2 : 541 Jones Pseudaletia separata Harpaz 1969 Spodoptera littoralis Price Ratcliffe Jones 6 Price Jones Jones Pr

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1 Chinese Journal of Applied Entomology 2014, 51(2): DOI: /j.issn 黑翅痂蝗血细胞形态学的研究 * 任彩红 ** 李小玉吴哲张小民 *** 摘要目的方法 Wright-Giemsa Bryodema nigroptera Zheng 结果 8 结论 1% 32.2% 57.3% 1% 6% 1% 1% 0.5% 关键词 Morphology of the hemocytes of Bryodema nigroptera Zheng REN Cai-Hong ** LI Xiao-Yu WU Zhe ZHANG Xiao-Min *** (School of Life Science, Shanxi University, Taiyuan , China) Abstract [Objectives] To describe the characteristics and morphology of the hemocytes of Bryodema nigroptera Zheng and describe the evolutionary relationships between the different types of hemocytes in order to further understanding of the cellular immunology of locusts and provide a basis for locust prevention and control. [Methods] Hemocytes of B. nigroptera were stained with Wright-Giemsa and observed under an optical microscope. [Results] B. nigroptera has 8 types of hemocytes; prohaemocytes, plasmatocytes, granulocytes, oenocytoids, cystocytes, adipohemocytes, vermiform cells and megakaryocytes. [Conclusion] In adults, prohaemocytes accounted for 1%, plasmatocytes for 32.2%, granulocytes for 57.3%, oenocytoids for 1%, Cystocytes for 6%, adipohemocytes for 1%, vermiform cells for 1% and megakaryocytes for 0.5%, of all hemocytes. Granulocytes were the most abundant followed by plasmatocytes, and megakaryocytes were the least abundant. Key words Bryodema nigroptera, hemocytes, morphology 1845 Newport Barratt Arnold1910 Dytiscus marginalis Hydrophilus piceus Mathur Soni1937 Schistocerca gregaria Tauber Griffiths1943 Blattaorientalis Yeager1945 Prodenia eridania Bessis1950 Arnold1952 Mediterranean flour moth 4 Pauly 1955 Gregoire1959 * ** rencaihong8822@126.com *** zhangxm@sxu.edu.cn

2 2 : 541 Jones Pseudaletia separata Harpaz 1969 Spodoptera littoralis Price Ratcliffe Jones 6 Price Jones Jones Prohemocytes 2 Plasmatocytes3 Granular hemocytes4 Cystocytes 5 Spherule cells 6 Oenocytoids7 Adipohemocytes 3 Podocytes Vermiform cells Granulocytophagus Brehelin Laekie 1985 Orthoptera Dictyoptera Azambuja 方法 Luckhart 1992 Simulium vittatum Butt Shields 1995 Lymantria dispar 1998 Australian cockroach Culex quinquefasciatus 5 Chang 2002 Brayner 2005 Culex quinquefasciatus 2007 Catantops pinguis Locusta migratoria manilensis Ademolu 2010 Orthoptera: Pyrgomorphidae 2011 Oxya chinensis Mohammed 黑 Bryodema nigroptera Zheng Wright-GiemsaW-G 黑 1 材料与方法 1. 1 材料 % Wright-GiemsaW-G Olympus BX-51 2 结果与分析

3 542 Chinese Journal of Applied Entomology 51 μm 46 μm W-G (Prohaemocytes) (Plasmatocytes) (Granulocytes) (Oenocytoids) (Cystocytes) (Adipohemocytes) (Vermiform cells) (Megakaryocytes) 原血胞 (Prohaemocytes, PR) 20 μm μm 1% 1A 2.2 浆血胞 (Plasmatocytes, PL) 6% 1F 13 μm 32 μm 2.6 脂血胞 (Adipohemocytes, AD) 818 μm 32.2% 1BC 2.3 粒血胞 (Granulocytes, GR) 15 μm 32 μm 822 μm 1 μm 3 μm 2.8 巨核血细胞 (Megakaryocytes, ME) 57.3% 1D 2.4 类绛色血细胞 (Oenocytoids, OE) 30 μm μm 25 μm~35 10~18 μm 1% 1E 2.5 囊血胞 (Cystocytes, CY) μm~30 μm 45 μm 15~25 μm 13 μm 30 μm 8~15 μm 1% 1G 2.7 蠕形细胞 (Vermiform cells, VE) 7 μm 80 μm 150 μm 6~20 μm 1% 1H

2 : 543 Fig. 1 图 1 黑翅痂蝗血细胞光学显微镜图像 The optical microscope s image of the hemocyte of Bryodema nigroptera Zheng A. Prophemocytes, PR 1 000; B. Proplasmatocytes, PPL 1 000; C. Plasmatocytes, PL 1 000; D.

4 2 : 543 Fig. 1 图 1 黑翅痂蝗血细胞光学显微镜图像 The optical microscope s image of the hemocyte of Bryodema nigroptera Zheng A. Prophemocytes, PR 1 000; B. Proplasmatocytes, PPL 1 000; C. Plasmatocytes, PL 1 000; D. Granulocyte, GR 1 000; E. Oenocytoids, OE 1 000; F. Cystocytes, CY 1 000; Granulocyte, GR 1 000; G. Adipohemocytes, AD 1 000; H. Vermiform cells, VE 1 000; I. Megakaryocytes, ME Scale bar=10 μm.

5 544 Chinese Journal of Applied Entomology % 1I 3 讨论 3.1 染色方法 GiemsaWright s Eosin Hematoxylin Alcian blue Jones1977 Giemsa Wright s Şapcaliu Giemsa Wright s Giemsa Wright s 8 1F~H 3.2 黑翅痂蝗血细胞形态黑 Jones1977 1E 1F Jones a1982b 2007 黑 Wright-Giemsa 3 Wright-Giemsa Giemsa-Wright 黑 1G Jones1977 1% 1 HYeager1945 Jones1977 Ribeiro Brehélin2006

6 2 : 545 黑 μm 1I 3.3 黑翅痂蝗血细胞之间的演化关系 8 Gupta Sutherland a 1% 1A 1 B 1C 32.2% 4 0.5% 1I 57.3% 1D 1% 1H 1% 1E 6% 1F 1% 1G 8 黑 8

7 546 Chinese Journal of Applied Entomology 51 参考文献 (References) Arnold J, The haemocytes of the Mediterranean flour moth, Ephestia kühniella Zell. (Lepidoptera: Pyralididae). Canad. J. Zool., 30(6): Azambuja PD, Garcia ES, Ratcliffe NA, Aspects of classification of Hemiptera hemocytes from six triatomine species. Memorias do Instituto Oswaldo Cruz, 86(1): Ademolu KO, Idowu AB, Olatunde G, Hemocyte populations in Zohocerus variegatus(l.)(orthoptera: Pyrgomorphidae)during post-embryonic development. Acta Entomologica Sinica, 53(4): Barratt JOW, Arnold G, A study of the blood of certain Coleoptera: dytiscus marginalis and Hydrophilus piceus. Quart. J. Micros. Sci., 2(221): Bessis M, Studies in electron microscopy of blood cells. Blood, 5(12): Brehelin M, Zachary D, Hoffmann JA, A comparative ultrastructural study of blood cells from nine insect orders. Cell and Tissue Research, 195(1): Butt TM, Shields KS, The structure and behaviour of gypsy moth (Lymantria dispar) hemocytes. Journal of Invertebrate Pathology, 68(1): Brayner FA, Araujo HRC, Cavalcanti MG, Alves LC, Peixoto CA, Ultrastructural characterization of the hemocytes of Culex quinquefasciatus (Diptera: Culicidae). Micron, 36(4): Chang BS, Yeo SM, Wago H, Kang SK, Han SS, Hematopoiesis in Hematopoietic Organ in Euprepocnemis shirakii (Orthoptera, Insecta). Entomological Research, 32(1): Gregoire C, Recordings of clotted hemolymph of insects by electron microscopy. Arch. Intern. Physiol. Biochim., 67(2): Gupta AP, Sutherland DJ, In vitro transformations of the insect plasmatocyte in some insects. J. Insect Physiol., 12(11): Harpaz I, Kislev N, Zelcer A, Electron-microscopic studies on hemocytes of the Egyptian cottonworm, Spodoptera littoralis (Boisduval) Infected with a nuclear polyhedrosis virus, as compared to noninfected hemocytes. I. Noninfected hemocytes. Journal of Invertebrate Pathology, 14(2): Jones JC, Current concepts concerning insect hemocytes. Amer. Zoologist, 2(1): Jones JC, The Circulatory System of Insects.Charles C &Thomas Publisher Laekie AM, Takle G, Tefley L, Haemocytic encapsulation in the locust Schistocerca gregaria (Orthoptera) and in the cockroach Periplaneta americana (Dictyoptera). Cell and Tissue Research, 240(2): Luckhart S, Cupp MS, Cupp EW, Morphological and functional classification of the hemocytes of adult female Simulium vittatum (Diptera: Simuliidae). J. Med. Entomol., 29(3): Mathur CB, Soni BN, Studies on Schistocerca gregaria Forsk. IX. Some observations on the histology of the blood of the desert locust. Ind. J. Agric. Res., 7: Mohammed IS, Mohammed Saleh Al-Khalifa, Circulating Haemocytes in insects: phylogenic review of their types. Pakistan J. Zool., 44(6): Newport G, On the structure and development of the blood. First series. The development of the blood-corpuscles in insects and other Invertebrata, and its comparison with that of man and the Vertebrata. Ann. Mag. Nat. Hist., 15: Pauly LK, Serological relationships among orthopteroid insects as determined by their whole blood. Journal of the New York Entomological Society, 63: Price CD, Ratcliffe NA, A reappraisal of insect haemocyte classification by theexamination of blood from 15 different insect orders. Zeitschrift für Zellforschung und Mikroskopische Anatomie, 147(4): Ribeiro C, Brehélin M, Review insect haemocytes: what type of cell is that? Journal of Insect Physiology, 52(5): Şapcaliu A, Rădoi I, Pavel C, Tudor N, Căuia E, Siceanu A, Meiu F, Research regarding haemocyte profile from Apis mellifera carpatica bee haemolymph originated in the south of Romania. Lucrari Stiinifice Medicina Veterinara, 42(2): Tauber OE, Griffiths JT, Multinucleate hemocytes in the roach, Blatta orientalis. Trans. Am. Micros. Soc., 62: Yeager JF, The blood picture of the southern armyworm(prodenia eridania). J. Agric. Res., 71: 1 40., 1982a ()., 19(3): [Fu YL, 1982a. Insect blood cells(). Insect Knowledge, 19(3): ],1982b ()., 19(4): [Fu YL, 1982b. Insect blood cells(). Insect Knowledge, 19(4): ],,,,, , 48(4): [Jia LP, Cui ZF,

8 2 : 547 Wang Q, Wu H, Zhang XM, Morphological study on hemocytes of locusta migratoria manilensis. Chinese Journal of Applied Entomology, 48(4): ], [Jia LP, Comparative study on the morphology of hemocytes of grasshoppers in oedipodidae. Master Degree Thesis. Taiyuan: Shanxi University.],, , 13(4): [Liu CL, Fu YL, Type identification and pathological changes of blood cells of armyworm. Acta Entomologica Sinica, 13(4): ],,, , 19(6): [Wen XL, Lin HX, Pan XJ, Preliminary observation of Periplaneta Australasiae blood cell morphology. Journal of Guangzhou Normal University, 19(6): ],,, , 26(4): [Mo F, Bao HE, Luo FT, Analysis of hemocytes in the larvae of culex pipien quinquefasciatus. Journal of Guiyang Medical College, 26(4): ],,, , 44(2): [Wang SG, Zhou ZJ, Zheng ZM, The morphology and classification of hemocytes in a locust catantops pinguis. Chinese Bulletin of Entomology, 44(2): ],,,,, , 48(4): [Wang Q, Cui ZF, Wang YL, Shen XY, Zhang XM, Comparison among three staining methods to hemocytes of Oxya chinensis. Chinese Journal of Applied Entomology, 48(4): ], : [Xu ZF, General entomology. Beijing: Science Press ]

Microsoft Word 曹伏君_new_.doc

Microsoft Word 曹伏君_new_.doc 45 2 Vol.45, No.2 2014 3 OCEANOLOGIA ET LIMNOLOGIA SINICA Mar., 2014 (Lethrinus erythropterus) * ( 524025) Wright (Lethrinus erythropterus),,,, ; ; ; Q954.56 doi: 10.11693/hyhz20140100011 (Lethrinus erythropterus)