β % % 实验动物 SD 48 SPF 189 ~ 217 g SCXK SYXK ~ 25 51% ~ 55% 1. 1

Size: px

Start display at page:

Download "β % % 实验动物 SD 48 SPF 189 ~ 217 g SCXK SYXK ~ 25 51% ~ 55% 1. 1"

段团双
7 years ago
Views:

1 http / /xuebao. jxau. edu. cn Acta Agriculturae Universitatis Jiangxiensis E - mail ndxb7775@ sina. com nm mg /ml SD 5 TC TG HDL - C 800 mg / kg d bw 400 mg / kg d bw 200 mg / kg d bw 45 d TC TG HDL - C H 2 O 2 TC TG HDL - C S A Preparation of Nanometer Propolis and Its Effect on Decreasing Lipoidemia and Anti - oxidation in Rats YAN Wei-yu 1 XIE Guo-xiu 2 DING Huang-jie 1 WU Xiao-bo 1 WANG Zi-long 1 1. Honey Bee Research Institute JAU Nanchang China 2. Institute of Occupational Disease Prevention Nanchang China Abstract Nanometer propolis was prepared with ethyl acetate as the oil phase polysorbate - 80 and Span - 80 as the surfactant. The particle size of nanometer propolis was around 270nm and its concentration was mg /ml. Healthy male SD rats were fed with high - lipid pellet feed for 5 days then blood from eyepit vein was collected to detect the levels of serum TC TG and HDL - C. The rats were randomized into 4 groups. Different feeds for each group were arranged as follows the control group without propolis the high dose group 800 mg /kg BW the middle dose group 400 mg /kg BW and the low dose group 200 mg /kg BW. Then they were fed with nanometer propolis and high - lipid pellet feed in the meantime for 45 d. The blood was collected on the 45th day then the levels of serum TC TG HDL - C and red blood cell RBC hemolyses induced by H 2 O 2 were detected. The result showed that the weight in the high dose group decreased significantly but no difference was observed in TC TG and HDL - C. The hemolysis of red blood cells reduced in the experimental groups but the difference was not significant compaced with that of the control. Key words nanometer propolis decrease lipoidemia anti - oxidation BNB GQN E - mail ywygood @ 163. com

2 β % % 实验动物 SD 48 SPF 189 ~ 217 g SCXK SYXK ~ 25 51% ~ 55% 药物与试剂 % 1% 10% 10% 0. 2% SCXK 主要仪器 Zeta BIC 纳米蜂胶的制备方法 d 1 d 纳米蜂胶粒径的测定粒子表面形态的观察 SEM 溶解度的测定 g 500 ml 1 d r /min 15 min g mg /ml 实验剂量与分组 3 1 TC mg / kg d bw 400 mg / kg d bw 200 mg / kg d bw 实验给药及动物处理 5 d TC TG HDL - C TC 3

3 ml / 100 g bw 45 d 16 h TC TG HDL - C H 2 O 2 诱导氧化溶血试验 r /min 5 min 1 10 min 1% A B 2 ml A 2 ml H 2 O mmol /L B 2 ml min r /min 5 min nm = A B 100% 数据处理 Dunnett t 2 - sided 3 ± t P < mg /ml 270 nm 75 KV 纳米蜂胶对大鼠体重的影响 Fig. 1 The SEM micrograph of nanometer propolis ~ 12 P > P < P > Tab. 1 Effect of nanometer propolis on average weight of rats g Groups 1 Primary weight First week 3 Third week 5 Fifth week 7 Seventh week Final weight ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± * * P < Compare to control group * significant difference P < 纳米蜂胶对大鼠血脂的影响 P < d P > P > 纳米蜂胶 H 2 O 2 诱导氧化溶血的比较 100 mmol /L H 2 O 2

4 % ~ 1. 54% P > Groups Control group Low dose group Middle dose group High dose group Tab. 2 Number of sample 2 Effect of nanometer propolis on lipoidemia of rats TC / mg dl - 1 Primary stage Final stage TG / mg dl - 1 HDL - C / mg dl ± ± ** ± ± ± ± ± ± ± ± ± ± ± ± ± ± ** TC P < ** Extremely significant difference. 3 3 H 2 O 2 Tab. 3 Comparation of hemolysis of red blood cell induced by H 2 O mg /ml /n /% Groups Number of sample RBC hemolysis β ± Control group 6 mg /ml ± Low dose group 270 nm ± Middle dose group AS ± High dose group TC TG 10 H M J J Vassya S Bankova V. Propolis recent advances in chemistry and plant origin J. Apidologie Marcucci MC. Propolis chemical composition biological properties and therapeutic activity J. Apidologie J

5 J Misra C K Das K B Mukherjee S C. Effect of long term administration of dietary β glucan on immunity growth and survival of Labeo ro - hitafiri - geilings J. Aquaculture Gopalakannan A Arul V. Immunomodulatory effects of dietary intake of chitin chitosan and levamisole on the immune system of Cyprinus carpio and control of Aeromonas hydrophilainfection in ponds J. Aquaculture J J J J J J Paulsen S M Engstad R E Robertsen B. Enhanced lysozyme production in Atlantic salmon Salmo salar L. macrophages treated with yeast beta - glucan and bacterial lipopolysaccharide J. Fish & Shellfish Immunol Clerton P Troutaud D Verlhac V. Dietary vitamin E and rainbow trout Oncorhynchus mykiss phagocyte functions effect on gut and on head kidney leucocytes J. Fish & Shellfish Immunol Masahiro S. Current research status of fish immunostimulants J. Aquaculture 櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗 J J J CO 2 J J J J J TiO2 J J K62 L929 MNK28 J J J D J

% 8. 48% 3 80 Alcalase Novozymes Alcalase 2. 4 L Bacillus licheniformis 2. 4 AU /g 1. 2 Hitachi S-4700 JEOL JEM-1200EX Olympus Bu

% 8. 48% 3 80 Alcalase Novozymes Alcalase 2. 4 L Bacillus licheniformis 2. 4 AU /g 1. 2 Hitachi S-4700 JEOL JEM-1200EX Olympus Bu 2011 33 6 1117-1121 http / /xuebao. jxau. edu. cn Acta Agriculturae Universitatis Jiangxiensis E - mail ndxb7775@ sina. com * 311400 Alcalase 2. 4 L TS225. 1 + 6 A 1000-2286 2011 06-1117 - 05 A Study on