1
2 1 1 ELISA 90 PCR 2 HAV SRSV BSE FMD VS 2 1 2 3 4 5 POPs 3
3
1 2 3600 4800 80 90 100 100 300 3 4
5
2002 3 1 3 O157H7 FAO/WHO 60 80 1997 2000 FDA FSIS FSIS 9100 1100 7400 6200 EPA 3.5 20 1 6
2 60% R-BIOPHARM RANDOX R-BIOPHARM ELISA ELISA ELISA ELISA / ELISA ELISA ELISA / DNA PCR cdna 7
1 1998 2000 13.1% 2000 9260 12.12% 5% 3 1 0.3 1 2000 6046 1 4 1990 2000 4279 1 3 9 934 38.3 6 2000 6000 4 WTO 21 40 40% 3 WTO 2 2001 9 20 20 40 1.5 400 500 30 40 8
20 40 20 4000 10 150 2000 15 15% 2.25 163 300 23 391 869 3000 117 150 3000 80 15% 12 15 GC-MS HPLC 9
10 ELISA HPLC RANDOX R-BIOPHARM
11
1999 29% 2000 35% 2001 2000 150 6237 135 1 136 123 50 SN 20 22 15 15 /AOAC 2 1 12
0.01 5 90% 0.1 5 85% 1 5 80% 15 10 15 30% 50% ELISA 2 TU-1800 40 2 13
DEP DDVP 1605 1605 MPP 3911 4049 1 -- 37 µg/ml 0.4 0.8 1.6 35.0 52.0 69.0 µg/ml 0.4 0.6 0.8 1.2 1.6 3.75 26.4 29.4 36.7 52.7 59.6 76.3 µg/ml 0.1 0.15 0.2 0.25 0.4 0.5 13.6 22.9 37.2 50.6 63.9 73.9 µg/ml 0.001 0.002 0.003 0.004 0.006 0.008 14
25.2 50.3 58.8 66.8 73.2 78.9 2 CL_BIII CL_BIII JB T9324-1999 WHO FAO WHO EPA JB T9324 1 1979 56 1996 1485 20 26.5 46% 43% 11% 1996 45.8% 1987 1998 247 225 2000 1 63 15
90% 1500 50-2 - NY438-2001 HPLC- GC-MS HPLC-MS/MS ELISA HPLC GC-MS 1000 - ELISA 1000 1 ELISA ELISA 0.5 ppb 1 ppb 2 3 0.1ng/g 1800 4 R-BIOPHARM RANDOX ELISA 16
5 ELISA 0.1ppb 3600 1 100 200 29 29 1 1 2001 800 2002 4 2 1 2002 10 2 17
aflatoxin AF B1 AFB1 AFB1 ELISA AFB1 89.4% Sigma Latex AFB1 3.33mg ml 30 20 150 200 20 TTX ELISA 10 16% 1/3 1/2 PCR 18
PCR DNA ELISA 2002 3 23 PCR 4 RT-PCR PCR 37 -- PCR RIA EIA FIA TrFIA CIA BIA ELISA Weston Blot RT-PCR 19
4 20
1 2 3 4 21
1 PCR DNA PCR 2 60085 80% 10 48000 15.6 1 25% 15000 64% 38000 20 DTNB 480 BTCI 360 3 22
, 23
1 1 1.5 5 863 15 4 5 50 30 1986 2 2000 200 2 3 WTO 2 1 2 3 4 5 24
WTO 10% 5% 6 7 3 1 2 3 WYO 4 5 6 7 8 9 25