9291-3122002 Ann. Rept. NIEA Taiwan R.O.C. 9291-3122002 Ann. Rept. NIEA - 40 107 6 19 40 54.2 4.87 µg/l0.89-50.0 µg/l 77.4 7.54 µg/l1.08-50.0 µg/l 6 74 2,625 µg/kg dry wt250-8,580 µg/ kg dry wt 8.21-12.22 µg/ml 8 5.38-75.36 µg/ml 46 2001,Vol.20,No.3:202-215. 291
Residues Survey of Nonylphenol and Its Biological Effect on Male Carp Objectives:The purpose of this research was to survey the residual nonylphenol, one of the endocrine disruptors, in rivers and sediments in Taiwan.The potential endocrine disrupting effect on male carp was also investigated. MethodsA total of 107 water samples from 40 rivers and 19 sediment samples from 6 rivers in Taiwan were collected for measuring nonylphenol using capillary GC/MSD. USEPA 525.2 method was adapted for treating water samples and the ROC-EPA method NIEA R815.20B for sediment samples. As for the fish assay: male carps were exposed to nonylphenol for two weeks and followed up for four weeks. Vitellogenin in fish plasma was examined using enzyme-linked immunosorbent assay (ELISA) for the evaluation of biological endocrine disruption effect. Results: Nonylphenol was detected in 54.2% water samples with an average concentration of 4.87 µg/l, in the range of 0.89 to 50.0µg/L. The detectable rate was the highest for rivers of southern Taiwan (77.4%) with an average concentration of 7.54 µg/l, in the range of 1.08 to 50.0 µg/l. In the sediment of six rivers, the chemical was found in 74% samples with an average concentration 2625 µg/kg dry wt, in the range of 250 to 8580 µg/kg dry wt. The detected concentrations varied among rivers and among different sections in a river. For fishes that exposed to water with 40 µg /L nonylphenol, vitellogenin in study fish plasma, ranged from 8.21 to 12.2 µg/ml was about eight times higher than that of controls, by two-week follow-up. After four weeks,the vitellogenin contents rised to 5.38-75.4 µg/ml. For fishes fed with nonylphenol,the vitellogenin level was 46-times higher than for control group. Conclusion: These data suggest that nonylphenol in river water and commercial detergents has a potential harmful impact on the environment and public health in Taiwan. Key words: Endocrine disrupting substance, Environmental hormone, Nonylphenol 292
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NP NP (µg/l) (µg/l) 0/5 0/2 2/2 2/3 0 0 1.01 8.64 --- --- 1.01-1.02 3.87-13.4 3/3 19.4 1.76-33.8 7/7 2/3 1/3 3.60 4.94 1.39 2.02-6.07 4.70-5.18 1.39 2/3 10.0 3.04-17.0 0/4 1/1 0/3 2/3 0 0.89 0 3.08 --- 0.89 --- 2.60-3.55 13 52.4 5.89 0.89-33.8 0/3 0 --- 0/5 0 --- 1/6 0/1 1/1 1/1 1/2 0/2 2.01 0 3.23 1.90 5.31 0 2.01 --- 3.23 1.90 5.31 --- 2/3 11.1 1.32-20.9 9 25.0 4.71 1.32-20.9 3/3 3/3 1/2 2/3 3/4 1/1 2/3 1/1 0/2 3/3 3/4 2/2 4.48 1.57 7.93 1.74 2.23 1.75 7.01 50.0 0 1.63 3.0 1.57 1.42-10.24 1.08-2.29 7.93 1.66-1.82 1.69-2.66 1.75 1.47-12.6 50.0 --- 1.55-1.76 1.94-4.44 1.40-1.73 12 77.4 7.54 1.08-50.0 1/1 1/2 2/2 1/2 1/1 0/2 1.37 1.21 1.50 1.49 1.29 0 1.37 1.21 1.32-1.67 1.49 1.29 --- 6 66.7 1.37 1.21-1.67 40 54.2 4.87 0.89-50.0 306
Ann. Rept. NIEA NP NP µg/kg (µg/kg dry wt) dry wt 2/4 345 330-360 2/5 520 490-550 3/3 330 250-390 4/4 517 250-1190 2/2 7400 6220-8580 1/1 6590 6590 6 74% 2625 250-8580 307
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