826 微生物学通报 2008, Vol.35, No La Forlge - (allethrin), ( ),, 1973,,, 70 [1] ,, ; , , [2],,, [3,4]

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1 微生物学通报 MAY 20, 2008, 35(5): 825~829 Microbiology 2008 by Institute of Microbiology, CAS 专论与综述拟除虫菊酯类农药降解菌及降解酶的研究概况王兆守 1,3,4* 刘丽花 1,2 陈小兰 2 邵宗泽 4 ( ) ( ) ( ) ( ) 摘要 : 拟除虫菊酯类农药目前已成为我国出口的蔬菜水果中主要的 3 类农药残留之一, 引起急慢性中毒事件也越来越多, 对人类水生生物和自然环境造成很大危险而农药生物降解作为去除农药污染的有效手段, 逐渐成为环境科学研究的热点重点综述了拟除虫菊酯类农药降解菌的分离降解酶的提取纯化降解机理固定化研究, 并对以后要解决的问题进行了展望关键词 : 拟除虫菊酯, 降解菌, 降解酶, 纳米材料, 固定化 The Research Survey of the Degrading-bacteria and Degrading-enzyme of Synthetic Pyrethroid Insecticides WANG Zhao-Shou 1,3,4* LIU Li-Hua 1,2 CHEN Xiao-Lan 2 SHAO Zong-Ze 4 (1. Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen ) (2. Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen ) (3. State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen ) (4. Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography State Oceanic Administration, Xiamen ) Abstract: Synthetic pyrethroid insecticides have become one of the three major kinds of pesticide residues in the exported vegetabes and fruits in China nowadays. They also caused more and more acute or chronic poisoning affair and do great damage to human beings, aquatic biology and natural environment. As an efficient way to remove pesticides pollution, the biodegradation of pesticides has gradually become the hot issue in the field of environmental sciences. The study on the separation of degrading-bacteria and the extraction, purification, mechanism and immobilization of the degrading-enzyme of pyrethroids is reviewed in this paper. And the problems under resolved in the future work are also prospected. Keywords: Synthetic pyrethroid insecticides, Degrading-bacteria, Degrading-enzyme, Nanomaterials, Immobilization 基金项目 : (No. 2007F3094); (No X071C3); (No. MEL0603); (No ); (No. HY200601) * 通讯作者 :Tel: ; : wzs@xmu.edu.cn 收稿日期 : ; 接受日期 :

2 826 微生物学通报 2008, Vol.35, No La Forlge - (allethrin), ( ),, 1973,,, 70 [1] ,, ; , , [2],,, [3,4], [5,6] ;, [7] [8],,,,, [9], [10] L3, Aspergillus nomius, 28 ph h 29.2, 120 h 95.3, ; [11] (HCH) BHC-A, (Sphingomonas sp.), 15 d BHC-A α- β- γ- δ-hch ,,, 1 菌株的分离筛选及降解特性研究 [12] ph7~8 30 qw5, ; [13] GC-MS (Rhodococcus sp.)cdt3, 30 ph ;, [14] c1f6, Grant [15,16], r/min 14 d, 250 mg/l 农药降解酶的提取及粗酶研究,,, [17] (Alcaligenes sp. YF11) ph5.0~ phl0.5 [18], TR2 35 ph8.0, 20 min 84.6 Sogorb [19],,, 3 农药降解酶的纯化 Malony [20] (Bacillus cereus SM3) (permethrinase) 37 ph7.0

3 : 827,, [21] CP-1, mol/l Na 2 HPO 4 -NaH 2 PO 4, DEAE-52 CM-52,, 15.0 U/mg 55.4 U/mg 85.0 U/mg; ,, CP-1 60 h pent-2-ene-carboxylic acid) 19 E [Cyclopropanecarboxylic acid 2,2-dimethyl-3-(2-methyl-1-propenyl)] 0.12 G[2-Propa- nal (1,1-dimethyl ethyl)-methyl] ; 72 h, G[2-Propanal (1,1-dimethyl ethyl)-methyl], G,,, 4 降解机理,,,,, [22] [23] [18] 1 图 1 酯酶水解拟除虫菊酯类农药的通式 Fig. 1 Fomula for the course of esterase hydrolyzing pyrethroids [24] GC-MS, Alcaligenes sp. YF11 12 h C D (C D 2), 24 h C D, C D, Alcaligenes sp. YF11 Paingankar [25],, GC-MS,,, 48 h 20 D(2-Ethyl-1,3-dimethyl cyclo- 图 2 化合物 C 和化合物 D 的分子结构式 Fig. 2 Molecular structure of compounds of C and D 5 农药降解酶的固定化, [26],,, 10 7 mol/l, ; [27] - (p-co-tapc) (GCE), ( ), mol/l mol/l mol/l; [28],, g/l g/l, 5.1 降解酶的常规固定化技术 [29,30] Alcaligenes sp. YF11,

4 828 微生物学通报 2008, Vol.35, No.5,,,, 35 ph8 90 ~100,,, [31],, 3 Lineweaver- Burk K m= nmol/ml, ph6.8 35, 80,,, 5.2 降解酶在纳米材料中的固定化, [32], 2 nm, 80,,,,,, 1894 Fenton(Fe 2+ /H 2 O 2 ), [33], Li [34,35] NaY/ H 2 O 2,, Ha [36], - 58, Suman [37] -,,, 6 小结与展望,, ;,,,,,,, [38], 参考文献 [1],,,.., 2002, 23(1): [2],.. :, 2007, pp [3] Al-Makkawy HK, Magdy DM. Persistence and accumulation of some organic insecticides in Nile water and fish. Resources Conservation and Recycling, 1999, 27(1-2): [4] Kolaczinski JH, Curtis CF. Chronic illness as a result of low-level exposure to synthetic pyrethroid insecticides: a review of the debate. Food and Chemical Toxicology, 2004, 42(5): [5] Nina G, Friedhelm D. Influence of pyrethroids and piperonyl butoxide on the Ca 2+ -ATPase activity of rat brain synaptosomes and leukocyte membrane. International Immunopharmacology, 2005, 5(2): [6],,.., 2005, 50(22): [7] Anders C, Valery EF. Consequences of a short pulse of pesticide exposure for survival and reproduction of Gammarus pulex. Aquatic Toxicology, 2004, 67(3): [8],.., 2003, 22(1): 1 5.

5 : 829 [9],,.. :, 1997, pp [10],,,. L3., 2006, 25(5): [11],,,. (HCH) Sphingomonas sp. BHC-A., 2005, 45(5): [12],,,.., 2003, 40(1): [13],,,. CDT3., 2005, 25(4): [14],,,.., 2005, 25(7): [15] Grant RJ, Daniell TJ, Betts WB. Isolation and identification of synthetic pyrethroid-degrading bacteria. Journal of Applied Microbiology, 2002, 92(3): [16] Grant RJ, Betts WB. Biodegradation of the synthetic pyrethroid cypermethrin in used sheep dip. Letters in Applied Microbiology, 2003, 36(3): [17],,,. Alcaligenes sp.yf11., 1998, 20(6): 5 7. [18],,,. TR2. ( ), 2007, 24(3): [19] Sogorb MA, Vilanova E. Enzymes involved in the detoxification of organophosphorus, carbamate and pyrethroid insecticides through hydrolysis. Toxicology Letters, 2002, 128(1-3): [20] Maloney SE, Maule A, Smith ARW. Purification and preliminary characterization of permethrinase from a pyrethroid- transforming strain of Bacillus cereus. Appl Environ Microbiol, 1993, 59(7): [21],.., 2005, (12): [22],.., 2007, 19(1): [23],,,. (Carassius auratus)., 2006, 6(3): [24],,,. Alcaligenes sp. YF1., 1998, 20(6): 5 7. [25] Mandar P, Manish J, Dileep D. Biodegradation of allethrin, a pyrethroid insecticide, by an Acidomonas sp.. Biotechnology Letters, 2005, 27: [26],,.., 2003, 24(5): [27],,,. -., 2005, 24(6): [28],,.., 2007, 26(9): [29],,.., 1999, 5(Suppl): [30],,.., 1999, 1(1): [31],,.., 2006, 34(17): [32].. :, 2000, pp [33] Tao X, Ma WH, Zhang TY, et al. Efficient photooxidative degradation of organic compounds in the presence of iron tetrasulfophthalocyanine under visible light irradiation. Angewandte Chemie International Edition, 2001, 40: [34] Li J, Ma WH, Huang YP, et al. Oxidative degradation of organic pollutants utilizing molecular oxygen and visibe light over a supported catalyst of Fe(bpy) 2+ 3 in water. Applied Catalysis B: Environmental, 2004, 48: [35] Li J, Ma WH, Huang YP, et al. A highly selective photooxidation approach using O 2 in water catalyzed by iron(ii) bipyridine complex supported on NaY zeolite. Chemical Communication, 2003, [36] Ha TH, Jeong JY, Chung BH. Immobilization of hexa-arginine tagged esterase onto carboxylated gold nanoparticles. Chemical Communication, 2005, [37] Suman S, Rahul S, Malhotra BD. Immobilization of cholesterol esterase and cholesterol oxidase onto sol-gel films for application to cholesterol biosensor. Analytica Chimica Acta, 2007, 582(2): [38],.., 2005, 19(4):

Microsoft Word - 793-797 tb20150504赵宏宇s-高校教改纵横.doc

Microsoft Word - 793-797 tb20150504赵宏宇s-高校教改纵横.doc 微生物学通报 Microbiology China tongbao@im.ac.cn Apr. 20, 2016, 43(4): 793 797 http://journals.im.ac.cn/wswxtbcn DOI: 10.13344/j.microbiol.china.150504 高校教改纵横生物工程专业发酵课程群建设探索 * 赵宏宇