Microsoft Word 钟玮-二校

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1 7 7 Vol. 7 No Journal of Food Safety and Quality Jul., 2016 * 钟玮, 张宁, 刘玉萍, 范一灵, 杨美成 (, ) 摘要 : 目的方法, (RiboPrinter ) 结果 CODE 1~9, CODE 10 Z 2; 10 CFU/g, 10 结论关键词 : ; ; ; ; Proficiency testing results and analysis of quantitative detection of Staphylococcus aureus in food ZHONG Wei, ZHANG Ning, LIU Yu-Ping, FAN Yi-Ling, YANG Mei-Cheng * (Shanghai Institute for Food and Drug Control, Shanghai , China) ABSTRACT: Objective To improve the detection capacity of Staphylococcus aureus in food. Methods Detection was followed the instruction of the proficiency testing, and RiboPrinter was used for identification and ribotyping of the bacteria added into the blind sample. Results Staphylococcus aureus was detected in CODE 1~9 and there was no Staphylococcus aureus in CODE 10. The Z scores of the positive samples were all less than 2 and the result of the negative sample was less than 10 CFU/g. The test results of 10 samples were satisfied. Conclusion The laboratory detection capability has been improved by implementing proficiency testing. KEY WORDS: Staphylococcus aureus; quantitative; proficiency testing; identification; ribotyping 1 引言 (Staphylococcus aurues, S. aurues),,,,, 30%~80%, [1] [2],,, [3], 2015 * 通讯作者 :,,, [email protected] *Corresponding author: ANG Mei-Cheng, Ph.D., Chief Pharmacist, Shanghai Institute for Food and Drug Control, Shanghai , China. yangmeicheng@ vip.sina.com

2 7, : 2759 [4], 2 材料与方法 2.1 材料试剂和仪器, 10, CODE 1~10, ; 10, 25 g, CODE 1~10 1 (, ); Baird-Parker ( BD ); ( ); ( ); - (BHI)( ); ( ); ( ); RiboPrinter ( Qualicon ) RiboPrinter ( Qualicon ) 2.2 实验方法金黄色葡萄球菌定量检验,, 225 ml,,, 10 GB Baird-Parker [5],, 10, 1 ml ml Baird-Parker, L,, (36±1) 45~48 h,, GB Baird-Parker, 2 GB , Baird-Parker,, 3 20~200 CFU, [5] 盲样中添加菌的鉴定及分型 (1) [6] EcoR Ⅰ, RiboPrinter, 40 μl, 30μL, 5 μl A B, RiboPrinter,, DuPontID, (2) RiboPrinter BioNumerice (Version 6.6), Pearson, (unweighted pair-group method with arithmetic means, UPGMA), 3 结果与讨论 3.1 金黄色葡萄球菌定量检测 10, 3 1, Baird-Parker,, [5], -20,, Baird-Parker, 10,,,, --, GB , 3, Baird-Parker,,, 48 h,, [7], ;,,,, 18~24 h, ;, [8] Baird-Parker,, [9,10],,, [11]

3 表 1 金黄色葡萄球菌定量检测平板计数结果 Table 1 The counting results of S. aureus (CFU/g) Baird-Parker CODE Baird-Parker CODE Baird-Parker CODE Baird-Parker CODE Baird-Parker CODE Baird-Parker CODE Baird-Parker CODE Baird-Parker CODE Baird-Parker CODE Baird-Parker CODE

4 7, : 2761, CODE 1~9, CODE 10, Baird-Parker Z 2; 10 CFU/g, 3.2 盲样中添加菌株的鉴定及分型, CODE 1~10, 4 ( 2 ),, CODE , RiboPrinter, 1, 8, 4, #2 #8 (S. warneri) #6 #7 (S. epidermidis) #4 #5 (S. aureus) #1 #3 (S. simulans) EcoR Ⅰ, rdna, EcoRⅠ 8, 1.1~48 kb rdna, 8 4, 3.0%~91.8%, 2 S. aureus(#4 #5) 91.8%,, 3,,, 2 (#1 #3) 74.6%; 2 S. epidermidis (#6 #7) 70.7%; 2 S. warneri (#2 #8) 62.5%,,,, 4 结论, Z 2, Baird-Parker,, Baird-Parker,, ;,,,,, Baird-Parker Baird-Parker,,,,,,, 1, 1, GB Baird-Parker 2 3, Baird-Parker 1 RiboPrinter Fig. 1 Dendrogram of RiboPrinter clusters and relationships of adding bacteria in the sample

5 2762 7,,, [12], [13], :, RiboPrinter, [14],, [15] ;, ;, [16] ;,,,,,, 参考文献 [1] Atanassova V, Meindla R. Prevalence of Staphylococcus aureus and Staphylococcal enterotoxins in raw pork and uncooked smoked ham-a comparison of classical culturing detection and RFLP-PCR [J]. Int J Food Microbiol, 2001, 68(1-2): [2],,,. [J]., 2014, 41(12): Huang Q H, Zhang Z J, Chai D C, et al. Analysis of results from several detection methods in a microbiological proficiency testing [J].Guangdong Chem Ind, 2014, 41(12): [3]. [J]., 2011, (6): Zhen Z. The analysis of microbiological proficiency testing results [J]. Heilongjang Agric Sci, 2011, (6): [4] CNAS-CL09: 2013 [Z] [8] Silva BO, Caraviello DZ, Rodrigues AC, et al. Evaluation of petrifilm for the isolation of Staphylococcus aureus from milk samples [J]. J Dairy Sci, 2005, 88(8): [9]. [J]., 2012, 17(3): Li MX. The experience of plasma coagulase test quality control [J]. Port Health Control, 2012, 17(3): [10],,,. [J]., 2005, 15(5): Liu ZZ, Lin T, Li GW, et al. The influence of different culture medium and reaction conditions on plasma coagulase test of Staphylococcus aureus [J]. Inspect Quarant Sci, 2005, 15(5): [11],,,. CNCA-12-A01 [J]., 2013, 23(4): Ji YL, Guo YF, Wang JH, et al. Analysis on results of food microbiological proficiency Test [J]. J Pub Health Prev Med, 2013, 23(4): [12]. / [J]., 2007, 22(4): Wang LZ. Discussion on questions concering experimental comparative and capability verification tests in different laboratories [J]. J Occup Health Damag, 2007, 22(4): [13],,,. [J]., 2013, (2): Lu Y, Wang F, Jin X, et al. Analysis on results of food microbiological proficiency test (CNAS T0504) [J]. J Inspect Quarant, 2013, (2): [14]. [J]., 2009, (2): Chen H. Talk about the experience of attending proficiency testing in food microbiological examination [J]. Chin J Health Lab Technol, 2009, (2): [15],,,. [J]., 2014, 26(3): Shu JJ, Zhang WC, Yuan F, et al. Proficiency testing in food microbiological examination [J]. Shanghai J Prev Med, 2014, 26(3): [16],,,. [J]., 2014, 5(10): Xiao J, Li HQ, Chen K, et al. Detection methods of aerobic plate count on food microbiological proficiency testing [J]. J Food Saf Qual, 2014, 5(10): ( 责任编辑 : 姚菲 ) CNAS-CL09:2013 Guidance on the application of testing and calibration laboratory competence accreditation criteria in the field of microbiological testing [Z]. 作者简介 [5] GB [S]. GB Food microbiological examination Staphylococcus aureus [S]. [6],. [J]., 2015, 12(6): 钟玮, 硕士, 药师, 主要研究方向为微生物学检验 [email protected] Jiang ZJ, Gao C. Proficiency testing results and analysis of detection ability of S. aureus and P. aeruginosa in cosmetics [J]. Flav Frag Cosmet, 2015, 12(6): [7],,,. [J]., 2007, 17(6): Huang RT, Wu QP, Zhang JM, et al. Advance in Staphylococcus arueus 杨美成, 博士, 主任药师, 主要研究方向为实验室质量管理药物分析与微生物学检验 [email protected] chromogenic media research [J]. Chin J Health Lab Tech, 2007, 17 (6):

微生物应用说明

微生物应用说明 CNAS-CL26 检测和校准实验室能力认可准则在感官检验领域的应用说明 Guidance on the Application of Testing and Calibration Laboratory Competence Accreditation Criteria in the Field of Sensory Testing 中国合格

Microsoft Word 钟 玮-二校

Microsoft Word 钟玮-二校