3 5 Vol. 3 No. 5 2012 10 Journal of Food Safety and Quality Oct., 2012 林 洪 *, 曲 欣 (, 266003) 摘 要 :,,, 关 键 词 : ; ; The research progress of purine distribution in food LIN Hong *, QV Xin (Food Science and Engineering College, Ocean University of China, Qingdao 266003, China) ABSTRACT: The determination methods of purine are not unified nowadays, so the results of purine detection are discrepant in some foods. In this paper, methods of sample preparation, purine quantification and purine distribution in food were reviewed and analyzed to provide a reference for further development of purine detection technologies. It has an important significance in guiding consumers to a rational diet so that the incidence of gout could be decreased. KEY WORDS: purine; gout; determination 1 引 言, 20%,,, [1, 2], 50% [1],, 2009 16.99%, 1.36%, 2004 3.72% 0.26% [3, 4] [5, 6],,,, [7],,, 2 食 品 中 嘌 呤 的 检 测 方 法 2.1 样 品 前 处 理 方 法,,,, 基 金 项 目 : (31271844) * 通 讯 作 者 :,,, : E-mail: linhong@ouc.edu.cn
374 3 [8] [9], 2.1.1 酸 解 提 取 法,,,, (Adenine) (Guanine); (Hypoxanthine), ;, (1 1, v/v), [10] ;,, ; (1 1, v/v) (Xanthine), (5 5 1, v/v/v),, 90 12 min, 4,, [11-13],,, 2.1.2 超 声 波 提 取 法,, [14-16], [17],,,,,, 2.1.3 有 机 溶 剂 萃 取 法, Clariana [18] 6 ( ); [19] 75%,,,,, 2.2 食 品 中 嘌 呤 的 定 量 方 法 50, 1947 1948 Vischer Hotchkiss, 2000 [19],, 89%, 5%,,,,,, [20, 21] [22], (HPLC) C 18 C 18, C 18, 1,, C 18,, C 18, (RP-ICP); dc 18 RP 18 C 18, 0.02 mol/l, ph, 254 nm, 0.6~1.0 ml/min, 24~30 3 食 品 中 嘌 呤 含 量 分 布,,, Clifford [28],,
5, : 375 Table 1 表 1 色 谱 柱 在 嘌 呤 检 测 中 的 选 用 情 况 The selection of chromatographic column in purine determination Kromasil-C 18 Hyp a : (1:9, v/v) HPLC Alltech Apollo C 18 Hyp : (1:9, v/v) HPLC Kromasil-C 18 Xan b Hyp : (2:98, v/v) HPLC Discovery C 18 Hyp :(8:92, v/v) HPLC μbondapak C 18 Atlantis C 18 Ade c Gua d Ade Gua Ade Gua Waters symmetryshield RP 18 Waters Atlantis dc 18 Ade Gua Waters Atlantis dc 18 : a: ; b: ; c: ; d: Ade Gua :: : (883.5: 100:15:1.5, v/v/v/v) [ : : (997:1.5: 1.5), v/v/v]:(9:1, v/v) (0.02 mol/l, ph 4.6) (0.02 mol/l, ph 4) (0.02 mol/l, ph 3.8) RP-ICP RP-ICP HPLC HPLC HPLC [14] [15] [23] [16] [24] [25] [26] [27],, 3.1 畜 禽 肉 类 中 嘌 呤 含 量 分 布 50%,,, 1977, [29] 50 [13, 30], 2,,, [30], 10 min,,,,, (ATP), 3.2 水 产 品 中 嘌 呤 含 量 分 布,, 2, ; A B, ;,, ; 18 6,,,, /,,, ATP (IMP) [11] 60%, 10 min, 10 min,,,, [31-33, 35],,,,,
376 3 表 2 部 分 食 品 中 嘌 呤 含 量 Table 2 Contents of purine in some foodstuffs (mg/kg) 140 190 40 474 844 232 324 39 363 958 523 788 48 324 1684 770 1182 211 22 2186 220 150-656 1027 162 135 43 580 920 624 1021 232 160 2036 212 268 23 516 1019 148 84 60 565 857 130 107 56 366 659 159 119 33 723 1034 190 182 50 540 962 8230 6990 77 388 15685 4950 560-1680 7190 113 72 15 601 801 A 87 87 29 665 868 B 119 492 21 610 1242 142 372 13 817 1344 693 557 373 252 1875 1640 195 4 96 1935 1197 182 1 90 1470 [30] [30] [31] [32] [33] [33] 3.3 大 豆 中 嘌 呤 含 量 分 布 1982, [36] 743.1 982.2 0.0 0.0 mg/kg, 1725.3 mg/kg Kaneko K [37] HPLC, 219~1725 mg/kg mg/l [38] 732.3 821.2 20.2 31.8 mg/kg, 1605.5 mg/kg,, 3.4 酒 精 饮 料 中 嘌 呤 含 量 分 布 [39],,, [39, 40], [24] 1 14.1 33.6 17.3 2.1 mg/l, 2 3 [25] 14 [10] 17.24 309.68 18.06 5.09 mg/l,, 0.82 0.67 0.62 mg/l, HPLC, [22] LC-MS/MS,,,, [27],, 3.5 其 他 食 品 中 嘌 呤 含 量 分 布
5, : 377,, [19] [41], 4 小 结,,,,,, 参 考 文 献 [1] Nuki G. Gout[J]. Medicine, 2002, 30(9): 71 77. [2] Wall GC, Cooper SA. Gout and Hyperuricemia: New Guidelines and Treatments [J]. J Pharm Pract, 2009, 22(1): 104 115. [3],,,. [J]., 2006, 22(5): 421 425. [4],,,. [J]., 2011, 27(7): 548 552. [5] Sundstrom J, Sullivan L, D'Agostino RB, et al. Relations of serum uric acid to longitudinal blood pressure tracking and hypertension incidence[j]. Hypertension, 2005, 45(1): 28-33. [6] Iseki K, Ikemiya Y, Inoue T, et al. Significance of hyperuricemia as a risk factor for developing ESRD in a screened cohort[j]. Am J Kidney Dis, 2004, 44(4): 642 650. [7]. [M]. :, 2009. [8] Smolenski R, Lachno D, Ledingham S, et al. Determination of sixteen nucleotides, nucleosides and bases using high-performance liquid chromatography and its application to the study of purine metabolism in hearts for transplantation[j]. J Chromatogr, 1990, 527(2): 414 420. [9]. [D]., 2011. [10],,. HPLC [J]., 2008, (2): 76 79. [11],. [J]., 1997, 24(1): 1 11. [12] Fan H, Yang FQ, Li SP. Determination of purine and pyrimidine bases in natural and cultured Cordyceps using optimum acid hydrolysis followed by high performance liquid chromatography[j]. J Pharmaceut Biomed, 2007, 45(1): 141 144.,,,. 4 [J]., 2008, 36(6): 724 728. [14],. HPLC [J]., 2006, 20(7): 408 409. [15]. HPLC [J]., 2007, 34(12): 1783. [16],, 喆,. HPLC [J]., 2005, 28(8): 670 672. [17],,,. HPLC [J]., 2002, 25(10): 716 717. [18] Clariana M, Gratacós-Cubarsí M, Hortós M, et al. Analysis of seven purines and pyrimidines in pork meat products by ultra high performance liquid chromatography-tandem mass spectrometry[j]. J Chromatogr A, 2010, 1217(26): 4294 4299. [19],,,. [J]., 2000, 21(5): 70 72. [20] Chen G, Chu Q, Zhang L, et al. Separation of six purine bases by capillary electrophoresis with electrochemical detection[j]. Anal Chim Acta, 2002, 457(2): 225 233. [21] Xu D, Hua L, Chen H. Determination of purine bases by capillary zone electrophoresis with wall-jet amperometric detection[j]. Anal Chim Acta, 1996, 335(1 2):95 101. [22],,,. LC-MS/MS [J]., 2010, 26(12): 1399-1402. [23],,,. HPLC [J]., 2002, 25(10): 71 717. [24],,,. [J]., 2006, (9): 219 222. [25],. [J]., 2009, 07: 146 150. [26]. [D]., 2009. [27],. [J]., 2006, 32(3): 73 75. [28] Clifford AJ, Riumallo JA, Young VR, et al. Effect of Oral Purines on Serum and Urinary Uric Acid of Normal, Hyperuricemic and Gouty Humans [J]. J Nutr, 1976, 106(3): 428 434. [29],, 龍. 製 塩 [J]. J Jpn Soc Nutr Food Sci, 1977, 30(3): 155 162. [30],,,. [J]., 2008, 29(7): 67 69. [31] Piñeiro-Sotelo M, Rodrı guez-bernaldo De Quirós A, López-Hernández J, et al. Determination of purine bases in sea urchin (Paracentortus lividus) gonads by high-performance liquid chromatography[j]. Food Chem, 2002, 79(1): 113 117. [32] Lou S. Purine Content in Grass Shrimp during Storage as Related
378 3 to Freshness[J]. J Food Sci, 1998, 63(3): 442 444. [33],,,. [J]., 2008, 33(11): 112 115.,,,. [J]., 2012, 34(1): 74 78. [35] Lou S, Chen H, Hsu P, et al. Changes in purine content of tilapia surimi products during processing[j]. Fisheries Sci, 2005, 71(4): 889 895. [36] 篠,, 龍. 塩 対 調 響 [J]. 栄 栄 糧, 1982, 35: 103 109. [37] Kaneko K, Kudo Y, Yamanobe T, et al. Purine contents of soybean-derived foods and selected Japanese vegetables and mushrooms[j]. Nucleosides Nucleotides Nucleic Acids, 2008, 27(6): 628 630. [38]. [D]., 2009. [39] Yamamoto T, Moriwaki Y, Takahashi S. Effect of ethanol on metabolism of purine bases (hypoxanthine, xanthine, and uric acid)[j]. Clin Chim Acta, 2005, 356(1 2): 35 57. [40] Choi HK, Atkinson K, Karlson EW, et al. Alcohol intake and risk of incident gout in men: a prospective study[j]. The Lancet, 2004, 363(9417): 1277 1281. [41] Yamaoka N, Kaneko K, Kudo Y, et al. Analysis of purine in purine-rich cauliflower[j]. Nucleosides Nucleotides Nucleic Acids, 2010, 29(4-6): 518 521. ( 责 任 编 辑 : 赵 静 ) 作 者 简 介 林 洪,,, : E-mail: linhong@ouc.edu.cn