1 : , [ 2, 5, 8], 500 ml, 2,, 4 6 [ 6, 9-1 1] [ 10 ],, 60, ( Sargassum mcclurei) 4 6, 12, 7 ; [ 12] ( S. naozhou-, 5 cm, ense) 4 6, 6 100

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1 South China Fisheries Science Vol. 11, No. 1 Feb., 2015 doi: / j. issn ,, (, ) : ( Sargassum henslouianum) ( ), ,, 3 5,,, 24 h ; 20, 89. 0%, 25 15, 18, 2 3 mm : ; ; ; : S : A : ( 2015) Sexual reproduction of Sargassum henslouianum SUN Liwei, XIE Enyi, QU Yuankai ( College of Fisheries, Guangdong Ocean University, Zhanjiang , China) Abstract: To understand the sexual reproduction and paedomorphosis of Sargassum henslouianum at different temperatures ( 10, 15, 20, 25, 30 ), we researched the reproduction process of S. henslouianum, survival rate and development of zygotes from March 2014 to May Results indicate that 3 5 eggs were released from each female conceptacle. By nuclear division, freshly released eggs that of single nucleus turned into eight-nucleus eggs. After being fertilized the eggs developed into multicellular structures in 24 h without dormancy. The optimum temperatures for ovulation was 20 with survival rate of 89. 0% and for development of seedlings was 25. The first and second buds of branches were observed after being cultured for 15 d and 18 d, respectively, when the average length of seedlings was 2 3 mm. Key words: Sargassum henslouianum; sexual reproduction; temperature; growth and development ( Sargassum henslouianum),,,,,, [ 2-5 ],, 1 m,,, cm, 1 2, [ 6-7 ],,,,,,, [ 1] 3 5 m,,, : ; : : ( 2012B ) ; ( 2012 C0301) : ( ),,, qq. com : ( ),,,, xieenyi@ 163. com

2 1 : , [ 2, 5, 8], 500 ml, 2,, 4 6 [ 6, 9-1 1] [ 10 ],, 60, ( Sargassum mcclurei) 4 6, 12, 7 ; [ 12] ( S. naozhou-, 5 cm, ense) 4 6, 6 100, ( 11 ), 2,,, 30, 30 d, 1. 4, ( % ) = ( ) 100 SPSS ( N E), 4 h,,, 4 5, 4,, 80 L, 20, 6 ( 50 L ), lx [ 12 h ( L) 12 h ( D) ] 30 23, 1 / [ ( ) mm],, 2 ( 1 - b), ( 1 - a), 1. 2, mm), 3 5, ( 1 - c d), OGAWA [ 13 ], ( 1 - b),,, 30 5 cm ( 1 - e), ( GXZ- ( ) cm, ( ) g, 280D, ), ( ) g - 1,, g - 1 SP-350 Olympus ( 1 - f), 2. 2, 24 h 1, ( 2 - b), 1. 3, 6 g 5 1, 1 L, 800 ml, 10 15,, 200, ( X SD), P < ,, [ ( ) mm] ( ), ( ),, ( ( 2 - a), 2 2,

3 南方水产科学 64 图1 亨氏马尾藻生殖托形态及其内部形态 a. 将要排卵的雌性生殖托 ; b. 成熟的雌雄生殖托 ( 右边为雄性 ) ; c. 雌性生殖托的活体切片 ; d. 雌性生殖托的石蜡切片; e. 雄性生殖托的石蜡切片 ; f. 挂满卵的生殖托 Fig. 1 Shape and inside constructure of receptaclees of S. henslouianum a. femal receptacle that will ovulate; b. mature femal and male receptacles ( male receptacle at the right side) ; c. biopsy section of femal receptacle; d. paraffin section of femal receptial; e. paraffin section of male receptial; f. receptacle with eggs adhering to its sruface 图2 受精卵的发育生长 a. 8 核卵; b. 1 核卵 ; c. 第一次横分裂 ; d. 第二次横分裂 ; e. 第一次纵分裂 ; f. 多细胞结构 ; g. 地雷状幼孢子 ; h. 假根长度超过孢子体 ; i j. 孢子体的生长 ; k l. 侧枝的形成 Fig. 2 Development and growth of zygotes a. eight-nuclear eggs; b. one-nuclear egg; c. the first transverse division; d. the second transverse division; e. the first longitudinal division; f. multicellular structure; g. landmine-like seedling; h. length of holdfast which is larger than that of the seedling; i j. growth of seedling; k l. generation of lateral branch 第 11 卷

4 1 : 65 4 h,,, (, 2 ( 2 - c) ; 1 h, 2 ( 2 - d), Tab. 1 Ovulation time and quantity of receptacles at,, different temperatures ( 2 - e), 9 h, 22 h temperature ( 2 - f),, , 48 h ( 2 - g h), , * * 30 : * d ( 2 - i j), 15 ( 2 - k), 18, 2 3 mm( 2 - l),, 30 d, 60 d, cm, ( ), 16. 3%, %, ; %, 5 g ( ) ; %, : d, 60 d h, 6,, ; 15 5 d, 2, 48 h,, ( P < 3 ; d, 0. 05),, , 25 3 d 1) Note: 1 / / d ovulation time * 10 ovulation quantity, ; , ; * * d *. no eggs were observed after 60-day culture under 10 ; * *. receptacles were decayed after 3-day culture under 30 3, 10 ; 15 ; 30, 2 LSD, ( P < 0. 05), 20, 3 a. 30 d ; b. 30 d Fig. 3 Influence of temperature on sexual reproduction a. survival rate of zygote after being cultured for 30 d; b. length of seedlings after being cultured for 30 d

5 66 11, ( S. horneri) , ( P < 0. 05), 15, 20, 2 ( P, >0. 05), 30,, [ 14] 20, [ 10, 12, 15 ], 15 25, ( 2 - a), ( 2 - b), [ 11, 23 ], 2 2, ( 2 - c f),,, ( S. thunbergi ), 20, ( S. vachellianum) [ 10, ] 24 h 25,, 48 h 18 d 2 3 mm ( 2 - k l), : ( 24 h) ( 48 h), 3. 2 [ 18], [ 4],, [ J]. ;,,, [ 12, ], ( P < 0. 05), d,, 30 d 16. 3%, 6010 d, [ 10],,, 60 d lx( 12 L D) 30 2 d, [ 21] ( S. fusiforme) ( 5 ), YOSHIDA [ 22 ] ; ( P < 0. 05), 20, 25 25, [ 1].. [ M]. :, 2000 : [ 2]. [ D]. :, [ 3],,. [ J]., 1998( 3) : , 2000, 21 ( 2) : [ 5],,,. [ J]., 2011, 32( 8 ) : [ 6],,,. [ J]., 2013, 22( 4 ) : [ 7] YU Y Q, ZHANG Q S, TANG Y Z, et al. Establishment of intertidal seaweed beds of Sargassum thunbergii through habitat creation and germling seeding[ J]. Ecol Engin, 2012, 44 : [ 8],,,. [ J]., 2010, 31( 5 ) : [ 9],,,. [ J]., 2013, 40 ( 6) : [ J]., 2013, 34 ( 10 ) : [ 11],,,. [ J]., 2013, 40( 9 ) : [ 12],,,. [ J]., 2011, 35 ( 7 ) :

6 1 : 67 [ 13] OGAWA H. Antheridium development of Sargassum fulvellum and S. kjellmanianum[ J]. Mar Biol, 1976, 38 ( 2) : [ 14],,,. 31( 3) : [ J]., 2010, 31( 5 ) : [ 15] XIE E Y, LIU D C, JIA C, et al. Artificial seed production and cultivation of the edible brown alga Sargassum naozhouense Tseng et Lu[ J]. J Appl Phycol, 2013, 25( 2) : [ 16],,,. [ 22] YOSHIDA G, YOSHIKAWA K, UCHIMURA M, et al. Growth [ J]., 2010, 31( 3) : [ 17 ] YAN X, ZHANG J. Embryology of zygote and development of germling in Sargassum vachellianum Greville ( Fucales, Phaeophyta) [ J]. J Appl Phycol, 2014, 26( 1) : [ 18],,. [ M]. :, 1982: [ 19],. [ J]. :, 2001, [ 20],,,. [ J]., 2011, 35 ( 8 ) : [ 21],. [ J]., 2010, 34 ( 6) : and maduration of Sargassum horneri seeding after long-term storage under a low temperature condition[ J]. Jpn J Phycol, 2001, 66 ( 4) : [ 23],,,. [ J]., 2009, 20 ( 1 ) :

~ a 3 h NCEP ~ 24 3 ~ ~ 8 9 ~ km m ~ 500 m 500 ~ 800 m 800 ~ m a 200

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