6 2 Vol. 6 No. 2 2015 2 Journal of Food Safety and Quality Feb, 2015 VIDAS 王似锦 *, 高春 ( ( ), 100035) 摘要 : 目的 (VIDAS) 方法 21 BPW BPW, VIDAS, 结果 21, BPW ; BPW 20 VIDAS GB, 95.2% 结论 VIDAS 96 h, VIDAS 48 h VIDAS,, 关键词 : ; ; ; Fast detection of Salmonella in health food by VIDAS method WANG Si-Jin *, GAO Chun (Beijing Institute for Drug Control (Beijing Center for Health Food and Cosmetics Control), Beijing 100035, China) ABSTRACT: Objective The automated immunoassay analyzer (VIDAS) was applied to detect the Salmonella in health food. Methods The 21 lots of health food were divided into BPW group and BPW positive control group. The Salmonella in 21 lots of health foods were detected by both VIDAS method and GB method. The results were compared. Results All the results were negative in BPW group. Results of both VIDAS method and GB method in 20 lots of health food were consistent with each other in BPW positive control group, and the percentage accounted for 95.2% of the total. Conclusion VIDAS method could be used to fast detect the Salmonella in health food. It took 96 h at least to detect Salmonella by GB method. However, it took only 48 h by VIDAS method. It could be the final result if the result was negative, and it should be confirmed according to the GB method if the result was positive. KEY WORDS: health food; Salmonella; automated immunoassay analyzer; fast detection 1 引言 GB16740-1997 ( ) [1],, GB4789.4-2010 [2], 4 (96 h) VIDAS( ) (ELFA), (Solid Phase Receptacle),,, -4-370 nm,, * 通讯作者 :,,, E-mail: sijinw@163.com *Corresponding author: WANG Si-Jin, Responsible Pharmacist, Beijing Institute for Drug Control (Beijing Center for Health Food and Cosmetics Control), No.13, Shuiche Hongtong, Xicheng District, Beijing 100035, China. E-mail: sijinw@163.com
678 6,,, VIDAS 2 (48 h) [3,4], VIDAS, VIDAS,, 2 材料与方法 2.1 仪器与试剂 VIDAS( ) (SLM- Ⅱ ), (VITEK2- Compact)); (BPW) (TTB), (SC), (BS), (XLD) ; 2.2 样品 1, 21 2.3 方法 GB4789.4-2010,, (BPW), (CMCC(B)50094)50~100 CFU(BPW ) GB4789.4-2010, (TTB) 24 h, VIDAS 3 结果与分析 2, 21 GB VIDAS, 21 BPW, GB 5, 表 1 样品列表 Table 1 The sample list 1 71 A 20131001 K 2 77 B 2013-09-27 3 87 C 3338H7P 6 4 88 D TAG0360 5 89 E 20130011 6 90 F 130705 7 94 G 20131112E 8 96 H 20131219 9 98 I 20131127 K05A 10 102 J 9678568-01 11 103 K 13K22B01B 12 104 L JA120901207 13 105 M 131001 14 107 N 13051501 15 108 O DJ125 16 109 P 13010101 17 114 Q TAK0563 18 162 R Z3A131 19 178 S 2013.12.06 20 183 T 20131006E 21 195 U 213012
2, : VIDAS 679 Fig. 1 1 Flow chart for detection of Salmonella in health food 16 ; VIDAS 4, 17, 20, 4, 16, 1, 77( B) 96( H) 109( P) 114( Q), 4, 4, BPW 3,,, [5-8] : 4, B [9], H [10] [11], P [12], Q [13] BPW, 1, 104( L), GB ( ), VIDAS,, VIDAS,, VIDAS [14-16] VIDAS, GB GB,,,,,, 21, VIDAS 48 h, GB 96 h~120 h, VIDAS
680 6 Table 2 表 2 保健食品 BPW 阳性对照沙门氏菌检验结果 Results of detection of salmonella (positive control) in health food BPW BPW GB VIDAS GB VIDAS 1 71 - - + + 2 77 - - - - 3 87 - - + + 4 88 - - + + 5 89 - - + + 6 90 - - + + 7 94 - - + + 8 96 - - - - 9 98 - - + + 10 102 - - + + 11 103 - - + + 12 104 - - - + 13 105 - - + + 14 107 - - + + 15 108 - - + + 16 109 - - - - 17 114 - - - - 18 162 - - + + 19 178 - - + + 20 183 - - + + 21 195 - - + + 4 结论 21, 20 VIDAS GB, 95.2%, VIDAS, 48 h,, GB 参考文献 [1]. GB 16740-1997 ( ) [S]. :, 1997. The people's Republic of China Technology Supervision Bureau. GB16740-1997 General standard for health (functional) foods [S].Beijing: Standards Press of China, 1997. [2]. GB 4789.4-2010 [S]. :, 2010. Minister of Health of the People's Republic of China. GB 4789.4-2010 National food safety standard Food microbiological examination: Salmonella[S]. Beijing: Standards Press of China, 2010. [3],. mini-vidas GB [J]., 2003, 22(1): 50 52. Huang L, Meng DL. A comparative test of using Mini-Vidas and GB method inspect Slamonlla spp. for the food [J]. J Xinjiang Norm Univ (Nat Sci Edit), 2003, 22(1): 50 52. [4],,,. [J]., 2010, 38(10): 5320 5323. Huang CJ, Huang XR, Zheng J, et al. Detection of Salmonella in food samples by VIDAS method [J]. J Anhui Agri Sci, 2010, 38(10): 5320 5323. [5],,,.
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