Chlamys farreri, and Argopecten irradians) were performed using an elemental analyzer-isotopic ratio mass spectrometer (EA-IRMS). The results show that 13 C and 15 N in the adductor muscles of P. yessoensis from Qingdao and Zhangzidao were significantly different and can be used to clearly distinguish between scallops from these two sea areas. 15 N in the adductor muscles of C. farreri from Changdao and Lüshun exhibited a significant difference, which can be used to distinguish between the scallops from these two regions. 13 C and 15 N in the adductor muscles of A. irradians from Laizhou, Muping, and Lüshun showed significant differences, which can be used to distinguish between the scallops from these sea areas. In addition, the results indicate that stable carbon and nitrogen isotope compositions of adductor muscles can effectively distinguish scallop origin. ( 本文编辑 : 康亦兼 ) 116 / 2017 / 41 / 2

Microsoft Word - 22 栗志民.doc

Microsoft Word - 22 栗志民.doc 44 6 Vol.44, No.6 2013 11 OCEANOLOGIA ET LIMNOLOGIA SINICA Nov., 2013 (Argopecten irradians concentricus) * 1 1 2 2 (1. 524025; 2. 524022) 2011 10 2012 4 (Argopecten irradians concentricus),,,,,,,, (P