HEREDITAS (Beijing) 2011 7, 33(7): 707 712 ISSN 0253-9772 www.chinagene.cn 综述 DOI: 10.3724/SP.J.1005.2011.00707 鲟形目鱼类内分泌激素的分子生物学研究 曹宏, 周荣家, 430072 鲟鱼是处于软骨鱼类向硬骨鱼类过渡的一类古老物种, 被认为是研究脊椎动物起源和演化的重要物种之一 目前, 绝大多数种类的鲟鱼都处于濒危状态, 因此, 采用各种手段来保证其物种的繁衍刻不容缓 但是到目前为止, 关于鲟鱼的生长和生殖调控的分子机制相关信息, 依然十分有限 文章就近年来对鲟鱼的各类内分泌激素基因的研究进展作一概述, 为进一步深入研究鲟鱼的生长和生殖调控的分子机制提供有益的参考资料 鲟鱼 ; 内分泌激素 ; 生长 ; 生殖 Molecular biological study on hormones in Acipenseriformes CAO Hong, ZHOU Rong-Jia College of Life Sciences, Wuhan University, Wuhan 430072, China Abstract: Sturgeons belong to the Acipenseriformes, which provide an ideal model for evolutionary studies due to their unique characters. At present, many species of Acipenseriformes are very rare and near extinct. It is urgent to protect these species. However, data on molecular mechanisms of their growth and reproduction regulation are still limited. Hormones are important factors involved in these processes. In this paper, we summarized recent research progresses in the hormones in sturgeon, which will provide valuable information for further studies on molecular mechanisms of growth, sexual development, and reproduction regulation in Acipenseriformes. Keywords: Acipenseriformes; hormone; growth; reproduction (Acipenseriformes),, ( 4.5~3.5 ),, (Chondrostei),,,,,, (Acipenser sinensis),,,,,,,,,, 收稿日期 : 2011 01 20; 修回日期 : 2011 04 15 基金项目 : ( 30900139) (973 ) ( 2010CB126306) 作者简介 :,, Tel: 027-87889292; E-mail: regancao@ihb.ac.cn 通讯作者 :,,, E-mail: rjzhou@whu.edu.cn 网络出版时间 : 2011-5-30 11:38:47 URL: http://www.cnki.net/kcms/detail/11.1913.r.20110530.1138.007.html
708 HEREDITAS (Beijing) 2011 33,, ; [1] 20 70,,,,,, [2], [1], [3], [4], [5],,, [6~8], (Acipenser gueldenstaedtii) (Acipenser baeri), (Acipenser transmontanus),,,,, 1 (Growth hormone, GH), (Insulin-like growth factor, IGF) GH,,,, [9] GH GH (Growth hormone receptor, GHR), GHR GHR,,, GH 80, Farmer [10],,, Yasuda [11] 2 GH, 3,, GH, GH GH, 63%~76%, GH 42%~ 63% Din [12] GH cdna, 980 bp, 642 bp, 214 1 5, GH mrna -I (IGF-I), cdna 445 bp, 396 bp, 132, 44, IGF-I,,, mrna GH, 1 5, IGF-I mrna Cao [6] GH, GH cdna 945 bp, 642 bp, 214, 1, GH mrna Western blotting, 1, GH, 2, GH, 1~2, 3, GH,, GH Liao [7] GHR (Chinese sturgeon GHR, csghr) csghr cdna 1 833 611 csghr 19 5 5 N- (FGDFS) 219 24 ( )
7 : 709 Box 1 Box 2 9 349 (CHO) csghr csghr, csghr 2.1(Spi 1.2) (seabream ) (seabream GH, sbgh), sbgh, CHO csghr, GH GHR csghr (Growth hormone binding protein, GHBP) csghbp, csghbp, csghr Asp Glu csghr, Asp Ala csghr, csghr, csghbp csghr GHR, Tarpey [13], GHR GH, GHR GHR GHR, GH, GHR, Fukamachi [14] (Huso dauricus) GHR, cdna 2 448 bp, 1 833 bp, 571, GHR (Protopterus dolloi) GHR, GHR, (Somatolactin receptor, SLR) SLR GHR,,, SLR, GHR SLR, GHR SLR 2 (Gonadotropin, GTH),, (Follicle-stimulating hormone, FSH), (Luteinizing hormone, LH) Moberg [15] FSHβ LHβ, GTH I GTH II,,, FSHβ LHβ,, LHβ FSHβ Quérat [16] FSHβ LHβ, FSHβ cdna 1 051 bp, 128, 20 ; LHβ cdna 570 bp, 137, 22, FSHβ, FSHβ FSHβ ; LHβ LHβ, 4 ; 2.5, FSHβ LHβ Hurvitz [17] GTHα FSHβ LHβ, FSHβ mrna, LHβ mrna ;, LHβ mrna FSHβ mrna, 1, FSHβ mrna, 2, FSHβ mrna, 4 [18] (Acipenser schrenkii) 2 β, FSHβ, 99.1%;, 34.5% LHβ 98.3%;
710 HEREDITAS (Beijing) 2011 33 60%,, 42.8% FSHβ LHβ Cao [8] GTHα FSHβ LHβ,, Western blotting 3 3, GTHα (Pars intermedia, PI) (Rostral pars distalis, RPD) (Proximal pars distalis, PPD); FSHβ PPD ; LHβ PI PPD RPD, FSHβ LHβ 1~5, Western blotting FSHβ, 4 5 4 PPD RPD,, 3 (Somatolactin, SL) (Growth hormone, GH) (Prolactin, PRL), [19] SL Amemiya [20] SL cdna 881 bp, 696 bp, 232, 24 208, SL (Melanocyte stimulating hormone, MSH) SL, cdna 1 143 bp, 696 bp, 232 2 3 4 RT-PCR, SL, 3 ( ) 4, (Somatostatin, SS) 20 70 14,, (SSR),, 1995, Nishii [21], GH Kim [22] (Scaphirhynchus albus), SS, Trabucchi [23] 2 SS, SS-14 [Pro(2)] SS-14,, 2 SS cdna, 116, 3 SS-14, PSS1 111, 3 [Pro(2)] SS-14, PSS2 PSS2 PSS2 67%, PSS1 ; PSS2 PSS2 mrna Adrio [24], SS, SS,, SS, SS, Li [25] SS, 2, AsSS1 AsSS2, 2, AsSS1, AsSS2,
7 : 711, AsSS2 mrna, 5,, AsSS2 AsSS1 5 (Proopiomelanocortin, POMC) γ- (γ-melanocyte stimulating hormone, γ-msh) ACTH ( α-msh (α-, α-melanocyte stimulating hormone) CLIP (, Corticotropin-like intermediate lobe peptide)) γ- (γ-lipotropic hormone, γ-lph) β- (β-melanocyte stimulating hormone, β-msh) β- (β-endorphin) Alrubaian [26] POMC cdna, 2 POMC, POMC A POMC B 2 POMC 45, 26 Danielson [27] (Polyodon spathula) 2 POMC, γ-msh, 6 Quérat [16] (Thyroid-stimulating hormone, TSH), cdna 1 010 bp, 143, 20 TSH, TSH TSH FSHβ, : FSHβ, TSH, LHβ 7,,,, ;,,,, [28, 29],,,,,, 参考文献 (References): [1] 粦,, 玕,.., 1985, 9(2): 99 110. [2],,,,.., 1985, (3): 22 24. [3],,,.., 1985, (4): 38 41. [4],,,,,,,,,,,.., 1985, (1): 1 5. [5],,,,.., 2004, 28(6): 633 639. [6] Cao H, Zhou RJ, Wei QW, Li CJ, Gui JF. Molecular characterization of the growth hormone in Chinese sturgeon including expression during embryogenesis and early larval stages. J Appl Ichthyol, 2011, 27(2): 501 504. [7] Liao ZY, Chen XL, Wu MJ. Molecular cloning and functional analysis of Chinese sturgeon (Acipenser sinensis) growth hormone receptor. Sci China Ser C, 2009, 52(10): 911 921. [8] Cao H, Zhou L, Zhang YZ, Wei QW, Chen XH, Gui JF.
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