1991.,,. 1. ------, ------ 2. ------ ------, 3 ------ ------ 4 ------, ------ 5 ------. PH ------ 6 rrna,45s rrna ------, ------ ---- 7 ------ ATP ------ ------ rrna ------ 8 ------ ------- ------ ------ ------ 9 ------ ------ ------ ------ 10 ------ ------ ------ 11 DNA ------ ------ ------ 12 ------ ------ ------ 13 ------ ------ ------ ------ 14 ------ ------ ------ 15 ------ ------ 1 hnrna 2 mrna 3 DNA = = L 1 1 2 2 20 A-T 10 A X B SOUTHERN C D E F G Northern H I J DNA K L Western Q R S T DNA 1 DNA mrna 2 DNA 3 4 rdna rdna 5 6 G1 S 7 DNA DNA 8 DNA 9-10 1992, 1, 2, 3, 4, 5, 6, 7,, 1, ATP,ATP 2,, 1
3,,,,,. 4,,,,. 5,,. 6,,. 7,,,. 8,rRNA ( ),,. 9, 3400A DNA, 10, mrna,,. 11, 12 13 14 Na+-K+ 15 DNA RNA 16 Hela rrna E. Coli, rdna Hela ( ) 1, 2, 1994, Signal Sequence and Signal patch, 1, 2, 3,,. 4,,,. 5,,. 6,,, 7,, 8, 70nm,, 9,, 10,,, 11,, 2N, 4N,,, 12,,,,, RNA., rrna rdna 1996,,,,, 2
1, 2,,,, ( ),,, 1,,, Hayflick 2, 1997, stem sell,, tonoplast, cell coat, G protein linked receptor, part icle gun bombardment gap junction, Hayflick limit, ( ) 1, cdna,, 2, cdna,, 1,,,,, 2, ( )?, 3,, 4, 5, 1999 1 ---- 2 ---- 3 ---- ---- 4 ---- 5 ---- ---- 6 ---- 7 ---- ---- 8 ---- 9 DNA ---- 10 ---- ---- 1 2 Hayflick 3 4 5 OO 2 456 40 4 1 laser scanning confocal microscopy 2 functional genomics 3 collagen 3
4 G G protein-linked receptor 5 photophosphorylation 6 DNA microsatellite DNA 7 cell cycle 8 kinetochore 9 cdc cell division cycle gene 10 transdifferentiation 60 12 1 2 3 / 4 5 DNA 2001--A. (2/20) 1. 2. 3. 4. 5. 6. 7.ES 8. 9. 10.Hayflick. (1/20) 1..( ) 2.,.( 3..( ) 4.. Ca++, Ca++.( ) 5..( ) 6.,.( ) 7..( ) 8.,.( ) 9.a-.( ) 10..( ) 11. ATG.( ) 12.,.( ) 13. RNA.( ) 14.,.( ) 15. DNA,.( ) 16. 16,.( ) 17..( ) 18.,.( ) 19..( ) 20..( ). (1/20) 1., : A B C D 2.Wilson,. A B C D 3.,. A.Southern blot B.Northern blot C.Western blot D. 4.. 1A B C D 4
5.ATP (F0-F1ATPase). A B C D 6.,. A Na+ B K+ C Ca++ D ATP 7. H+. A 5 B 10 C 50 D 100 8.. A B C D 9.. A B C tau D 2 10.. A B C D 11.,. A DNA B RNA C D 12. cdc2. A.cdc2 B.cdc25 C.cdc28 D.cdc20 13.. A.G1 B.S C.G2 D.M 14.. A B C D 15.. A5'' -GU, 3'' -AG B 5'' -AG, 3'' -GU C 5'' -GA, 3'' -UG D 5'' -UG, 3'' -GA 16.. A RNA B RNA C DNA DNA D DNA DNA 17.. A GTP B C D 18.. A DNA B DNA C D 19.. A B C D 20.,. A B C D. (40) 1.,?? 2.,. 3.. 4.,. 2001B 1.,? 2.? 3.. 4.,, 1997 0 5 30 5
1,,... 2,,,. 3,,.,, ;, 4,,,, 5,,, 1 6,,, 4 8 bp DNA 7,,. 8 MHC. 9 N- O- 10 G1 S S,G1 ;G2 S S,G2 ;G1,S G2 M G1 S G2 11 III 12,,. 13,,. 14 15,,. 1 15 1 EFs PC 1 2 3 4 2 1) 9+2 2 9+0 3 9+2 9+0 4) 9+0 9+2 3 1) 2 3 4 4 Z-DNA P-DNA 1)S 2 G2 3 4 5 1) 2 3 4 6 1) 2 3 4 7 PSII 1)EFu 2 EFs 3 PFu 4 PFs 8 1) 2 3 4 9 1)actin 2 spectrin 3 4 a- 10 1) 2 3 4 11 1) 2 3 4 6
12 G1 1) 2 3 4 13 1)H1 2 H2A 3 H3 4 H4 14 1)G1 2 S 3 G2 4 M 15 1) 2 3 4 0 5 5 1 recombination nodule ( ) 2 microtrobeculae lattice ( ) 3 telomere ( ) 4 Ig ( )5 pachytene ( ) 6 7 8 9 10 2 20 1 2 3 4 5 6 7 8 9 10 1 G 2 3 1998 0 5 30 1 9,,,,,,, 2,, 3 2 3, 4,,,,, 5,,, 6 7-8 6,,,,, 7,, 8,,, 9 10 6,,,, 11,, 12YAC,, 4 20 1 Ribosome Ribozyme 2 3 4 5 MTOC NOR 1 15 1 a b c d N- 2 K+ a b 7
c d 3 a 35 b 27 c 14 d 6 4 a DNA b DNA c DNA d DNA 5 a DNA b c d 6 a b c DNA d RNA 7 a b c d B- 8 a b c d 9 a PCR b Southern blot c Western blot d in situ hybridization 10 a 0.2nm b 2nm c 20nm d 2um 11 5kb a b c d h- 12 a b c d 13 a b c d 14 a b c d 15 FeS a H+ b c H+ d 1 10 1 1 2 3 4 SC 5 6cAMP 7 B- T- 8 9 DNA DNA 10 25 1 8 2 7 8
3 10 2000. (1/30) 1. 19 L- L/D- 20.( ) 2., 67nm( ) 3. DNA GC,Tm ( ) 4. a- Glu, poly(glu) a- ( ) 5. ( ) 6. ( ) 7. DNA ( ) 8., ( ) 9. RNA DNA, ( ) 10. RNA ( ) 11. ( ) 12. ph8, SDS 0.5 ( ) 13. ( ) 14. Anfinsen ( ) 15. a- ( ) 16. ( ) 17., ( ) 18. ( ) 19. ( ) 20., ( ) 21. ( ) 22. BMR ( ) 23. (P/O) ATP ( ) 24.,, ( ) 25. D-, ( ) 26. ( ) 27. HDL ( ) 28. ( ) 29. ( ) 30. THFA ( ). (40 ) 1. N 1~3 C 2. 3. DNA Cot 4., 5. Pribnow box, 6. DNA 1mM EDTA, 7. 8. DNA 300, cm 9.,, 10. 11. Vmax,Km 12. 13. 7.5nm,. 9
14., 15., Asp 16., 17. C1, 18. B6 19., 20., 21., 22., 23. 24.. (30 ) 1. (6 ) 1).Banting 2)Tiselius 3)E.Fisch 4)Calvin 5)Sutherland 6)Gilbert 2.,,,.(6 ) 3.? 4. " "?? 5., Edman,,,, ;, -------[,,, ]. 280nm,,, (6 ) 2001. 1. telomere, centromere 2. unit membrane, biological membrane3. mitosis, meiosis 4. protein kinase A, protein kinase C 5. 6.. 1. 2. 3. 4. 5. 20 1 20 " " "+" " " "-" 1 (+) 2 (+) 3 (-) 4 G G (-) 5 (+) 6 (extracellular matrix) (-) 7 (-) 8 ATP (+) 9 (-) 10 (+) 11 (-) 12 (-) 13 ---- (bacteriorhodopsin) ATP ADP 10
ATP (+) 14 (nuclear localization signal) (+) 15 G1/S G2/M (chekpoint) (+) 16 1% (+) 17 (-) 18 (Leucine-Zipper motif) Jun fos DNA (-) 19 (+) 20 (+) 20 1 20 1 (B) A B C D 2 (D) A B B D 3 (A) A B C D 4 CDK (A) A Well B. CAK C.cdc25 D.p21 5 (lamellipodia) (B) A B C D 6 IV (D) A NADH B C C D C 7 (C) A EGF B PDGF C TGF D IGF-1 8 (B) A G1 B S; C G2; D M 9 (C) A B C D 10 (C) A 9 B 11 C13 D 15 11 (A) A B C D 12 (motor protein) (D) A tau B (actin); C (myosin); D (kinesin) 13 (B) A B C D 14 DNA (D) A B C D S 15 (C) A B rdna C G2 D M S 16 (A) A H2A, H2B,H3, H4 11
B H2A,H2B 4 C H3,H4 4 D H2A, H2B,H3,H4 1 4 17 (B) A B C D 18 (A) A Rb;; B Jun;; C Ras;; D fos;; 19 (C) A B C D 20 (B) A B C D 7 4 28 1 2 3 GTP (GTPase-acivating protein,gap) 4 (position effect) 5 N N 6 DNA 7 " " A A A 4 8 32 1 (MPF) MPF 2 ( ) 3 4 1998. (3 / ) 1. 2. 3.G 4. 5. 6. 7. 8. 9. 10.Hayflick (3 / ) 1.. A. B.ATP C. D. 2.. A. B. C. D. 3.. A. B. C. D. 4.ATP. A. B. C. D. 5.. 12
A. B. C. D. - 6.. A.G1 B.S C.M D.G2 7.hnRNA. A. B. C. D. 8.. A.c-fos B.v-jun C.v-raf D.p53 9.. A. B. C. D. 10.. A.DNA B.DNA C.70S rrna D.80S rrna. (8 / ) 1.? 2.. 3.. 4.. 5.. 1999 0.5 30 1 2 3 4 5 6 ATP 14 4 20 1 2 Receptor mediated endocytosis 3 4 Endosome 5 10 50 * 1 5 ** 3 7 1 2 3 4 5 6 13
7 2000 0.5 20 1 2 3 4 5 6 7 8 9 10 Nuolear lamina 3 30 1 Cadherin 2 Apoptosis 3 Ribosome 4 Microsome 5 6 7 8 9 10 10 50 1 5 2 6 1 2 2 3 MPF maturation-promoting factor G2/M 4 5 6 99. (4 / ) 1. (oridative phosphorylation system) 2. (proteasome) 3. (basol body) 4. (adhesion plague) 5. (cadherin) 6. (nuclear pore complex)7. (cell transfection)8. (nucleosome) 9. (Restriction point of cell cycle)10. (embryonic induction) (10 / ) 1.? 2.?. 3.. 4.. 5.MPF(M )? 6. 99 (1 /, 25 ) 1.,,. 2.,. 3.,, ; ( src ),. 4., N-, 14, 7 25,3 16,4 10.,. 5., I, II 14
. 6.,,. 7.,,. 8.,,. 9.,. 10.,. 11... 12.,,, I,, I,. 13. DNase I,, 3,. 14.,,,,. 15.,. 16. DNA DNA,. 17. M, S. 18,, X,,. 19.,,. 20.,,. 2000 ( 1 25 ) 1 2 1 3 4 5 C- PkkKRKV 6 7 I B563 I 8 9 10 11 200bp DNA 1 1 12 B 13 I 14 SPf S S 15 16 D ( 5 30 ) 1 (phagocytosis) 2 (hyaluronic acid) 15
3 (microbody) 4 (protooncogene) 5 (Polar plasm) 6 flagellum( ) ( 45 ) 1 1883 Ringer 100 Ca2+ 20 Ca2+ camp Ca2+ Ca2+ Ca2+ Ca2+ 2 ( ) 3 Griffith D-looP-t-loop 20 ( cell,97 419-422 1999) 1999 DNA?? 1-2 ( ) Dloop DNA displasmentloop tloop telomere loop 2001 4 1. (autoradiography) 2. (glycophorin) 3. (fluid mosaic model) 4. (microsome) 5. (membrane associated cytoskeleton) 6. (maturation promoting factor,mpf) 7. (nuclearlocalization signal,nls) 8. (zinc finger motif) 9. (synaptonemal complex,sc) 10. (stem cell) 10 10 10 10 20 2001-2002 1. 2. 3. 4. DNA DNA 5. 6. RGD Arg-Gly-Asp 7. 8. 9. mrna SD 10. PIP2 11. 12. 13. 16
14. 15. c-gmp 16. 2. 3. 4. 5. 6. Na-K 8. Ca+ 9. 11. Ca2+ PKA PKC 12. 5SrRNA RNA 13. 4.5% 14. 15. 18. RNA 20. 1. 2. 3. RNA DNA 4. D- D- N-?L- ***** 5. 6. 7. 8. RNA 10. 11. cgmp 12. rrna 17. 19. 20. K+ 1. 2. 3. 4. 5. 26SrRNA RNA 6. 17
1. 2. RNA 5SrRNA 3. 1. " " Ca2+ IP3 Ca2+ Ca2+ 1. 2. 3. N C 96. (2/40) 1. 2. 3. 4. 5. (MPF) 6. Hela 7. IL-2 8. 9. 10. G0 11. 12. 13. 14. G 15. 16. 17. 18. 19. 20.. (10/60) 1. 2.?? 3.. 4.? 5.?? 6.. 97.??(10). Gs (15).?(10). (EGF)???(15)..(10). (4/40) 1. 2. 3. B 4. 5. 6. 7. 8. 9. 10. IL-2 98. " "??(20). I?(20).????(20).?(20). (2/20) 1. 2. 3. SCE(sister chromatid exchange) 4. 5. 6. G- 7. 8. G0 9. 10. 99.??(20).,?(20).???(20) 18
. MPF???(20). (2/20) 1. (NK) 2. ( ) 3. 4. 5. 6. 7. G 8. 9. 10. 2000.,??(15). Ras,?ras?(15).??.(20). DNA??(10). (4/40) 1. SRP(signal recognition particle) 2. PHA 3. G-banding 4. Cyclin 5. B cromosome 6. Ligand 7. Molecular chaperone 8. Telomerase 9. C-oncogene 10. Bibalent 1 20 1 plasma membrane cell membrane, 2 3 70S 80S RNA S S RNA S 4 ATP 5 6 7 Na+ K ATP Na+ K+ 8 1 RNA telomrase 2 3 MAP1 MAP2 MAP4 tau 4 5 Zine finger motif a a helix turn helix motif Leucine Zipper motiy 6 7 H+ ATP 2 H+ 1 ATP 8 NORs DNA rrna 28s 5.8s,18s 5 s rrna 9 10 Microbody microsome ( ) 1 10 1 A B C D 2 A B C D 3 A B C D A.B C 4 N A B C D 5 19
A B C D 6 A B C D 7 A B C D 8 A Tubulin ; B Actin C Keratin D Myosin 9 A B C D 10 A B C D........ 10. 11. DNA 12. 13. 14. 15. 30 1 12 2 8 3 10 2001--A. (2/20) 1. 2. 3. 4. 5. 6. 7.ES 8. 9. 10.Hayflick. (1/20) 1..( ) 2.,.( 3..( ) 4.. Ca++, Ca++.( ) 5..( ) 6.,.( ) 7..( ) 8.,.( ) 9.a-.( ) 10..( ) 11. ATG.( ) 12.,.( ) 13. RNA.( ) 14.,.( ) 15. DNA,.( ) 16. 16,.( ) 17..( ) 18.,.( ) 19..( ) 20..( ). (1/20) 1., : A B C D 2.Wilson,. 20
A B C D 3.,. A.Southern blot B.Northern blot C.Western blot D. 4.. 1A B C D 5.ATP (F0-F1ATPase). A B C D 6.,. A Na+ B K+ C Ca++ D ATP 7. H+. A 5 B 10 C 50 D 100 8.. A B C D 9.. A B C tau D 2 10.. A B C D 11.,. A DNA B RNA C D 12. cdc2. A.cdc2 B.cdc25 C.cdc28 D.cdc20 13.. A.G1 B.S C.G2 D.M 14.. A B C D 15.. A5' -GU, 3' -AG B 5' -AG, 3' -GU C 5' -GA, 3' -UG D 5' -UG, 3' -GA 16.. A RNA B RNA C DNA DNA D DNA DNA 17.. A GTP B C D 18.. A DNA B DNA C D 19.. A B C D 20.,. A B C D. (40) 1.,?? 2.,. 3.. 4.,. 2001B 21
1.,? 2.? 3.. 4.,,. 2002 B " " 20 1 20 " " "+" " " "-" 1 (+) 2 (+) 3 (-) 4 G G (-) 5 (+) 6 (extracellular matrix) (-) 7 (-) 8 ATP (+) 9 (-) 10 (+) 11 (-) 12 (-) 13 ---- (bacteriorhodopsin) ATP ADP ATP (+) 14 (nuclear localization signal) (+) 15 G1/S G2/M (chekpoint) (+) 16 1% (+)! 17 (-) 18 (Leucine-Zipper motif) Jun fos DNA (-) 19 (+) 20 (+) 20 1 20 1 (B) A B C D 2 (D) A B B D 3 (A) A B C D 4 CDK (A) A Well B. CAK C.cdc25 D.p21 5 (lamellipodia) (B) A B C D 6 IV (D) A NADH B C C D C 7 (C) A EGF B PDGF 22
C TGF D IGF-1 8 (B) A G1 B S; C G2; D M 9 (C) A B C D 10 (C) A 9 B 11 C13 D 15 11 (A) A B C D 12 (motor protein) (D) A tau B (actin); C (myosin); D (kinesin) 13 (B) A B C D 14 DNA (D) A B C D S 15 (C) A B rdna C G2 D M S 16 (A) A H2A, H2B,H3, H4 B H2A,H2B 4 C H3,H4 4 D H2A, H2B,H3,H4 1 4 17 (B) A B C D 18 (A) A Rb;; B Jun;; C Ras;; D fos;; 19 (C) A B C D 20 (B) A B C D 7 4 28 1 2 3 GTP (GTPase-acivating protein,gap) 4 (position effect) 5 N N 23
6 DNA 7 " " A A A 4 8 32 1 (MPF) MPF 2 ( ) 3 4 2003 1.5 30 http://yii.533.net 1 2 3 4 5 6. 7 8-6- M6P 9 Western botting RNA 10 mrna RNA 11 12 13 14 15. 16. CDK 17. 18. 19. DNA RNA 20. 1.5 45 http://yii.533.net 1 ] 2 A B C D 2 ----- A B C D 3 ----- A B C G D 4 ----- A camp B DG C IP3 D NO 24
5. A B C D G 6. A B C D 7. A B C D 8. A B C D 9. A N B C D 10. A B C D 11. A B C D 12. A B C D 13. A B RNA C D 5 25 http://yii.533.net 1 2 3 3000000000 H2A H1 4 CDK cyclin-dependent kinase) 5 10 50 http://yii.533.net 1 300 2 3 cdna DNA cdna 25KDa 70KDa 4 500 5 2001--A. (2/20) 1. 2. 3. 4. 5. 6. 7.ES 8. 9. 10.Hayflick. (1/20) 1..( ) 2.,.( 3..( ) 4.. Ca++, Ca++.( ) 5..( ) 6.,.( ) 25
7..( ) 8.,.( ) 9.a-.( ) 10..( ) 11. ATG.( ) 12.,.( ) 13. RNA.( ) 14.,.( ) 15. DNA,.( ) 16. 16,.( ) 17..( ) 18.,.( ) 19..( ) 20..( ). (1/20) 1., : A B C D 2.Wilson,. A B C D 3.,. A.Southern blot B.Northern blot C.Western blot D. 4.. 1A B C D 5.ATP (F0-F1ATPase). A B C D 6.,. A Na+ B K+ C Ca++ D ATP 7. H+. A 5 B 10 C 50 D 100 8.. A B C D 9.. A B C tau D 2 10.. A B C D 11.,. A DNA B RNA C D 12. cdc2. A.cdc2 B.cdc25 C.cdc28 D.cdc20 13.. A.G1 B.S C.G2 D.M 14.. A B C D 15.. A5' -GU, 3' -AG B 5' -AG, 3' -GU C 5' -GA, 3' -UG D 5' -UG, 3' -GA 16.. 26
A RNA B RNA C DNA DNA D DNA DNA 17.. A GTP B C D 18.. A DNA B DNA C D 19.. A B C D 20.,. A B C D. (40) 1.,?? 2.,. 3.. 4.,. 2001B 1.,? 2.? 3.. 4.,,. 2002 B " " 20 1 20 " " "+" " " "-" 1 (+) 2 (+) 3 (-) 4 G G (-) 5 (+) 6 (extracellular matrix) (-) 7 (-) 8 ATP (+) 9 (-) 10 (+) 11 (-) 12 (-) 13 ---- (bacteriorhodopsin) ATP ADP ATP (+) 14 (nuclear localization signal) (+) 15 G1/S G2/M (chekpoint) (+) 16 1% (+)! 17 (-) 18 (Leucine-Zipper motif) Jun fos DNA (-) 19 (+) 20 (+) 27
20 1 20 1 (B) A B C D 2 (D) A B B D 3 (A) A B C D 4 CDK (A) A Well B. CAK C.cdc25 D.p21 5 (lamellipodia) (B) A B C D 6 IV (D) A NADH B C C D C 7 (C) A EGF B PDGF C TGF D IGF-1 8 (B) A G1 B S; C G2; D M 9 (C) A B C D 10 (C) A 9 B 11 C13 D 15 11 (A) A B C D 12 (motor protein) (D) A tau B (actin);c (myosin); D (kinesin) 13 (B) A B C D 14 DNA (D) A B C D S 15 (C) A B rdna C G2 D M S 16 (A) A H2A, H2B,H3, H4 B H2A,H2B 4 C H3,H4 4 D H2A, H2B,H3,H4 1 4 17 (B) A B C D 18 (A) A Rb;; B Jun;; C Ras;; D fos;; 19 (C) 28
A B C D 20 (B) A B C D 7 4 28 1 2 3 GTP (GTPase-acivating protein,gap) 4 (position effect) 5 N N 6 DNA 7 " " A A A 4 8 32 1 (MPF) MPF 2 ( ) 3 4 2003 1.5 30 http://yii.533.net 1 2 3 4 5 6. 7 8-6- M6P 9 Western botting RNA 10 mrna RNA 11 12 13 14 15. 29
16. CDK 17. 18. 19. DNA RNA 20. 1.5 45 http://yii.533.net 1 A B C D 2 ----- A B C D 3 ----- A B C G D 4 ----- A camp B DG C IP3 D NO 5. A B C D G 6. A B C D 7. A B C D 8. A B C D 9. A N B C D 10. A B C D 11. A B C D 12. A B C D 13. A B RNA C D 14. 30 5 25 http://yii.533.net 1 2 3 3000000000 H2A H1 4 CDK cyclin-dependent kinase) 30
5 10 50 http://yii.533.net 1 300 2 3 cdna DNA cdna 25KDa 70KDa 4 500 5 2001--A. (2/20) 1. 2. 3. 4. 5. 6. 7.ES 8. 9. 10.Hayflick. (1/20) 1..( ) 2.,.( 3..( ) 4.. Ca++, Ca++.( ) 5..( ) 6.,.( ) 7..( ) 8.,.( ) 9.a-.( ) 10..( ) 11. ATG.( ) 12.,.( ) 13. RNA.( ) 14.,.( ) 15. DNA,.( ) 16. 16,.( ) 17..( ) 18.,.( ) 19..( ) 20..( ). (1/20) 1., : A B C D 2.Wilson,. A B C D 3.,. A.Southern blot B.Northern blot C.Western blot D. 4.. 1A B C D 5.ATP (F0-F1ATPase). A B C D 6.,. A Na+ B K+ C Ca++ D ATP 7. H+. 31
A 5 B 10 C 50 D 100 8.. A B C D 9.. A B C tau D 2 10.. A B C D 11.,. A DNA B RNA C D 12. cdc2. A.cdc2 B.cdc25 C.cdc28 D.cdc20 13.. A.G1 B.S C.G2 D.M 14.. A B C D 15.. A5' -GU, 3' -AG B 5' -AG, 3' -GU C 5' -GA, 3' -UG D 5' -UG, 3' -GA 16.. A RNA B RNA C DNA DNA D DNA DNA 17.. A GTP B C D 18.. A DNA B DNA C D 19.. A B C D 20.,. A B C D. (40) 1.,?? 2.,. 3.. 4.,. 2001B 1.,? 2.? 3.. 4.,,. 2002 20 1 20 " " "+" " " "-" 1 (+) 2 (+) 3 (-) 4 G G (-) 5 (+) 32
6 (extracellular matrix) (-) 7 (-) 8 ATP (+) 9 (-) 10 (+) 11 (-) 12 (-) 13 ---- (bacteriorhodopsin) ATP ADP ATP (+) 14 (nuclear localization signal) (+) 15 G1/S G2/M (chekpoint) (+) 16 1% (+)! 17 (-) 18 (Leucine-Zipper motif) Jun fos DNA (-) 19 (+) 20 (+) 20 1 20 1 (B) A B C D 2 (D) A B B D 3 (A) A B C D 4 CDK (A) A Well B. CAK C.cdc25 D.p21 5 (lamellipodia) (B) A B C D 6 IV (D) A NADH B C C D C 7 (C) A EGF B PDGF C TGF D IGF-1 8 (B) A G1 B S; C G2; D M 9 (C) A B C D 10 (C) A 9 B 11 C13 D 15 11 (A) A B C D 12 (motor protein) (D) A tau B (actin); C (myosin); D (kinesin) 33
13 (B) A B C D 14 DNA (D) A B C D S 15 (C) A B rdna C G2 D M S 16 (A) A H2A, H2B,H3, H4 B H2A,H2B 4 C H3,H4 4 D H2A, H2B,H3,H4 1 4 17 (B) A B C D 18 (A) A Rb;; B Jun;; C Ras;; D fos;; 19 (C) A B C D 20 (B) A B C D 7 4 28 1 2 3 GTP (GTPase-acivating protein,gap) 4 (position effect) 5 N N 6 DNA 7 " " A A A 4 8 32 34
1 (MPF) MPF 2 ( ) 3 4 2003 1 2 3 4 5 6. 7 8-6- M6P 9 Western botting RNA 10 mrna RNA 11 12 13 14 15. 16. CDK 17. 18. 19. DNA RNA 20. 1.5 45 http://yii.533.net 1 A B C D 2 ----- A B C D 3 ----- A B C G D 4 ----- A camp B DG C IP3 D NO 5. A B C D G 6. A B C D 7. A B C D 8. A B C D 9. A N B C D 35
10. A B C D 11. A B C D 12. A B C D 13. A B RNA C D 5 25 http://yii.533.net 1 2 3 3000000000 H2A H1 4 CDK cyclin-dependent kinase) 5 10 50 http://yii.533.net 1 300 2 3 cdna DNA cdna 25KDa 70KDa 4 500 5 36