1. 2. ( ) (Lux) 1. 100Lux 2. 200Lux ( ) 1
1. 2. 10% 1. ( )2. 3. ()() 2
2. 3. 10~15 1. 1. -18 C 2. 7 C 3
20g 100mL 12.7g 1mL 1000mL 1. 2. 3. 4. 1. ( ()) 2. (sudan IV) (sudan III) 4
0.1g 100mL 1. 2. 4. 3. () 1. 2. ABS 1% (azure A) 10% 5
ph 1. 2. 5mL 3. 5. 10% 6. 4. 1% ph3 7. ABS 1. 2. ( ) 6
10% 10% 1. (2. ( )10% ) 3. ( ) 4. 5. 6. 7. 10% 8. 9. ( ) ( ) ( ) 1. 10% 2. 7
(titanium sulfate solution)( 20~40%) (vanadium pentoxide)( ) ( ) ( ) ph (ph5.0~8.0) 5% 0.1g (4.5mL 100mL)l00mL 1~2 10% 1. 2. 5% 8
3. 4. 10% * " " (p-rosanilin HCI)( ) (formalin 37% ) ( ) (malachite green)( ) : (1) A - 9
1.a 0.2g 100mL 20mL HC16mL 100mL 2.b 0.2% : 3g 500mL 3.a b (2) B (phl.09lm 50mL ln 70mL 130mL (3) CN/200 (4) D0.01% 1. ( ) 2. 10mL A 1mL B4mL 30~35 10mL 3. 5~l0mL C 1~2 4. 5~l0mL D 1~2 1. 2. ( ()) () 10
a- (a-naphthylamine)( ). (sulfanilic acid)( ) (tartaric acid)( ) Griess-Romijn's a-naphthylamine l sulfanilic acid l0 tartaric acid 89 1. 10g( ) 2. 2mL (2SmL) Griess Romijn's 0.5g (Water bath 60 C) 20 () 11
. (sodium tetraborate decahydrate) (potassium ferrocyanide trihydrate) (zinc acetate) (acetic acid) (sulfanilamide) [N-l(l-naphthyl)ethylenediamide- 2HCl] (sodium nitrite99%) (1) : 50g 1000mL (2) A: 10.6g 100 ml (3) B: 22g 3mL IOOmL (4) A: 0.5g HCl(1 1)100 ml (5) B: 0.12g 100mL (6) : 1.500g ( 100 C 30 ) 1000mL (NO2-)lmg ml (NO2-)50ug 12
1. 5g( ) l00ml 5mL 80 C 80mL 15 AB 2mL 100mL 2. (NO2-50"g/mL) 1356 9mL 100mL 80mL 540nm 3. 4mL 20mL A lml BlmL l0 20mL540nm (C) NO2- (ppm) NO2-(ppm)=Cx100/W C: (ug/ml) W: (g) () (chromotropic acid) (85%) ph : (1)2% 2g 100mL (2)20% 13
1. 10g( 3mL( ) 2. 1mL ) ( 2% 1~2 20mL)20% 1mL 10 1mL () (potassium periodate) (sulfuric acid) (hydrochloric acid) (potassium hydroxide) (hexamine,99.5%) 4-amino-3-hydrazino-5-mercapto -1,2,4-triazole(AHMT) : 14
(1) AHMT :AHMT0.5g0.5N HCl l00ml (2) KIO4 : Kio4 0.75g0.2N KOH 100mL (3) 2N H2SO4 (4) 5N KOH (5) 0.5N HCl (6) 0.2N KOH (7) : 0.3112g 1000mL ml (HCHO)400ug 1. : 10g( lml l0ml 2. : 3. : )5N KOH HCHO 400ug/mL)0.5,1 2N lml 80mL 2,4 6mL H2SO4 3 l0 100mL 80mL 50 C 3 ug/ml 5N KOH lml 550nm AHMT lml 5 KIO4 lml 550 nm (C) (HCHO) (ppm) HCHO(ppm)=Cx100/W c: w: (g) 15
(Auramine) (1% ) (1% ) (30mesh) ( 30 ) 1. 5g 2. 1% 10mL 3. 5cm 5 5 4. 5. 6. 0.5g 1% 5mL 16
7. 1. 2. A B C A 17
B C Metanil Yellow Metanil Yellow 18
( ) ( ) ( ) 10% ( ) 1. 2. 3. 4. Metanil Yellow 10%... 18 24 A. 19
20 1. lml 2. B. 1. lml 2. C. 1. 2. 1mL 3. 37.c 18~24 4. 24 (CFU/g) ( )
1. : (a) 10 100 1000 2. : 10000 (c) (b) lml (spreader) 20 cm2 (d) 1 (e) ( 20~200 )35 C 24~48 ( ) 21
(Staphylococcus aureus) 1. 2. 3. ( 1.5Cm) 4. 5. 30 2 (1) ( ( ) ( ) ( (2) trypticase soy agar,nutrient agar blood agar (uninterpret- able) (3) (1~2mm )2~6 (false po- sitive) 22
(4) (stringy) (milky background) (5) 2~8C (sonicator) 2 3 NaN3 1. 2. 34 c~49 c 23
24 3..4. 100 c 2 ( 200ppm) 5. ( ) 1. 2. 3. 4. 1. 2. 3. 4. ( ) 1. 2. 1. 2. 3. 1. 2. 3. 1. ( ) 2. 3. 1.
2. 3. 1. 2. 3. 4. 5. 6% 1. 2. 3. 4. 5. 6% 1. 2. 3. 5-10mL -1~2-2 - 4. 10mL +50% 2 - - 1. 2. 1. 2. 1. 0.5~2g 2. 100mL 10mL 0.1~2g 3~5 25
3.4. 26
27