PS II PS II GZX-250BS - III 2 d μmol / m 2 s 12 L /12 D FV MV 1 1 cm 2 50 ml

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1 JOURNAL OF NANJING NORMAL UNIVERSITY Natural Science Edition Vol 34 No 3 Sept Porphyra katadai var hemiphylla FV MV 5 ~ μmol / m 2 s 20 d Yield F v /F m 1 2 FV MV 80 μmol / m 2 s 10 μmol / m 2 s 3 MV FV Q945 6 A Photosynthetic Characteristics During the Formation of Reproductive Tissues of Porphyra Katadai var Hemiphylla Wang Yunxia 1 Wang Miaomiao 1 Xia Dongming 2 Zhang Tao 2 Xu Pu 2 Jiang Hongxia 2 1 School of Life Sciences Nanjing Normal University Nanjing China 2 Department of Biology and Food Engineering Changshu Institute of Technology Changshu China Abstract In this study chlorophyll fluorescence parameters Yield F v /F m and oxygen evolution were followed during the formation of reproductive tissues when female vegetative tissues FV and male vegetative tissues MV of Porphyra katadai var hemiphylla were cultured indoor 5 ~ and 80 μmol / m 2 s for 20 days At the same time development of these tissues in culture was observed by microscope The formation of reproductive tissues was closely correlated with the decreasing of photosynthetic activities At the same temperature the tissues cultured under 80 μmol / m 2 s showed a greater extent of maturation than those under 10 μmol / m 2 s did whereas their decreasing degree of photosynthesis was also larger Under the same light intensity the extent of maturation ascended with our increasing temperature At the same time photosynthetic activity of MV culture was the highest at 10 and the lowest at 20 but the decreasing degree of photosynthesis of FV culture was smaller at higer temperature Key words Porphyra katadai var hemiphylla reproductive tissue chlorophyll fluorescence oxygen evolution Porphyra katadai Miura var hemiphylla Tseng et T J Chang Rhodophyta Protoflorideae Bangioideae Bangiales Bangiaceae Porphyra hxjiang10@ 163 com 95

2 PS II PS II GZX-250BS - III 2 d μmol / m 2 s 12 L /12 D FV MV 1 1 cm 2 50 ml μmol / m 2 s 80 μmol / m 2 s LI-185B Li-COR 12 L /12 D 3 3 d 1 2 Water-PAM Walz 4 d Yield F v /F m Yield Yield = ΔF /F' m = F' m - F t /F' m 15 mins F v /F m F v /F m = F m - F o /F m 1 3 YSI 5300A 4 d 1 4 E800 Nikon 5 6 FV MV ± n = 3 ~ 6 t- ANOVA P < FV MV 1a ~ b FV 12 d 1c MV 1d 4 d 1e ~ f 20 d MV 16 d 1 96

3 Fig 1 1 a b c d ~ f Development of vegetative tissues of P katadai var hemiphylla in culture a initial female vegetative tissue b initial male vegetative tissue c differentiation of cells on female vegetative tissue culture d - f differentiation of cells and formation of spermatangia on male vegetative tissue culture d Table 1 The development of vegetative tissues of P katadai 2 FV 10 μmol / m 2 s 80 μmol / m 2 s MV 80 μmol / m 2 s var hemiphylla in culture after 16 days / /μmol / m 2 s FV MV Yield P < μmol / m 2 s 20 d FV % 52% 8% 18% MV Yield d 6% 2a ~ d 5 80 μmol / m 2 s FV MV % + 10 ~ 20% d FV 4 d 20 d 37% 75% MV 10% 16% 2e μmol / m 2 s FV 4 d 4 d ~ 30% ~ 60% ~ 75% ~ 90% 20 d 31% 57% MV Yield 8 d 15% 20 d 7% 2f μmol / m 2 s FV 4 d 31% ~ 49% MV 8 d 7% ~ 10% 2g μmol / m 2 s FV 4 d 4 d 20 d 22% 28% MV 8 d 5% ~ 19% 2h 5 10 Yield MV 8 d ~ 12 d FV 2a b e f μmol / m 2 s MV Yield 8 d FV 31% 14% 20 d 23% 110% μmol / m 2 s MV 12 d FV 36% 20 d 84% μmol / m 2 s MV 16 d FV 33% 20 d 61% μmol / m 2 s FV MV Yield μmol / m 2 s FV MV Yield FV Yield 16 d MV 25% 20 d 53% 2h 97

4 a μmol / m 2 s b μmol / m 2 s c μmol / m 2 s d μmol / m 2 s e μmol / m 2 s f μmol / m 2 s g μmol / m 2 s h μmol / m 2 s 2 Fig 2 Yield FV- MV- Yield of vegetative tissues of P katadai var hemiphylla in culture FV-female vegetative tissue culture MV-male vegetative tissue culture μmol / m 2 s MV F v /F m FV MV F v /F m P < μmol / m 2 s FV MV F v /F m 4 d 8 d 6% ~ 7% 20 d 23% 3a 5 80 μmol / m 2 s FV MV 4 d FV 4 d 20 d 23% 52% MV 17% 34% 3e μmol / m 2 s FV 12 d 4% MV 3b μmol / m 2 s 4 d FV 4 d 20 d 24% 44% MV 13% 30% 3f μmol / m 2 s FV F v /F m 8 d 8% MV 16 d 10% ~ 16% 3c μmol / m 2 s FV MV 4 d 17% 14% 16 d 20 d FV 43% MV 10% 3g a μmol m 2 s b μmol / m 2 s c μmol / m 2 s d μmol / m 2 s e μmol / m 2 s f μmol / m 2 s g μmol / m 2 s h μmol / m 2 s 3 Fig 3 F v /F m FV- MV- F v /F m of vegetative tissues of P katadai var hemiphylla in culture FV-female vegetative tissue culture MV-male vegetative tissue culture 98

5 20 10 μmol / m 2 s FV 4 d 20 d 5% 11% MV 7% 25% 3d μmol / m 2 s FV 4 d 20 d 19% 40% MV 17% 44% 3h 10 μmol / m 2 s FV MV F v /F m 20 FV MV 9% ~ 18% 3a ~ d 80 μmol / m 2 s MV F v /F m 5 8 d FV 13% ~ 38% 10 4 d 13% ~ 25% FV MV 20 FV MV 3e ~ h 4 FV MV μmol / m 2 s μmol / m 2 s μmol / m 2 s 5 10 μmol / m 2 s 16 d FV 16 d 20 d 67% 59% MV 59% 69% 4a 5 80 μmol / m 2 s 4 d FV 4 d 20 d 62% 86% MV 61% 84% 4e μmol / m 2 s 4 d FV 4 d 20 d 64% 89% MV 64% 91% 4f 20 FV MV 10 μmol / m 2 s 12 d 80 μmol / m 2 s 20 d d h a μmol / m 2 s b μmol / m 2 s c μmol / m 2 s d μmol / m 2 s e μmol / m 2 s f μmol / m 2 s g μmol / m 2 s h μmol / m 2 s 4 FV- MV- Fig 4 Oxygen evolution of vegetative tissues of P katadai var hemiphylla in culture FV-female vegetative tissue culture MV-male vegetative tissue culture 10 μmol / m 2 s FV MV d FV MV d MV μmol /g FW /h 4a ~ d 80 μmol / m 2 s FV MV 20 8 d FV MV d e ~ h FV 10 μmol / m 2 s FV Yield F v /F m a c FV 20 d e 80 μmol / m 2 s FV Yield d 15 > 10 > 5 5b FV F v /F m d FV 99

6 f MV 10 μmol / m 2 s MV Yield F v /F m 10 6a c 80 μmol / m 2 s MV Yield F v /F m 20 6b d MV 20 6e f 2 4 FV Yield F v /F m 10 μmol / m 2 s 80 μmol / m 2 s 7 MV Yield F v /F m 10 μmol / m 2 s 80 Fig 7 7 a e i 5 b f j 10 c g k 15 d h l 20 FV μmol / m 2 s Photosynthetic activities of female vegetative tissue FV of P katadai var hemiphylla cultured under different light intensities 100

7 μmol / m 2 s Fig 8 8 a e i 5 b f j 10 c g k 15 d h l 20 MV μmol /m 2 s Photosynthetic activities of male vegetative tissue MV of P katadai var hemiphylla cultured under different light intensities 3 5 ~ μmol / m 2 s MV 4 d 12 d FV PS II 26 PS II 10 ~ 70 μmol / m 2 s 27 5 MV 20 MV FV 5 10 μmol / m 2 s 10 ~ μmol / m 2 s 20 MV a F v /F m 18 a M

8 J Tseng C K Common Seaweeds of China M Beijing Science Press Yan Z M A study on the cultivation of the isolated reproductive cell of Porphyra katadai Miura var hemiphylla Tseng et T J Chang J Collect Ocean Works D D Iwasaki H The life-cycle of Porphyra tenera in vitro J Biological Bulletin Bird C J Chen L C M Mclachlan J The culture of Porphyra linearis Bangiales Rhodophyceae J Canadian Journal of Botany Kapraun D F Luster D G Field and culture studies of Porphyra rosengurtii Coll et Cox Rhodophyta Bangiales from North Carolina J Botanica Marina Gao K Aruga Y Asada K et al Enhanced growth of the red alga Porphyra yezoensis Ueda in high CO 2 concentrations J Journal of Applied Phycology Hawkes M W Sexual reproduction in Porphyra gardneri Smith et Hollenberg Hawkes Bangiales Rhodophyta J Phycologia M Dring M J Makarov V Schoschina E et al Influence of ultraviolet-radiation on chlorophyll fluorescence and growth in different life-history stages of three species of Laminaria phaeophyta J Marine Biology Han T Kong J A Han Y S et al UV-A /blue light-induced reactivation of spore germination in UV-B irradiated Ulva pertusa Chlorophyta J Journal of Phycology J Li Y X Wang G C Xu P et al Induction and characterization of green pigmentation mutant in Porphyra yezoensis Ueda J Aquaculture / D Lin A Wang G Yang F et al Photosynthetic parameters of sexually different parts of Porphyra katadai var hemiphylla Bangiales Rhodophyta during dehydration and re-hydration J Planta Notoya M Kikuchi N Aruga Y et al Porphyra kinositae Yamada et Tanaka Fukuhara Bangiales Rhodophyta in culture J Japanese Journal of Phycology Orfanidis S Culture studies of Porphyra leucosticte Bangiales Rhodophyta from the Gulf of Thessaloniki Greece J Botanica Marina Tang X R Jiang H X Fei X G et al New life cycles of Porphyra katadai var hemiphylla in culture J Journal of Applied Phycology Hannach G Waaland J R Growth and morphology of young gametophytes of Porphyra abbottae Rhodophyta effects of environmental factors in culture J Journal of Phycology J J CO 2 D Jiang C D Shi L Gao H Y et al Development of photosystems 2 and 1 during leaf growth in grapevine seedlings probed by chloropyll a fluorescence transient and 820 nm transmission in vivo J Photosynthetica J M

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