Microsoft Word 盛耀-二校稿完成

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1 5 8 Vol. 5 No Journal of Food Safety and Quality Aug., 2014 / 盛耀 1, 罗云波 1, 谢子鑫 1 1,, 贺晓云 2 1,, 黄昆仑 2, 许文涛 1, 2* (1., ; 2., ) 摘要 : 目的 /, / 方法 /, 20 Linux Apache Mysql PHP(LAMP) /, 结果 /, 300, 200, 108, 结论, /, 关键词 : / ; ; ; ; ; Construction of database of pharmaceutical and industrial transgenic plants SHENG Yao 1, LUO Yun-Bo 1, XIE Zi-Xin 1, HE Xiao-Yun 1, 2, HUANG Kun-Lun 1, 2 1, 2* XU Wen-Tao (1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing , China; 2. The Supervision, Inspection and Testing Center of Genetically Modified Organisms, Ministry of Agriculture, Beijing , China) ABSTRACT: Objective To construct the database of pharmaceutical and industrial transgenic plants, the research group collected published data related with genetically modified (GM) plants as bioreactors. Methods Collect information associated with GM plants. Every GM event contains more than 20 parameters including the expression of exogenous protein, toxicity, allergenicity, clinical data and security level, etc. The database was designed with Linux Apache Mysql PHP (LAMP) and two kinds of retrieval modes, primary retrieval method and advanced retrieval method, were realized for different users. Results In this study, the database of pharmaceutical and industrial transgenic plants was constructed successfully. Presently, we collected more than 300 published data related with GM plants as bioreactors, compiled 200 GM events approximately and uploaded 108 GM events of the information. Conclusion The database is characterized by comprehensiveness, specialty, practicability and innovation, providing information resources for new GM plant varieties development and technological support for government to formulate regulations, approve and supervise pharmaceutical and industrial GM plants. 基金项目 : (2012ZX ) Fund: Supported by Major Project of the Development of New Genetically Modified Organisms (2012ZX ) * 通讯作者 :,,, xuwentao1111@sina.com. *Corresponding author: XU Wen-Tao, Associate Professor, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China. xuwentao1111@sina.com.

2 8, : / 2545 KEY WORDS: pharmaceutical and industrial; transgenic plant; database; exogenous protein; toxicity; allergenicity 1 引言 2013, 1.752, [1], 1200 [2], / /, [3], [4, 5] [6] [7], 37 kg [8] ; Event 3272 α-,, [9], 370, 16 [10] ,, /,, /, [11] / [12, 13],,, 20, [14], [15, 16], ( ) [17, 18], /, / [10], /,,,, (ISAAA) ( (ILSI) (CERA)( se), ( com/dbase.php), ( ( cn/crop_gene.php), (GMDD)( / /,,, / /,, 20, 2 方法学 2.1 数据库信息收集和整理信息收集 /

3 2546 5, G (NCBI) (CNKI) (FDA) (USDA) (EFSA) 300, 信息整理 /,, , 108, 2.2 数据库设计和编程 3 结果 3.1 数据库网站 / ( 19:8000/) ( 3.2 信息检索系统检索方式 ISAAA CERA : ( 1),, /, ( 1); ( 2),, / 架构设计 LAMP, CentOs , Apache, php mysql,, sphinx,, I/O, , 网站设计 CSS+DIV, IE, CI, MVC,,, 2.3 部署实施 Fig. 1 1 Primary retrieval method and level one query results LAMP Apache php mysql,, httpd mysql, ( windows ftp server ), yum sphinx,, 2 Fig. 2 Advanced retrieval method 数据展示, ( 1),,,,

4 第8期盛耀, 等: 药用/工业用转基因植物数据库的构建 2547 括基本信息(图 3-1) 致敏性生物信息学比对结果(图农业医药化工食品环境保护和能源等领域[14] 3-2) 其他相关信息和参考文献目录(图 3-3) 数据库近年来, 药用/工业用转基因植物已经成为转基因领还提供了参考文献下载链接, 供用户进行深入研究域的研究热点目前, 国际上并没有全面的系统的 4 讨评价药用/工业用转基因植物安全性的方法[19] 本研论究构建的药用/工业用转基因植物数据库可以为政府转基因技术自上世纪 80 年代以来发展非常迅速, 机构制定药用/工业用转基因植物安全评价标准提供经过 30 多年的发展, 转基因技术已经被广泛应用到技术支撑, 为科研机构和生物技术公司研发药用/工 Fig. 3-1 Fig. 3-2 图 3-1 二级检索结果基本信息 Level two query results basic information 图 3-2 二级检索结果外源蛋白致敏性生物信息学比对 Level two query results Allergenic bioinformatics comparison of exogenous protein

5 Fig Level two query results other information and reference, /,, / / 30 /, : (1), / ; (2), /, ( ) 20 ; (3),,, ; (4), /, /, : (1), ; (2) /, ; (3), 参考文献 [1] James C. Global status of commercialized biotech/gm crops: 2013 [R]. ISAAA Brief, No. 46, ISAAA: Ithaca NY [2] Brookes G, Barfoot P. The global income and production effects of genetically modified (GM) crops [R]. GM Crops and Food: Biotechnology in Agriculture and the Food Chain, (1): [3] Spök A, Twyman RM, Fischer R, et al. Evolution of a regulatory framework for pharmaceuticals derived from genetically modified plants [J]. Trends Biotechnol, (9): [4] Sala F, Manuela RM, Barbante A, et al. Vaccine antigen production in transgenic plants: strategies, gene constructs and perspectives [J]. Vaccine, 2003, 21(7): [5] Ma JKC, Drake PMW, Chargelegue D, et al. Antibody processing and engineering in plants, and new strategies for vaccine production [J]. Vaccine, 2005, 23(15): [6] Stevens LH, Stoopen GM, Elbers IJW, et al. Effect of climate conditions and plant developmental stage on the stability of antibodies expressed in transgenic tobacco [J]. Plant Physiol, 2000, 124(1): [7] Yang DC, Guo FL, Liu B, et al. Expression and localization of human lysozyme in the endosperm of transgenic rice [J]. Planta, 2003, 216(4): [8],,. [J]., 2011, (1): Pang JF, Huang DG, Wu YM. Research Progress in Corps as Plant Bioreactor [J]. Biotechnol Bull, 2011, (1): [9]. [EB/OL]. ( ).

6 8, : / /02/ html. Food Mate Website. The United States department of agriculture deregulated the genetically modified maize expressing ethanol [EB/OL]. ( ). /2011/02/ html. [10],,,. [J]., 2013, 34(5): Mei XH, Xu WT, He XY, et al. Research Progress in Countermeasures of Food Safety Assessment to New Type of Genetically Modified Plants [J]. J Food Sci, 2013, 34(5): [11],,,. [J]., 2013, 3(5): Liu PL, Li N, Lian Q, et al. Progress in Uing Plant Bioreactor to Produce Pharamatical Protein [J]. Cur Biotechnol, 2013, 3(5): [12] Owen MR, Pen J. Transgenic plants: a production system for industrial and pharmaceutical proteins [M]. John Wiley & Sons, [13] Ma JKC, Chikwamba R, Sparrow P, et al. Plant-derived pharmaceuticals the road forward [J]. Trends Plant Sci, 2005, 10(12): [14],,. [J]., 2013, 21(12): Sheng Y, Xu WT, Luo YB. Commercialization of Genetically Modified Organisms[J]. J Agr Biotechnol, 2013, 21(12): [15] König A, Cockburn A, Crevel RWR, et al. Assessment of the safety of foods derived from genetically modified (GM) crops [J]. Food Chem Toxicol, 2004, 42(7): [16] Domingo JL, Giné BJ. A literature review on the safety assessment of genetically modified plants [J]. Environ Int, 2011, 37(4): [17] EFSA. Safety and nutritional assessment of GM plants and derived food and feed: the role of animal feeding trials [R]. Food and chemical toxicology: an international journal published for the British Industrial Biological Research Association, 2008, 46: S2. [18] Singh OV, Ghai S, Paul D, et al. Genetically modified crops: success, safety assessment, and public concern [J]. Appl Microbiol Biot, 2006, 71(5): [19] Sparrow PAC, Irwin JA, Dale PJ, et al. Pharma-Planta: road testing the developing regulatory guidelines for plant-made pharmaceuticals [J]. Transgenic Res, 2007, 16(2): 作者简介 ( 责任编辑 : 白洪健 ) 盛耀, 硕士研究生, 主要研究方向为转基因食品安全 shengyao314@163.com 许文涛, 副教授, 博士生导师, 主要研究方向为食品安全 xuwentao1111@sina.com

Microsoft Word 聂雪梅.doc

Microsoft Word 聂雪梅.doc 4 4 Vol. 4 No. 4 2013 8 Journal of Food Safety and Quality Aug., 2013 聂雪梅 *, 李立, 孙利, 高飞 (, 100123) 摘要 : 2011 12,, 2007~2011,,,, 关键词 : ; ; ; ; Trade and notification situation on food import and export