. 26 : 153-165 2008 Ann Rept. BFDA Taiwan R.O.C. 26 : 153-165 2008 153 Acanthopanacis Cortex Araliaceae Acanthopanax gracilistylus W. W. Smith Acanthopanax giraldii HarmsPeriplec sepium Bge.Lycium chinense Mill. Periplocin Nested PCR DNA 43 43 920.9%3274.4%2 4.7% (1) (1~14) (5-12) SyringinB1 Eleutheroside B1 (7,9-13) (5-7,9-14) Asclepiadaceae P. sepium (2,3,7-9,11,13) Periplocin Glycoside G K H 1 E Glycoside K H 1 E (7,9,11) (9,11,13) Solanaceae L. chinense (1-6,8-11,13-18) (6,9-11,17) Linolenic acidbetaine (9-11,16-18) (5,6,9)
154 Ann. Rept. BFDA ㈠ 1. Olympus BX51 Evolution /Qlmaging Digital Camera kit 2. AO820 Rotary microtome 3. Forma Scientific 6512 Vaccum Oven 4. Buchi Labortoriums-Technik AG CH-9230 FLAWIL/SCHWEIZ 5. CAMAG Temp 20220 Corning Hot Plate, U.S.A. 6. 7. E. Merck Silica gel 60 F 254 20 x 20 cm 8. TLC Gel Catcher Canon G1 ㈡ 1. Merck KantoLab-Scan Riedel dehaensafranin FlukaFast green WakoCanada Balsam E. Merck Alps Santoku Merck Lab- ScanLab-ScanHPLC 2. FAAFormalin 5 ml 5 ml 70% 90 ml 3. eleutheroside B1 Chroma- Dex ㈢ 1. 2. 16 243 43 3. 2 ㈠ (3) ㈡ (19,20) 5% FAA Paraffin Method safranin fast-green ㈢ (19,20) 30 1 4 5 v/v 50 ㈣ 1. eleutheroside B1 1mg/mL 2. 5 g 20 ml 1 2 ml 5 g 20 ml 1 2 ml 3. 7 2 1 v/v Vanillin/H 2 SO 4 spray reagent 105 2 U.V. 366 nm
155 TLC (21) ㈠( 1.1) 0.2 cm ㈡ ( 1.2) A B C 1.1 AB C A B C 1.2 AB C
156 Ann. Rept. BFDA 2~5 mm ㈢( 1.3) ㈣( 1.4) A B C 1.3 AB C A B C 1.4 AB C
157 ca ㈠( 2.1~2.3) 1. 2. 3. 1~8 4. 4~11 5. kl ca 2.3() ㈡ ( 2.4~2.5) 1. 1 2. 3. sc epl mph kl co p 2.1 2.4 sc ca 2.2 2.5 ()
158 Ann. Rept. BFDA 4. 5. 3~4 kl cr lt mph 6. ㈢( 2.6~2.8) 1. 3~30 2. 3. 1~5 4. ㈣( 2.9~2.10) 1. 2~3 2. 3. 4. 5. 2.6 lt kl obs 2.7 2.9 cr cd 2.8() 2.10()
159 ㈠( 3.1~3.4) 1. 2. 3. 4. 2~10 ㈡( 3.5~3.10) 1. 2. 3. 4. 5. ㈢ ( 3.11~3.12) 1. 2. 3. 4. 5. 2~6 ㈣( 3.13~3.16) 1. 2. 3.1 3.2 3.3 3.4
160 Ann. Rept. BFDA 3.5 3.6 3.7 3.8 3.9 3.10
161 3.11 3.12 3.13 3.14 3.15 3.16
162 Ann. Rept. BFDA 3. 4. 5. 2~8 eleutheroside B1 7 2 1 v/v Vanillin/H 2 SO 4 spray reagent 105 2 U.V. 366 nm R f 0.49eleutheroside B1 R f 0.14 R f 0.27 R f 0.46 R f 0.21 R f 0.27 R f 0.38 R f 0.49eleutheroside B1 R f 0.27 R f 0.46 TLC 2 95 PCR-DNA 2 eleutheroside B1 2 PCR-DNA TLC 4.1~4.2 TLC eleutheroside B1 16 743.7% 1 2 3 4 5 6 7 4.1 1. 2. eleutheroside B1 3. 4. 5. 6. 7. 1 2 3 4 5 6 7 4.2 1. 2. eleutheroside B1 3. 4. 5. 6. 7.
163 7~14 3~4 15~30 2~6 3~8 1. 2. Periplec sepium 2. Lycium chinense 1. 2. 3. Acanthopanax gracilistylus 3. Acanthopanax giraldii 743.7%212.6% 2414.2% 2395.8%3 133.3%266.7% PCR-DNA (21) ca Clustered crystal cd Crystal sand co Collenchyma cr Crystal epl Lower epidermis kl Cork layer lt Latex tube mph Phloem medullary ray obs Obliterated sieve p parenchyma sc Secretory canal
164 Ann. Rept. BFDA TLC 56.3% 43 920.9% 3274.4%24.7% 43 920.9% 3274.4%24.7% Periplocin Glycoside G 1. 1999 -PP.43~44 55~56 2. - 1997(3) pp.194~195 199 212 3. 1998 pp.102~103 185 371 4. 2 0 0 1 pp.73~74 109~110 5. 2002pp.113~114 404~405 6. 1 9 9 6 ( ) pp.360~362 366~369 7. 2000 PP.121~123 574~576 8. 2006 (II) pp.484~493 9. 1 9 9 3 pp.139~141 308~309 626~627 10. 2001 () PP.356~358 775~778 11. 2 0 0 2 pp.527~551 567~574 628~632 12. 1995 ( ) pp.672~756 13. 2005 pp.44 82 181 14. 1 9 9 8 pp.64~66 76~78 15. 2003 PP.69~70 16. 1 9 9 4 PP.182~185 17. 2000 ( ) pp.116~123 18. 1 9 9 9 PP.411~412 19. 2002 (I) pp.1~30 20. 1975 pp.37~47 21. 2006 Nested PCR DNA
165 DOH95-FD-2009 Studies on Adulteration and Misusage of Acanthopanacis Cortex in Market YA-HUI HSU, TSAI-YU WEN, FANG-SU LIU, CHI-FANG LO AND JER-HUEI LIN PHARMACOGNOSY DIVISION ABSTRACT Alternatives and adulterants of raw materials may affect the therapeutic efficacy of Chinese medicinal preparations, and pose harm to health. Therefore, authentic raw materials are the priority issue for the safety and quality of traditional Chinese medicines. The botanical origin of Acanthopanacis Cortex is dried root bark of Acanthopanax gracilistylus. According to literatures, there are a number of alternatives and adulterants, such as Periploca sepium Beg., Acanthopanax giraldii Harms., and Lycium chinense Mill, etc. In order to identify the botanical origins of commercial Acanthopanacis Cortex in Taiwan, forty-three samples were purchased from market and were distinguished from authentic materials by morphology, microscopy, thin layer chromatographic analysis and DNA sequencing. The results showed that 9 out of 43 samples (20.9%) were A. gracilistylus, 32 samples (74.4%) were P. sepium and 2 samples (4.7%) were A. giraldii. According to the results, the adulteration of Acanthopanacis Cortex in market is very serious. key words: Acanthopanax gracilistylus, Periploca sepium, Acanthopanax giraldii, identified