Chinese Journal of Applied Entomology 2015, 52(5): 1149 1153. DOI: 10.7679/j.issn.2095 1353.2015.136 联苯菊酯和啶虫脒对苹果黄蚜的 * 区分剂量研究 封云涛 ** 刘中芳 王 1 振张润祥庾琴 *** 高越范仁俊 1. 030031 2. 030031 摘要 目的 Aphis citricola 方法 结果 0.021 μg/cm 2 0.003 μg/cm 2 4 20 d 结论 关键词 Research on discrimination dose of acetamiprid and bifenthrin in Aphis citricola van der Goot FENG Yun-Tao ** WANG Zhen 1 ZHANG Run-Xiang YU Qin LIU Zhong-Fang GAO Yue *** FAN Ren-Jun (1. Institute of Plant Protection, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China; 2. Shanxi Key Laboratory of Integrated Pest Management in Agriculture, Taiyuan 030031, China) Abstract [Objectives] In order to quickly and easily determine the susceptibility of Aphis citricola van der Goot to conventional insecticides, the discrimination dose of acetamiprid and bifenthrin was determined. [Methods] It was used discriminating dose bioassay on the basis of improved diagnostic dose method, and was verified with filed populations. [Results] The results showed that the susceptive discrimination dose values for bifenthrin and acetamiprid were 0.021 μg/cm 2 and 0.003 μg/cm 2, respectively. Storage test showed that the membrane of acetamiprid and bifenthrin can be stored up to 20 days at 4. [Conclusion] The test results reproducibility is better, and it can be used for rapid detection of sensitivity to acetamiprid and bifenthrin. Key words Aphis citricola van der Goot, discrimination dose, acetamiprid, bifenthrin, sensitivity detection, storage time Aphis citricola van der Goot 2005 * Supported projects 200903033 2015ZZCX-15 ** First author E-mail fyt52@126.com *** Corresponding author E-mail rjfan@163.com Received 2014-09-30 Accepted 2015-03-04
1150 Chinese Journal of Applied Entomology 52 LD-p 99% Plutella xylostella L. 19962005 2005 20052012 Phyllotreta striolata Fabricius2006 Aphis gossypii Glover1990 Helicoverpa armigera Hübner2000 2000 Leptinotarsa decemlineata Say2010 Apolygus lucorum Meyer-Dür 2012 Nilaparvata lugens Stål2011 LC 99 LD 99 99% 1 材料与方法 1.1 试虫 Aphis citricola 5.69 3.01 1.2 供试药剂和仪器 98.54% 95.60% 7.5 cm 2.5 cmolympus SZX7 1.3 方法 1.3.1 毒力测定 7.5 cm 2.5 cm 500 µl 20 4 cm 31.40 cm 2 25±1 14 L: 10 D 4 5~8 1 h 3 SPSS Probit 1.3.2 区分剂量确定 LC 50 LC 60 LC 70 LC 80 LC 90 1.3.1 4 1.3.3 验证实验 1.3.4 储存时间对毒力测定的影响 4
5 : 1151 2 结果与分析 2.1 毒力测定 1 1 LC 50 2.2 区分剂量确定 50% 60% 70% 80% 90% 2 1.3 mg/l 0.19 mg/l LC 99 LD 99 1.3 mg/l 0.19 mg/l 0.021 μg/cm 2 0.003 μg/cm 2 2.3 验证实验 3 Insecticides 表 1 苹果黄蚜对联苯菊酯和啶虫脒的敏感性 ( 药膜法 ) Table 1 The susceptibility of Aphis citricola to bifenthrin and acetamiprid Repeats Regression equation R 2 LC 50 mg/l 95% 95%FLmg/L 1 y=5.750+1.217x 0.962 0.242 0.090-0.427 Bifenthrin Acetamiprid 2 y=5.563+0.967x 0.906 0.262 0.008-0.904 3 y=5.589+1.373x 0.967 0.372 0.206-0.645 Combine y=5.688+1.346x 0.956 0.308 0.204-0.425 1 y=6.778+1.550x 0.931 0.071 0.030-0.117 2 y=5.970+0.970x 0.941 0.100 0.037-0.209 3 y=5.705+0.688x 0.982 0.094 0.009-0.271 Combine y=5.832+0.801x 0.927 0.092 0.048-0.156 Insecticides Bifenthrin Acetamiprid 表 2 联苯菊酯和啶虫脒不同剂量下苹果黄蚜的死亡率 Table 2 Mortality of Aphis citricola to different dosage of bifenthrin and acetamiprid Theory mortality (%) Concentration (mg/l) Dosage (μg/cm 2 ) Adjusted mortality (%) 50 0.308 0.0049 11.560 1.580 60 0.475 0.0076 65.391 2.630 70 0.755 0.0120 57.461 3.091 80 1.300 0.0021 79.650 2.052 90 2.758 0.0439 82.050 2.650 50 0.092 0.0015 46.790 1.690 60 0.190 0.0030 57.250 2.880 70 0.413 0.0066 77.510 1.560 80 1.028 0.0164 96.950 2.341 90 3.638 0.0579 98.660 1.643
1152 Chinese Journal of Applied Entomology 52 表 3 药剂区分剂量对不同品系苹果黄蚜毒力 Table 3 Toxicity of discrimination dose to Aphis citricola Insecticides Insects Mortality (%) Mortality of CK (%) Adjusted mortality (%) Field strain 11.631 2.13 4.880 3.211 7.100 0.750 Bifenthrin Susceptible strain 79.751 3.18 3.700 1.114 78.970 2.871 Field strain 28.890 1.89 5.380 2.052 24.850 3.610 Acetamiprid Susceptible strain 56.520 2.10 2.530 0.894 55.390 2.062 3 5.69 3.01 2.4 不同储存时间对毒力测定的影响 1 4 3 7 10 20 d 60% 图 1 不同储存时间对毒力测定的影响 Fig. 1 The effect of different storage time to toxicity determination 20% 4 20 d 3 结论 LC 99 LD 99 15 20 3 5 1 h 60% 80%
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