Microsoft Word - 7--白国新.doc

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续斌邱
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1 土壤 (Soils), 2018, 50(2): DOI: /j.cnki.tr 病死动物源氨基酸研制的生物有机肥促生效果研究 ,2 1* ( ) Bacillus amyloliquefaciens SQR9 20% CFU/g 3 20% CFU/g SQR9 SQR9 SQR9 SQR9 SQR9 SQR9 20% SQR9 S144.2 A [1] [2] [3] [4] [5-6] [7-8] 2013 [9] (PGPR) [10] [11] [12] SQR9 SQR9 [14] SQR9 [13] (2017YFD ) (KYCYL201502) (2016YFD YFE ) ( ) PPZY2015A061 ( ) * (lirong@njau.edu.cn) (1990 ) @qq.com

2 g/kg 461g/kg mg/kg P 2 O mg/kg K 2 O mg/kg N g/l P 2 O g/l K 2 O 1.88g/L ph 1.86 Bacillus amyloliquefaciens SQR9 (SQR9) 1 ph g/kg mg/kg mg/kg mg/kg 1.2 (0% 5% 10% 15% 20% 25% 30%) ( 10% ) 40% ~ 50% [15] 500 g( ) 7 d 24 h ph kg (AA+9) 2(AA) 1 3(CF+9) 2 4(OF+9) 3 5(OF) 250 g 20 2% 1.4 SPAD 1.5 SQR9 SQR9 5 cm 10 cm 0 ~ 10 cm 6 5 g 45 ml 30 min 80 30min SQR h SQR9 [15] 10 g 5 g NaCl10 g 2.5% 1000 ml 1% 2 ml/l 1% 4 ml/l CFU/g 1.6 Excel 2010 SPSS 17.0 (least signifycant difference, LSD) (P<0.05) SQR9 <20% SQR9 20% SQR CFU/g SQR9 ( 1) %~ 30% SPAD

3 ( (P<0.05) ) 图 1 不同含量酸解氨基酸水解液对固态发酵过程中功能菌 SQR9 数量的影响 Fig.1 Effects of different concentrations of compound liquid amino acids on numbers of SQR9 after solid state fermentation 20% 0( ) 5% 25% 30% 93.79% 14.79% 22.09% % 10% 15% 0 5% 10% 25% 30% 16.19% 11.09% 5.69% 11.39% 28.29% SPAD 35.19% 0 25% 30% 42.69% 37.29% % 0 25% 30% 26.69% 28.09% 8.69% 20% 2 20% SQR CFU/g 表 1 不同施肥处理黄瓜的株高茎粗叶绿素和鲜干重 Table 1 Effects of different fertilization treatments on biomass of cucumbers (%) (cm) (mm) SPAD (g) (g) ± 2.93 c 8.03 ± 0.21 c ± 5.02 b ± 3.00 b 5.54 ± 0.47 b ± 4.58 b 8.39 ± 0.52 bc ± 4.93 ab ± 7.92 a 6.69 ± 0.49 a ± 4.58 a 8.83 ± 0.32 b ± 2.18 ab ± 2.14 a 6.65 ± 0.21 a ± 1.50 a 9.03 ± 0.25 ab ± 1.87 ab ± 0.63 a 6.88 ± 0.15 a ± 5.46 a 9.33 ± 0.38 a ± 1.41 a ± 5.44 a 7.02 ± 0.47 a ± 1.65 b 8.36 ± 0.40 bc ± 1.32 a ± 6.29 b 5.48 ± 0.74 b ± 3.79 c 7.27 ± 0.64 d ± 9.68 ab ± c 3.75 ± 0.83 c : (P<0.05) 图 2 施用含不同浓度酸解氨基酸研制的生物有机肥黄瓜盆栽根围土中功能菌 SQR9 的数量 Fig.2 Strain SQR9 numbers in cucumber rhizosphere soils under different treatments amended by bio-organic fertilizer produced by adding different amounts of compound liquid amino acids (AA+9) (OF) (OF+9) (AA) 55.39% 32.69% 25.29% (CF+9) CF+9 AA+9 SPAD AA+9 OF OF+9 AA 6.01% 5.41% 4.02% CF % AA+9 OF OF % 3.21% AA CF % 2.02% 3 SQR9 AA+9 SQR CFU/g SQR9 SQR9

4 2 273 图 3 不同处理黄瓜盆栽根围土土中功能菌 SQR9 的含量 Fig.3 Strain SQR9 numbers in cucumber rhizosphere soils under different treatments (AA+9) OF OF+9 AA CF % 31.69% 25.01% 9.89% AA+9 OF OF+9 AA CF % 15.59% 8.49% AA 7.19% SPAD AA+9 OF OF+9 AA+9 OF OF+9 AA CF % 56.02% 34.01% 23.02% 65.01% 38.02% 16.01% 14.01% 4 SQR9 AA+9 SQR CFU/g OF CF SQR9 SQR9 Table 2 表 2 不同施肥处理黄瓜的株高茎粗叶绿素和鲜干重 Effects of different fertilization treatments on plant heights and diameters, chlorophyll contents and biomass of cucumbers (cm) (mm) SPAD (g) (g) OF 9.67 ± 0.58 c 1.99 ± 0.05 c ± 4.93 ab 5.52 ± 0.05 c 0.53 ± 0.03 c OF ± 0.58 b 2.13 ± 0.07 b ± 2.18 ab 5.68 ± 0.07 b 0.54 ± 0.02 bc AA ± 1.00 b 2.14 ± 0.06 b ± 1.87 ab 5.70 ± 0.04 b 0.55 ± 0.02 bc AA ± 0.15 a 2.44 ± 0.08 a ± 1.31 a 5.86 ± 0.11 a 0.58 ± 0.02 b CF ± 0.58 a 2.23 ± 0.02 b ± 1.22 a 5.74 ± 0.05 a 0.57 ± 0.01 b 图 4 不同处理辣椒盆栽根围土中功能菌 SQR9 的含量 Fig.4 Strain SQR9 numbers in pepper rhizosphere soils under different treatments (AA+9) OF OF+9 AA CF % 19.29% 18.09% 12.19% 29.59% 23.39% 19.69% 17.79% SPAD AA+9 OF OF+9 AA AA+9 OF OF+9 AA CF % % 56.01% 27.01% % 40.02% 40.01% 24.02% 5 AA+9 SQR CFU/g OF CF SQR9 3 SQR9

5 表 3 不同施肥处理对辣椒的株高茎粗叶绿素和鲜干重影响 Table 3 Effects of different fertilization treatments on plant heights, stem diameters, chlorophyll contents and biomass of peppers (cm) (mm) SPAD (g) (g) OF ± 0.90 f 2.62 ± 0.02 c ± 0.38 b 7.82 ± 0.18 c 0.84 ± 0.05 b OF ± 0.26 d 3.19 ± 0.10 b ± 0.90 b 9.05 ± 0.24 c 1.25 ± 0.22 a AA ± 0.58 c 3.45 ± 0.92 ab ± 0.25 a ± 0.40 b 1.27 ± 0.05 a AA ± 0.58 a 3.71 ± 0.21 a ± 1.16 a ± 0.69 a 1.47 ± 0.06 a CF ± 0.57 b 3.41 ± b ± 1.02 a ± 0.70 b 1.29 ± 0.06 a 图 5 不同处理玉米盆栽根围土中功能菌 SQR9 的含量 Fig.5 Strain SQR9 numbers in maize rhizosphere soils under different treatments [2,14,17] ph ph 20% [2] SQR9 20% [19] 20% SQR9 20% 表 4 不同施肥处理对玉米的株高茎粗叶绿素和鲜干重影响 Table 4 Effects of different fertilization treatments on plant heights, stem diameters, chlorophyll contents and biomass of maize (cm) (mm) SPAD (g) (g) OF ± 0.19 d 4.96 ± 0.15 c ± 0.15 d 6.56 ± 0.17 d 0.76 ± 0.02 c OF ± 0.52 c 5.21 ± 0.19 bc ± 0.16 c 9.37 ± c 0.97 ± 0.03 b AA ± 0.76 c 5.37 ± 0.28 b ± 0.95 b 9.41 ± 0.12 c 0.99 ± 0.02 b AA ± 0.51 a 6.43 ± 0.09 a ± 0.59 a ± 1.96 a 1.58 ± 0.12 a CF ± 0.50 b 5.46 ± 0.17 b ± 0.10 ab ± 1.21 b 1.11 ± 0.19 b OF OF+9 SQR9 SQR9 [16,18] AA OF AA+9 AA OF+9 SQR9 SQR9 AA+9 CF+9 PGPR IAA ACC [20] [21] [22]

6 ) SQR9 20% 2) [1],,. [J]., 2007(6): 1 5 [2],,,. [J]., 2014, 20(5): [3]. [J]., 2013(10): 45 [4]. [J]., 2015(11): 75 [5],,,. [J]., 2013, 45(2): [6],,,. [J]., 2016, 53(2): [7] Qiu M H, Zhang R F, Xue C, et al. Application of bioorganic fertilizer can control Fusarium wilt of cucumber plants by regulating microbial community of rhizosphere soil[j]. Biology and Fertility of Soils, 2012, 48: [8],,,. [J]., 2012, 45(2): [9],,,. [J]., 2016, 53(1): [10] Liu K, Garrett C, Fadamiro H, et al. Antagonism of black rot in cabbage by mixtures of plant growth-promoting rhizobacteria (PGPR)[J]. BioControl, 2016, 61: [11] Habibi S, Djedidi S, Prongjunthuek K, et al. Erratum to: physiological and genetic characterization of rice nitrogen fixer PGPR isolated from rhizosphere soils of different crop[j]. Plant and Soil, 2014, 379(1/2): [12] Parray J A, Jan S, Kamili A N, et al. Current perspectives on plant growth-promoting rhizobacteria[j]. Journal of Plant Growth Regulation, 2016, 35: [13] Shao J, Li S, Zhang N, et al. Analysis and cloning of the synthetic pathway of the phytohormone indole-3-acetic acid in the plant-beneficial Bacillus amyloliquefaciens SQR9[J]. Microbial Cell Factories, 2015, 14: 130 [14] Cao Y, Zhang ZZ, Ling N, et al. Bacillus subtilis SQR9 can control Fusarium wilt in cucumber by colonizing plant roots[j]. Biology and Fertility of Soils, 2011, 47: [15],,,. [J]., 2016, 48(4): [16] Zhang M, Li R, Cao L L, et al. Algal sludge from Taihu Lake can be utilized to create novel PGPR containing bioorganic fertilizers[j]. Journal of Environmental Management, 2014, 132: [17],,,. [J]., 2014, 37(2): [18],. [J]., 1984(5): [19],,,. [J]., 2015, 38(6): [20] Shao J H, Xu Z H, Zhang N, et al. Contribution of indole-3-acetic acid in the plant growth promotion by the rhizosphere strain Bacillus amyloliquefaciens SQR9[J]. Biology and Fertility Soils, 2015, 51: [21]. Bacillus subtilis SQR9 [D]. :, 2011 [22] Näsholm T, Ekblad A, Nordin A, et al. Boreal forest plants take up organic nitrogen[j]. Nature, 1998, 392:

7 Plant Growth Promotion Effect of Novel Bio-organic Fertilizers Produced by Solid-state Fermentation with Amino Acids Hydrolyzed from Animal Carcasses as Additive BAI Guoxin 1, LIU Shanshan 1, ZHENG Yu 1, SHAO Cheng 1, WANG Dongsheng 1,2, LI Rong 1*, SHEN Qirong 1 (1 Jiangsu Key Laboratory of Solid Organic Waste Utilization, National Engineering Research Center for Organic-based Fertilizers, Key Laboratory of Plant Nutrition and Fertilization in Low-Middle Reaches of the Yangtze River, Ministry of Agriculture, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural University, Nanjing , China; 2 Nanjing Institute of Vegetable Science, Nanjing , China) Abstract: Animal carcasses hydrolyzed by sulfuric acid solution to create compound liquid amino acids is an effectively strategy in environment protection. However, how to efficiently utilize these liquid amino acids does cause public concern. In this study, compound liquid amino acids from animal carcasses were utilized as additives into matured composts to create novel bio-organic fertilizer (BIO) containing plant growth-promoting rhizobacteria (PGPR)Bacillus amyloliquefaciens SQR9, after that the effect on plant growth promotion of novel bio-organic fertilizers were investigated. Results showed that the optimal amendment concentration of liquid amino acids was 20%, which resulted in a maximum biomass of functional strains with the number of CFU/g (dry weight, the same follow) that was 3 times of the control without adding liquid amino acids. Pot experiment results showed that the effect on cucumber plant growth promotion of the novel BIO were superior to the other treatments amended with different concentrations of liquid amino acids and the control. The number of strain SQR9 in rhizosphere soil reached CFU/g soil. Results from pot experiments of different crops showed the novel produced BIO resulted in greater plant growth compared to the treatment amended with BIO produced by adding chemical fertilizer as additive, the treatment amended with BIO produced by adding none additive, the treatment amended with organic fertilizer and the treatment amended with organic fertilizer amended with liquid amino acids. Meanwhile, the treatments amended with the fertilizers with strain SQR9 showed better plant growth than the others, indicating that strainsqr9 exhibited plant growth promotion effect. Moreover, compared to the treatment amended with BIO produced by adding chemical fertilizer as additive, the treatment amended with novel BIO is more beneficial for the function enhancement of the strain SQR9. In the rhizosphere of different crops, significant higher number of functional microbes were observed in the treatment amended with novel BIO. To sum up, adding 20% of liquid amino acids effectively enhanced the growth of functional microbes in the organic fertilizer, and the produced novel BIO showed outstanding plant growth promotion effect. Key words: Bacillus amyloliquefaciens SQR9; Compound liquid amino acids; Animal carcasses; Bio-organic fertilizer; Plant growth promotion effect

Microsoft Word - 17--刘秀秀_new_.doc

Microsoft Word - 17--刘秀秀_new_.doc 土壤 (Soils), 2014, 46(5): 875 880 1 缺钾对油菜主序产量性状的影响及施钾效果 * ( 430070) (+K) ( K) 1 321 kg/hm 2 111% 50.1% 9.2% 33.6% 21.6% 10.3% 69.6% 41.7% 20.1% 124.4% S143.3 57.2% [1] [2] [3] [4 6] 2005