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1 3 5 Vol. 3 No Journal of Food Safety and Quality Oct., 2012 林洪 *, 曲欣 (, ) 摘要 :,,, 关键词 : ; ; The research progress of purine distribution in food LIN Hong *, QV Xin (Food Science and Engineering College, Ocean University of China, Qingdao , China) ABSTRACT: The determination methods of purine are not unified nowadays, so the results of purine detection are discrepant in some foods. In this paper, methods of sample preparation, purine quantification and purine distribution in food were reviewed and analyzed to provide a reference for further development of purine detection technologies. It has an important significance in guiding consumers to a rational diet so that the incidence of gout could be decreased. KEY WORDS: purine; gout; determination 1 引言, 20%,,, [1, 2], 50% [1],, %, 1.36%, % 0.26% [3, 4] [5, 6],,,, [7],,, 2 食品中嘌呤的检测方法 2.1 样品前处理方法,,,, 基金项目 : ( ) * 通讯作者 :,,, : linhong@ouc.edu.cn

266003, China) ABSTRACT: The determination methods of purine are not unified nowadays, so the results of purine detection are discrepant in some foods.

2 374 3 [8] [9], 酸解提取法,,,, (Adenine) (Guanine); (Hypoxanthine), ;, (1 1, v/v), [10] ;,, ; (1 1, v/v) (Xanthine), (5 5 1, v/v/v),, min, 4,, [11-13],,, 超声波提取法,, [14-16], [17],,,,,, 有机溶剂萃取法, Clariana [18] 6 ( ); [19] 75%,,,,, 2.2 食品中嘌呤的定量方法 50, Vischer Hotchkiss, 2000 [19],, 89%, 5%,,,,,, [20, 21] [22], (HPLC) C 18 C 18, C 18, 1,, C 18,, C 18, (RP-ICP); dc 18 RP 18 C 18, 0.02 mol/l, ph, 254 nm, 0.6~1.0 ml/min, 24~30 3 食品中嘌呤含量分布,,, Clifford [28],,

min, 4,, [11-13],,, 2.1.2 超声波提取法,, [14-16], [17],,,,,, 2.1.3 有机溶剂萃取法, Clariana [18] 6 ( ); [19] 75%,,,,, 2.

3 5, : 375 Table 1 表 1 色谱柱在嘌呤检测中的选用情况 The selection of chromatographic column in purine determination Kromasil-C 18 Hyp a : (1:9, v/v) HPLC Alltech Apollo C 18 Hyp : (1:9, v/v) HPLC Kromasil-C 18 Xan b Hyp : (2:98, v/v) HPLC Discovery C 18 Hyp :(8:92, v/v) HPLC μbondapak C 18 Atlantis C 18 Ade c Gua d Ade Gua Ade Gua Waters symmetryshield RP 18 Waters Atlantis dc 18 Ade Gua Waters Atlantis dc 18 : a: ; b: ; c: ; d: Ade Gua :: : (883.5: 100:15:1.5, v/v/v/v) [ : : (997:1.5: 1.5), v/v/v]:(9:1, v/v) (0.02 mol/l, ph 4.6) (0.02 mol/l, ph 4) (0.02 mol/l, ph 3.8) RP-ICP RP-ICP HPLC HPLC HPLC [14] [15] [23] [16] [24] [25] [26] [27],, 3.1 畜禽肉类中嘌呤含量分布 50%,,, 1977, [29] 50 [13, 30], 2,,, [30], 10 min,,,,, (ATP), 3.2 水产品中嘌呤含量分布,, 2, ; A B, ;,, ; 18 6,,,, /,,, ATP (IMP) [11] 60%, 10 min, 10 min,,,, [31-33, 35],,,,,

Waters Atlantis dc 18 : a: ; b: ; c: ; d: Ade Gua :: : (883.5: 100:15:1.5, v/v/v/v) [ : : (997:1.5: 1.5), v/v/v]:(9:1, v/v) (0.02 mol/l, ph 4.6) (0.02 mol/l, ph 4) (0.02 mol/l, ph 3.

4 376 3 表 2 部分食品中嘌呤含量 Table 2 Contents of purine in some foodstuffs (mg/kg) A B [30] [30] [31] [32] [33] [33] 3.3 大豆中嘌呤含量分布 1982, [36] mg/kg, mg/kg Kaneko K [37] HPLC, 219~1725 mg/kg mg/l [38] mg/kg, mg/kg,, 3.4 酒精饮料中嘌呤含量分布 [39],,, [39, 40], [24] mg/l, 2 3 [25] 14 [10] mg/l,, mg/l, HPLC, [22] LC-MS/MS,,,, [27],, 3.5 其他食品中嘌呤含量分布

119 492 21 610 1242 142 372 13 817 1344 693 557 373 252 1875 1640 195 4 96 1935 1197 182 1 90 1470 [30] [30] [31] [32] [33] [33] 3.3 大豆中嘌呤含量分布 1982, [36] 743.1 982.2 0.0 0.0 mg/kg, 1725.

5 5, : 377,, [19] [41], 4 小结,,,,,, 参考文献 [1] Nuki G. Gout[J]. Medicine, 2002, 30(9): [2] Wall GC, Cooper SA. Gout and Hyperuricemia: New Guidelines and Treatments [J]. J Pharm Pract, 2009, 22(1): [3],,,. [J]., 2006, 22(5): [4],,,. [J]., 2011, 27(7): [5] Sundstrom J, Sullivan L, D'Agostino RB, et al. Relations of serum uric acid to longitudinal blood pressure tracking and hypertension incidence[j]. Hypertension, 2005, 45(1): [6] Iseki K, Ikemiya Y, Inoue T, et al. Significance of hyperuricemia as a risk factor for developing ESRD in a screened cohort[j]. Am J Kidney Dis, 2004, 44(4): [7]. [M]. :, [8] Smolenski R, Lachno D, Ledingham S, et al. Determination of sixteen nucleotides, nucleosides and bases using high-performance liquid chromatography and its application to the study of purine metabolism in hearts for transplantation[j]. J Chromatogr, 1990, 527(2): [9]. [D]., [10],,. HPLC [J]., 2008, (2): [11],. [J]., 1997, 24(1): [12] Fan H, Yang FQ, Li SP. Determination of purine and pyrimidine bases in natural and cultured Cordyceps using optimum acid hydrolysis followed by high performance liquid chromatography[j]. J Pharmaceut Biomed, 2007, 45(1): ,,,. 4 [J]., 2008, 36(6): [14],. HPLC [J]., 2006, 20(7): [15]. HPLC [J]., 2007, 34(12): [16],, 喆,. HPLC [J]., 2005, 28(8): [17],,,. HPLC [J]., 2002, 25(10): [18] Clariana M, Gratacós-Cubarsí M, Hortós M, et al. Analysis of seven purines and pyrimidines in pork meat products by ultra high performance liquid chromatography-tandem mass spectrometry[j]. J Chromatogr A, 2010, 1217(26): [19],,,. [J]., 2000, 21(5): [20] Chen G, Chu Q, Zhang L, et al. Separation of six purine bases by capillary electrophoresis with electrochemical detection[j]. Anal Chim Acta, 2002, 457(2): [21] Xu D, Hua L, Chen H. Determination of purine bases by capillary zone electrophoresis with wall-jet amperometric detection[j]. Anal Chim Acta, 1996, 335(1 2): [22],,,. LC-MS/MS [J]., 2010, 26(12): [23],,,. HPLC [J]., 2002, 25(10): [24],,,. [J]., 2006, (9): [25],. [J]., 2009, 07: [26]. [D]., [27],. [J]., 2006, 32(3): [28] Clifford AJ, Riumallo JA, Young VR, et al. Effect of Oral Purines on Serum and Urinary Uric Acid of Normal, Hyperuricemic and Gouty Humans [J]. J Nutr, 1976, 106(3): [29],, 龍. 製塩 [J]. J Jpn Soc Nutr Food Sci, 1977, 30(3): [30],,,. [J]., 2008, 29(7): [31] Piñeiro-Sotelo M, Rodrı guez-bernaldo De Quirós A, López-Hernández J, et al. Determination of purine bases in sea urchin (Paracentortus lividus) gonads by high-performance liquid chromatography[j]. Food Chem, 2002, 79(1): [32] Lou S. Purine Content in Grass Shrimp during Storage as Related

Relations of serum uric acid to longitudinal blood pressure tracking and hypertension incidence[j]. Hypertension, 2005, 45(1): 28-33. [6] Iseki K, Ikemiya Y, Inoue T, et al.

6 378 3 to Freshness[J]. J Food Sci, 1998, 63(3): [33],,,. [J]., 2008, 33(11): ,,,. [J]., 2012, 34(1): [35] Lou S, Chen H, Hsu P, et al. Changes in purine content of tilapia surimi products during processing[j]. Fisheries Sci, 2005, 71(4): [36] 篠,, 龍. 塩対調響 [J]. 栄栄糧, 1982, 35: [37] Kaneko K, Kudo Y, Yamanobe T, et al. Purine contents of soybean-derived foods and selected Japanese vegetables and mushrooms[j]. Nucleosides Nucleotides Nucleic Acids, 2008, 27(6): [38]. [D]., [39] Yamamoto T, Moriwaki Y, Takahashi S. Effect of ethanol on metabolism of purine bases (hypoxanthine, xanthine, and uric acid)[j]. Clin Chim Acta, 2005, 356(1 2): [40] Choi HK, Atkinson K, Karlson EW, et al. Alcohol intake and risk of incident gout in men: a prospective study[j]. The Lancet, 2004, 363(9417): [41] Yamaoka N, Kaneko K, Kudo Y, et al. Analysis of purine in purine-rich cauliflower[j]. Nucleosides Nucleotides Nucleic Acids, 2010, 29(4-6): ( 责任编辑 : 赵静 ) 作者简介林洪,,, : linhong@ouc.edu.cn

[37] Kaneko K, Kudo Y, Yamanobe T, et al. Purine contents of soybean-derived foods and selected Japanese vegetables and mushrooms[j]. Nucleosides Nucleotides Nucleic Acids, 2008, 27(6): 628 630. [38].

1449 1 相对校正因子的相关方法 1.1 内标多控法研究发现, 在中药各成分间存在着一定的比例关系, 只要各成分的量符合该比例关系, 中药的多 [6] 指标质量控制可以依此法

1449 1 相对校正因子的相关方法 1.1 内标多控法研究发现, 在中药各成分间存在着一定的比例关系, 只要各成分的量符合该比例关系, 中药的多 [6] 指标质量控制可以依此法 1448 相对校正因子在中药多指标测定中的应用研究进展 1, 罗祖良 2, 仇峰 1 1,, 韦日伟 2 1,, 覃禹 2, 张坤 1, 覃洁萍 2, 杨美华 1. 北京协和医学院药用植物研究所, 中草药物质基础与资源利用教育部重点实验室, 北京 100193 2. 广