: Cr( ) 1041, 1.1 硫酸盐还原菌的分类及生物学特性 Bergey s, SRB, 8 [1] : Desulfovibrio Desulfomonas Desulfobulbus Desulfotomaculum Desulfococcus Desulfobacter Desulfo

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1 微生物学通报 JUL 20, 2009, 36(7): 1040~1045 Microbiology 专论与综述 2009 by Institute of Microbiology, CAS 硫酸盐还原菌及其还原解毒 Cr(Ⅵ) 的研究进展 * 徐卫华刘云国曾光明李欣彭庆庆 ( ) 摘要 : 硫酸盐还原菌是一类分布广泛, 能进行硫酸盐异化还原反应的严格厌氧菌利用硫酸盐还原菌可去除环境中的许多污染物, 因而该类细菌在环境污染治理中具有广阔的应用前景本文介绍了硫酸盐还原菌的生物学特性和代谢特征及其在环境污染治理中的应用, 并对硫酸盐还原菌还原解毒 Cr(Ⅵ) 及应用于含 Cr(Ⅵ) 废水处理的研究进展作了综述, 分析了其未来的研究方向关键词 : 硫酸盐还原菌, Cr(Ⅵ), 还原 Sulfate-reducing Bacteria and Research Progress of Cr( Ⅵ ) Reduction by Sulfate-reducing Bacteria XU Wei-Hua * LIU Yun-Guo ZENG Guang-Ming LI Xin PENG Qing-Qing (College of Environmental Science and Engineering, Hunan University, Changsha, Hunan , China) Abstract: Sulfate-reducing bacteria (SRB) are widespread in the environment. SRB are obligate anaerobes and capable of dissimilatory reduction of sulfate. SRB have application prospects in the control of environmental pollution due to that many pollutants can be removed by SRB. The biological characteristics and metabolic mechanisms of SRB are introduced, and the application of SRB in the treatment of environmental pollution is described in this paper. The research progress of Cr( Ⅵ ) reduction and Cr( Ⅵ ) removal from wastewater by SRB is reviewed, and future direction of research on the control of Cr( Ⅵ ) pollution by SRB is also analysed. Keywords: Sulfate-reducing bacteria, Cr( Ⅵ), Reduction (Sulfate-reducing bacteria, SRB) SRB, SRB,, SRB ( ), Cr( ), Cr( ),, Cr( ), SRB Cr( ) 1 硫酸盐还原菌的特性 SRB SRB,, * 通讯作者 :Tel: ; : amyweihuaxu@yahoo.com.cn 收稿日期 : ; 接受日期 :

2 : Cr( ) 1041, 1.1 硫酸盐还原菌的分类及生物学特性 Bergey s, SRB, 8 [1] : Desulfovibrio Desulfomonas Desulfobulbus Desulfotomaculum Desulfococcus Desulfobacter Desulfosarcina Desulfonema SRB 40,, [2], : H 2, ; ( ), SRB : CO 2 H 2 O;, SRB SRB, 100, SRB [2,3] ph SRB, SRB ph SRB, SRB, SRB H 2 S, H 2 S, SRB,, SRB, SRB 1.2 硫酸盐还原菌的代谢机理 SRB 3 [3] : ( 1) 1, SRB ( ), ATP ; 2, SRB ( C 3 ), ATP;,, S 2,, SRB,, SRB 图 1 硫酸盐还原菌的分解代谢过程 Fig. 1 Metabolic process in sulfate-reducing bacteria 1.3 硫酸盐还原菌在环境污染治理中的应用 SRB SRB, SRB SO 2 4 H 2 S H 2 S, [4] [5] SRB Fe 3+ Mn 2+ Ni 2+ Zn 2+ Cu 2+, SO 2 4 H 2 S,, Fe 3+ Mn 2+ Ni 2+ Zn 2+ Cu %, 93.1%, 98.2%, 89.9%, 95.6% Jong Parry [6] SRB SO 2 4, Cu Zn Ni 97.5% SRB SO 2 4, SRB [7], SRB SRB, SO 2 4 H 2 S H 2 S, SRB, [8,9] 70, Wei [10] SRB, SRB, SRB Kosińska Miśkiewicz [11], SRB, COD

3 1042 微生物学通报 2009, Vol.36, No.7 98% 88% Chockalingam Subramanian [12] (Eucalyptus tereticornis), Desulfoto- maculum nigrificans, ph % 84% [13], SRB, SO %, SRB Lee Sublette [14], ph 7.0, 25 C, SO 2 1 s~2 s, SO 2 H 2 S 2 硫酸盐还原菌还原解毒 Cr(Ⅵ) 的研究 SRB 1895,,, SRB Cr( ), 20, SRB ( ), Cr( ) SRB (Desulfovibrio sp.) [15 17] SRB Cr( ) 3 : 1) Cr( ) SRB, SRB SRB Cr( ) ; 2) SRB Cr( ), SRB, Cr( ) ; 3) SRB Cr( ), 2.1 硫酸盐还原菌还原 Cr( Ⅵ ) 的影响因素 ph SRB Cr( ),, SRB ph 30 C~37 C Cr( ) [15] Cr( ) SRB, 30 C ph 7.0, mol/l Cr( ), Cr( ) 99.8% Humphries [17], Desulfovibrio vulgaris Cr( ) ph C~37 C SRB Cr( ) SRB Cr( ),, Cr( ),, Desulfovibrio vulgaris Cr( ),,, Cr( ) [17] SRB Cr( ) [18] SRB Cr( ),, Sr 2+ Zn 2+ UO 2 2+, Ca 2+ Ag +, Cl SO 4 2 CO 3 2, SiF 6 2 EDTA Cr( ) SRB Cr( ) Cr( ), Cr( ), SRB, Cr( ) SRB Cr( ),, Cr( ) [19], SRB Cr( ),, 2.2 硫酸盐还原菌还原 Cr(Ⅵ) 的机理 SRB Cr( ) : Cr( ) SRB SO 4 2 H 2 S Cr( ) ; Cr( ) [16] Cr( ), Cr( ), Cr( ), ( S 2 ) Cr( ) Cr( ) Fude [20] SRB Cr( ) Cr( ) SRB SO 4 2 H 2 S Cr( ) SRB Cr( ) Smith [21] SRB, H 2 S,, Cr( ) SRB Cr( ),, SRB Cr( ) :

4 : Cr( ) 1043 c Cr( ), SRB, Cr( ), Cr( ) Cr( ) 1994, Lovely [22] Desulfovibrio vulgaris c 3, 2001, Michel [23] SRB Cr( ) c, Desulfomi- crobium norvegicum c 3 Desulfovibrio vulgaris [Fe], Desulfovibrio vulgaris c 553 Cr( ) ( 1), c 553 ( +50mV), ( ) 2003, Chardin [24] Desulfovibrio vulgaris Hildenborough Desulfomicrobium norvegicum Desulfovibrio fructosovorans 3 : [Fe] [NiFeSe] [NiFe], [Fe], Cr( ) 表 1 硫酸盐还原菌中的 Cr(Ⅵ) 还原酶 Table 1 Cr( ) reductase purified from sulfate-reducing bacteria Sulfate-reducing bacteria Enzymes Cr( ) Rate of Cr( ) reduction [Cr( )/(min mg)] Desulfovibrio vulgaris c 3 391±18 Desulfomicrobium norvegicum c 3 739±56 Desulfuromonas acetoxidans c 7 557±25 Desulfovibrio vulgaris c 553 Desulfovibrio vulgaris [Fe] 7679±4 Desulfomicrobium norvegicum [Ni-Fe-Se] 161±3 References [23] 2.3 硫酸盐还原菌处理含 Cr(Ⅵ) 废水的研究 SRB Cr( ), SRB Cr( ) [25] SRB,,, 0.015% 0.1%, Cr( ) 100%, Cr(OH) 3, Çetin [26] SRB Cr( ), Cr( ) 22.7 mg/l~74.9 mg/l, 2 d~6 d, 99% Cr( ) SRB Cr( ), SRB, SRB Cr( ) [27], SRB,, Cr( ) 99.68%, Cr 0.45 mg/l,,,,, SRB Cr( ) Battaglia-Brunet [28] Desulfomicrobium norvegicum Cr( ),,, Cr( ) Chang [29] Cr( ) SO 2 4, Cr( ) SO 2 4, Cr( ) SO 2 4 ( ), Cr( ) SRB SO 结论与展望,,, SRB, Cr( ) Cr( ), Cr( )

5 1044 微生物学通报 2009, Vol.36, No.7, SRB Cr( ), : 1), SRB Cr( ) Desulfovibrio vulgaris Desulfomicrobium norvegicum SRB, SRB Cr( ), SRB Cr( ) 2) SRB, SRB Cr( ), Cr( ) 3), SRB Cr( ), SRB Cr( ),, SRB Cr( ) 4) Cr( ),, SRB Cr( ), SRB Cr( ) 参考文献 [1],.., 2000, 1(5): [2],,,.., 2006, 26(3): [3],,.., 1997, 19(5): [4] Cabrera G, Pérez R, Gómez JM, et al. Toxic effects of dissolved heavy metals on Desulfovibrio vulgaris and Desulfovibrio sp. strains. Journal of Hazardous Materials, 2006, A135: [5],. EGSB., 2008, 37(2): [6] Jong T, Parry DL. Removal of sulfate and heavy metals by sulfate reducing bacteria in short-term bench scale upflow anaerobic packed bed reactor runs. Water Research, 2003, 14(37): [7],,. Cu 2+., 2005, 31(9): [8] Pruden A, Messner N, Pereyra L, et al. The effect of inoculum on the performance of sulfate-reducing columns treating heavy metal contaminated water. Water Research, 2007, 41: [9] Neculita CM, Zagury GJ, Bussière B. Effectiveness of sulfate-reducing passive bioreactors for treating highly contaminated acid mine drainage: Ⅱ. Metal removal mechanisms and potential mobility. Applied Geochemistry, 2008, 23: [10] Wei L, Ma F, Wang Q, et al. Investigation of the reduction performance of sulfate-reducing bacteria enhanced by nano-meter/submicron tourmaline. Journal of Biotechnology, 2008, 136S: S142. [11] Kosińska K, Miśkiewicz T. Performance of an anaerobic bioreactor with biomass recycling, continuously removing COD and sulphate from industrial wastes. Bioresource Technology, 2009, 100: [12] Chockalingam E, Subramanian S. Utility of Eucalyptus tereticornis (Smith) bark and Desulfotomaculum nigrificans for the remediation of acid mine drainage. Bioresource Technology, 2009, 100: [13],.., 2000, 16(2): [14] Lee KH, Sublette KL. Simultaneous combined microbial removal of sulfur dioxide and nitric oxide from a gas stream. Applied Biochemistry and Biotechnology, 1991, 28/29: 623. [15],,. ( )., 1994, 14(6): 1 4. [16],,,. Cr( ). ( ), 2005, 3(1): [17] Humphries AC, Macaskie LE. Reduction of Cr( ) by Desulfovibrio vulgaris and Microbacterium sp.. Biotechnology Letters, 2002, 24: [18],,,. ( )., 2005, 25(1): 1 4. [19],,,. (SRB)., 1995, 17(4): [20] Fude L, Harris B, Urrutia MM, et al. Reduction of Cr( ) by a consortium of sulfate-reducing bacteria (SRBШ). Applied and Environmental Microbiology, 1994, 60(5): [21] Smith WL, Gadd GM. Reduction and precipitation of chromate by mixed culture sulphate-reducing bacterial biofilms. Journal of Applied Microbiology, 2000, 88: [22] Lovley DR, Phillips EJP. Reduction of chromate by Desulfovibrio vulgaris and its c 3 cytochrome. Appl Environ Microbiol, 1994, 60: [23] Michel C, Brugna M, Aubert C, et al. Enzymatic reduction of chromate: comparative studies using sulfate-reducing bacteria. Appl Microbiol Biotechnol, 2001, 55: [24] Chardin B, Giudici-Orticoni MT, Luca GD, et al. Hydrogenases in sulfate-reducing bacteria function as chromium

6 : Cr( ) 1045 reductase. Appl Microbiol Biotechnol, 2003, 63: [25],,,.., 1997, 7(1): [26] Çetin D, Dönmez S, Dönmez G. The treatment of textile wastewater including chromium( ) and reactive dye by sulfate-reducing bacterial enrichment. Journal of Environmental Management, 2008, 88: [27],,,.. ( ), 2005, 36(6): [28] Battaglia-Brunet F, Foucher S, Morin D, et al. Chromate(CrO 2 4 ) reduction in groundwaters by using reductive bacteria in fixed-bed bioreactors. Water, Air, and Soil Pollution: Focus, 2004, 4: [29] Chang IS, Kim BH. Effect of sulfate reduction activity on biological treatment of hexavalent chromium [Cr( )] contaminated electroplating wastewater under sulfate-rich condition. Chemosphere, 2007, 68: ( p.1030) 征稿简则 : 1), ; 2),,,, ; 3),, ; 4),, 5),, DNA, ATP ; 6), 3.4.2,, DNA RNA, EMBL ( ) GenBank ( ) DDBJ( ), (Accession No.) , 4.2.2, ( ), 4.2.3,, 4.2.4,, ,, 1 ,,,,,, 4.3.2,,,,, 5, 6 : 1 3 (100101) Tel: tongbao@im.ac.cn :

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