What are the primary bacteria found in semen and does it matter? 猪精液中有哪些主要细菌, 它们是问题么? Dr. Darwin Reicks 达尔文瑞克斯博士 Swine Vet Center St. Peter, MN 56082

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1 What are the primary bacteria found in semen and does it matter? 猪精液中有哪些主要细菌, 它们是问题么? Dr. Darwin Reicks 达尔文瑞克斯博士 Swine Vet Center St. Peter, MN 56082

2 Control bacteria in the extended semen 控制稀释精液内的细菌 Culture every collection day 每个收集日做培养 Streak on last day semen will typically be used 一般使用最后一天的精液上的条纹 Know where you are at 了解自己在哪一步

3 Culture Results SVC 2004 培养结果 - SVC 2004

4 Culture Results SVC 培养结果 SVC

5 Control bacteria in the extended semen 控制扩展精液中的细菌 Best way to control is to minimize the bacteria in raw ejaculate 最好的控制方法是尽量减少原精液中的细菌

6 How to Minimize Bacteria 如何减少细菌 Keep Barn dry 保持谷仓干燥

7 How to Minimize Bacteria Shave Boars Prepuce 剃净公猪包皮如何减少细菌 Electric razor best 最好使用电动剃须

8 How to Minimize Bacteria 如何减少细菌 Wash hands frequently 勤洗手

9 How to Minimize Bacteria 如何减少细菌 Evacuate prepuce 淸包皮四周 Use outer wrist-length glove 使用外部过腕长手套 Wipe with paper towel 用纸巾擦

10 How to Minimize Bacteria 如何减少细菌 Try to have the tip free and clear 尽量让末端干净 Don t rest hand on top of cup 不要将手放在杯子上 Do this Avoid this

in 避免碎片坠入 Cover as soon as possible 尽快覆盖如何减少细菌 Do

11 How to Minimize Bacteria Be aware of ejaculate after collection 请注意采集后的精液射出 Avoid debris falling in 避免碎片坠入 Cover as soon as possible 尽快覆盖如何减少细菌 Do not squeeze filter contents into ejaculate 不要挤压过滤器的内容物到原精液中

12 How to Minimize Bacteria 如何减少细菌 Disinfect dummies at least once/week 至少每周消毒取精器一次 Take off and scrub underside 取下并擦洗底面 No carpet or leather on dummy 不在取精器上放毯子或皮具

13 How to Minimize Bacteria 如何减少细菌 Culture water frequently 经常做水培养

14 How to Minimize Bacteria 如何减少细菌 Reduce clutter in lab 减少实验室中的混乱 Sanitize daily 每日消毒

15 How to Minimize Bacteria 如何减少细菌 Replace all tubing at least once/month 每月至少更换所有管子一次 Wash tubing daily 每日清洗管子 Detergent (contrad 70) 洗涤剂 Water 清水 Alcohol 酒精

16 How to Minimize Bacteria 如何减少细菌 Wash hands with soap and water multiple times per day 每日多次用肥皂和清水洗手 After being in barn 去过猪舍后 Every couple hours while processing 每两个小时 After bathroom breaks 每用过洗手间

17 Sampling 取样 Should be done every collection day 每个采集日都应进行 Culture samples >48 hours old 培养样品 >48 小时

18 Goal: No Growth 目标 : 没有生长

19 Culture Water Source 培养水源

20 Other areas to culture 其他需培养区域

21 When bacteria are found 当发现细菌时 It s most often a single bacteria 经常是单个细菌 Forward to reference lab for identification & sensitivity 转送至参考实验室来鉴别和识别和敏感测试

22 Bacteria Isolate 分离菌 Most Common Isolates from Studs at Swine Vet Center 在猪兽医中心的公猪站中最常见的菌系 44,600 doses cultured 培养 44,600 列 Frequency of isolation 分离频率 Arcanobacterium (Coryn.) species (+) 346 Skin, prepuce Typical source 典型来源 Burkholderia cepacia / sp. (-) 314 Water, soil, environment Stenotrophomonas maltophilia (-) 275 Water, soil, plants, urine, resp tract Pseudomonas (-) 260 Environment / prepuce Staphylcoccus species (+) 215 Skin, prepuce Gram neg non-fermenter (-) 201 Water, soil, environment Strep species (+) 190 Skin, prepuce, mucus, feces Achromobacter (Alcaligenes) xyl. (-) 187 Environment, water Enterococcus (+) 184 Feces/intestine/prepuce Serratia marcescens (-) 173 environment Proteus (-) 160 Water, soil, feces, environ.

25 Case Report 案例 Blood tinged catheter 带血色的导管 Post Insemination Discharge 输精后排出物

26 Case Report Enterococcus toxin 案例 - 肠毒素

27 Serratia Case Report 沙雷氏菌属个案报告 Complaint by boar stud 公猪站投诉 Motility not looking good after Day 3 3 天后精子活力不理想 Bacteria identified in semen 精液中发现细菌 Identification made 做了鉴别

29 Study 研究 Reicks DL, Levis DG. Fertility of semen used in commercial production and the impact of sperm numbers and bacterial counts. Theriogenology 70 (2008), pp 用于商业化生产的精液和精子数量和细菌计数对生育的影响兽医产科学 70 (2008), pp Study of two 30,000 sow systems over 4 years 研究了两个 30,000 母猪体系长达 4 年 Did not find any correlation between presence of bacteria and Farrowing Rate or Total Born 没有发现精液中细菌和分娩率及出生数量之间的直接关系 Depends on which bacteria and how much 取决于什么细菌和多少细菌

30 Summary 总结 Monitor extended semen doses daily 每日监控稀释精液剂量 water source also 和水源 Intervene quickly to reduce losses 迅速处理, 以减少损失 Serratia and Enterococcus toxin positive require urgent interventions 沙雷氏菌和肠球菌毒素阳性, 需要紧急处理 Some bacteria probably won t detect losses 有些细菌可能不会导致检测到的损失 Use semen <5 days of age, regardless of extender 不管稀释液如何, 使用 5 天以内的精液

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What are the primary bacteria found in semen and does it matter? 猪精液中有哪些主要细菌, 它们是问题么? Dr. Darwin Reicks 达尔文 瑞克斯博士 Swine Vet Center St. Peter, MN 56082

What are the primary bacteria found in semen and does it matter? 猪精液中有哪些主要细菌, 它们是问题么? Dr. Darwin Reicks 达尔文瑞克斯博士 Swine Vet Center St. Peter, MN 56082