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1 4 5 Vol. 4 No Journal of Food Safety and Quality Oct., 2013 王超 1, 周勇 2, 杜冰 1* (1., ; 2. ( ), ) 摘要 : 目的方法 SD,, (Hb) (FEP) 结果 4,, (P<0.05), FEP (P<0.01);, (P<0.05), FEP (P<0.01), ; 结论关键词 : ; ; ; ; Evaluation on extracts from Lentinula edodes and Auricularia auricula-judae in improving nutritional anemia in rats WANG Chao 1, ZHOU Yong 2, DU Bing 1* (1. College of Food Science, South China Agricultural University, Guangzhou , China; 2. Infinitus(China) Co., Ltd., Jiangmen , China) ABSTRACT: Objective To study the effect of extracts from Lentinula edodes (LE) and Auricularia auricula-judae (AA) on improving nutritional anemia. Methods The SD rats were made into nutritional anemia model by feeding iron deficient diet, then the rats were randomly divided into low iron model group, LE extract gavage group, AA extract gavage group, ferrous lactate control group and normal control group, and the body weight, food intake, content of hemoglobin (Hb), red blood cell free protoporphyrin (FEP) content, and changes of hematocrit of each group were analyzed. Results After 4 weeks, when compared with low iron model group, the rats of LE group and AA group had significant increases (P<0.05) in body weight, food intake, hemoglobin content and hematocrit, while having significant decreases (P<0.01) in FEP content. When compared with ferrous lactate group, the rats of middle and high dose group had significant increases (P< 0.05) in hemoglobin content and hematocrit and significant decreases (P<0.01) in FEP content. In addition, the body weight, food intake increased slightly. Besides, the indicators of extract gavage group were close to normal group. Conclusion The LE and AA extracts could significantly improve the symptoms of nutritional anemia rats. KEY WORDS: Lentinula edodes; Auricularia auricula-judae; extraction; nutritional anemia; rats * 通讯作者 :,, [email protected] *Corresponding author: DU Bing, Associate Professor, College of Food Science, South China Agricultural University, No.483, Wushan Road, Tianhe District, Guangzhou , China. [email protected]

2 1388 4,, D,, [1,2],, [3-5], [6,7], [8,9],,, [10],,,,, [11],,,, 1 材料与方法 1.1 受试物与受试对象 :, ( ) (, 1: 25, 6 h, 70 [12,13] ) : SD, SPF, 40~60 g, 1.2 仪器和试剂, : Milli-Q/B( Millipore); Mek-7222k ( ); BS-3000A ( ); ELX800 ( BIO-TEX); FT-630ǐ- ( ) : (FEP)ELISA ( ); ( ); ( ) 1.3 实验方法 : 10, ; 80, [14] : (Hb)<100 g/l, Hb A A A B B B, 10 / [15], A B 1 ml (A B), 2 ml(a B), 3 ml (A B) (, 1.5 ml), 3 ml; 3 ml (, 1.5 ml); 3 ml (, 1.5 ml);, 30 d 1.4 观察及检测指标体态及行为, 体重, 摄食量, 1, g / d -1, (g/ d -1 )=[ (g) (g)]/ ( ) [16,17] 血红蛋白含量, 10 μl, 2.5 ml,,, 15 min,, 红细胞内游离原卟啉含量, 4,, 2%, 4, : (4:1), 10 min, 0.5 mol/l,, ELISA 红细胞压积 9 μl,,, 2 min, [18]

3 5, : 统计分析 Hb ( x ±, SPSS 结果与分析 2.1 受试样品 A B 对缺铁性贫血大鼠模型日常的影响, ( ),,, A B 2.2 受试样品 A B 对缺铁性贫血大鼠模型体重的影响 1,,,,, (P<0.01) 2.3 受试样品 A B 对治疗期间缺铁性贫血大鼠模型摄食量影响 2,,,, ;, A, ; 表 1 A B 对治疗期间缺铁性贫血大鼠模型体重的影响 ( x± Table 1 Effect of A, B on body weight of iron deficiency anemia rat model during treatment ( x± (g/ ) n ( ) ** ** ** ** ** * * ** A * ** ** A * * ** ** A * * ** ** B * * ** ** B * * ** ** B * * ** ** 表 2 A B 对治疗期间缺铁性贫血大鼠模型摄食量的影响 ( x± Table 2 Effect of A, B on food intake of iron deficiency anemia rat model during treatment ( x± n ( ) (g/ ) ** ** ** ** * A A * A * * B * B * * ** B * **

4 受试样品 A B 对缺铁性贫血大鼠模型血红蛋白含量红细胞内游离原卟啉值的影响 3,, (P<0.01),,, Hb (P<0.01), FEP A 2.5 受试样品 A B 对缺铁性贫血大鼠模型红细胞压积的影响 4,, (P<0.01),,, 表 3 A B 对缺铁性贫血大鼠模型血红蛋白含量和 FEP 的影响 ( x± Table 3 Effect of A, B on content of hemoglobin and FEP of iron deficiency anemia rat model during treatment ( x± (g/l) n ( ) Hb Hb FEP (μg/dl) ** ** ** ** ** A ** * A ** ** A ** ** B ** * B ** ** B ** ** 表 4 A B 对缺铁性贫血大鼠模型红细胞压积的影响 ( x± Table 4 Effect of A, B on hematocrit of iron deficiency anemia rat model during treatment ( x± (%) n ( ) ** ** ** A * A ** A * ** B * B ** B * **

5 5, : 结论与讨论,, (P<0.05), FEP (P<0.01);, (P<0.05), FEP (P<0.01), ;,, FEP, (Fe 2+ ),,,, FEP,, [19,20] FEP,, [21] [12],,,,, 参考文献 [1],. MJ [J]., 2003, 9(4): Long H, Wang GC. MJ Hemoglobin piece features to improve human nutritional anemia tasting test results analysis [J]. J Prev Med Inform, 2003, 9(4): [2],,. [J]., 2009, 49(29): Lu LH, Sun LL, Fan B. The margin of sheet iron to improve nutritional anemia effect [J]. Shandong Pharm, 2009, 49(29): [3],,,. [J]., 2009, 19(8): Han YB, Peng L, Zhao P, et al. Yunting brand gelatin to improve oral nutritional anemia in rats evaluation [J]. Chin J Health Lab, 2009, 19(8): [4]. [J]., 2004, 12(6): Yin SA. China's growth and development of infants and young children and complementary feeding conditions [J]. Chin J Child Health Care, 2004, 12(6): [5]. [J]., 2002, 36(2): Chinese students' health research group. chinese students anemia dynamic observation [J]. J Prev Med, 2002, 36(2): [6] Lozoff B. Behavioral alterations in iron deficiency [J]. Adv Pediatr, 1988, 35: [7] Loz off B, Jimenez E, Hagen J. et al. Poorer behavioral anddevelopmental outcome more than 10 years after treatment for irondeficiency in infancy [J]. Pediatrics, 2000,105(4): E51. [8] Scholl TO, Hediger ML, Fischer RL, et al. Anemiavs iron deficiency: increased risk of preterm delivery in a prospective study [J]. AmJ Clin Nutr, 1992, 55: [9] Lu ZM, Goldenburg RL, Cliver SP, et al. The relationship between maternal hematocrit and pregnancy outcome [J]. Obstet Cynecol, 1991, 71: [10],,. [J]., 2009, 17(2): Xiao YF, Wang RW, Mei JT. Fortified lunch on improving student observe the effect of iron deficiency anemia [J]. Chin J Child Health Care, 2009, 17(2): [11]. [J]., 2009, 29(7): Zhou J. Nutritional anemia [J]. Chin J Int Med, 2009, 29(7): [12]. [J]., 2005, 32(4): Li YM. Lentinula Endous Research Status and Prospects [J]. J Microbiol, 2005, 32(4): [13],., [J]., 2007, 7(7): Han CR, Xu LP. Auricularia auricular polysaccharides extraction, purification and lipid-lowering effect [J]. Chin Inst Food Sci, 2007, 7(7): [14],,,. SD [J]., 2007, 01: Wei HY, Ma G, Zhang L, et al. Female SD rats model of iron deficiency anemia [J]. Sichuan Anim, 2007, 01: [15],,,.

6 [J]., 2006, (4): Wang HL, Meng J, Wang LL, et al. Iron deficiency anemia rat model and supplement ferrous lactate improve hemoglobin level [J]. Chin J Food Hyg, 2006, (4): [16],,,. [J]., 2006, (3): Yang Z, Luo HJ, Lu XC, et al. Rat model of iron deficiency anemia and various indicators of observational studies [J]. J Trop Med, 2006, (3): [17],,. [J]., 2004, (3): 34. Liu XX, Li C, Li XQ. Iron deficiency anemia rat model the behavior observed body [J]. Chin J Behav Med Sci, 2004, (3): 34. [18],,,. [J]., 2007, 8(7): Zhu JF, Fei W, Ma XH, et al. Gelatin oral iron nutritional anemia improved functional studies [J]. Xinjiang Med Univ, 2007, 8(7): [19]. [J]., 2007, 18(3): 185. Qu HY. Run red rouge oral improving nutritional anemia in rats experimental study [J]. Chin Pharm, 2007, 18(3): 185. [20],,,. [J]., 2007, 30(8): Zhu JF, Fei W, Ma XH, et al. Gelatin oral iron nutritional anemia improved functional studies [J]. Xinjiang Med Univ, 2007, 30(8): [21]. [D]., Liu X. Lentinan myelosuppressed regulation of hematopoietic [D]. Chengdu University of TCM, 作者简介 ( 责任编辑 : 赵静 ) 王超, 硕士研究生, 主要研究方向为食品生物技术 @qq.com 杜冰, 副教授, 主要研究方向为食品生物技术 [email protected] 粮油产品质量安全专题征稿投稿方式 : [email protected] 食品安全质量检测学报编辑部

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