cm 1. 0 cm /2 MS 20 g L -1 8 g L -1 ph 5. 8 ~ ± 3 12 h d ~ 40 μmol m -2 s

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1 Guihaia Mar http / /journal. gxzw. gxib. cn DOI /j. issn J Gu FG Sun BY Su GX et al. Studies on tissue culture and rapid propagation technique of Echinodorus subalatus J. Guihaia * % HgCl 2 8 min + 6 ~ 8 min 24 h 20% 4 1 /2MS + 6-BA 6. 0 mg L d /2MS + NAA 0. 2 mg L d 100% % Q A * Studies on tissue culture and rapid propagation technique of Echinodorus subalatus GU Fu-Gen * SUN Bing-Yao SU Guo-Xing JIANG Ya-Bo ZHOU Feng-Ming CHEN Meng-Fei Medicine Department Suzhou University Suzhou China Abstract The in vitro culture of Echinodorus subalatus was established by using the unelongated stem as s. The method of surface sterilization of s the effects of different plant growth regulators on the multiplication and rooting were investigated. The results showed that 20% sterile s could be obtained by twice surface treatment with 0. 1% HgCl 2 for 8 min and 6-8 min with an interval of 24 h. When cultured on a 1 /2 MS multiplication medium supplemented with 6. 0 mg L -1 of 6-BA for 42 d the test-tube seedling exhibited a multiplication coefficient of For optimal rooting induction on a 1 /2 MS rooting medium supplemented with 0. 2 mg L -1 of NAA for 28 d could produce rooted plantlets with 100% rooting rate and each plantlet generated about 18 roots on average. Rooted plantlets were acclimatized and transferred into outdoor aquatic environment with 100% survival rate. Key words Echinodorus subalatus unelongated stem tissue culture intermittent sterilization Echinodorus subalatus Alismataceae Echinodorus 2004 * BK gufugen64@ 163. com * Author for correspondence

2 cm 1. 0 cm /2 MS 20 g L -1 8 g L -1 ph 5. 8 ~ ± 3 12 h d ~ 40 μmol m -2 s min 1 g L -1 HgCl 2 21 d x + x min 5 8 min 4 ~ 5 1 /2MS + 6-BA 5. 0 mg L -1 + NAA 0. 1 mg L min g L h 8 min + 5 ~ 10 min 24 h 6 ~ 8 min 20% min 8 min d ~ 8 min 24 h d ~ /2MS 4 6-BA mg L -1 NAA mg L ~ cm 6-BA d cm 40 cm 1 5 ~ 8 cm 2 d 30 d 1. 3 Spss v ~ d 6-BA BA BA 2. 0 ~ BA

3 d Table 1 Effects of different sterilization treatments on contamination ratio of Echinodorus subalatus s 21 d after inoculation Sterilization time min No. of No. of contaminated No. of dead No. of survived sterile Percentage of survived sterile % ± 0. 94d ± a ± a ± a ± 1. 25d ± a ± a ± a ± c ± a ± a ± a ± b ± b ± a ± a ± c ± a ± b ± b ± b ± a ± c ± c ± a ± b ± c ± c ± a ± c ± a ± a 2 28 d Table 2 Effects of different hormone concentrations of 6-BA / NAA on axillary bud induction and rooting of Echinodorus subalatus 28 d after inoculation Concentration of growth Average regulators mg L -1 height of 6-BA NAA shoot Multiplication coefficient Average number of root Average length of root cm ± a ± a ± ab ± j ± a ± a ± b ± h ± a ± a ± ab ± e ± a ± a ± ab ± bc ± a ± b ± ab ± i ± a ± b ± 3. 01b ± g ± a ± b ± ab ± e ± a ± b ± ab ± bc ± 0. 49a ± b ± ab ± f ± a ± b ± b ± e ± a ± b ± ab ± cd ± a ± b ± 3. 46ab ± ab ± a ± b ± ab ± de ± a ± b ± ab ± bc ± a ± b ± ab ± bc ± a ± b ± a ± a 23 Days from inoculation to rooting ~ mg L -1 1 /2MS + NAA 0. 2 NAA mg L -1 1 /2MS + 6-BA 2. 0 ~ mg L mg L BA ~ d 7. 7 NAA BA 0. 2 mg L -1 6-BA 0 ~ 4. 0 mg L -1 6-BA 7. 0 mg L -1 6-BA 6-BA NAA

4 /2MS + 6-BA 6. 0 mg L -1 6-BA min 2 ~ ~ 8 min 24 h 1 /2MS + 6-BA 6. 0 mg L d mm 6-BA 4 mm ~ 3 d 14 d 30 d 100% mm 3. 1 Echinodorus osiris E. horemanii /2MS + 1 NAA 0. 2 mg L Continue on page 262

5 M. Pharm Bull Paula B Anabela M Rui M et al Diversity and fruiting pattern of macrofungi associated with chestnut Castanea sativa in Cai J He JQ Research of model plants and native woody ornamental plants in Yaoluoping Natural Reserve J the Tra's-os-Montes region Northeast Portugal J. Fung Ecol 19 Guihaia Chen Y Xu ZG Zhang KH et al The ecological distribution of the macrofungi in Lushan Mountain J. Acta Ecol Sin Demirel K Uzun Y Macrofungi of Agri Province J. Turk J Bot Fadime Y Mustafa I Macrofungi of Degirmangobazy Balikesir J. Turk J Bot Genevieve MG Caroline M David AR et al Diversity and e- cology of epigeous ectomycorrhizal macrofungal assemblages in a native wet eucalypt forest in Tasmania Australia J. Fung Ecol Gregory MM John P Schmit et al Global diversity and distribution of macrofungi J. Biodiv Cons Guo JR Large-Fungus category and its exploitation in Luyashan Nature Reserve J. Shanxi For Sci Technol He YX Li NS Li JH Ecological distribution of macrofungi in Yaoluoping Natural Reserve J. J Biol Kaya A Macrofungi determined in Golbasi Adiyaman District J. Turk J Bot Li SJ Zhu TH Macro-fungi and Standing Tree Rot J. J Sichuan For Sci Tech Miyazaki T Oikawa N Yamada H Studies on fungal polysaccharides. XX. Galactomannan of Cordyceps sinensis J. Chem Peksen A Karaca G Macrofungi of Samsun Province J. Turk J Bot Shang ZH Yao AX Biodiversity and biodiversity protection J. Grass Turf Swapna S Syed A Krishnappa M Diversity of macrofungi in semi-evergreen and moist deciduous forest of Shimoga District- Karnataka India J. J Mycol Pl Pathol WangYX Chen JY Biodiversity Resources Utilization of Macrofungi in Hubei J. Hubei For Sci Technol Yesil OM Yildiz A Contributions to the macrofungi flora of Batsman Province J. F U Fen ve Muhendislik Bilimleri dergisi 檻檻檻檻檻檻檻檻檻檻檻檻檻檻檻檻檻檻檻檻檻檻檻檻檻檻檻檻檻檻檻檻檻檻檻檻檻檻檻檻檻檻檻檻檻檻 184 Continue from page 184 J. Acta Agric Shanghai M. Yang YP Shi YM Tao YW Tissue culture and rapid propagation of Haemanthus albiflos J. 3 J. Plant Physiol Comm Gu FG Wan ZG Yan SY Zhang HM Ji H Xiao XQ et al. Tissue culture and rapid propagation of Myriophyllum verticillatum J. Plant Physiol Tissue culture and rapid propagation of Echinodorus osiris J. Plant Physiol Comm Comm Sun YF Lu RJ Zhou RM et al In vitro culture of ornamental waterweeds

Microsoft Word - A201405-840_1401293463.doc

Microsoft Word - A201405-840_1401293463.doc 5 10 15 中国科技论文在线 # 台湾独蒜兰无菌萌发与植株再生研究卓孝康 1,2, 兰思仁 1,2, 彭东辉 1,2, 吴沙沙 1,2, 陈潇 1,2, 江鸣涛 1,2 1,2**, 张林瀛 (1. 福建农林大学园林学院, 福建福州 350002; 2. 福建农林大学海峡兰花保育