PCR Polymerases Dress Code go 讓 PCR 實驗用最適合的聚合酶! PCR Polymerase! Best in-class enzymes Expert scientific support Broad application 免付費客服專線

PCR Polymerases Dress Code 您的 PCR 實驗穿對衣服了嗎?,,,! General Purpose High Yield High Fidelity Long & GC-rich Fast Direct dress code GO03

PCR Polymerases Selection Guide Polymerase Cat. # Amplification Efficiency Product Size λdna Recommended / Max Product Size Human Genomic DNA Recommended / Max Fidelity Proofreading Activity Specificity GC-Rich Templates Hot-Start PCR Dye Added Processing Speed (per/kb) TA Cloning (3'-A overhang) General Purpose Polymerases TaKaRa Taq TM DNA Polymerase R001A ++ 6 kb/12 kb 2 kb/4 kb 1 X Taq** X ++ + X X 1 min Premix Taq TM DNA Polymerase R004A ++ 6 kb/12 kb 2 kb/4 kb 1 X Taq** X ++ + X X 1 min TaKaRa Taq TM DNA Polymerase HS R007A ++ 6 kb/12 kb 2 kb/4 kb 1 X Taq** X ++++ + X 1 min Premix Taq TM DNA Polymerase HS R028A ++ 6 kb/12 kb 2 kb/4 kb 1 X Taq** X ++++ + X 1 min EmeraldAmp GT PCR Master Mix RR310A ++ 6 kb/12 kb 2 kb/4 kb 1 X Taq** X ++ +++ X 1 min EmeraldAmp MAX PCR Master Mix RR320A ++++ 10 kb/20 kb 4 kb/10 kb 3 X Taq** ++ ++++ X 1 min High Yield Polymerases EmeraldAmp MAX HS PCR Master Mix RR330A ++++ 10 kb/20 kb 4 kb/10 kb 3 X Taq** ++++ ++++ 1 min TaKaRa Ex Taq DNA Polymerase RR001A ++++ 20 kb/30 kb 10 kb/20 kb 4.5 X Taq** ++ + X X 1 min Premix Ex Taq TM DNA Polymerase RR003A ++++ 20 kb/30 kb 10 kb/20 kb 4.5 X Taq** ++ + X X 1 min TaKaRa Ex Taq DNA Polymerase HS RR006A ++++ 20 kb/30 kb 10 kb/20 kb 4.5 X Taq** ++++ + X 1 min Premix Ex Taq DNA Polymerase HS RR030A ++++ 20 kb/30 kb 10 kb/20 kb 4.5 X Taq** ++++ + X 1 min Titanium Taq DNA Polymerase* 639208 ++++ up to 2 kb up to 2 kb 1 X Taq** X ++++ ++ X 1 min Advantage 2 Polymerase Mix* 639201 ++++ up to 18.5 kb up tp 6 kb 3 X Taq** ++++ ++ X 1 min High Fidelity Polymerases CloneAmp TM HiFi PCR Premix 639298 +++ up to 15 kb up to 6 kb ~12 errors/ 542,580 bp # ++++ +++ X 5 sec X (Blunt) PrimeSTAR GXL DNA Polymerase R050A +++ up to 40 kb up to 30 kb ~1 error/16, 230 bp # ++++ ++++ X 10 sec X (Blunt) PrimeSTAR Max DNA Polymerase R045A ++++ up to 15 kb up to 6 kb ~1 error/45, 177 bp # ++++ +++ X 5-10 sec X (Blunt) Long PCR & GC-rich PCR Polymerases TaKaRa LA Taq DNA Polymerase RR002A +++ 35 kb/48 kb 20 kb/30 kb 6.5 X Taq** ++ + X X 1 min TaKaRa LA Taq DNA Polymerase HS RR042A +++ 35 kb/48 kb 20 kb/30 kb 6.5 X Taq** ++++ + X 1 min TaKaRa LA Taq DNA Polymerase w/gc Buffers RR02AG +++ 35 kb/48 kb 20 kb/30 kb 6.5 X Taq** ++ ++++ X X 1 min PrimeSTAR GXL DNA Polymerase R050A +++ up to 40 kb up to 30 kb ~1 error/16, 230 bp # ++++ ++++ X 10 sec X (Blunt) Advantage GC 2 Polymerase Mix 639114 +++ up to 6 kb up to 3.5 kb 3 X Taq** ++++ ++++ X 1 min Fast PCR Polymerases SapphireAmp Fast PCR Master Mix RR350A ++++ 10 kb/20 kb 4 kb/10 kb 3 X Taq** ++++ ++ 10 sec SpeedSTAR TM HS DNA Polymerase RR070A +++ 20 kb/30 kb 10 kb/20 kb 4.5 X Taq** ++++ + X 10 sec PrimeSTAR Max DNA Polymerase R045A ++++ up to 15 kb up to 6 kb ~1 error/45, 177 bp # ++++ +++ X 5-10 sec X (Blunt) ++++ Best * All of DNA Polymerases are provided with dntps except for Titanium Taq and Advantage 2 +++ Good When used with GC Buffer I ++ Average **Fidelity determined by using the Kunkel method + Poor #Fidelity determined by direct sequencing 04

Dress Code General Purpose Polymerases TaKaRa Taq TM DNA Polymerase Multiplexing DNA Length (Avg/Max) : 6kb/12kb DNA T/A Cloning Premix Hot start EmeraldAmp GT PCR Master Mix 2X Premix DNA Polymerase! Loading dye,pcr, Genotyping GC-rich AT-rich PCR, T/A Cloning DNA B e c a u s e t y p i c a l l y Ta q D N A polymerase is cloned in E. coli, it is especially important for bacterial amplifications to test Taq polymerase for the presence of contaminating E. coli genomic DNA. EmeraldAmp MAX PCR Master Mix 10 Taq Taq 3 X 3-5kb PCR Dye ~50bp Cat. # Product Size R001A/B TaKaRa Taq TM DNA Polymerase 250 Units / 1000 Units RR310A/B EmeraldAmp GT PCR Master Mix 160 Rxns / 800 Rxns RR320A/B EmeraldAmp MAX PCR Master Mix 160 Rxns / 800 Rxns 05

Dress Code High Yield Polymerases EmeraldAmp MAX HS PCR Master Mix TaKaRa Ex Taq DNA Polymerase 2X Premix DNA Polymerase! Loading dye,pcr, GC-rich AT-rich PCR, T/A Cloning Hot start : primer dimer Low Copy Number and/or Dirty Samples, (4.5X Taq) DNA Length (Avg/Max) : 20kb/30kb DNA T/A Cloning Premix Hot start 3 EmeraldAmp GT EmeraldAmp Max EmeraldAmp Max HS Routine PCR Fast PCR Colony PCR GC/ AT Rich Hot start Max length (Avg/ Max) 6K/12K 10K/20K 10K/20K Yield ++ ++++ ++++ Proofreading (0.01ng) template false-negative Cat. # Product Size RR330A EmeraldAmp MAX HS PCR Master Mix 160 Rxns RR330B EmeraldAmp MAX HS PCR Master Mix 800 Rxns RR001A TaKaRa Ex Taq DNA Polymerase 250 Units RR001B TaKaRa Ex Taq DNA Polymerase 1000 Units 06

Titanium Taq TM DNA Polymerase Mammalian Genotyping N,,! 2 copies, Hot-Start : annealing, multiplexing primer PCR 10 Activation,! T/A Cloning Affymetrix TITANIUM? Advantage 2 Polymerase Mix Titanium Proofreading Proofreading, 3-6 SMART Optimized PCR, 20 Kb Hot-Start : annealing, multiplexing primer PCR 10 Activation,! T/A Cloning 20KB Clontech TATANIUM Taq,, Affymetrix, SNP GeneChip Assay TITANIUM Dilute? RACE Lane 1: 2.5-kb E. co l i DNA polymerase gene amplified from genomic DNA. Lane 2: 3.5-kb bovine pancreatic trypsin inhibitor gene amplified from calf thymus genomic DNA. Lane 3: 5.9-kb human IL-1βgene fragment amplified from human genomic DNA. Lane 4 : 8.5-kb human titin cdna amplified from a human skeletal muscle SMART cdna librar y. Lane 5: 18.5-kb inser t amplified from a clone. Lane M: /Hind III DNA size markers. Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cdna with Advantage 2 Polymerase Mix and a competitor`s Taq polymerase mix. 5 µl of PCR products were run on a 1.1% agarose/etbr gel. Lane 1: The 0.4-kb TNFR II fragment is readily obtained with Advantage 2. Lane 2: No product is seen with Taq polymerase. Lane M: DNA size marker. Units, TITANIUM Taq Units, 25 units ( dilution ) Cat. # Product Size 639208 Titanium Taq DNA Polymerase 100 Rxns (2500 Units) 639209 Titanium Taq DNA Polymerase 500 Rxns (125,00 Units) 639201 Advantage 2 Polymerase Mix 100 Rxns 639202 Advantage 2 Polymerase Mix 5 100 Rxns 07

Dress Code High Fidelity Polymerases GC-rich templates excess template Long PCR product CloneAmp TM HiFi PCR Premix PrimeSTAR GXL DNA Polymerase : ~12 errors/ 542,580 bp : priming extension (5 sec/kb) : (up to 10 kb) GC-rich Hot start : Hot start antibody, :2X master mix buffer dntps :In-Fusion Cloning Proofreading, 3-6 SMART Optimized PCR, 20 Kb Hot-Start : annealing, multiplexing primer PCR 10 Activation,!! Mutation frequency of CloneAmp HiFi Polymerase compared to other high-fidelity PCR enzymes. Highly Specific Amplification of Long Products - PrimeSTAR GXL Outperforms Other Enzymes. 0.5 1 2 4 6 8.5 12 15 18 20 0.5 1 2 4 6 8.5 12 15 Eight arbitrarily selected GC-rich regions were amplified with CloneAmp HiFi Polymerase or other DNA polymerases using a Thermus thermophilus HB8 genomic DNA template, and cloned into suitable plasmids. Multiple clones were selected for each amplification product and subjected to sequence analysis. DNA fragments amplified using CloneAmp HiFi Polymerase yielded only 12 mismatched bases per 542,580 total bases higher than an alternative highfidelity enzyme from Company A, and 10-fold higher than Taq DNA polymerase. 0.5 1 2 4 6 8.5 12 15 18 20 0.5 1 2 4 6 8.5 12 15 18 20 Excellent results - no need for special buffers or reaction conditions. Products amplified from human genomic DNA. Cat. # Product Size 639298 CloneAmp TM HiFi PCR Premix 200 Rxns R050A PrimeSTAR GXL DNA Polymerase 250 Rxns R050B PrimeSTAR GXL DNA Polymerase 1000 Rxns M. λ-hind III 1. p53 0.5 kb 2. DCLRE1A 1 kb 3. DCLRE1A 2 kb 4. DCLRE1A 4 kb 5. p53 6 kb 6. β-globin 8.5 kb 7. β-globin 12 kb 8. β-globin 15 kb 9. β-globin 18 kb 10. β-globin 20 kb 08

PrimeSTAR Max DNA Polymerase :, PCR (~1 error/45,177 bp) :5-10 sec / kb Hot start : Hot start antibody, PrimeSTAR MAX! :2X Premix ( buffer dntps) : DNA Sequencing, Cloning and Expression PrimeSTAR Max Template: Human genomic DNA 0,5 1 2 3 4 6 7,5 kb Extension time : 10 seconds PrimeSTAR Max can amplify human genomic DNA up to6 kb in length in 30 seconds PrimeSTAR MAX PCR,! Ten arbitrarily selected GC-rich regions were amplified with various DNA polymerases, using Thermus thermophilus HB8 genomic DNA as template. Each PCR product (approx. 500 bp each) was cloned into a suitable plasmid. Multiple clones were selected per respective amplification and were subjected to sequence analysis. Error rate expressed as % of sequenced nucleotides. 0.5 1 2 4 6 8 10 12 0.5 1 2 4 6 8 10 12 0.5 1 2 4 6 8 10 12 Cat. # Product Size R045A PrimeSTAR MAX DNA Polymerase 100 Rxns R045B PrimeSTAR MAX DNA Polymerase 400 Rxns Template : λ DNA 0.5, 1, 2, 4, 6, 8, 10, 12 kbp. Extension time : 10 seconds. PrimeSTAR Max can amplify λ DNA up to 6 kb in length in 30 seconds/cycle. 09

Dress Code Long PCR & GC-rich PCR Polymerases LONG LONG GC TaKaRa LA Taq DNA Polymerase TaKaRa LA Taq DNA Polymerase with GC Buffers! DNA Length (Avg/Max) : 35kb/48kb Taq, 6.5X! Long PCR, Sequencing Cloning T/A Cloning Hot Start, 74% GC content secondary structure! GC Buffer: GC Buffer I: amplification of fragments 5 kb GC Buffer II: 2 3 kb fragments with up to 73% GC content. High GC,!,! Amplification of a Huntington's Disease Gene (high GC content) Template : Human genomic DNA Target : HD gene IT15 CAG repeat fragment sizes 262 bp (GC content 73%) and 358 bp (GC content 71.5%) Template Amount : 100 ng in 50 µl reaction Template amplified with : A : LA Taq polymerase+ LA PCR Buffer II B : LA Taq polymerase + GC Buffer I C : LA Taq polymerase + GC Buffer II D : Competing DNA polymerase and high GC Kit M : 100 bp ladder Amplification of regions of β-globulin and TPA genes(long PCR product) Template : Purified human genomic DNA Template Amount : 500 ng in 50 µl reaction Cat. # Product Size RR002A TaKaRa LA Taq DNA Polymerase (Mg 2+ free buffer) 125 Units RR02AG TaKaRa LA Taq DNA Polymerase with GC Buffer 125 Units 10

LONG GC PrimeSTAR GXL DNA Polymerase : 30 kb DNA :,~1 error/16,230 bp : GC/ AT rich, Hot start : Hot start antibody, Kit: buffer dntps : Long PCR, Sequencing, Cloning, Amplification Prior to NGS GC Advantage GC 2 Polymerase Mix, GC-rich templates 6 kb DNA PCR Kit: dntps Mix, Control Template & Primer PCR-Grade Water 80% GC content! PrimeSTAR GXL DNA Polymerase amplifies products up to 30 kb (human genomic DNA template), 40 kb (lambda DNA), or 13.5 kb (human cdna). Lane M: DNA size marker Lane 1: Advantage 2 + 6% DMSO + 0.1 M GC-Melt Lane 2: Advantage 2 + 1.0 M GC-Melt Lane 3: Advantage GC 2 + 1.0 M GC-Melt Lane 4: Advantage 2 + 5% DMSO A 510 bp portion of IGFR II cdna, containing a 110 bp region with a GC content of 90%, was amplified from Human Placenta Marathon-Ready cdna (Cat. No. 639311) Cat. # Product Size R050A/B PrimeSTAR GXL DNA Polymerase 250 / 1000 Rxns 639114 Advantage GC 2 Polymerase Mix 100 Rxns 11

Dress Code Fast PCR Polymerases SapphireAmp Fast PCR Master Mix PCR:2 kb 1 Loading dye,pcr : Taq 3 X :PCR, T/A Cloning 2X Premix, ddh 2 O, Hot start antibody: primer dimer Colony PCR! 2 kb 1 hr OK Colony PCR : 12

SpeedSTAR TM HS DNA Polymerase 30 2 kb (extension rate: 10 sec/kb) : PCR machine optimized buffers: 20 kb T/A Cloning Hot start antibody: primer dimmer PrimeSTAR Max DNA Polymerase :5-10 sec / kb :, PCR (~1 error/45,177 bp) Hot start : Hot start antibody, :2X Premix ( buffer dntps) : DNA Sequencing, Cloning and Expression Template : cdna [equivalent to 100 ng total RNA) / 50µl reaction] PCR conditions 98 10 sec. 55 15 sec. 72 10 or 30 sec. 30 cycles Good amplification was observed for products up to 2 kb in length using an elongation time of 10 sec and for products up to 4 kb in length using an extension time of 30 sec. Amplification of Human Genomic DNA Targets of Varying Sizes using SpeedSTAR High speed amplification is especially valuable when amplifying large-size targets. This data shows excellent specificity and yield. Cat. # Product Size RR350A/B SapphireAmp Fast PCR Master Mix 160 Rxns / 800 Rxns RR070A/B SpeedSTAR TM HS DNA Polymerase 250 Units / 1000 Units R045A/B PrimeSTAR MAX DNA Polymerase 100 Rxns / 400 Rxns 13

Dress Code Terra TM PCR Direct Add tissues, plants, blood DIRECTLY into the PCR reaction! Direct PCR Polymerases DNA! : FFPE samples Whatman FTA cards Hot start version : primer dimmer >70% GC-content, 2 kb T/A cloning 95 for 10 min Neutralization Genomes Amplified Using Terra PCR Direct : Human Mouse Drosophila C. elegans Xenopus Yeast/Mycelia Zebrafish Cow Sheep Chicken Lamb Pig Corn Rice Arabidopsis Sugar cane Cotton Soybean Mosquito Trichogramma Dragonfly H. pylori Bacillus Fusarium Moss sporophytes Chestnut Arugula 14

Amplify directly from a variety of tissues. cat no Product Size Terra PCR Direct polymerase Mix 639287 Terra PCR Direct Card Kit 200 Rxns Whatman FTA cards 1.2-2.0 mm diameter disc 639284 Terra PCR Direct FFPE Kit 200 Rxns FFPE samples 1-1.5 cm 2 639285 Terra PCR Direct Genotyping Kit 200 Rxns 639270 200 Rxns 639271 Terra PCR Direct Polymerase Mix 800 Rxns 639286 Terra PCR Direct Red Dye Premix 200 Rxns Mouse tail biopsies : 1mm Mouse ear biopsiies : 2mm 2 disc Mouse organs : 1.5mm 3 Blood: 5ul Mouse tail biopsies : 1mm Mouse ear biopsiies : 1.5mm 2 Plant leaves : 1.2mm 2 disc 15

Cat.no 639270 639271 639286 639285 639284 639287 Terra PCR Direct Solution Features Components Polymerase Mix Red Dye Premix Genotyping Kit FFPE Kit FTA Card Kit Premix with convenient loading All-in-one kit; most robust performance Application-specific kit Application-specific kit Buffer [Mg2+, dntps] Buffer Buffer [Mg2+, dntps] Buffer [Mg2+, dntps] Buffer [Mg2+, dntps] Polymerase Mix [Mg2+, dntps] Polymerase Mix Polymerase Mix Polymerase Mix Polymerase Mix Loading Dye Loading Dye Extraction Buffer* Extraction Buffer Proteinase K Proteinase K Proteinase K Proteinase K Recommended Sample Types : FFPE X Blood (FTA) X Blood (Heparin) X X X Plant Leaves (FTA) X Plant leaves X X X* Buccal cells (FTA) X Buccal cells X X X Saliva X X X Tissues X X X* Cell pellet X X X Crude extract X X X Purified DNA X X X * The Terra PCR Direct Genotyping Kit includes an extraction buffer, which we recommended using tough plants or tissue samples. 16

Quick-gDNA TM Kit 75 gdna,15 min : :15 total DNA Proteinase K organic denaturants : EDTA, heparin, citrate DNA/RNA Shield DNA PCR endonuclease digestion bisulfite conversion sequencing genotyping DNA Quick-gDNA TM MicroPrep 1x10 6 cells, 5 mg tissues, 50 µl whole blood 5 µg 6 µl D3020 50 rxns D3021 200 rxns Quick-gDNA TM MiniPrep 5x10 6 cells, 25 mg tissues, 200 µl whole blood 25 µg 30 µl D3024 50 rxns D3025 200 rxns Quick-gDNA TM MidiPrep 25x10 6 cells, 100 mg tissues, 3 ml whole blood 125 µg 150 µl D3100 25 rxns 17

75 ZR Plasmid Miniprep TM - Classic DNA:plasmid DNA endotoxin-free, transformation cloning transfection in vitro transcription : buffer, Zymo column : buffer Zymo-Spin TM Column Q Spin Column buffer, Restriction Endonuclease digestion of three different plasmids prepared using the ZR Plasmid Miniprep - Classic, performed in triplicate. Bacterial Culture Input D4015 100 rxns ZR Plasmid Miniprep TM - Classic 0.5-5 ml 25 µg 30 µl D4016 400 rxns D4054 800 rxns 18

PCR Polymerases Dress Code PCR! 20153/31 Premix PCR Enzymes 藍寶石綠寶石 85 Titanium Taq Advantage 2 8 PCR Polymerase TaKaRa Taq Ex Taq LA Taq 10 Quick-gDNA TM Kit ZR Plasmid Miniprep TM - Classic P.17~18 75 Terra PCR Direct PrimeSTAR Terra PCR Direct TEL (02)2720-2215 TEL (05)2844-162 TEL (03)6684-5 8 6 TEL (06)2890-665 TEL (04)2463-3591 TEL (07)3470-143 免付費客服專線 U031128