Microsoft Word 张海滨-二校出478

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1 6 2 Vol. 6 No Journal of Food Safety and Quality Feb., 2015 / 张海滨 *, 楼阁, 王娓辰, 张敏, 杨茜 (, ) 摘要 : 目的方法 5 4 kgy 5 kgy 10 kgy, (DEFT ) (APC ), DEFT APC (Dc ), 结果, DEFT, DEFT 0 kgy: 6.9~9.0, 4 kgy: 6.8~8.7, 5 kgy: 6.7~8.9, 10 kgy: 6.9~9.0; APC, APC 0 kgy: 6.0~7.8, 4 kgy: 3.8~5.6, 5 kgy: 3.2~5.5, 10 kgy: 1.2~3.7; 4 Dc, 4 kgy 5 kgy 10 kgy 0 kgy Dc 2.6~4.9, 3.0~5.3, 5.3~ ~2.0 结论 3.0, 5.0 kgy 5.0 kgy 关键词 : ; ; ; Identification of irradiation of herbal medicine by direct epifluorescent filter technique-aerobic plate count method ZHANG Hai-Bin *, LOU Ge, WANG Wei-Chen, ZHANG Min, YANG Xi (Animals, Plants and Foodstuffs Inspection Center, Tianjin Entry-Exit Inspection and Quarantine Bureau, Tianjin, , China) ABSTRACT: Objective To establish a screening method for detecting irradiation of herbal medicine in China. Methods Samples of 5 kinds of herbal medicine, including cardamon, purple perilla, honeysuckle stem, liquorice, and lotus leaf, were irradiated with the doses of 4 kgy, 5 kgy and 10 kgy, one group of unirradiated samples were kept as the 0 kgy. The direct epifluorescent filter technique count unit (DEFT value), aerobic plate count unit (APC value), the difference of DEFT and APC (DC value) were calculated after irradiation. Then the DC value was compared to a special threshold to determine the irradiation situation of the samples. Results The results indicated that with the irradiation dose increased, the difference of DEFT was not significant, the DEFT value was 0 kgy: 6.9~9.0, 4 kgy: 6.8~8.7, 5 kgy: 6.7~8.9, 10 kgy: 6.9~9.0; and the APC value was decreased, the APC value was 0 kgy: 6.0~7.8, 4 kgy: 3.8~5.6, 5 kgy: 3.2~5.5, 10 kgy: 1.2~3.7; the Dc values of the 4 groups were statistically significant, the Dc values of 4 kgy, 5 kgy, 10 kgy and 0 kgy was 2.6~4.9, 3.0~5.3, 5.3~7.3 and 0.4~2.0 respectively. Conclusion When 3.0 was set as the threshold, the samples irradiated with dose of 5 kgy and up could be identified. 基金项目 : ( ) Fund: Supported by Commonweal Research Project of General Administration of Quality Supervision, Inspection and Quarantine of the People s Republic of China ( ) * 通讯作者 :,, Zhanghb@tjciq.gov.cn *Corresponding author: ZHANG Hai-Bin, Senior Engineer, Animals, Plants and Foodstuffs Inspection Center, Tianjin Entry-Exit Inspection and Quarantine Bureau, Tianjin , China. Zhanghb@tjciq.gov.cn

2 2, : / 479 KEY WORDS: irradiation; herbal medicine; direct epifluorescent filter technique; aerobic plate count method 1 引言,, 7300, 87 ( ),,,,, , [1],,,,, [2], [3], ( ),,,,,,,, [4] 70,, 1997 [5], 2004, CAC(Codex Alimentarius Commission) 10 8, [6][7] ( ) [8][9],,, ;, ;, HACCP [10] 2 材料与方法 2.1 样品分析样品 5 : (A) (B) (C) (D) (E) 60 Co 0 kgy 4 kgy 5 kgy 10 kgy, (DEFT) (APC) 分析样品的制备 ( ): 4 kgy 5 kgy 10 kgy,, 0 kgy 60 Co,, 50 g,,, 实验方法方法原理 (DEFT) (APC) APC, DEFT, 450 nm 490 nm APC DEFT,,, 仪器及试验器具,,

3 480 6 ( 0.2 μm, 47 mm), ( 10 μm, 25 mm), ( 0.6 μm, 25 mm),,,, 试剂及培养基 8.5,, (ph 3.0),, 2-,, 检测步骤 g 45 ml, 30 s DEFT APC 80 1% Triton X-100, 0.6 µm 10 µm 2 ml 10 µm 2 ml DEFT, 2.5 ml, 2 min 2.5 ml ph ml 2-,,, (APC) 15 min GB APC 1 ml 15 ml ( 47 ± 2 ) 30 ±1 72 h DEFT DEFT, DEFT,,, 100 oil,, DEFT DEFT, (1) (2) N x M D (1) n F M (2) A V :x DEFT ; N DEFT ; n ; M ; D ( 5 g 45 ml =10 ml/g); F, mm 2, (r 1 = ) ; A, mm 2, ( 0.01 mm) (r 2 = ) ; V, ml; DEFT APC GB , (APC ), (CFU) APC, DEFT APC DEFT APC, (Dc) (3) Dc A B (3) 3 结果 1~ 3 (A) (B) (C) (D) (E) 0 kgy 4 kgy 5 kgy 10 kgy, DEFT 0 kgy:6.9~9.0, 4 kgy:6.8~8.7, 5 kgy:6.7~8.9, 10 kgy:6.9~9.0 APC 0 kgy:6.0~7.8, 4 kgy:3.8~5.6, 5 kgy:3.2~5.5, 10 kgy:1.2~3.7 Dc 0 kgy:0.4~2.0, 4 kgy:2.6~4.9, 5 kgy:3.0~5.3, 10 kgy:5.3~7.3, Dc (P<0.001) 4 讨论 1 2, A~E DEFT, APC (AO) (DNA RNA), DNA, RNA ~ [11] DNA,

4 2, : / A-E DEFT Fig. 1 DEFT values of sample A-E 2 A-E APC Fig. 2 APC values of sample A-E 3 0 kgy 4 kgy 5 kgy 10 kgy A~E Dc Fig. 3 DC values of sample A-E after radiation under 0 kgy, 4 kgy, 5 kgy, 10 kgy RNA, [12] DNA,, AO,, DEFT, DEFT 3, A~E 0 kgy, Dc 0 kgy 0.4~2.0, 4 kgy 2.6~4.9, 5 kgy 3.0~5.3, 10 kgy, 5.3~ , 4 kgy,

5 482 6 ; 3.0, 5 kgy,, 5, 5 结论 / ( ),, 3.0, 5 kgy, Oh [13] DEFT/APC,,,, [11,14,15,16] 参考文献 [1],. [J]., 2011, (1): Song YJ, Xu HF. Foreign trade analysis of herbal in China [J]. Mod Bus Trade Ind, 2011(1): [2]. [J]., 2011, (11): Zhang XD. Trade barriers faced by Chinese herbal export [J]. China Nat Condit Strength, 2011, (11): [3],,,. [J]., 2013, 20(2): Gao J, Du ZY, Li DC, et al. Traditional Chinese medicine situation in America [J]. Pharmaceut Biotechnol, 2013, 20(2): [4]. [J]., 2005, 12(2): 2. Li G. The research on Chinese foreign trade situation and countermeasure in herbal [J]. Chin J Inform TCM, 2005, 12(2): 2. [5],,,. [J]., 2011, 34(3): 482. Zhang LW, Jiang YQ, Lin T, et al. The summarization on herbal irradiation identification research status [J]. J Chin Med Mater, 2011, 34(3): 482. [6],,. [M]. :, 2005: 4 8. Wang XQ, Ha YM, Gao MX. The Irradiation Processing Technique in Food Industry [M]. Beijing: Chemical Industry Publishing House, 2005: 4 8. [7],,,. [J]., 2010, (3): Guo GP, Gao JX, Wang Y, et al. The food irradiation identification method [J]. China Food Nutrit, 2010, (3): [8]. [J]., 2013, 23(5): 1 6. Zheng WJ. Research Progress and Status of Irradiated Food Identification Technology [J]. J Inspect Quarant, 2013, 23(5): 1 6. [9],,,. / (DEFT/APC) [J]., 2010, 24(5): Zhao YF, Li LL, Wang ZD, et al. Detection of irradiated food by using direct epifluorescent filter technique /aerobic plate count method(deft /APC)[J]. J Nuclear Agric Sci, 2010, 24(5): [10] Wirtanen G, Sjoberg AM, Boisen F, et al. Microbiological screening method for indication of irradiation of spices and herbs: A BCR collaborative study [J]. J AOAC Int, 1993, 76(3): [11] Boisen F, Skovgaard N, Ewald S, et al. Quantitation of microorganisms in raw minced meat using the direct epifluorescent filter technique: NMKL collaborative study [J]. Microbiol Meth, 1992, 75(3): [12],,,. DNA RNA [J]., 1993, 28(6): 360. Dong XW, Wang Q, Li C, et al. The observation of DNA and RNA of laryngocarcinoma cell by stain of acridine orange [J]. Chin J Otorhinolaryngol, 1993, 28(6): 360. [13] Oh KN, Lee HJ, Yang S, et al. Screening of gamma-irradiated medicinal herbs by using a microbiological method(deft/apc) [J]. Food Sci Biotechnol, 2002, 11(5): [14] Copln MM, Jehanno D, Bourgeois CM. Detection of irradiated deep1-mfrozen foodstuffs by comparison of DEFT and APC counts [J]. J Appl Bacteriol, 1993, 75: [15] Betts RP, Farr L, Bankes MF. The detection of irradiated foods using the direct epifluorescent filter technique [J]. J Appl Bacteriol, 1988, 64:

6 2, : / 483 [16] Araujo MM, Duarte RC, Silva PV, et al. Villavicencio. 作者简介 Application of the microbiological method DEFT/APC to detect minimally processed vegetables treated with gamma radiation [J]. Radiat Phys Chem, 2009, 78: ( 责任编辑 : 白洪健 ) 张海滨, 高级工程师, 主要研究方向为微生物学农产品品质检验及研究 Zhanghb@tjciq.gov.cn 现代发酵工程在食品工业中的应用专题征稿,,,,,,,,,,,,,, :,,,,,,,, ,, , 投稿方式 : : tougao@chinafoodj.com 食品安全质量检测学报编辑部

Microsoft Word - 4839-4843 20150924002-李建煌-二校全出

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Microsoft Word 张海滨-二校 出478

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