Microsoft Word - 14 胡天宇.doc

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1 44 2 Vol.44, No OCEANOLOGIA ET LIMNOLOGIA SINICA Mar., 2013 PAEs (Pelteobagrus vachelli) * , ( ; ) (GC-FID), (Pelteobagrus vachelli) 5 PAEs, PAEs, PAEs, 5 PAEs,, DEHP, 5 PAEs, h, PAEs, ; ; ; ; X (Phthalic Acid Esters, PAEs, ),, (, 1999;, 2004;, 2007;, 2008) PAEs,,, PAEs (Foster et al, 2000; Makoto et al, 2000; Van Meeuwen et al, 2007; Veronica et al, 2004) (EPA) (DMP) (DEP) (DBP) (DEHP) 6 PAEs (, 1995), DBP 3 PAEs (, 2003) (Darkbarbel Catfish, Pelteobagrus vachelli), 鲿,,,,,,, PAEs, (, 2011;, 2011);, PAEs (, 2007;, 2007) 5 PAEs,, *, , ;, Y ;, 2011C37044, hutianyu51@163.com;,, huhuajun@cjlu.edu.cn :,,, gejian16888@163.com : , :

2 356 44, PAEs,, 1 1.1, (150±20)g 1.0m 0.8m 1.0m,,, (DMP) (DEP) (DBP) (BBP) (DEHP) ( 99% ), ; HPLC, ;, , (FID), HP-5 (0.25μm, 30m 0.25mm),,, 40ml/min;, 1μl : 80 1min, 30 /min 235, 15min, 4 /min 290, 1min, 280, ml 2g,, 5ml,,,, 0.2ml, 1.0μl GC 1.5 DMP DEP DBP BBP DEHP 10mg 100ml,,,, 0.1mg/ml, mg/L,, 1.4, (Y), (X), (r) g/g (QC), 1.4, 5, 1.6 PAEs , 1 5cm 50m 8 10ml, 5 1.4, DMP DEP DBP BBP DEHP PAEs, 1 Fig.1 Sketch map of water distribution in Hangzhou city A: - ; B: - ; C: - ; D: - ; E: - ; F: - ; G: - ; H: -25 ; I: - ; J: - ; K: - ; L: - ; M: - ; N: - 1.7,, 400mg/kg,,, 5 1.4, DMP DEP DBP BBP DEHP PAEs, PAEs -, 2 2.1, μg/g,,

3 2 : PAEs (Pelteobagrus vachelli) 357 ( 1), DMP DEP DBP BBP DEHP 98%, 5% 85%, 15%, ( 2) 表 1 Tab.1 水样和肌肉中 DMP DEP DBP BBP 及 DEHP 曲线方程及相关系数 Regression equation and correlation coefficients of DMP, DEP, DBP, BBP and DEHP in water and muscle DMP DEP DBP BBP DEHP Y = X , Y = X , Y = X , r = Y = X , Y = X , Y = X , Y = X , Y = X , r = Y = X , Y = X , 表 2 肌肉组织中 DMP DEP DBP BBP 及 DEHP 回收率和变异系数 Tab.2 Recovery and precision of DMP, DEP, DBP, BBP and DEHP in muscle tissue (mg/l) (%) (%) DMP ± ± ± DEP ± ± ± DBP ± ± ± BBP ± ± ± DEHP ± ± ± PAEs PAEs ( 2),, DEHP 5 PAEs 2.3 PAEs : t 1/2 = 0.693/K e, ( 3),, t 1/2, K e PAEs h,, PAEs PAEs 3 DMP DEP DBP BBP DEHP5 PAEs (GC-FID), 5 PAEs, PAEs, PAEs HPLC GC GC-MS LC-MS (Lin et al, 2004; Jen et al, 2006; Li et al, 2008; Rios et al, 2010), PAEs,, HPLC,,,,, GC PAEs,, PAEs, PAEs,,,, 5 PAEs, PAEs -,, (Foster et al, 2000; Veronica et al, 2004; Van Meeuwen et al, 2007) PAEs,,,,,

4 Fig.2 2 PAEs The distribution characteristics of PAEs in the water of Hangzhou city 表 3 PAEs 在鱼大脑和肌肉组织中的消除半衰期 (h) Tab.3 The elimination half-time (h) of PAEs in brain and muscle of fish DMP DEP DBP BBP DEHP ,, (, 2008),,,, (Pentikainen et al, 2000), PAEs,,,, PAEs, PAEs PAEs,, PAEs, PAEs 致谢本研究在水样采集处理和实验鱼养殖组织采集匀浆以及样品前处理 GC 检测过程中, 得到了杨丛军冷贞贞李艳刘晓菲李默涵徐琪莹等同学的帮助, 谨致谢忱, :,

5 2 : PAEs (Pelteobagrus vachelli) 359,,, HL-7702., 29(1): 38 40,,, (DEP)., 42(1): ,,, , 23(5): ,,, , 16(6): ,, , 23(7): ,,, , 30(4): 43 47,,, PAEs., 27(6): 79 83,,, , 13(2): 9 14,,, , 27(4): ,,, PAEs (Ctenopharyngodon idellus)., 42(4): Foster P M D, Cattley R C, Mylchreest E, Effects of di-n-butyl phthalate (DBP) on male reproductive development in the rat: implications for human risk assessment. Food and Chemical Toxicology, 38(Suppl.1): s97 s99 Jen J F, Liu T C, Determination of phthalate esters from food-contacted materials by on-line microdialysis and liquid chromatography. Journal of Chromatography A, 1130: Li X, Nie X P, Pan D B et al, Analysis of PAEs in muscle tissue of freshwater fish from fishponds in Pearl River Delta. Journal of Environment and Health, 25(3): Lin X T, Wang X Y, Chen M et al, Analysis of phthalic acid esters of environmental hormone in water using solid phase extraction and high performance liquid chromatography. Research of Environmental Sciences, 17(5): Makoto Ema, Emiko Miyawaki, Kunio Kawashima, Effects of dibutyl phthalate on reproductive function in pregnant and pseudopregnant rats. Reproductive Toxicology, 14(1): Pentikainen V, Erkkila K, Suomalainen et al, Estradiol acts as a germ cell survival factor in the human testis in vitro. J Clin Endocrinol Metab, 85(5): Rios J J, Morales A, Marquez-Ruiz G, Headspace solid-phase microextraction of oil matrices heated at high temperature and phthalate esters determination by gas chromatography multistage mass spectrometry. Talanta, 80(5): Van Meeuwen J A, Burg W T, Piersma A H et al, Mixture effects of estrogenic compounds on proliferation and ps2 expression of MCF-7 human breast cancer cells. Food and Chemical Toxicology, 45(11): Veronica Salazar, Carmen Castillo, Carmen Ariznavarreta et al, Effect of oral intake of dibutyl phthalate on reproductive parameters of Long Evans rats and pre-pubertal development of their offspring. Toxicology, 205(1 2): STUDIES ON DISTRIBUTION CHARACTERISTICS AND ELIMINATION OF PAEs IN THE WATER OF HANGZHOU AND DARKBARBEL CATFISH PELTEOBAGRUS VACHELLI HU Tian-Yu 1, HU Hua-Jun 1, HAN Bao-Yu 2, GE Jian 1, 2, YAO Yuan-Fa 1, WANG Hui 1, WANG Min-Jun 1, QU Ye-Qing 1, YUAN Ping 1 (1. College of Life Sciences, China Jiliang University, Hangzhou, ; 2. Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, Hangzhou, ) Abstract A GC-FID method was established for simultaneous determination of five PAEs chemicals in water and in fish tissues, which was used to study the PAEs distribution characteristics in water of Hangzhou city and elimination rule in darkbarbel catfish, Pelteobagrus vachelli. And the detection results showed that there were significant differences about PAEs chemicals distribution in different districts of Hangzhou and the content of DEHP was the highest. Then five PAEs chemicals were widely distributed in edible tissues of darkbarbel catfish such as muscle and brain. And their elimination half-times lie between 17.33h and 32.54h in muscle and brain. Therefore, all the results showed that five PAEs chemicals were widely distributed in the different areas of Hangzhou District and slowly eliminated from darkbarbel catfish. Key words darkbarbel catfish Pelteobagrus vachelli; PAEs chemicals; distribution characteristics; elimination rule; GC-FID

Microsoft Word - 20120928-275_editing.doc

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