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1 Chinese Journal of Applied Entomology 2016, 53(1): DOI: /j.issn 卢少华 * 西藏发现 Q 型烟粉虱 1** 白润娥 1 翟卿 1 王保海 2 闫凤鸣摘要目的 Bemisia tabaci (Gennadius) 方法 3D mtco 结果 mtco Q 结论 Q Q 关键词 Q 1*** New record of the Bemisia tabaci Q biotype in Tibet, China LU Shao-Hua 1** BAI Run-E 1 ZHAI Qing 1 WANG Bao-Hai 2 YAN Feng-Ming 1*** (1. College of Plant Protection, Henan Agricultural University, Zhengzhou , China; 2. Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa , China) Abstract [Objectives] To determine the biotypes of Bemisia tabaci (Gennadius) found in the Tibet Autonomous Region, China. [Methods] Morphological characteristics of whiteflies were observed under a 3D digital microscope, and the biotype of whiteflies collected from Lhasa, Tibet, identified on the basis of variation in the molecular marker mtco. [Results] Analysis of variation in the mtco molecular marker shows that whiteflies collected in Tibet were of the Q biotype. [Conclusion] The results of both morphological identification and molecular analysis show that the whiteflies collected in Lhasa, Tibet Autonomous Region, were of the Bemisia tabaci Q biotype. This is the first report of the Bemisia tabaci Q biotype from the Tibet Autonomous Region. Key words Bemisia tabaci, Q biotype, Tibet, new record, morphological characteristics, molecular detection Bemisia tabaci Gennadius Hemiptera Aleyrodidae De Barro et al Lee et al Boykin et a De Barro et al B - 1 Middle East-Asia Minor 1 Q Mediterranean Dinsdale et al B Chu et al Liu et al Hu et al Q Chu et al Rao et al Q B 2010 Teng et al Pan et al * Supported projects ** First author shaohualu08@163.com *** Corresponding author fmyan@henau.edu.cn Received Accepted

2 214 Chinese Journal of Applied Entomology 53 Jones Li et al mtco Hu et al Q 1 材料与方法 1.1 样品采集地点 E N m Euphorbia pulcherrima 75% 2.0 ml 1.2 形态鉴定 3D KEYENCE VHX-600K 1987 Li 分子检测 B Q mtdna CO PCR TaKaRa mtco PCR 2010 B mtco PCR Shatters et al '-CTAGGGTT- TATTGTTTGAGGTCATCATATATTC-3' 5'-AA- TATCGACGAGGCATTCCCCCT-3' 478 bp Q mtco PCR Shatters et al '-CTTGGTAACTCTTCTGTAGA- TGTGTGTT-3' 5'-CCTTCCCGCAGAAGAAA- TTTTGTTC-3' 303 bp PCR Shatters et al min s 64 1 min 72 1 min min 4 PCR 1.5% 1 TAE Biorad 2 结果与分析 2.1 形态观察成虫 : 1 A 0.85 mm 0.91 mm 1 B 卵 : 1 C 若虫 : D 3 0.6~0.9 mm 2 ( 1 E) 3 ( 1 F) 2.2 分子检测 mtco SCAR(Sequence characterized amplified region) Q B

1期卢少华等: 西藏发现 Q 型烟粉虱 Fig. 1 215 图 1 西藏新记录种 Q 型烟粉虱各期形态 Morphology of Bemisia tabaci Q, a new record to Tibet A. 一品红叶背的不同虫态 B. 成虫 C. 卵 D. 2 龄若虫 E. 3 龄若虫 F. 3 龄若虫皿状孔未能采集到 1 龄若虫和 4 龄若虫 A.

3 1期卢少华等: 西藏发现 Q 型烟粉虱 Fig 图 1 西藏新记录种 Q 型烟粉虱各期形态 Morphology of Bemisia tabaci Q, a new record to Tibet A. 一品红叶背的不同虫态 B. 成虫 C. 卵 D. 2 龄若虫 E. 3 龄若虫 F. 3 龄若虫皿状孔未能采集到 1 龄若虫和 4 龄若虫 A. Adults and immature stages of Bemisia tabaci on the underside of Euphorbia pulcherrima leaf; B. Adult; C. Egg; D. 2nd-instar nymph; E. 3rd-instar nymph; F. Vasiform orifice of a third-instar nymph. (First-instar nymph and fourth-instar nymph were not available in the collection). 虱经过对扩增条带回收测序鉴定 Q 型烟粉虱 COⅠ基因片段图 2 条带 1~4 从本实验室能稳定扩增出 303 bp 条带 B 型烟粉虱能稳定饲养的 Q 型烟粉虱中扩增到 1 条 303 bp 大小的扩增出 478 bp 的条带我们从西藏采集到的烟 mtdna COⅠ基因片段 (图 2 条带 5) 从本实粉虱成虫中均扩增到 1 条 303 bp 大小的 mtdna 验室饲养的 B 型烟粉虱中扩增到 1 条 478 bp 大

4 216 Chinese Journal of Applied Entomology 53 图 2 应用 mtcoⅠ 特异性引物鉴定烟粉虱生物型的 PCR 电泳 Fig. 2 PCR electrophoresis of Bemisia tabaci mtcoⅠ with biotype-specific primers M Marker DL2000 1~4 5 Q 6 B CK M: Marker DL2000; Templates in the PCR reactions are as follows: 1-4: Samples from Lhasa, Tibet; 5: Q biotype from laboratory culture; 6: B biotype from laboratory culture; CK: Control. mtco ( 2 6) mtco Q 3 讨论 Q Q 2012 Q 1999 (Chu et al. 2006) Q Q Q B B Q Q B Q 2012 B Q Q 参考文献 (References) Boykin LM, Shatters RG, Jr Rosell RC, McKenzie CL, Bagnall RA, De Barro PJ, Frohlich DR, Global relationships of Bemisia tabaci (Hemiptera: Aleyrodidae) revealed using Bayesian analysis of mitochondfial CO1 DNA sequences. Molecular Phylogenetics and Evolution, 44(3): Chen CL, Research on ecology of Bemisia tabaci and its prediction, forecasting, early warning. Master thesis. Guiyang:

5 1 : Q 217 Guizhou University. [, :.] Chu D, Pan HP, Guo D, Tao Yi, Liu BM, Zhang YJ, Ecological processes and mechanisms of invasion of the alien whitefly Bemisia tabaci biotype Q in China. Acta Entomologica Sinica, 55(12): [,,,,,, Q., 55(12): ] Chu D, Zhang YJ, Brown JK, Cong B, Xu BY, Wu QJ, Zhu GR, The introduction of the exotic Q biotype of Bemisia tabaci (Gennadius) from the Mediterranean region into China on ornamental crops. Florida Entomologist, 89(2): De Barro PJ, Liu SS, Boykin LM, Dinsdale AB, Bemisia tabaci: a statement of species status. Annual Review of Entomology, 56: Dinsdale A, Cook L, Riginos C, Buckley YM, De Barrao PJ, Refined global analysis of Bemisia tabaci (Hemiptera: Sternorrhyncha: Aleyrodoidea: Aleyrodidae) mitochondrial cytochrome oxidase 1 to identify species level genetic boundaries. Annals of the Entomological Society of America, 103(2): Hu J, De Barro P, Zhao H, Wang J, Nardi F, Liu SS, An extensive field survey combined with a phylogenetic analysis reveals rapid and widespread invasion of two alien whiteflies in China. PLoS ONE, 6(1): e Ji K, Bai RE, Tang QB, Jiang JW, Zhang DS, Yan FM, Biotype identifications and phylogenic analysis of overwintering Bemisia tabaci in protecting fields of Henan Province. Journal of Henan Agricultural University, 44(5): [,,,,,, , 44(5): ] Jones DR, Plant viruses transmitted by whiteflies. European Journal of Plant Pathology, 109(3): Ko CC, Chen CN, Wang CH, A review of taxonomic studies on the Bemisia tabaci species complex. Taiwan Entomology, 22: [,,, (Bemisia tabaci species complex)., 22: ] Lee W, Park J, Lee GS, Lee S, Akimoto S, Taxonomic status of the Bemisia tabaci complex (Hemiptera: Aleyrodidae) and reassessment of the number of its constituent species. PLoS ONE, 8(5): e Li JJ, Tang QB, Bai RE, Li XM, Jinag JW, Zhai Q, Yan FM, Comparative morphology and morphometry of six biotypes of Bemisia tabaci (Hemiptera: Aleyrodidae) from China. Journal of Integrative Agriculture, 12(5): Liu SS, De Barro PJ, Xu J, Luan JB, Zang LS, Ruan YM, Wan FH, Asymmetric mating interactions drive widespread invasion and displacement in a whitefly. Science, 318(5857): Lu SH, Li JJ, Liu MY, Bai RE, Tang QB, Yan FM, Comparative analysis of the competitiveness between B and Q biotypes of Bemisia tabaci under laboratory conditions. Scientia Agricultura Sinica, 48(7): [,,,,,, B Q., 48(7): ] Luo C, Zhang ZL, An overview on researches of Bemisia tabaci (Gennadius). Beijing Agricultural Science, 18(Suppl.): 5 6. [, Bemisia tabaci (Gennadius)., 18 (Suppl.): 5 6.] Pan HP, Studies on the ecological mechanism of displacement of Bemisia tabaci B biotype by Q biotype. Doctoral dissertation. Beijing: Chinese Academy of Agricultural Sciences. [, Q B.. :.] Pan HP, Chu D, Ge DQ, Wang SL, Wu QJ, Xie W, Jiao XG, Liu BM, Yang X, Yang NN, Su Q, Xu BY, Zhang YJ, Further spread of and domination by Bemisia tabaci (Hemiptera: Aleyrodidae) biotype Q on field crops in China. Journal of Economic Entomology, 104(3): Pan HP, Ge DQ, Wang SL, Wu QJ, Xu BY, Xie W, Zhang YJ, Replacement of B biotype Bemisia tabaci by Q biotype B. tabaci in some areas of Beijing and Hebei. Plant Protection, 36(6): [,,,,,,, Q B., 36(6): ] Rao Q, Luo C, Zhang H, Guo X, Devine GJ, Distribution and dynamics of Bemisia tabaci invasive biotypes in central China. Bulletin of Entomological Research, 101(1): Ren SX, Qiu BL, Whitefly of China and the Sustainable Control. Guangzhou: Guangdong Science and Technology Press [,, : ] Shatters Jr. RG, Powell CA, Boykin LM, He LS, McKenzie CL, Improved DNA barcoding method for Bemisia tabaci and related Aleyrodidae: development of universal and Bemisia tabaci biotype-specific mitochondrial cytochrome c oxidase I polymerase chain reaction primers. Journal of Economic Entomology, 102(2): Teng X, Wan FH, Chu D, Bemisia tabaci biotype Q dominates other biotypes across China. Florida Entomologist, 93(3): Yan FM, Taxonomic study on Aleyrodinae from China. Master dissertation. Shanxi: Northwest China Agricultural University. [ Aleyrodinae.. :.]

542 Journal of Environmental Entomology 38 vaporariorum Westwood 2000 2007 Aleyrodes proletella Linnaeus 2014 1998 1992 1995 1 Pealius mori Takahashi

542 Journal of Environmental Entomology 38 vaporariorum Westwood 2000 2007 Aleyrodes proletella Linnaeus 2014 1998 1992 1995 1 Pealius mori Takahashi Journal of Environmental Entomology May 2016 38 3 541-549 ISSN 1674-0858 doi 10. 3969 /j. issn. 1674-0858. 2016. 03. 14 1 2 3* 4 1* 1. 225009 2. 311300 3. 830052 4. 830006 5 6 Aleyrodes proletella Linnaeus