1 65 or boiled in water for abdominal pain and diarrhea in the folk. Homonoia ripariausually found nearby stream and river was used as seasoning to ma

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1 Guihaia Jan http/ /journal.gxzw.gxib.cn http/ / DOI /guihaia.gxzw J WANG MZUO GYTANG JFet al. Folk utilization and antimicrobial activity of three medicinal plants in Xishuangbanna J. Guihaia * Psidium guajava Phyllanthus emblica Homonoia riparia 3 80% 3 MIC 98~ 390 μg ml -1 MBC 98~ 781 μg ml -1 MIC MBC 6 250~ μg ml -1 3 Q946 A Folk utilization and antimicrobial activity of three medicinal plants in Xishuangbanna WANG Meng 1 ZUO Guo-Ying 2 TANG Jin-Feng 2 XU You-Kai 1 TANG Jian-Wei 1 LI Ren 1 HU Hua-Bin 1* 1. Xishuangbanna Tropical Botanical GardenCASMengla666303YunnanChina2. Kunming General Hospital of Chengdu Military CommandKunming China AbstractXishuangbanna is the birth-place of ethnobotanical study in China and it has high level of biological and cultural diversity. The local people of Xishuangbanna have collected and inherited a great amount of indigenous knowledge and experience when they struggled to survive from the rugged environment and tropical diseases. Our ethnobotanical and pharmacological survey showed that Psidium guajavaphyllanthus emblicaand Homonoia riparia were commonly utilized as daily seasonings fruits vegetables and medicines by local villagers in Xishuangbanna. The antimicrobial activity test of these three plants was conducted to verify their usage. Our field survey showed that Psidium guajava and Phyllanthus emblica were frequently found growing in villagers yardsand they were both used as vegetables and fruits by the DaiHani and Jinuo people in Xishuangbanna. On the other hand the young leaves of Psidium guajava were eaten raw FY Supported by the Special Grant for Basic Work of Science and Technology from the Ministry of Science and Technology 2012FY wangmeng@ xtbg.ac.cn * huhb@ xtbg.ac.cn

2 1 65 or boiled in water for abdominal pain and diarrhea in the folk. Homonoia ripariausually found nearby stream and river was used as seasoning to make dai flavor sour fish by Dai people. According to literature the leaves of the three medicinal plants are boiled in water and externally used for skin disease such as pruritus. For the antimicrobial activitythe minimal inhibitory concentration and minimal bactericidal concentration MIC and MBCwere determined. The 80% ethanol leaves extracts from them showed significant activities on Stphylococcus aureus and Escherichia coliwith the MIC and MBC ranging from μg ml -1 and μg ml -1 respectively. In additionthe leaves extracts from Psidium guajava and Homonoia riparia showed weak activities against Pseudomon asaeruginosa with the MIC and MBC ranging from μg ml -1. The relatively obvious antimicrobial activities of the selected three medicinal plants used by local villagers for stomach and skin diseases showed that these plants had the rationality and potential of medicinal applications. Key wordsxishuangbannaethnobotanymedicinal plantantimicrobial activitypsidium guajavaphyllanthus emblicahomonoia riparia ~ Liu et al g 80% Psidium guajava Phyllanthus emblica ATCC25923 Staphylococcus aureus ATCC25922 Homonoia riparia3 Escherichia coli ATCC27853 Pseudomon asaeruginosa ATCCY0109 Mo- 3 nilia albican MIC MBC 98 ~ μg ml -1 1 MIC 2016 MIC MIC 3~5 1.1 MH h MBC

3 Table 1 Three medicinal plants used in Xishuangbanna Botanical name Vernacular name Parts used and use Documentary record Leaf Psidium guajava Linn. Dma li ganguo su dan Hma li gandie ma Jma li ganma gui / / Fruits are eaten raw as fruityoung leaves are eaten raw or boiled in water for diarrhea Young leaves are used as decoction for diarrhealeaves are boiled in water for skin disease Phyllanthus emblica Linn. Dgan lan guoma han beng Hgan lan guoxi qia ha Jgan lan guosen chao Fruits are eaten raw as fruit or cooked as vegetable Whole plant is treated for amygdalitisleaves are boiled in water for or drank as wine Barks are eaten raw as seasoningroot are boiled in water for hypertensionleaves are cooked for reducing skin disease phlegm Homonoia riparia Lour. Dguo hai yi Hpa huo Jmei beng Leaves are used as seasoning or as medicine Barks are eaten raw as seasoning Whole plant is treated for pneumonia or urinary system infectionleaves are pounded or boiled in water for skin disease D. H. J. NoteD. DaiH. HaniJ. Jinuo. Table 2 2 μg ml -1 Antimicrobial activity of the three medicinal plants Unitμg ml -1 Microbial strain Psidium guajava Phyllanthus emblica Homonoia riparia Positive control MIC /MBC MIC /MBC MIC /MBC MIC /MBC SA 195 / / /781 1 /2 EC 98 /98 98 / /195 2 /2 PA / / / /2 MA / / / 16 /32 SA. EC. PA. MA. - >12500 μg ml -1 NoteSA. Staphylococcus aureusecescherichia colipa. Pseudomon asaeruginosama. Monilia albicanpositive control. vancomycin Staphylococcus aureus amikacin Escherichia coli and Pseudomon asaeruginosa and fluconazole Monilia albican - the value > μg ml -1. ma li gan gan lan guo 39% 76% ma li gan 29% gan lan guo 26% %

4 MIC 98 ~ 390 μg ml -1 MBC 98 ~ 781 μg ml -1 MIC MBC ~ μg ml -1 ABDOLBASET G GERHARD L LIU F et al Ethnobotanical 3 study of medicinal plants utilised by Hani ethnicity in naban river watershed national nature reserve Yunnan China J. J Ethnopharmocol CHEN GJIANG DXWANG YX2009. Echerichia coli 3 distribution and resistance analysis J. Chin J Nosocomiol J EDIED BY YUNNAN INSTITUTE OF MATERIA MEDICA The annals of national medicine in Yunnan Vol M. Kunmingthe Nationalities Publishing House of Yunnan M EDIED BY YUNNAN INSTITUTE OF MATERIA MEDICA 2006Abdolbaset et al The annals of national medicine in Yunnan Vol. 2 M. Kunmingthe Nationalities Publishing House of Yunnan M EDIED BY YUNNAN INSTITUTE OF MATERIA MEDICA The annals of national medicine in Yunnan Vol M. Kunmingthe Nationalities Publishing House of Yunnan Abdolbaset et al 2011 M FANG LY Research on traditional use of aquatic plants of 2014 Xishuangbanna Dai people D. XishuangbannaXishuangbanna Tropical Botanical GardenChinese Academy of Sciences D GU W YANG J YANG FM et al A preliminary study of traditionalwild medicinaledible plants in Xishuangbanna 2006 Yunnan China J. Plant Divers J HUAI HY HA SBG WANG YH et al Discussion on misunderstandings of ethnobotany J. Chin Bull Bot J LI QJ The status of non-timber forest products used by Dai people in Xishuangban D. XishuangbannaXishuangbanna Tropical Botanical GardenChinese Academy of Sciences D. 53. LIN YF YI Z ZHAO YH Chinese Dai medicine colorful illustrations M. KunmingThe Nationalities Publishing House of

5 68 37 Yunnan Res M. 148 J WANG F WANG L DONG L et al Study on antibacterial activity LIU BGUO ZYBUSSMANNet al2016. Ethnobotanical and identification of extract from guava leaf J. J Mol Sci approaches of traditional medicine studies in southwest China J. J Ethnopharmocol LIU YZ LI HX XU LJ et al Modern research overview and application prospect analysis on Phyllanthus emblica both as diet and medicine J. Chin Trad Herb Drug J WANG JR LONG CL Ethenobotanical study of traditional edible plants of Jinuo nationality J. Acta Bot Yunnan J J LONG CL A BZ WANG Het al Biodiversity management and utilization in the context of traditional culture of Jinuo society in S Yunnan China J. Acta Bot Yunnan YU PH XU ZF HUANG YL The study on traditional cultivated plants in Dai villages of Xishuangbanna J. Acta Bot Yunnan J J YUAN WC2013. Adaptive resistance and drug - resistance PAN YM Traditional beverage plants used by Dai villagers in Xishuangbanna Yunna China D. XishuangbannaXishuangbanna Tropical Botanical Garden Chinese Academy of Sciences regulation in methicillin-resistant Staphylococcus aureus D. ChongqingThird Military Medical University MRSA D. 8. D. 50. INSTITUTUM BOTANICUM KUNMINGENSE ACADEMIAE SINI- PEI SJ Brief discussion on ethnomedicine research and new CAE EDITA Flora Yunnanica Vol. 7 M. Beijing drug development of China J. J Yunnan Univ Trad Chin Med Science Press M J INSTITUTUM BOTANICUM KUNMINGENSE ACADEMIAE SINI- SHAO M FAN CLWANG Y et al Advances on chemical CAE EDITA Flora Yunnanica Vol. 10 M. Beijing constituents and pharmacological effects of psidium guajava J. Nat Prod Res Dev Science Press M J. ZHONG ZGZENG CL Antimicrobial activity in vitro of TANG JF LI L ZUO GY Screen of antimicrobial activity in vitro of 51 Chinese herbal medicines J. Lishizhen Med Mat Med extract from Phyllanthus emblica leaf J. Chin Trad Herb Drug J 櫕櫕櫕櫕櫕櫕櫕櫕櫕櫕櫕櫕櫕櫕櫕櫕櫕櫕櫕櫕櫕櫕櫕櫕櫕櫕櫕櫕櫕櫕櫕櫕櫕櫕櫕櫕櫕櫕櫕櫕櫕櫕櫕櫕櫕櫕 79 Continue from page 79 evolutionary origins of plant-specific transcription factor DNAbinding domains J. Plant Physiol Biochem YAN HJIA HCHEN Xet al2014. The cotton WRKY transcription factor GhWRKY17 functions in drought and salt stress in transgenic Nicotiana benthamiana through ABA signaling and the modulation of reactive oxygen species production J. Plant Cell Physiol YANG PCHEN CWANG Zet al1999. A pathogen- and salicylic acid-induced WRKY DNA-binding activity recognizes the elicitor response element of the tobacco class I chitinase gene promoter J. Plant J YE SJIANG YDUAN Yet al2014. Constitutive expression of the poplar WRKY transcription factor PtoWRKY60 enhances resistance to Dothiorella gregaria Sacc. in transgenic plants J. Tree Physiol YU DCHEN CCHEN Z2001. Evidence for an important role of WRKY DNA binding proteins in the regulation of NPR1 gene expression J. The Plant Cell YU FHUAXIA YLU Wet al2012. GhWRKY15a member of the WRKY transcription factor family identified from cotton Gossypium hirsutum L. is involved in disease resistance and plant development J. BMC Plant Biol ZHANG YWANG L2005. The WRKY transcription factor superfamilyits origin in eukaryotes and expansion in plants J. BMC Evol Biol51. ZHAO JWWANG JLAN LLet al2007. Analysis of gene expression profiles in response to Sclerotinia sclerotiorum in Brassica napus J. Planta ZHENG LLIU GMENG Xet al2013. A WRKY gene from Tamarix hispidathwrky4mediates abiotic stress responses by modulating reactive oxygen species and expression of stress-responsive genes J. Plant Mol Biol ZHOU LWANG NNGONG SYet al2015. Overexpression of a cotton Gossypium HirsutumWrky geneghwrky34in Arabidopsis enhances salt-tolerance of the transgenic plants J. Plant Physiol Biochem ZHOU QYTIAN AGZOU HFet al2008. Soybean WRKY-type transcription factor genesgmwrky13gmwrky21 and Gm- WRKY54confer differential tolerance to abiotic stresses in transgenic Arabidopsis plants J. Plant Biotechnol J

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中国农学通报 2013,29(04):39-43 Chinese Agricultural Science Bulletin 中药荆芥最新研究进展 1,2 1,2 赵立子, 魏建和 ( 1 北京协和医学院药用植物研究所, 北京 100193; 2 中草药物质基础与资源利用教育部重点实验室, 北京