分子生物 騰達行企業股份有限公司 UNIMED HEALTHCARE INC Q3 July-September

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1 分子生物 騰達行企業股份有限公司 UNIMED HEALTHCARE INC Q3 July-September qpcr Products Sensitivity Specificity Trustworthiness 本期焦點第 3 代 Tet-On 3G ~ mrnaexpress ~ µg target mrna FlashGel System ~ 5 Protease & Phosphatase inhibitor Cocktail ~

2 Leu Asn Ile Ala Glu Leu Asn U 熱賣產品 TALON & His TALON Co 純度 TALON,Leakage His tag, ( ) Native purification 純化量 價格 結合力 還原劑耐受力 His60 Ni 純度 Chelating, Leakage Binding capacity ~60 mg/ml FPLC/ Gravity 純化量 結合力 Cat No Product Size TALON Metal Affinity Resin 10 ml TALON Metal Affinity Resin 25 ml His60 Ni Superflow Resin 10 ml His60 Ni Superflow Resin 25 ml 價格 還原劑耐受力 Glu Phe Gln Gly Ala Asn Arg Asp Cys Ile Trp Pro Leu Tyr Ser Met Lys Ala Ile 蛋白質純化 Protein Purification Gln Asp New 騰達行企業股份有限公司 UNIMED HEALTHCARE INC. 本輯收錄 台北 TEL (02) 嘉義 TEL (05) 新竹 TEL (03) 台南 TEL (06) 台中 TEL (04) 高雄 TEL (07) Ile Tyr Pro Purify His tagged protein - Highest Purity TALON - Highest Capacity His60 Phe Ser 分子生物試劑類蛋白免疫試劑類細胞組織試劑類臨床檢查試劑類 分析培養耗材類自動化分析儀類 Ala Asn Leu Purify GST tagged protein Enrich Phosphoprotein/ Phosphopeptide Enrich Glycoprotein Purify Flag tagged protein 騰達行客服專線 歡迎索取 蛋白質純化產品完全手冊!

3 2012Q3 July-September 從準備 RNA 至 qpcr 實驗的全方位試劑 2 P3! Tli RNase H SYBR Premix Ex Taq DNA Polymerase RNA Template preparation qpcr Premix for SYBR/Probe Detection S/N qpcr Dual-Labeled Probes One Step qrt-pcr Reagents qpcr Array 88 pathway-specific gene primers 3 Tet-On 3G 6 P µg target mrna mrnaexpress mrnaexpress ~ µg target mrna 10 FlashGel System ~ 5 12 快速溫和萃取不失活蛋白 Buster Protein Extraction Reagent ~ 16 蛋白萃取的好幫手 ~Protease & Phosphatase inhibitor Cocktail 19 P12 FlashGel 5 Stirred Cells ~ 21 Pearl Impulse P16 Buster Protein Extraction Reagent AlphaImager 2011 UNIMED HEALTHCARE INC. All Rights Reserved

4 8 Takara Your one-stop shop of qpcr 樣品製備 CellAmp Direct RNA Prep Kit (Cat # 3732, 3733) Probe qpcr SYBR qpcr One Step One Step PrimeScript RT- PCR Kit (Cat # RR064A/B) Two Step cdna Synthesis PrimeScript RT Reagent kit (Cat # RR037A/B) PrimeScript RT Master Mix, 5X (Cat # RR036A/B) Two Step cdna Synthesis PrimeScript RT Reagent Kit with gdna Eraser (Cat # RR047A/B) One Step One Step SYBR PrimeScript RT-PCR Kit II (Cat # RR086A/B) Probe qpcr Premix Premix Ex Taq(Probe qpcr) (Cat # RR390A) SYBR Green qpcr Premix SYBR Premix Ex Taq DNA Polymerase II (Cat # RR820A) July - September Q3 限時優惠 SYBR Premix Ex Taq DNA Polymerase (Cat # RR420A) RT Kit One Step qrt-pcr Kit Probe / SYBR qpcr Premix (Cat # RR390A/ RR820A/ RR420A) 原價 13,000 限時特價 8,160 買 5 組 全面 送 折 1 組 02 台北 新竹 台中 嘉義 台南 高雄 服務專線

5 第 1 章 準備 RNA Template for qrt-pcr 10, DNase I, genomic DNA Cell lysate SYBR/Taqman qpcr Cell lysate (at -80 ) 另有 For qpcr and Protein Analysis 版本 : Cell lysate 適用 qpcr 及 Western blot Product Cat No Size CellAmp Direct RNA Prep Kit for Rxns RT-PCR (Real Time) CellAmp Direct Prep Kit for Rxns qpcr and Protein Analysis 操作流程 Adherent Non-adherent cultured cells in cultured cells 96-well plate CellAmp Washing Buffer CellAmp Processing Buffer/DNase I, 5 min Cell lysate PCR tubes, 75 5 min Cell lysate for qpcr Takara 第 2 章 選擇 qpcr Premix for SYBR/Probe Detection 全新配方! 添加 Tli RNase H 避免產生 mrna/cdna hybrids 2 X premix, qpcr RNase Hot start version: :100 copies, (Ct ), qpcr RNaseH 作用示意圖 RT Template mrna, cdna Current PCR Reagent mrna / cdna Hybrid qpcr New Products Tli RNaseH Plus cdna mrna, qpcr 提供最高的專一性 SYBR Premix Ex Taq DNA Polymerase II (Tri RNaseH Plus, Cat # RR820A), RR820,GCrich template! 30! July - September Q3 台北 新竹 台中 嘉義 台南 高雄 服務專線

6 Takara 提供最佳的放大效率 SYBR Premix Ex Taq DNA Polymerase (Tri RNaseH Plus, Cat # RR420A) cycles (Ct ),, PCR,, 500 bp RR420 primer dimer,qpcr! GC-rich template! 適合 TaqMan qpcr Premix Ex Taq DNA Polymerase (Tri RNaseH Plus, Cat # RR390A) 2 X master mix, fast qpcr ABI PRISM 7000/7700 ABI 7300/7500/7500 Fast Real-Time PCR System LightCycler 480 ABI StepOne/StepOnePlus Real-Time PCR System 適用多種機型 CFX96/MyiQ2 Thermal Cylcer Dice Real Time System I Smart Cycler II System Product Cat No Size SYBR Premix Ex Taq DNA Polymerase II (Tli RNaseH Plus) RR820A 200 Rxns (5 ml) SYBR Premix Ex Taq DNA Polymerase (Tli RNaseH Plus) RR420A 200 Rxns (5 ml) Premix Ex Taq (Probe qpcr, Tli RNaseH Plus) RR390A 200 Rxns (5 ml) 特別介紹 選擇最適合您的 Dual-Labeled Probes for qpcr Biosearch 研發出全新的 quencher - BHQs (Black Hole Quencher ), 波長吸收範圍更廣泛 (430 nm-730 nm) 由於 BHQs 本身不產生螢光, 能有效降低背景值, 並提高能量轉移的效能, 因此成為最常使用的 quencher Emission Spectra of Fluorophores for Multiplexing 多種 reporter dyes 可供選擇高品質反式 HPLC 純化 適用各種機型 July - September Q3 Biosearch, dual-labeled probes support/applications/realtimedesign-software.aspx Ex (nm) Em (nm) DLO-RFB-5 ValuProbe, 5' FAM/3' BHQ-1 50 nmol 10 nmol 5, DLO-COB1-25 5' CAL Fluor Orange 560/3' BHQ-1 25 nmol 5 nmol 7, DLO-CAB2-25 5' CAL Fluor Red 610/3' BHQ-2 25 nmol 5 nmol 7, DLO-Q6B2-25 5' Quasar 670/3' BHQ-2 25 nmol 5 nmol 7, 台北 新竹 台中 嘉義 台南 高雄 服務專線

7 第 3 章 cdna Synthesis:PrimeScript RT Reagent Kit (Perfect Real Time) :37 15, cdna :picogram RNA : oligo dt primer random hexamer dntps RNase inhibitor 5X RT master mix 版本 : RNA template,! gdna Eraser 版本 : 2 genomic DNA,! Takara gdna Eraser (-) gdna Eraser (+) 利用 gdna Eraser 去除 genomic DNA 干擾! After performing a genomic DNA elimination reaction with (+) and without (-) gdna Eraser using 2 µg total RNA isolated from Mouse Liver, the residual amount of genomic DNA in the total RNA sample was analyzed by performing the reversetranscription reaction in the absence of RTase. Three sets of reactions were performed for each case. Product Cat No Size PrimeScript RT Master Mix, 5X (Perfect Real Time) RR036A/B 200/800 Rxns PrimeScript RT Reagent Kit (Perfect Real Time) RR037A/B 200/800 Rxns PrimeScript RT Reagent Kit with gdna Eraser (Perfect Real Time) RR047A/B 100/400 Rxns 第 4 章 One Step qrt-pcr Reagents : qrt-pcr :picogram RNA : hot start Ex Taq, Product Cat No Size One Step PrimeScript RT-PCR Kit (Perfect Real Time) RR064A/B 100/500 Rxns One Step SYBR PrimeScript RT-PCR Kit II (Perfect Real Time) RR086A/B 100/500 Rxns 第 5 章 qpcr Array - Takara PrimerArray :, KEGG, 88 pathway-specific gene 8 housekeeping gene Pre-validated primers (melting curve, single band on electrophoresis), well premix forward & reverse PCR primer (2.5 µm, 50 µl) : well 12 qpcr (in 96-well format) Product Size Cat No (for human) Cat No (for mouse) PrimerArray Cytokine-Cytokine Receptor Interaction 12 Rxns PH001 PN001 PrimerArray Cell Cycle 12 Rxns PH002 PN002 PrimerArray Cell Adhesion Molecules 12 Rxns PH003 PN003 PrimerArray Jak-STAT Signaling Pathway 12 Rxns PH004 PN004 PrimerArray Natural Killer Cell Mediated Cytotoxicity 12 Rxns PH005 PN005 PrimerArray Axon guidance 12 Rxns PH006 PN006 PrimerArray Focal adhesion 12 Rxns PH007 PN007 PrimerArray T Cell Receptor Signaling Pathway 12 Rxns PH008 PN008 PrimerArray TGF-beta Signaling Pathway 12 Rxns PH009 PN009 PrimerArray Wnt Signaling Pathway 12 Rxns PH010 PN July - September Q3 台北 新竹 台中 嘉義 台南 高雄 服務專線

8 Clontech 第 3 代 Tet-On 系統 Tet-On 3G inducible expression system No Dox Dox Clontech Tet-On 3G 系統, 藉由 doxycycline (Dox) 存在與否, 調控基因表現 When Dox binds, the transactivator undergoes a conformational change allowing it to bind tet operator (teto) repeats within the TREG Promoter (PTRE3G). The transactivator activates expression through transcription activation domain repeats. Tet-on 3G 改良 Transactivator 及 Inducible promoter, 大幅降低背景值並提高對 Dox 的靈敏度 Tet-On 3G 背景值更低 Tet-On 3G 對 Doxycycline 的靈敏度提高 Tet-on 3G 大幅提高基因誘導表現量 July - September Q3 06 台北 新竹 台中 嘉義 台南 高雄 服務專線

9 利用 Tet-on 3G bicistronic 系統, 同時誘導兩種基因表現 Clontech ptre3g-ires expressing both mcherry, from MCS I, and luciferase, from MCS II (Panel A), was cotransfected with pcmv-tet3g into HeLa cells with increasing levels of Dox. Induced expression of mcherry was monitored by fluorescent microscopy (Panel B) and luciferase expression was measured using a luciferase assay (Panel C). Tet-On 3G EF1α promoter 系統, 適用於幹細胞 ptre3g-bi inducible bidirectional promoter 能同時誘導等量的兩種基因 24 hr cotransfection Dox ( 100 ng/ml ) Dox ( 0 ng/ml ) Mouse embryonic stem cells (ES-E14TG2a mes cells) were cotransfected with ptre3g-zsgreen1 and pef1α-tet3g using Xfect Stem transfection reagent. The stem cells show ZsGreen1 expression only in the presence of Dox (Panel C). Cat No Product Promoter of Regulatory Plasmid Tet-On 3G Inducible Expression System CMV Tet-On 3G Inducible Expression System (EF1α Version) EF1α Tet-On 3G Bidirectional Inducible Expression System CMV Tet-On 3G Bidirectional Inducible Expression System (EF1α Version) EF1α Tet-On 3G Bidirectional Inducible Expression System (with ZsGreen1) CMV Tet-On 3G Bidirectional Inducible Expression System (EF1α, ZsGreen1) EF1α Tet-On 3G Bidirectional Inducible Expression System (with mcherry) CMV Tet-On 3G Bidirectional Inducible Expression System (EF1α, mcherry) EF1α Tet-On 3G Inducible Expression System (Bicistronic Version) CMV Tet-On 3G Inducible Expression System (EF1α, Bicistronic) EF1α Tet-On 3G Inducible Expression System (with ZsGreen1) CMV Tet-On 3G Inducible Expression System (EF1α, ZsGreen1) EF1α Tet-On 3G Inducible Expression System (with mcherry) CMV Response Plasmid July - September Q Tet-On 3G Inducible Expression System (EF1α, mcherry) EF1α 台北 新竹 台中 嘉義 台南 高雄 服務專線

10 2012. July - September Q3 Clontech Clontech Takara Fast to set up no need to create a Tet-On or Tet-Off cell line Fast to induce reach 80% of maximal expression in just 2 hours Doxycycline-free protocol instead just add Tet-Express transactivator directly to cells Tet-On / Tet-Off, : Stable cell lines, Clontech Tet-Express, Tet-Express Tet-Off, Tet-Express 1;, Tet response element 2 ( 1-2 ), : Tet-Express, Tet-On / Tet-Off, P TRE3G Tet response element ( ) 7 teto, Tet- Express ;,P TRE3G, Minimal CMV Promoter,, P TRE3G Tet-Express, Tet-Express rapidly induces gene expression from TRE promoters. HeLa cells containing a stably integrated luciferase expression cassette under the control of a TRE promoter were treated with Tet- Express and compared to untreated cells from the same HeLa cell culture at different time points. 88% of maximal expression was achieved in just 2 hr. Fast Tet-Express 添加後 2 小時即能誘導出最大表現量的八成 ptre3g 背景值最低 Simple Tet-Express Inducible Expression System 1 2 Basal expression from the ptre3g promoter is undetectable by Western analysis. HeLa cells containing a stably integrated tetracycline inducible luciferase expression cassette were treated with Tet-Express according to the standard protocol and incubated overnight in parallel with untreated cells from the same HeLa cell culture. The next day, lysates of treated and untreated cells were prepared and blotted with antiluciferase and anti-actin antibodies. 08 台北 新竹 台中 嘉義 台南 高雄 服務專線

11 Tet-Express 這樣作! Hygromycin or puromycin selection -Tet-Expess GOI OFF GOI ON Clontech Clontech Takara +Tet-Expess Tet-Express 適用於各種 cell line Tet-Express works well with a broad range of cell types. Cultures of 293T cells, HeLa cells, MCF7 cells, and HepG2 cells were transfected with a plasmid that expresses luciferase under the control of a TRE promoter. The next day, cells from the four transfected cell cultures were treated with Tet-Express according to the standard protocol and incubated overnight in parallel with untreated cells from each transfected culture. On the following day, treated and untreated cells were assayed for luciferase activity. Add Tet-Express 利用 Tet-Express 系統, 同時誘導兩種基因表現 ZsGreen1 and mcherry fluorescent proteins were cloned into separate multiple cloning sites that flank the inducible bidirectional promoter in the ptre3g-bi vector. The plasmid was then transiently transfected into HEK 293 cells. After 24 hr, the cells were treated with the Tet-Express reagent according to the protocol, and induced expression of the fluorescent proteins was observed 24 hr later, using fluorescence microscopy. No Tet-Express Cat No Product Response Plasmid Tet-Express Inducible Expression System Tet-Express Inducible Expression System (Bidirectional Version) Tet-Express Inducible Expression System (Bidirectional, ZsGreen1) Tet-Express Inducible Expression System (Bidirectional, mcherry) Tet-Express Inducible Expression System (Bicistronic Version) Tet-Express Inducible Expression System (with ZsGreen1) Tet-Express Inducible Expression System (with mcherry) Cat No Product Size Tet-Express 25 Rxns Tet-Express 100 Rxns July - September Q3 台北 新竹 台中 嘉義 台南 高雄 服務專線

12 2012. July - September Q3 SBI mrnaexpress TM mrna Synthesis Kit Transcribe and deliver mrna into cells for instant expression : mrnaexpress, µg target mrna : mrna,5 cap analog,3 poly-a tail, mrna :mrnaexpress mrna :mrna (SBI RNAFection), 操作流程 Sample prorein expression from mrnaexpress Transcripts Autofluorescence(GFP) and immunostaining of the factors are shown in freen and blue is DAPI staining for nuclei. Dose respose of GFP mrnaexpress Transcripts HEK293 cells transfected with increasing amounts of modified GFP mrnaexpress. mrnaexpress 好幫手 RNAFection mrna 20 mrnaexpress mrna Synthesis kit MR-KIT-1 5 rxns RNAfection RNA transfection reagent MR750A-1 50 ul 10 台北 新竹 台中 嘉義 台南 高雄 服務專線

13 mrnaexpress 應用 1 mrna ipsc Reprogramming , Cell Stem Cell mrnas, ips cells (Cell Stem Cell 2010 (7): 1-13) : 1), genome 2) 3) SBI mrna Reprogramming Daily transfection Colony picking Expansion & Validation D1 OSKML at stoichiometry of 3:1:1:1:1 D18 D21 D :1:1:1:1:1, Oct4, Sox2, Klf4, c-myc, Lin28, GFP mrnas 2. mrnas (2 µg per 6-well), RNAFection 3., 18 3, 21,, ips cells mrnaexpress 應用 2 Transdifferentiation Factors Recent History of Transdifferentiation, transdifferentiation (TD) SBI TD factors, modified mrna lentivector, (ex: ips cells) Human Oct4 mrna MR100A-1 / MR100A-2 10 / 20 ug Human Sox2 mrna MR200A-1 / MR200A-2 10 / 20 ug Human Klf4 mrna MR300A-1 / MR300A-2 10 / 20 ug Human c-myc mrna MR400A-1 / MR400A-2 10 / 20 ug Human Lin28 mrna MR500A-1 / MR500A-2 10 / 20 ug Human Nanog mrna MR600A-1 / MR600A-2 10 / 20 ug Human MyoD mrna MR900A-1 / MR900A-2 10 / 20 ug GFP mrna, Positive control MR700A-1 / MR700A-2 10 / 20 ug mnaexpress RFP transcript MR800A-1 / MR800A-2 10 / 20 ug 台北 新竹 台中 嘉義 台南 高雄 服務專線 July - September Q3 11

14 FlashGel system for DNA Markers run on a 1.2% FlashGel Cassette, well format, 275 V for times as shown. Sample lanes from left to right: FlashGel DNA Marker (100 bp 4 Kb), FlashGel QuantLadder, Lonza bp Marker, Lonza 100 bp Ladder. Lower voltage will improve separation of fragments >4Kb. Lonza FlashGel System 神乎其技 ~ 意想不到的快速跑膠只要 5 分鐘 FlashGel System DNA DNA, UV, DNA migration, , 5 1, DNA, 快速 ~ 5 分鐘完成 DNA 電泳 Separation at various run times on the FlashGel System FlashGel ( 275V ) 10 bp ~4kb DNA 2~7, 30, band July - September Q3 高靈敏度與高解析度 ~ 絕佳的敏感度 Comparison of FlashGel System and E-Gel separations 1.2% FlashGel DNA Cassette, well format. 275 V, 7 minute run on the FlashGel Dock. 簡單操作 ~5 大步驟, 即可完成實驗 1,2% E-Gel, 12-well format. 30 minute run on the E-Gel PowerBase v.4. Cassette Dock Load samples Lanes 1, 7 & 13: FlashGel DNA Marker; Lanes 2 & 8: FlashGel QuantLadder; Lanes 3 & 9: Lonza 100 bp Ladder; Lanes 4 & 10: Lonza 50-2,500 bp Marker; Lanes 5 & 11: 285 bp ß-Actin PCR; Lanes 6 & 12: 294 bp Ambion control PCR. Samples diluted with 1X FlashGel Loading Dye prior to loading. Dilutions and load volumes optimized for each sample in each gel system., 12 台北 新竹 台中 嘉義 台南 高雄 服務專線

15 方便 ~ 即時偵測 Dark Reader, transilluminator, UV Nucleotide FlashGel ;, CCD camera!! DNA bands as viewed during a run on the FlashGel Dock Lonza FlashGel DNA Cassette 2.2%, well, double-tier 彈性大 ~ 可同時跑 34 個 samples Gel samples, 34 samples, Cassette run at 250 V for 5 minutes. Lanes 1, 7 & 13: FlashGel DNA Marker 50 bp 1.5 kb; Lanes 2, 8 & 14: FlashGel DNA Marker 100 bp 3 kb; Lanes 6 & 12: FlashGel QuantLadder; Lanes 3-5, 9-11 & 15-17: 8 ng loads of DNA fragments. FlashGel system for RNA 30min ok, : 30 : < 10 ng RNA : precast agarose gel cassettes, EtBr, FlashGel RNA 1.2%, well EtBr, say No! RNA 系統大評比 ~ FlashGel system for RNA 省事又省時 FlashGel System RNA Buffer preparation Sample preparation Gel preparation Running buffer Time prepare samples in 50% Cat No : formaldehyde and RNase-free Precaset gel RNase-free water 30 min sample buffer water;denature for 5 min at 65 10x running buffer : MOPS, sodium Acetate, EDTA, adjust ph 10x loading buffer : Orange G, Ficoll400 and EDTA Prepare samples in formamide,formaldehyde, running buffer and loading buffer; denature for 10 min at 65C Cassette run at 225 V for 8 minutes, and imaged 20 minutes post-run. Lanes 1,6 & 11: FlashGel DNA Marker 100 bp 4 kb; Lanes 2, 7 & 12: 100 ng Lonza RNA Ladder, Lanes 3, 8 & 13: 100 ng E.coli Total RNA; Lanes 4,9: ~ 100 ng Yeast (S.cerevisiae) Total RNA; Lanes 5 & 10: 100 ng Mouse Thymus Total RNA. agarose in running buffer and RNase-free water. 45, 3.8 ml of 37% formaldehyde, comb 1x Running buffer ~1hr July - September Q3 台北 新竹 台中 嘉義 台南 高雄 服務專線

16 2012. July - September Q3 Clontech Lonza Takara FlashGel system for Recovery FlashGel vs. Column Recover FlashGel Recovery System Separate fragments on FlashGel System 3-5 minutes Column Recovery Method Separate fragments on agarose gel minutes FlashGel Recovery system 只要 2 步驟, 輕輕鬆鬆搞定膠體回收實驗!! 回收率高達 80%~100%, 方便又省時 回收範圍廣 ~50bp 也可輕易回收 DNA size range on the FlashGel System for Recovery 3 ul aliqouts of recoverd samples consisting of 100 ng of fragments ranging from 50 bp to 4 kb separated on a 1.2% FlashGel Excise bands from agarose gel 5-10 minutes 還在為回收 DNA 而奮鬥?! sample loading well, well 回收效率佳 Recover DNA directly from wells 1-5 minutes Recover DNA via spin column minutes Total time: 4-10 minutes Total time: 1 hour 將樣品回收後, 進行第二次跑膠, 結果顯示不僅小片段能夠輕易回收, 且回收效果佳 Load sample here Extract DNA here Recovery efficiency on the FlashGel system for recovery Plasmid DNA (pbr322) was subjected to restriction enzyme double digestion using PstI and BamHI. Samples of the restricted DNA were separated and 3.2 kb fragments were recovered using the FlashGel Recovery System (FG) or spin column kits (C1 and C2). Image 1 compares 5% of each recovered sample. Aliquots of the recovered samples were ligated into PstI/BamHI double digested puc19 vector (V). Samples of the ligation reactions were transformed into E.coli competent cells. The number of colonies obtained with both samples were very similar. Image 2 shows examples of PstI/BamHI cut plasmid samples from two colonies from each sample. V shows a restricted sample of vector with no insert. 14 台北 新竹 台中 嘉義 台南 高雄 服務專線

17 8 FlashGel 系列產品全面折還有輕巧方便的照相系統喔! USB, PC!! 要買要快 Clontech Lonza Takara Cat No: Product FlashGel Dock For use with all FlashGel Cassette types FlashGel Camera Includes: Camera, hood enclosure, USB cable and software installation CD.For use with the FlashGel Dock. FlashGel System Includes: FlashGel Dock; FlashGel Camera; 9 pk FlashGel DNA Cassettes( 1.2%, well ); FlashGel Loading Dye and FlashGel DNA Marker. FlashGel System for DNA FlashGel DNA Cassette 1.2% agarose, well single-tier, 9 pk FlashGel DNA Cassette 1.2% agarose, well double-tier ( 34-well ), 9 pk FlashGel DNA Cassette 2.2% agarose, well single-tier, 9 pk FlashGel DNA Cassette 2.2% agarose, well double-tier ( 34-well ), 9 pk FlashGel Loading Dye 5 x 1 ml, 5X concentration FlashGel DNA Marker, 100 bp - 4 kb 500 µl ready-to-load, band sizes: 100/200/300/500/800/1,500/2,000/4,000 bp. Recommended for 1.2% Cassettes FlashGel DNA Marker, 50 bp kb 500 µl ready-to-load, band sizes: 50/100/150/200/300/500/800/1,500 bp. Recommended for 2.2% cassettes FlashGel DNA Marker, 100 bp - 3 kb 500 µl ready-to-load, band sizes: 100/300/500/800/1,500/3,000 bp. Recommended for double-tier cassettes FlashGel QuantLadder 250 µl ready-to-load, band sizes: 100 bp ( 3 ng ) / 250 bp ( 7.5 ng ) / 400 bp ( 15 ng ) / 800 bp ( 21 ng ) / 1,500 bp ( 30 ng ) FlashGel DNA Starter Kit Includes : FlashGel Dock; 9 pk FlashGel DNA Cassettes 1.2% agarose; well;1 ml FlashGel Loading Dye and 150 µl FlashGel DNA Marker 100 bp 4 kb. FlashGel System for Recovery FlashGel Recovery Cassettes 1.2% agarose, double-tier ( 18-well ), 9 pk FlashGel Recovery Cassettes 2.2% agarose, double-tier ( 18-well ), 9 pk FlashGel Recovery Buffer 2 x 500 µl FlashGel Recovery Starter Kit Includes: 9 pk FlashGel Recovery Cassettes; 1.2% agarose, doubletier( 18-well ); FlashGel Loading Dye; FlashGel Recovery Buffer; FlashGel QuantLadder; Visualization Glasses; Control Fragment. FlashGel System for RNA FlashGel RNA Cassette 1.2% agarose, single-tier, 9 pk FlashGel RNA Cassette 1.2% agarose, double-tier ( 34-well ), 9 pk Formaldehyde Sample Buffer 5 x 1 ml, RNA denaturing sample buffer ( contains bromophenol blue and xylenecyanol ) FlashGel Loading Dye 5 x 1 ml, 5X concentration, RNA native sample buffer FlashGel RNA Marker 50 µg, band sizes: 0.5/1/1.5/3/5/9 kb AccuGENE Molecular Biology Water 1 L, for diluting samples and flooding sample wells, RNase-free FlashGel RNA Starter Kit Includes: 9 pk FlashGel RNA Cassettes 1.2% agarose; well; Formaldehyde,Sample Buffer; FlashGel RNA Marker; and AccuGENE Molecular Biology Water July - September Q3 台北 新竹 台中 嘉義 台南 高雄 服務專線

18 Merck.Millipore BUSTER Protein Extraction Reagent 快速溫和萃取不失活蛋白 ProteoExtract Subcellular Proteome Extraction Kit ( S-PEK ) Cat No kit Fractions ( Cytosolic, Membrane, Nuclear, Cytoskeletal ), Native Proteins, Enzyme Activity Assays,, Culture Plate 1D 2D liquid chromatography ( LC ) protease inhibitor cocktail, Benzonase nuclease DNA RNA Cells were transfected with plasmids encoding either β- galactosidase or Renilla luciferase to be expressed in the cytoplasm. Subcellular extraction was performed and activities of the fractions 1 4 are displayed in arbitrary units using a dedicated commercially available extraction product as positive control July - September Q3 The comparison of fractions 1-4 by twodimensional SDS-PAGE indicates a large number of protein spots that are specific for the respective subcellular fraction. 16 台北 新竹 台中 嘉義 台南 高雄 服務專線

19 ProteoExtract Native Membrane Protein Extraction Kit ( M-PEK ) Cat No Native Membrane Protein, protease inhibitor cocktail, Merck.Millipore HEK293 cells were extracted with buffered 1% Triton X-100 to generate a total lysate or extracted with M-PEK to yield a membrane fraction. The measurements demonstrate a 4.5 fold enrichment of the EGF receptor in the M-PEK-extracted membrane fraction. ProteoExtract Cytosol/Mitochondria Fractionation Kit Cat No. QIA88 Mitochondria Cytosol, protease inhibitor cocktail, 1D 2D Western blotting ELISA Mitochondrial and cytosolic fractions were isolated from HEPG2 cells using QIA88 ProteoExtract Cytosol/ Mitochondria Fractionation Kit. Equivalent amounts (10 µg) of HEPG2 homogenate (total protein), cytosolic, and mitochondrial fractions were analyzed by Western blot for proteins localized to different cellular regions as indicated. Representative results from 2 independent fractionations are shown July - September Q3 台北 新竹 台中 嘉義 台南 高雄 服務專線

20 2012. July - September Q3 Merck.Millipore Clontech Takara 訂購資訊 Cat No BugBuster Products by Cell Type Starting Material Total Culture Cell Pellet Applications 1D PAGE 2D PAGE / IEF Activity Assay E-Coli Master Mix BugBuster Protein Extraction Reagent BugBuster 10X Protein Extraction Reagent PopCulture Reagent YeastBuster Protein Extraction Reagent CytoBuster Protein Extraction Reagent Insect PopCulture Reagent YEAST CytoBuster Protein ProteoExtract Kits Gram-negative bacteria ( E. coli ) rlysozyme Solution Lysonase Bioprocessing Reagent Chicken Egg White Lysozyme Solution Gram-positive bacteria All cells Benzonase Nuclease Comments Combines BugBuster Protein Extraction Reagent with Benzonase Nuclease and rlysozyme Solution. Convenient, all-in-one protein extraction reagent efficiently lyses bacteria and digests nucleic acids. Efficient protein extraction from E. coli under non-denaturing conditions. A concentrated form of BugBuster Protein Extraction Reagent. Ideal for extraction when a specific buffer is required for protein stability. Protein extraction from cells directly in the culture medium; no centrifugation required. Efficient protein extraction from yeast under non-denaturing conditions from any volume of culture. Add 0.5 M THP Solution ( included ) and Benzonase Nuclease for enhanced efficiency. Gentle lysis of insect cells with retention of protein activity for assays and purification. Can use with monolayers or pellets derived from suspension cultures. Lysis of insect cells directly in serumfree medium. Ideal for expression screening of many small samples. Gentle lysis of mammalian cells with retention of protein activity for assays and purification. Can use with monolayers or pellets derived from suspension cultures. Extract protein fractions from different parts of the cell. A range of kits offering maximum flexibility. Cleaves bond in peptidoglycan layer of E. coli cell wall. Convenient mixture of rlysozyme and Benzonase Nuclease minimizes pipetting steps. Cleaves bond in peptidoglycan layer of bacterial cell wall. Degrades all types of nucleic acids for more efficient protein extraction, faster chromatography, and reduced interference in assays. 18 台北 新竹 台中 嘉義 台南 高雄 服務專線

21 BUSTER 好麻吉 Protease & Phosphatase inhibitor Cocktail 蛋白萃取的好幫手,,, Inhibitors Target Proteases,, Merck.Millipore Protease inhibitor Cocktail Stability of Protease Inhibitor Dilutions in BugBuster Protease inhibitors were diluted to the prescribed working concentration. The ability of the inhibitors to inhibit the proteolytic activity of PRONASE was measured by using the Universal HT Protease Assay.The data shows that the efficiency of the cocktail remained higher than the competitor s tablet in this study. Protein Inhibitor Cocktail Set I Cat No AEBSF, Hydrochloride Aprotinin, Bovine Lung E-64, Protease Inhibitor Leupeptin, Hemisulfate EDTA, Disodium Protein Inhibitor Cocktail Set III Cat No AEBSF, Hydrochloride Aprotinin, Bovine Lung E-64, Protease Inhibitor Leupeptin, Hemisulfate Bestatin Pepstatin A Protein Inhibitor Cocktail Set V Cat No AEBSF, Hydrochloride Aprotinin, Bovine Lung E-64, Protease Inhibitor Leupeptin, Hemisulfate Hot Phosphatase inhibitor Cocktail Cat No. Product Target Size SETCN Phosphatase Inhibitor Cocktail Set II ( Dilute: 1:100 ) Acid Phosphatases Alkaline Phosphatase 1 set ( 5x1 ml ) Protein Tyrosine Phosphatases ( PTPs ) MLCN Phosphatase Inhibitor Cocktail Set IV ( Dilute 1:50 ) Alkaline Phosphatase 1 ml Ser/ Thr phosphatases Hot MLCN Phosphatase Inhibitor Cocktail Set V ( Dilute 1:50 ) Acid Phosphatases Alkaline Phosphatase Protein Tyrosine Phosphatases ( PTPs ) Ser/ Thr phosphatases 1 ml July - September Q3 台北 新竹 台中 嘉義 台南 高雄 服務專線

22 2012. July - September Q3 Merck.Millipore Sale Buster Amicon Ultra Inhibitor WB 產品 6 85 新客戶首購舊客戶試用價舊客戶全系列八五折全系列折折一間實驗室限用一個品項, 限用一次 單件 9 折 買 96pk 送 8pk 85 2 件舊客戶全系列八五折折 凡購買 Merck Millipore 任一款 IPVH WBKLS0500 WB PVDF 及 Substrate, 可享 15,000 舊客戶全系列八五折 Buster 全系列 8 折 IPVH WBLUC ,000 13,500 IPVH WBLUR 台北 新竹 台中 嘉義 台南 高雄 服務專線

23 濃縮大體積樣品的利器 Stirred Cells 買 Stirred cell 送 Membrane 一盒 Merck.Millipore, / buffer autoclaved Regenerated Cellulose, ( < 20g/m 2 ) Regenerated Cellulose, Membrane 9 折 DNA Protein Enzymes Viruses antibodies Macromolecules 1 Ultrafiltrate 2 Ultrafiltration equipment 3 Pressure supply 4 Selector valve 5 Inlet 6 Outlet 7 Feed liquid 8 Reservoir 多種體積大小供您選擇 多種分子量大小的膜供您選擇 Stirred Cell Model 8003 Model 8010 Model 8050 Model 8200 Model 8400 Cat. No Cat. No Cat. No Cat. No Cat. No Max. Process Volume, ml Min. Process Volume, ml Membrane Diameter, mm Effective Membrane Area, cm Hold-up Volume, ml Height, cm Base, cm 6 x 6 6 x 6 7 x 7 9 x 9 11 x 11 Weight, kg Maximum operating pressure: 75 psi ( 5.3 kg/cm 2 ) Relief valve setting: 90 psi ( 6.3 kg/cm 2 ) Maximum diafiltration operating pressure: 55 psi ( 3.9 kg/cm 2 ) NMWL (kda) Filter Diameter : Regenerated Cellulose : Polyethersulfone (PES) July - September Q3 台北 新竹 台中 嘉義 台南 高雄 服務專線

24 2012. July - September Q3 LI-COR Dynamic Range 最高 高解析度小動物活體動態影像系統 Pearl Impulse NIR 近紅外光波段最適合活體影像 ( nm) FieldBrite Xi 專利技術提供絕佳的靈敏度 FieldBrite Xi,,, 即時螢光影像動態攝影 0.5, NIR 影像最清晰 動態攝影最即時 800nm autofluorescence FieldBrite Xi 光學設計 Dynamic range (> 6 O.D.) 雙雷射激發光模組 IRDye 800CW PEG 22 台北 新竹 台中 嘉義 台南 高雄 服務專線

25 另有經濟型小動物活體影像系統 ~ Odyssey CLx 近紅外光定量系統含小動物活體影像配件 讓您 One Probe Simplifies Discovery 單一平台提供 in vitro, in vivo 及 ex vivo 分析全功能 歡迎來電洽詢 Pearl Impulse 光學影像研究流程 LI-COR model BrightSite in vivo Ex vivo 經過嚴格測試, 專一性最佳的近紅外光螢光活體影像研究試劑 Ready-to-Use 可立刻開始動物試驗, 不需要基因改造或細胞轉染動物 IRDye 800CW 已通過 FDA 動物毒性測試核准, 可進入轉譯醫學臨床研究 Tumor Imaging 腫瘤攝影 Cell Tracking 細胞追蹤 Brain Imaging 腦部攝影 IRDye 800CW RGD IRDye 800CW 2-DG IRDye 800CW EGF CellVue NIR815 PSVue 794 Apoptosis in the brain Integrin overexpression Increased glucose metabolism EGFR overexpression Lymphatics 淋巴攝影 Metastasis 腫瘤轉移 IRDye 800CW HA IRDye 800CW EGF IRDye 800CW PEG Lymph vessel leading to subiliac lymph modes Vasculature 血管攝影 IRDye 800CW PEG Micro-metastasis to Kidney Structure and Remodeling 骨頭結構及定位 IRDye 800CW BoneTag TM Surface vasculature Tumor vasculature Bone structure and skeletal details July - September Q3 台北 新竹 台中 嘉義 台南 高雄 服務專線

26 2012. July - September Q3 fortebio ForteBio Octet Platform lable-free, real-time BIOMOLECULAR ANALYSIS PROTEINS.PEPTIDES.SMALL MOLECULES 優點 Bio-layer Interferometry (BLI) Technology 全新 BLI 技術, 避免傳統 SPR 方法必需的樣品前處理工作及昂貴實驗成本限制 Disposable / Regenerationable Biosensors 新式感測器設計, 不僅成本更低, 各樣品間完全獨立分析不干擾 Crude sample compatibility 可直接使用未純化樣品 ( 如血清 細胞液 萃取藥物... 等 ) High Throughput 使用 96/384 孔盤進行樣品偵測, 分析量更大更快速, 而且不需任何清洗步驟 Accuracy and Sensitivity 提供高再現性以及高靈敏度數據 Easy to manage 操作管理容易, 無任何流體管路, 樣品絕對不殘留, 儀器完全不阻塞 Sample total recovery 無需任何額外設定動作, 樣品即可完全回收不浪費, 方便接續其他實驗 ForteBio Octet platform 廣泛應用於下列生命科學的領域 1. 新藥開發 Drug Discovery 2. 抗體篩選 Clone Screening 3. 配體垂釣 Ligand Fishing 4. 結合特異性 Binding Specificity 5. 蛋白質定量 Protein Quantization Binding (nm) Binding Rate 量化 Quantitation Concentration measurements Time (sec) Concentration IgG quantitation 分子鑑別 Molecular Recognition 7. 酶反應 Enzyme Reaction 8. 細胞信號傳導 Cell Signal Transduction 9. 免疫抗原性測試 Immunogenicity 10. 疫苗開發 Vaccine Development Measure k on, k off, and K D Yes/No binding 動力學 Kinetics Affinity Measurements Clone Selection / Screening Epitope Binning / Mapping Quick Assay Optimization 24 台北 新竹 台中 嘉義 台南 高雄 服務專線

27 尚有 FluorChem 高階冷螢光影像系統任君選擇 歡迎來電洽詢 AlphaImager 電泳膠片擷取與分析系統 科學分析等級零缺陷 CCD 感應器 16-bit 高灰階度檔案規格 電泳膠片影像清晰準確定量分析 多款機型供選擇最符合實驗需求 AlphaImager EC System 經濟型電動化影像系統 影像超清晰 升級 OK 1 1 2, CCD 5, 2 3, /365nm, AlphaView 4 抗漲價 NT$ AlphaImager Mini System 經濟型迷你影像系統 USB 介面 廣泛應用性 操作超簡單 CCD,USB,,, UV, /365nm, 5 AlphaSnap : > > > >,!! 4 抗漲價 NT$ 抗漲足感心! 膠片影像系統 10 本活動至 / 31 止!!

28 Part I. 最適合您的 PCR Polymerase Part II. Takara 限制酶 & 修飾酶 Part III. PrimeScript RT-PCR 完全攻略 Part IV. RNA 純化試劑 Part V. DNA Ligation Kit /30! PrimeSTAR MAX PrimeSTAR GXL! 藍綠 85 & 3 1 PrimeScript RT 8 RNA Quick-RNA Direct-zol RNA 85 DNA Ligation Kit 原價 8,000 元 6,500 限量特價 元 騰達行企業股份有限公司 UNIMED HEALTHCARE INC. 台北 TEL (02) 嘉義 TEL (05) 新竹 TEL (03) 台南 TEL (06) 台中 TEL (04) 高雄 TEL (07) 分子生物試劑類蛋白免疫試劑類細胞組織試劑類臨床檢查試劑類分析培養耗材類自動化分析儀類 騰達行客服專線